• Title/Summary/Keyword: Cold and Heat

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Responses of Bacteria to TNT: Cells′Survival, SDS-PAGE and 2-D Electrophoretic Analyses of Stress-Induced Proteins (TNT에 대한 세균의 반응기작: 생존율, 스트레스 유도단백질의 SDS-PAGE 및 2-D 전기영동 분석)

  • 오계헌;장효원;강형일;김승일
    • Korean Journal of Microbiology
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    • v.38 no.2
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    • pp.67-73
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    • 2002
  • The cellular responses of soil-borne bacterium, Pseudomonas sp. HK-6 to explosive 2,4,6-trinitrotoluene (TNT) were examined. Two stress shock proteins (SSPs), approximately 70-kDa DnaK and a 60-kDa GroEL were found in HK-6 cells in response to TNT. Analyses of SDS-PAGE and Western blot using anti-DnaK and GroEL revealed that SSPs were induced in HK-6 cells exposed to 0.5 M of TNT far 6-12 hrs. The maximum induction of proteins was achieved at 8-hr incubation point after HK-6 cells'exposure to TNT. Similar SSPs were found to be induced in HK-6 cells by heat shock (shift of temperature, from $30^{\circ}C$ to $42^{\circ}C$) or cold shock (shift of temperature,$30^{\circ}C$ to $4^{\circ}C$).2D-PAGE of soluble protein tractions from the culture of Pseudomonas sp. HX-6 exposed to TNT demonstrated that approximately 450 spots were observed on the silver stained gels ranging from pH 3 to pH 10. Among them, 12 spots significantly induced and expressed in response to TNT were selected and analyzed. Approximately 60-kDa protein, which was assumed highly expressed on the gel, was used for amino acid sequencing. N-terminal microsequencing with in-gel digestion showed that N-terminal sequence of the TNT-induced protein, <$^1XXAKDVKFGDSARKKML^17$, shared extensive similarity with $^1XXAKDVKFGDSARKKML^17$, N-terminal sequence of (P48216) GroEL of Pseudomonas putida.

Ecological Green Roofs in Germany

  • Kohler, Manfred
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.4
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    • pp.8-16
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    • 2004
  • The industrialization of central Europe more than 100 ago marked the beginning of densely concentrated buildings in quickly growing cities. A cheap type of roofing material of that time was tar. But it was dangerous because it was high inflammable. Then some roofer had a splendid idea. They used sandy material as a final layer atop the impermeable tar layer. These roofs were much more fire resistant than the typical roofs. In this sandy layer some plant species began to grow spontaneously. This was the beginning of the green roof history of modern Europe. A number of these green roofs survived both world wars. In the early 80's in Berlin alone, 50 such buildings existed and they continued to be waterproof until the present day. Since the 1992 Earth Summit of 1992 in Rio de Janeiro(http://www.johannesburgsummit.org/html/basic_info/unced.html) the term "sustainable development" became of central interest of urban designers. In city regions green roofs had become synonymous with this term. With a small investment, long-lasting roofs can be created. Further back in history, more exciting examples of green roofs can be found. The hanging gardens of antiquity are well-known. There are also green roofs built as insulation against cold and heat all over the world. For over 20 years, roof greening in central Europe has been closely examined for various reasons. Roof greening touches several different disciplines. Of primary interest is the durability of the roofs. But ecologists are also interested in green roofs, for instance in biodiversity research. The beneficial effect of greening on water proofing was also proven. For some time, the issue of fire protection was investigated. According to tests, green roofs received a harsh careful rating. Their fire protective property is considered similar to that of tile roofs. Another recent impulse for the green roof movement in Germany has come from the evident improvement of storm water retention and the reduced burden on the sewer system. The question of whether and how much energy green roofs can save has become an urgent question. The state of the research and also various open questions from a central European point of view will be discussed in the context of international collaboration. Apart from academic considerations, those who involve themselves in this issue take a predominantly positive view of the numerous existing green roofs in Germany. In some cities, green roofs are the typical construction technique for new buildings. A few outstanding examples will conclude this review. In Germany, about 20 companies, some of which operate internationally, specialize in green roof consulting. Learning from each other in an open-ended way with respect to different construction techniques and applications in various climatic regions can only be accomplished through such international collaboration as is taking place here.

