• BMB Reports
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• v.40 no.3
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• pp.325-332
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• 2007

The mitogen-activated protein kinase (MAPK) cascade is one of the major and evolutionally conserved signaling pathways and plays pivotal role in the regulation of stress and developmental signals in plants. Here, a novel gene, termed Gossypium hirsutum MAPK (GhMAPK), was isolated from cotton. The full-length cDNA of GhMAPK encodes for a 372 amino acid protein that contains all 11 of the MAPK conserved subdomains and the phosphorylationactivation motif, TEY. Amino acid sequence alignment revealed that GhMAPK shared high identity with group-C MAPK in plants and showed 83~89% similarities with MAPKs from Arabidopsis, apricot, pea, petunia, and tobacco. Southern blot analysis indicated that the GhMAPK belonged to a multygene family in cotton. Two introns were found within the region of genomic sequence. Northern blot analysis revealed that the transcripts of GhMAPK accumulated markedly when the cotton seedlings were subjected to various abiotic stimuli such as wounding, cold (4$^{\circ}C$), or salinity stress; Furthermore, GhMAPK was upregulated by the exogenous signaling molecules, such as salicylic acid (SA) and hydrogen peroxide ($H_2O_2C$), as well as pathogen attacks. These results indicate that the GhMAPK, which has a high degree of identity with group-C plant MAPKs, may also play an important role in response to environmental stresses.

• Journal of the Korean Geophysical Society
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• v.9 no.4
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• pp.397-408
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• 2006

Due to the temporal and spatial variability of the warming at and near the Antarctic Peninsular, it is required to better understand local climate at the issued region. The purpose of the study are to characterize surface radiation, air temperature and wind direction and investigate their relations at the King Sejong Station near the Antarctic Peninsular during last three and half years. While the study site was a weak radiative energy sink (positive net radiation) with annual mean of 15-20 Wm-2, it played a role as a strong sink in summer (December to January) with mean of 85 Wm-2, a magnitude that was significantly larger than those at other surface covered with snow or ice in Antarctica. Monthly averaged air temperature ranged from -7.7-2.8oC and the variations of monthly averaged air temperature showed the distinct differences with year. Northwesterly, westerly and easterly were dominant and the variability of air temperature could be explained by the variability of the frequency of wind direction with cold easterly and warm northwesterly/northerly to some degree, which in turn influenced radiation budget through albedo in summer.

• Journal of Energy Engineering
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• v.12 no.2
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• pp.84-92
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• 2003

A one-dimensional thermal-hydraulic analysis computer program is developed for thermal sizing of a copper bonded steam generator. It is assumed that the conduction heat transfer of copper region between the hot side and the cold side tube is one-dimensional and its thermal resistance is derived as a function of a tube pitch. The flow regions of the water/steam side are divided into four regions: subcooled, saturated, film boiling, and super-heated. The number of tube selected ranges from 250 to 3500 and the pitch to tube diameter (P/D) ratios are 1.4, 1.6 and 1.8 for the parametric study calculation. The calculation results showed that when the number of tube was 2500, the length of the heating tube was about 12 m and the outside diameter of the steam generator was about 3 m. If the P/D ratio increases, the thermal resistance of copper component also increases, however the length of the heating tube is not so much increased.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.14 no.2
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• pp.113-116
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• 1978

To ascertain the feasibility of the energy utilization in the sea adjacent to Korea, the distribution of the vertical temperature difference and the seasonal variation in the southeastern Yellow Sea are studied in relation to the sea water circulation. In summer, a region of high vertical temperature difference of approximately 16$^{\circ}C$ was found at a distance of approximately 40 miles from the western coast of Korea. It is located at the west of 125${\circ}$ 30`E and at the north of 34${\circ}$N. The vertical temperature structure is sustained by the inflow of Yellow Sea Warm Current water, the warming of the surface water of the Yellow Sea and the periodical renewal of the Yellow Sea Cold Water. It may be stated that power can be obtained from the sea water by making the use of the temperature difference. The vertical temperature difference was around 14$^{\circ}C$ in the western and southern waters of Jejudo Island. The vertical temperature difference decreases in autumn, and disappears due chiefly to the vigorous convective vertical mixing in winter when the northwest monsoon prevails. The power can be obtained from sea throughout the year, if power generation by the temperature difference is combined with that by wind and wave, and systemized in such a way that the former is employed in the hot season of summer, while the latter in winter and spring.

