• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.602-605
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• 2001

Low-temperature adaptation and protection for environmental stresses were studied in the gram-negative soil bacterium Bradyrhizobium japonicum 61A101c. B. japonicum was more resistant to alcohol, $H_2O_2$, heat and freezing following a pretreatment at $4^{\circ}C$, resulting in approximately 10 to 1,000 folds increased survival compared to mid-exponential-phase cells grown at an optimal temperature at $28^{\circ}C$. This phenomena relate to the cold shock protein expressed when cells are exposed to a downshift in temperature. To confirm the presence of cold shock protein genes in B. japonicum, a PCR strategy was employed using a degenerate primer set, which successfully amplified a putative csp gene fragment. Sequence analysis of the PCR product(200bp) revealed csp-like sequences that were up to 96% identical to csp gene of S. typhimurium.

• Proceedings of the KSME Conference
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• 2000.11b
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• pp.228-233
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• 2000

The Korean Next Generation Reactor(KNGR) is a Pressurized Water Reactor adopting direct vessel injection(DVI) to optimize the performance of emergency core cooling system(ECCS). In a certain accident, however, pressurized thermal shock(PTS) of the vessel due to the sudden contact with the injected cold water is expected. In this paper, an accident of Main Steam Line Break(MSLB) has been numerically investigated with direct vessel injections and an increased volume flow rate in some cold legs. Using FLUENT code, temperature distributions of the fluid in the downcomer and of reactor vessel including the core region have been calculated, together with the distribution of convective heat transfer coefficient(CHTC) at the cladding surface of the reactor vessel. The result shows that some parts of the core region of the reactor vessel have higher temperature gradient expressing higher thermal stress.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.49 no.4
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• pp.233-239
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• 2000

There are many difficult problems in analyzing the gas flow in puffer type circuit breaker such as complex geometry, moving boundary, shock wave and so on. To predict the interruption performance accurately, these should be considered in the simulation. In this paper, the analysis procedure of the cold gas flow in the circuit breaker is presented. Euler equation is solved by FVFLIC method which is an explicit time difference scheme for an unsteady flow computation. Moving boundaries are treated with a cell elimination-addition technique. The pressure and density in front of piston are calculated from the rate of the cell volume change. The presented method is applied to the real circuit breaker model and the pressure in front of the piston is good agreement with the experimental one.

• Journal of the Korean Society of Propulsion Engineers
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• v.3 no.3
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• pp.53-61
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• 1999

Impinging jets are observed when exhaust gases from missiles or V/STOL aircrafts impinge on the ground, flame deflector, ship deck, etc. The flow shows different patterns according to the nozzle geometry, nozzle-to-plate distance, and plate angle, for example. This paper describes experimental works on the phenomena (pressure distribution, occurrence of stagnation bubble, and so on.) when underexpanded supersonic jets impinge on a perpendicular flat plate using a supersonic cold-flow system, and compares the results with those obtained using a shock tunnel. The flow characteristics for the supersonic cold-flow system were also investigated. Surface pressure distribution of supersonic cold-flow system differed from that of shock tunnel because of water and temperature in the low-pressure chamber. Surface pressure distribution as to underexpanded ratio showed similar patterns together.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2004.08a
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• pp.207-215
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• 2004

There are various characteristics called for in work roll and intermediate rolls for cold rolling mills. Among these characteristics, the two main requirements are to ensure the quality of the rolled products and to reduce roll cost. To achieve these needs, resistance to wear, to thermal shock and to contact fatigue are especially important. This paper describes that new material(named DSR1) for intermediate rolls which greatly increases rolling campaign and improves resistance to wear has been developed. DSR1 was successfully manufactured and has been used in the cold rolling mill. It showed that Trial product was homogenous in hardness distribution and sufficient usable diameter. Also in service test, trial product is much more excellent rolling performance than conventional $5\%Cr$.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2001.04a
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• pp.23-23
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• 2001

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.169.1-169
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• 1996

No Abstract, See Full Text

• Bulletin of the Korean Space Science Society
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• 2011.04a
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• pp.29.2-29.2
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• 2011

Earth's bow shock is a transition layer across which properties of plasmas change irreversibly. Although some features of the bow shock are well described by continuities of fluxes of various macroscopic quantities, particle dynamics across the transition layer is very complicated. Observed phase space distributions show multiple ion beams and partially thermalized ions around the transition layer. In some cases, both hot magnetosheath ions and cold solar wind ions simultaneously exist in the magnetosheath. Electrons around the transition layer usually have flat-top distributions with temperature anisotropy. From the observed properties of the phase space distributions we will discuss thermalization processes that occur across the shock transition.

• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1353-1360
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• 2007

Three csp-like genes were identified in the Corynebacterium glutamicum genome and designated cspA, cspB, and cspA2. The genes cspA and cspA2 encode proteins, comprising of 67 amino acid residues, respectively. They share 83% identity with each other. Identity of those proteins with Escherichia coli Csp proteins was near 50%. The cspB gene encodes a protein composed of 127 amino acids, which has 40% and 35% sequence identity with CspA and CspA2, respectively, especially at its N-terminal region. Analysis of the gene expression profiles was done using transcriptional cat fusion, which identified not only active expression of the three genes at the physiological growth temperature of $30^{\circ}C$ but also growth phase-dependent expression with the highest activity at late log phase. The promoters of cspA and cspA2 were more active than that of cspB. The expression of the two genes increased by 30% after a temperature downshift to $15^{\circ}C$, and such stimulation was more evident in the late growth phase. In addition, the cspA gene appeared to show DNA-binding activity in vivo, and the activity increased at lower temperatures. Interestingly, the presence of cspA in multicopy hindered the growth of the host C. glutamicum cells at $20^{\circ}C$, but not at $30^{\circ}C$. Altogether, these data suggest that cspA, cspB, and cspA2 perform functions related to cold shock as well as normal cellular physiology. Moreover, CspA and its ortholog CspA2 may perform additional functions as a transcriptional regulator.

• Journal of the Korean Society of Propulsion Engineers
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• v.8 no.1
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• pp.8-15
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• 2004

Supersonic jets impinging on $60^{\cire}$ wedge were investigated to obtain fundamental design data for jet deflector It was of interest to study flow phenomena such as shock interaction and separation induced by shear layer. Experiments using supersonic cold flow system were conducted for Schlieren flow visualization and measurement of surface pressure. Numerical results were compared with the experimental results. The major parameters are underexpansion ratio, distance from nozzle to apex and design Mach number. Flow conditions were obtained for the wedge shock to attach on or detach from the wedge. The dominant feature of flow-field is shock pattern induced by the Interaction between the wedge shock and the barrel shock.