Production of High-Resolution Long-Term Regional Ocean Reanalysis Data and Diagnosis of Ocean Climate Change in the Northwest Pacific (북서태평양 장기 고해상도 지역해양 재분석 자료 생산 및 해양기후변화 진단)

  • Young Ho Kim
    • Journal of the Korean earth science society
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    • v.45 no.3
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    • pp.192-202
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    • 2024
  • Ocean reanalysis data are extensively used in ocean circulation and climate research by integrating observational data with numerical models. This approach overcomes the spatial and temporal limitations of observational data and provides high-resolution gridded information that considers the physical interactions between ocean variables. In this study, I extended the previously produced 12-year (2011-2022) Northwest Pacific regional ocean reanalysis data to create a long-term reanalysis dataset (K-ORA22E) with a horizontal resolution of 1/24° spanning 30 years (1993-2022). These data were analyzed to diagnose long-term ocean climate change in the Korean marginal seas. Analysis of the K-ORA22E data revealed that the axis of the Kuroshio extension has shifted northward by approximately 6 km per year over the past 30 years, with a significant increase in sea surface temperature north of the Kuroshio axis. Among the waters surrounding the Korean Peninsula, the East Sea exhibited the most significant temperature increase. In the East Sea, the temperature increase was more pronounced in the middle layer than in the surface layer, with the East Korea Warm Current showing a rate two to three times higher than the global average. In the central Yellow Sea, where the Yellow Sea Bottom Cold Water appears, temperatures increased over the long-term, but decreased along the west and south coasts of the Korean Peninsula. These spatial differences in long-term temperature changes appear to be closely related to the heat transport pathways of warm water from the Kuroshio Current. High-resolution regional ocean reanalysis data, such as the K-ORA22E produced in this study, are essential foundational data for understanding long-term variability in the Korean marginal seas and analyzing the impacts of climate change.

The Clinical Study of Biyun(sinusitis) in Children (소아비연(小兒鼻淵)에 대(對)한 임상적(臨床的) 연구(硏究))

  • Park Eun-Jeong;Lee Hae-Ja
    • The Journal of Pediatrics of Korean Medicine
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    • v.12 no.1
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    • pp.111-131
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    • 1998
  • 1. In oriental medicine, the case of sinusitis can be explained, not only external invasion such as PUNG HAN(wind-cold), PUNG YUL(wind-hot), SHUP YUL(damp-heat), but also functional disorder of internal organ such as spleen(脾), lung(肺), kidney(腎) The western medicine classified the cause of sinusitis as two factors. That is bacterial infection factor-Haemophilus influenza, streptococcus pyogeues, streptococcus pneumonia- and viral infection factor-Rhinovirus, parainfluenza, Echo28, Coxsacki21, Sinusitis is complicated to allergic rhinitis, chronic otitis media purulent, chronic tonsilitis, sinubronchitis. On the condition of nasal septum deformity, turbinates deformity, nasal septum deviation, sinusitis can be developed. the predisposing factors of sinusitis is swimming, air pollution, malnutrition, shortage of immunity.2. According to survey, sinusitis occurred that children from 4 to 12 years old and from 5 to 7 years old occupied 70% 3. From the past history data, they experienced chronic tonsillar hypertropy(20%), otitis media, atopic dermatitis, allergic rhinitis, bronchial asthma, pneumonia, bronchiolitis, chronic sore throat, urticaria, milk allergy in sequence. 4. the symptoms of sinusitis is nasal obstruction, postnasal dripping, purulunt(yellow)or white discharge, cough, nose bleeding in sequence. nasal obstruction take the portion of 95%, postnasal dripping 65%, night time or early morning cough 60%. 5. The suffering period of sinusitis is 6 month minimum, 4 years maximum, most cases are included in a year. The suffering period of children was shorter than adult. 6. Diagnosis depend on inspection of nasal cavity, postnasal dripping, X-ray finding. 75% of patient(15case) showed both maxillary sinusitis, 25%(5cases) showed left or right maxillary sinusitis. 7. Treatment of oriental medicine, consist of Herb-medicine, acupuncture and exposing of Lazer beam. Kamihyunggyeyungyotang(加味荊芥蓮翹湯) is administered mainly as the medical therapy, Kamigwaghyangjeungkisan(加味藿香正氣散) Kimizwakwieum(加味左歸飮), Kamihyangsosan(加味香蘇散) is administered for a additional symptoms which occurred by influenza recurrence. Kamijeonxibackchulsan(加味錢氏白朮散) is administered to treat gastro-intestine trouble patients who have sinusitis. 8. The period of treatment is varied with patient conditions and X-ray finding. The minimal period is 35days, maximal period is 202days. So it took about 86days in average and about 50% of patient(10cases) is recovered in one or two month.