• BMB Reports
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• v.41 no.10
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• pp.693-698
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• 2008

The full-length cDNA of LebZIP2 (Lycopersicon esculentum bZIP2) encodes a protein of 164 amino acids and contains a N-terminal basic-region leucine zipper domain. Analysis of the deduced tomato LebZIP2 amino acid sequence revealed that it shares 85% sequence identity with both tobacco bZIP and pepper CcbZIP. LebZIP2 mRNA is expressed at a high level exclusively in flowers. Presently, LebZIP2 was strongly increased also following NaCl and mannitol treatments. No significant LebZIP2 expression was evident following cold treatment. Transient LebZIP2 overexpression resulted in increased NbNOA1 and NbNR transcript levels in Nicotiana benthamiana leaves. Our results indicate that LebZIP2 might play roles as an abiotic stress-signaling pathway and as a transcriptional regulator of the NbNOA1 or NbNR genes.

• Atmosphere
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• v.18 no.4
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• pp.299-316
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• 2008

The intensive upper-air observation network was organized over southwestern region of the Korean Peninsula during the Korea Enhanced Observing Program in 2005 (KEOP-2005). In order to examine the effect of additional upper-air observation on the numerical weather forecasting, three Observing System Experiments (OSEs) using Korea Local Analysis and Prediction System (KLAPS) and Weather Research and Forecasting (WRF) model with KEOP-2005 data are conducted. Cold start case with KEOP-2005 data presents a remarkable predictability difference with only conventional observation data in the downstream and along the Changma front area. The sensitivity of the predictability tends to decrease under the stable atmosphere. Our results indicates that the effect of intensive observation plays a role in the forecasting of the sensitive area in the numerical model, especially under the unstable atmospheric conditions. When the intensive upper-air observation data (KEOP-2005 data) are included in the OSEs, the predictability of precipitation is partially improved. Especially, when KEOP-2005 data are assimilated at 6-hour interval, the predictability on the heavy rainfall showing higher Critical Success Index (CSI) is highly improved. Therefore it is found that KEOP-2005 data play an important role in improving the position and intensity of the simulated precipitation system.

• Ocean and Polar Research
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• v.27 no.2
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• pp.205-214
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• 2005

From ancient Antarctic glacier ice, we extracted total genomic DNA that was suitable for prokaryotic 16S rDNA gene cloning and sequencing, and bacterial artificial chromosome (BAC) library and end-sequencing. The ice samples were from the Dry Valley region. Age dating by $^{40}Ar/^{39}Ar$ analysis on the volcanic ashes deposited in situ indicated the ice samples are minimum 100,000-300,000 yr (sample DLE) and 8 million years (sample EME) old. Further assay proved the ice survived freeze-thaw cycles or other re-working processes. EME, which was from a small lobe of the basal Taylor glacier, is the oldest known ice on Earth. Microorganisms, preserved frozen in glacier ice and isolated from the rest of the world over a geological time scale, can provide valuable data or insight for the diversity, distribution, survival strategy, and evolutionary relationships to the extant relatives. From the 16S gene cloning study, we detected no PCR amplicons with Archaea-specific primers, however we found many phylotypes belonging to Bacteria divisions, such as Actinobacteria, Acidobacteria, Proteobacteria $({\alpha},\;{\beta},\;and\;{\gamma})$, Firmicutes, and Cytophaga-Flavobacterium-Bacteroid$. BAC cloning and sequencing revealed protein codings highly identical to phenylacetic acid degradation protein paaA, chromosome segregation ATPases, or cold shock protein B of present day bacteria. Throughput sequencing of the BAC clones is underway. Viable and culturable cells were recovered from the DLE sample, and characterized by their 16S rDNA sequences. Further investigation on the survivorship and functional genes from the past should help unveil the evolution of life on Earth, or elsewhere, if any.

