• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.234-242
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• 2008

To identify low temperature-induced genes of wheat chromosome substitution line 5D, suppression subtractive hybridization (SSH) was performed with mRNAs from leaf samples that treated with low temperature ($4^{\circ}C$). A cDNA library was constructed using mRNA isolated from wheat chromosome substitution line 5D leaves treated with low temperature ($4^{\circ}C$). The nucleotide and deduced amino acid sequences of the putative gene products were compared. wfr-9 and wfr-32 showed identity over 90% related to vernalization gene. Other two genes, wfr-77 and wfr-83 which is related to freezing-resistant gene have also identity over 90%. This result suggest that those genes may be transcribed into antifreeze proteins which are accumulated within leaf apoplasts, when wheat chromosome substitution line 5D is acclimated during low temperature treatment.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.61-67
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• 2010

Six evergreen ground-cover species including Ajuga reptans, Carex morrowii 'Variegata', Dianthus 'Night Star', Hedera helix 'Ivalace', Pachysandra terminalis, and Sedum rupestre, were tested for cold acclimation with three different light treatments (0, 30, and 70% of shading, respectively) to select potential candidates for over-wintering in central Korea. Among those species, Ajuga reptans, Carex morrowii 'Variegata', and Sedum rupestre performed well, which had more than 50% of survival rate in all light conditions. However, Dianthus 'Night Star' had less than 40% under any light treatment and the other two species, Hedera helix 'Ivalace' and Pachysandra terminalis, survived more than 40% only with 70% of shading condition. These results imply that those candidate species having less cold hardness in central Korea could have the potential to could over-winter as long as a proper light condition is provided.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.5
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• pp.341-351
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• 2002

In order to examine the mechanistic basis for differential sensitivities to chilling and subsequent recovery between two rice (Oryza sativa L.) cutivars, a chilling-tolerant japonica type (Ilpumbyeo) and a chilling-susceptible indica type (Taebaekbyeo), changes of physiological responses and antioxidant enzymes were investigated. Both cultivars at 3 leaf stage were exposed at a low temperature of $5^{\circ}C$ for 3 days and subsequently recovered in a growth chamber at a $25^{\circ}C$ for 5 days with 250 mmol $m^{-2}$ $s^{-1}$. Physiological parameters such as leaf fresh weight, relative water content, cellular leakage, lipid peroxidation, and chlorophyll a fluorescence showed that the chilling tolerant cultivar had a high tolerance during chilling. However, the chilling-susceptible cultivar revealed severe chilling damages. The chilling-tolerant cultivar was also faster in recovery than the chilling-susceptible cultivar in all parameters examined. We analyzed the activity and isozyme profiles of four antioxidant enzymes which are: superoxide dismutase (SOD), caltalase (CAT), ascorbate peroxidase (APX), and glutation reductase (GR). We observed that chilling-tolerance was due to a result of the induced or higher antioxidant enzyme system, CAT and APX in leaves and SOD, CAT, APX, and GR in roots. Especially, we observed the most significant differences between the chilling-tolerant cultivar and -susceptible cultivar in CAT and APX activity. Also in isozyme profiles, CAT and APX band intensity in the chilling-tolerant cultivar was distinctively higher than in the chilling-susceptible cultivars during chilling and recovery. Thus, the cold stability of CAT and APX are expected to contribute to a tolerance mechanism of chilling in rice plants. In addition, the antioxidative enzymes activity in roots may be more important than in that of leaves to protect chilling damage on rice plants.

• Journal of agriculture & life science
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• v.45 no.2
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• pp.1-7
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• 2011

Three evergreen ground-cover species were tested and evaluated for cold acclimation with different lights in central Korea to enrich the varieties of ground cover plants for over-wintering. Nandina domestica which recently started to be planted in central Korea had high chlorophyll unit with shade condition in both locations, Banpo and Gwacheon during winter. Light condition had no influence on chlorophyll unit in Banpo after April, meanwhile full sun condition decreased chlorophyll unit significantly in Gwacheon, which is exposed to cold wind from north. Ophiopogon japonicas had more than double chlorophyll unit in shade condition compared to that in full light condition in both investigation areas in February. In addition, higher value of L and b in shade condition had been maintained than in full sun condition throughout investigation period. However, leaves in Gwacheon were de-colorized in full sun condition after over-wintering. Pachysandra terminalis had more higher chlorophyll unit in shade condition compared to in full sun condition in both investigation areas in February. But light conditions had not effect the difference of chlorophyll unit of Gwacheon in April. Therefore, The present results suggest that evergreen ground covers with shade condition would be used as landscape materials in the capital area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.3
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• pp.220-239
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• 2021

Grasses (Poaceae) belong to the biggest plant family among angiosperms and it cover around 20% of the earth's surface. The members of this family are mostly utilized as food resources by humans and animals but they are also valuable in terms of evolution and ecology. The member of the subfamily Pooideae represents, temperate grasses, and includes a number of economically important crops and belongs to the clade BOP (including the subfamilies Bambooideae, Oryzeae, and Pooideae). This subfamily is the largest among all grass families. The special features of this subfamily are cold acclimation and vernalization. The members of Pooideae subfamily with the aforementioned special features are thought to have evolved in the Cenozoic era when the temperature on earth started to cool down, which triggered the diversification of this subfamily through adaptation to cold weather. The agricultural origin of wheat, barley, oat, and rye is attributed to fertile crescent and thereafter they were domesticated through Neolithic evolution. The history of domestication of each Pooideae crop is distinct and is based on their purpose. Recently, breeding of these crops is performed differently due to the development of new technologies such as genomics and genome editing. This review article summarizes the evolutionary history of the members of the subfamily Pooideae and use of pre-existing information for future breeding efforts.