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Evaluation of Mechanical Performance Considering Prolonged Length of Glass Fiber-Reinforced Composite on Structure Weakness by Thermal Stress at Secondary Barrier in Cryogenic Liquified Gas Storage (극저온 액화가스 화물창 2차방벽 구조 열 응력 취약 부 Prolonged 길이 고려 유리섬유 강화 복합재 기계적 물성 평가)

  • Yeon-Jae Jeong;Hee-Tae Kim;Jeong-Dae Kim;Jeong-Hyun Kim;Seul-Kee Kim;Jae-Myung Lee
    • Composites Research
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    • v.36 no.4
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    • pp.246-252
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    • 2023
  • A secondary barrier made of glass fiber reinforced composites has been installed infinitely using automatic bonding machine(ABM) in membrane type LNG cargo containment system (CCS). At the same time, significant thermal stress due to cryogenic heat shrinkage has occurred in the composite on the non-bonding area between the adhesive fixation at both ends. There have been studies from the perspective of structural safety evaluation taking this into account, but none that have analyzed mechanical property taking an prolonged length into account. In this study, 2-parameter Weibull distribution statistical analysis was used to standardize reliable mechanical property for actual length, taking into account the composite's brittle fracture of ceramic material with wide fracture strength dispersion. Related experimental data were obtained by performing uniaxial tensile tests at specific temperatures below cryogenic condition considering LNG environment. As a result, the mechanical strength increased about 1.5 times compared to -20℃ at -70℃ and initial non-linear behavior of fiber stretched was suppressed. As the temperature decreased until the cryogenic, the mechanical strength continued to increase due to cold brittleness. The suggested mechanical property in this study would be employed to secure reliable analysis support material property when assessing the safety of secondary barrier's structures.

Evaluation of Thermal Insulation Properties of Covering Materials to Protect Peach Trunks against Freezing Injury (복숭아 주간부 동해 예방을 위한 피복재의 보온성 평가)

  • Shin, Hyunsuk;Yun, Seok Kyu;Choi, In Myung;Kim, Sung Jong;Yun, Ik Koo;Nam, Eun Young;Kwon, Jung Hyun
    • Journal of Bio-Environment Control
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    • v.25 no.4
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    • pp.288-293
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    • 2016
  • The study was performed to evaluate thermal insulation covering materials (TICMs) to protect peach trunks against freezing temperatures in winter season by investigating thermo-physical properties and practical thermal insulation effect of the TICMs which was made of white non-woven fabrics, yellow paper sheets, and waterproof fabric pads. Among the three TICMs, Waterproof fabric pad (double layer) possessed the best performance about thermal insulation rate and thermal resistance among three kinds of TICMs. Day thermal insulation effects of waterproof fabric pad, which prevent from temperature rise on the bark tissues of trunks during the day time, were $14.09^{\circ}C$. Night thermal insulation effects of them, which prevent from temperature decline on the bark tissues of trunks at night time, were $7.23^{\circ}C$. Waterproof fabric pad showed the highest day and night thermal insulation effects. Thus our results suggest that development of TICMs using waterproof fabric pad might be helpful to protect the bark tissues of trunks from freezing injury.