• Journal of the Korea Concrete Institute
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• v.21 no.5
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• pp.611-617
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• 2009

This paper reports an experimental study on the damage mechanism and resistance of Type I portland cement mortar and paste samples exposed to 5% sodium sulfate solution with different solution temperatures; namely, $4^{\circ}C$, $10^{\circ}C$ and $20^{\circ}C$. The resistance of mortar samples was evaluated using expansion, compressive strength and flexural strength measurements. Some microstructural observations such as x-ray diffraction, differential scanning calorimetry and scanning electron microscopy were also introduced to elucidate reactants formed by sulfate attack, especially in a low temperature condition. From the results, it was found that the degree of damage in the mortar samples was significantly associated with the temperature of sulfate solution. Low temperature of the sulfate solution led to the formation of thaumasite in mortar and paste samples, and subsequently a poor resistance to sulfate attack. Thus, it is noted that when concrete structures are exposed to sulfate media in the condition of a cold region or whether, special care should be taken.

• Archives of Plastic Surgery
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• v.42 no.3
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• pp.295-301
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• 2015

Background Glomus tumors were first described by Wood in 1812 as painful subcutaneous tubercles. It is an uncommon benign neoplasm involving the glomus body, an apparatus that involves in thermoregulation of cutaneous microvasculature. Glomus tumor constitutes 1%-5% of all hand tumors. It usually occurs at the subungual region and more commonly in aged women. Its classical clinical triad consists of pain, tenderness and temperature intolerance, especially cold sensitivity. This study reviews 15 cases of glomus tumor which were analyzed according to its anatomic location, surgical approach and histologic findings. Methods Fifteen patients with subungual glomus tumors of the hand operated on between January 2006 and March 2013, were retrospectively reviewed. Patients were evaluated preoperatively with standard physical examination including ice cube test and Love's test. Diagnostic imaging consisted of ultrasonography, computed tomography, and magnetic resonance imaging. All procedures were performed with tourniquet control under local anesthesia. Eleven patients underwent excision using the transungual approach, 3 patients using the volar approach and 1 patient using the lateral subperiosteal approach. Results Total of 15 cases were reviewed. 11 tumors were located in the nail bed, 3 in the volar pulp and 1 in the radial aspect of the finger tip. After complete excision, patients remained asymptomatic in the immediate postoperative period. In the long term follow up, patients exhibited excellent cosmetic results with no recurrence. Conclusions Accurate diagnosis should be made by physical, radiologic and pathologic examinations. Preoperative localization and complete extirpation is essential in preventing recurrence and subsequent nail deformity.

• Transactions of Materials Processing
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• v.29 no.4
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• pp.194-202
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• 2020

In this paper, experimental and numerical study on a combined sheet metal forming and plate forging of a seal part of a passenger car's hub bearing is conducted to develop the new process of which target is to remove machining process by plate forging and to achieve near-net shape manufacturing. The previous process of a sheet metal forming inevitably needed a machining process for making stepped sheet after conventional sheet metal forming in a progressive way. The stepped sheet is intended to be formed by plate forging in this study. Through the systematic way of developing the combined forming process using solid elements based-elastoplastic finite element method (FEM), several conceptual designs are made and an optimized process design in terms of geometric dimensioning and tolerance of straightness of the thin part is found, which is exposed to bending in metal forming of axisymmetric part. The predicted straightness measured by the slope angle of the tilted thin region is compared with the experimental straightness, showing that they are in a good agreement with each other. Through this study, a systematic approach to optimal process design, based on elastoplastic FEM with solid elements, is established, which will contribute to innovating the conventional small-scaled sheet metal forming processes which can be dealt with by solid elements.