• Journal of Oriental Neuropsychiatry
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• v.4 no.1
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• pp.77-97
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• 1993

Human and animals are living by acclimation to environmental changes like high and cold temperature, nose, confinement, etc. If the above changes reach a defined levels, some physiological abnormal state could appear, which we call them as stress state. Catecholamines are excreted by the sympathetic-adrenomedullary system in free from in urine. Catecholamines are derived from the adrenal medulla and urinary epinephrine can be taken as a rough estemation of the activity of this gland. Many scientist reported the endocrinological change, excretion of catecholamine and its metabolites, stomach ulcer formation, etc. under the condition of the confinement and high temperature. In this study author gave restraint, electric shock and immersion stress to rats by administrating by HPLC and got the following results. 1. In the restriant experiment, epinephrine contents in control rat was 194.7 ng, but in Bohyulanshintang administered rat urine 198.9 ng of epinephrine was found. 2. In the electrical shock experiment, 199.5 ng of epinephrine was found in the control rat urine, but in Bohyulanshintang administered rat urine epinephrine content was 142.4 ng. 3. Dopamine contents in control rat urine the immersion environment was 118.9 ng, but in Bohyulanshintang administered rat urine only 55.2 ng of dopamine was found. 4. Incontrol rat stomach there appeared focal erosion and inflamatory exudate, but in experimental group these symptom were turned to mild condition.

• The Korean Journal of Zoology
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• v.35 no.3
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• pp.263-270
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• 1992

Responses of immunoreactive atrial natriuretic peptide (ir-ANP) to environmental temperature were studied in the freshwater catfish, Silums usotus, acclimated at various temperatures for one week. According to increase in environmental temperature, plasma sodium and chloride concentrations, and osmolality were significantly increased, while hematocrit showed a marked decrease. When the catfishes frere acclimated at cold $(3^{\circ}C)$ or warm $(18^{\circ}C)$ temperatures, ir-ANP contents in the atrium and bulbus arteriosus were significantly lower than those at $9^{\circ}C.$ However, ventricular contents of ir-ANP urere not different among the three groups.Levels of plasma ir-ANP in the catfish increased in response to the increment of acclimated temperature. On the basis of these results, we suggest that the environmental temperature may modulate synthesis and secretion of ir-hUP in the fish. We also suggest that changes of plasma ir-ANP levels may be associated with the control of body fluid homeostasis.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.697-702
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• 2005

The aim of this study was to divide vocalizations of laying hens (Hy-Line Brown) into general vocalizations (GVs), heat stress-related vocalization (HSV), and cold stress-related vocalizations (CSVs) and to determine if they are classified by the discriminant function analysis method. Thirty laying hens, 65-wk-old, were recorded using digital video recorders 2 times from 10:00 to 14:00 h in each thermal environment (thermoneutral: $22.0{\pm}1.8^{\circ}C$, too hot: $32.0{\pm}2.0^{\circ}C$, too cold: $8.0{\pm}1.9^{\circ}C)$ after a 7 day acclimation period. When the laying hens were not recorded, they were kept in thermoneutral conditions. The GVs, HSV, and CSVs were divided based on the shapes of spectrums and spectrograms. The GVs, HSV, and CSVs were identified as 5, 1, and 3 types, respectively. Pitch, intensity, duration, formant 1, formant 2, formant 3, and formant 4 among the thermal environment-related vocalizations were significantly different (P<0.001). The discrimination rate determined by discriminant function analysis was 86.2%. These results suggest that HSV and CSVs are present and may be used as an indicator of the thermal environment.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.78.1-78
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• 2003

An osmotin-like protein (CAOSMl) gene was isolated from pepper leaves infected with the avirulent strain Bv5-4a of Xmthomonas campestris pv. vesicatoria. The cDNA encodes a polypeptide of 250 amino acids with a molecular mass of 27, 361 Da. Its amino acid sequence is highly homologous to various osmotin-like proteins from other plant species. The CAOSMl gene expression was organ- and tissue-specifically regulated In pepper plants. The CAOSMl mRNA was intensely localized in the endodermis area of root tissue and in the phloem cells of vascular bundles of red fruit tissue, but not in leaf, stem, and green fruit tissues of healthy pepper plants. Infection by X. c. pv vesintoria, Colletotrichum coccodes, or Phytopkhora capsici iinduced CAOSMl transcription in the leaf or stem tissues. Expression of the CAOSMl gene was somewhat higher in the incompatible than the compatible interactions of pathogens with pepper. The CAOSMl mRNA was prevalently localized in the phloem cells of the vascular bundle of leaf tissues infected by C. coccodes. The CAOSMl gene was activated in leaf tissues by treatment with ethylene, methyl jasmonate, high salinity, cold acclimation and mechanical wounding, but not by abscisic acid (ABA) and drought. These results indicate that the pepper CAOSMl protein functions in response to Pathogens and some abiotic stresses in pepper plants

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.339-343
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• 2000

When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.