A study on characteristics for a resistive SFCL with gold layer (Gold층을 가진 저항형 초전도 한류기에 대한 특성연구)

  • Choi, Hyo-Sang;Hyun, Ok-Bae;Kim, Hye-Rim;Hwang, Si-Dole;Kim, Sang-Joon
    • 한국초전도학회:학술대회논문집
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    • v.9
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    • pp.348-351
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    • 1999
  • We investigated current limiting properties for an SFCL of YBCO thin film coated with an Au layer. The YBCO film of 1 mm wide and 400 nm thick could carry the current 9.6 A$_{peak}$ without quench. The SFCL limited the fault current below 7.6 A$_{peak}$, which otherwise increases above 65 A$_{peak}$ and melted down at the potential fault current of about 100 A$_{peak}$ which is 10 times greater than the quench current. This means that the Au layer successfully protected the superconducting film by dispersing the heat generated at hot spots and electrically shunting the YBCO film.

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Design Criteria Derivation of Supercritical Carbon Dioxide Power Cycle based on Levelized Cost of Electricity(LCOE) (전력단가추정기반 초임계 이산화탄소 발전 시스템 최적 설계 인자 도출)

  • Park, Sungho;Cha, Jaemin;Kim, Joonyoung;Shin, Junguk;Yeom, Choongsub
    • Clean Technology
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    • v.23 no.4
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    • pp.441-447
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    • 2017
  • The economic analysis for the power plant developed in the conceptual design phase is becoming more important and, research on process optimization for process development that meets the target economic is actively carried out. In the filed of power generation systems, economic assessment methods to predict the levelized cost of electricity (LCOE) has been widely applied for comparing economic effect quantitatively. In this paper, the platform that design criteria of key component required to optimize economic of power cycle can be calculated reversely was established roughly and design criteria of the key equipment (Compressor, turbine, heat exchanger) required to meet the target LCOE (the LCOE of supercritical steam Rankine cycle) was derived when the supercritical $CO_2$ power cycle is applied to the coal-fired power plant.

An Experimental Study on Flow Distributor Performance with Single-Train Passive Safety System of SMART-ITL (SMART-ITL 1 계열 피동안전계통을 이용한 유동분사기 성능에 대한 실험연구)

  • Ryu, Sung Uk;Bae, Hwang;Yang, Jin Hwa;Jeon, Byong Guk;Yun, Eun Koo;Kim, Jaemin;Bang, Yoon Gon;Kim, Myung Joon;Yi, Sung-Jae;Park, Hyun-Sik
    • Journal of Energy Engineering
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    • v.25 no.4
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    • pp.124-132
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    • 2016
  • In order to estimate the effect of flow distributors connected to an upper nozzle of CMT(Core Makeup Tank) on the thermal-hydraulic characteristics in the tank, a simplified 2 inch Small Break Loss of Coolant Accident(SBLOCA) was simulated by skipping the decay power and Passive Residual Heat Removal System(PRHRS) actuation. The CMT is a part of safety injection systems in the SMART (System Integrated Modular Advanced Reactor). Each test was performed with reliable boundary conditions. It means that the pressure distribution is provided with repeatable and reproducible behavior during SBLOCA simulations. The maximum flow rates were achieved at around 350 seconds after the initial opening of the isolation valve installed in CMT. After a short period of decreased flow rate, it attained a steady injection flow rate after about 1,250 seconds. This unstable injection period of the CMT coolant is due to the condensation of steam injected into the upper part of CMT. The steady injection flow rate was about 8.4% higher with B-type distributor than that with A-type distributor. The gravity injection during hot condition tests were in good agreement with that during cold condition tests except for the early stages.

A Study on the Characteristics of Descriptions of the Perspiration in "Hwangjenaegyeong(黃帝內經)" (황제내경(黃帝內經)에 보이는 한(汗)관련 서술(敍述)의 특징(特徵)에 대한 고찰(考察))

  • Lyu, Jeong-Ah;Jang, Woo-Chang;Baik, You-Sang;Jeong, Chang-Hyun
    • Journal of Korean Medical classics
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    • v.23 no.2
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    • pp.205-223
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    • 2010
  • In Korean Traditional Medicine(abbreviated to K.T.M.), hyperhidrosis and anhidrosis are the targets of the medical treatment. Furthermore sweating appearance is also one of the important symptoms which explain a particular situation of the patient in K.T.M. And at "Sanghanron(傷寒論)" which is a traditional chief clinical bible written by Jang Gi(張機) later Han dynasty(漢代) in China made full use of the various kinds of diaphoresis[汗法] as a main medical treatment with purgation therapy[下法] and emetic therapy[吐法]. So the sweat in itself not only is the disease, but also is one of the symptoms explain a disease pattern. This thesis inquires into "Hwangjenaegyeong(黃帝內經)" referring to sweat which is the origin of recognition to the sweat in K.T.M. Some theses similar to this research had been made progresses and already reported, but most of them have classified the contents into biology, pathology, diagnosis, treatment after the model of western medical theory. In the aspect of comparative studying with other literature and clinic practical using, we found characteristics of referring to sweat in "Hwangjenaegyeong(黃帝內經)". And we classify the characteristics into some categories as follows. 1. There are some terms which make a title including sweat and symbolize the characteristics, for example sweat of soul[魄汗], sweat of death[絶汗], sweat of streaming[灌汗], sweat of weakness[白汗], sweat of sleep[寢汗], sweat of bright and heat[炅汗], sweat of kidney[腎汗], sweat of escaping[漉汗], cold sweat[寒汗], sweat on the head[頭汗], hyperhidrosis[多汗], heavy sweat[大汗]. But there aren't spontaneous sweat[自汗] or sweat like a thief[盜汗] which are the normal terms referring to sweat in history of K.T.M. And there are several descriptions about sweat appearance such as sweating in half of body[汗出偏沮], sweating in the rear end and thigh and knee[汗出尻陰股膝], hyperhidrosis in the neck and aversion to wind[頸多汗惡風], hyperhidrosis in the head and face and aversion to wind[頭面多汗惡風], cannot stopping the sweating under head[頭以下汗出不可止], make a person sweat to one's feet[令汗出至足], sweating like escaping[漯漯然汗出], sweating like soaking[汗出如浴], sweating become moist[汗出溱溱], hardly escaping sweat[汗大泄], escaping sweating[漉漉之汗], sweat moisten the pores [汗濡玄府], ceaseless sweating like pouring[汗注不休] sweating like pouring and vexation[汗注煩心], damp with sweat[汗汗然], sweating spontaneously[汗且自出], removal of fever with sweat drying[熱去汗稀]. That can be divided into sweat region and sweat form. 2. There are detailed explanations of the principle of perspirations caused by hot weather, hot food, hard working and meeting damp pathogen. 3. There are some explanations of the principle of removing fever due to the excessive heat from internal and external body through sweating by replenishing the body fluid. And many descriptions about overcoming the febrile disease by dropping temperature through sweating and many diaphoresis for curing. 4. There are some descriptions about five Jang organs perspirations and attachment of five mucous body fluid to five Jang organs. 5. There are pathogenic progresses after sweating affected by the Six Atmospheric Influences and water. And detailed explanations of disease mechanism a sweat leading to another disease. 6. There are descriptions about various sweat absent situations.