• Title/Summary/Keyword: Cold Shock

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Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena

  • Goo, In Bon;Im, Jae Hyun;Gil, Hyun Woo;Lim, Sang Gu;Park, In-Seok
    • Development and Reproduction
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    • v.19 no.3
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    • pp.127-134
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    • 2015
  • The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

Polyunsaturated Fatty Acids in Male Ruminant Reproduction - A Review

  • Tran, Len Van;Malla, Bilal Ahmad;Kumar, Sachin;Tyagi, Amrish Kumar
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.5
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    • pp.622-637
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    • 2017
  • Fatty acids such as n-3 and n-6 polyunsaturated fatty acids (PUFA) are critical nutrients, used to improve male reproductive performance through modification of fatty acid profile and maintenance of sperm membrane integrity, especially under cold shock or cryopreservation condition. Also, PUFA provide the precursors for prostaglandin synthesis and can modulate the expression patterns of many key enzymes involved in both prostaglandin and steroid metabolism. Many studies carried out on diets supplemented with PUFA have demonstrated their capability to sustain sperm motility, viability and fertility during chilling and freezing as well as improving testis development and spermatogenesis in a variety of livestock species. In addition to the type and quantity of dietary fatty acids, ways of addition of PUFA to diet or semen extender is very crucial as it has different effects on semen quality in male ruminants. Limitation of PUFA added to ruminant ration is due to biohydrogenation by rumen microorganisms, which causes conversion of unsaturated fatty acids to saturated fatty acids, leading to loss of PUFA quantity. Thus, many strategies for protecting PUFA from biohydrogenation in rumen have been developed over the years. This paper reviews four aspects of PUFA in light of previous research including rumen metabolism, biological roles, influence on reproduction, and strategies to use in male ruminants.

Degradation of the Parasporal Crystal Proteins of Bacillus thuringiensis and Induction of Stress Protein Synthesis in Bt $\delta$-endotoxin Ingested Larvae of Fall Webworm, Hyphantria cunea (Bacillus thuringiensis 의 내독소 단백질의 분해와 흰불나방 섭취유충에서 스트레스 단백질 합성의 유발)

  • 전향미;유용만;강석권;서숙재
    • Korean journal of applied entomology
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    • v.33 no.3
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    • pp.178-183
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    • 1994
  • The prolein components of each crystal toxin of Bacillus thuringiensis kurstaki and alzawai were separated by SDS-PAGE. The major clqistal proteins from both shins were composed of paiypeptides having molecular weights of 130 kd and 64 kd. Digestive mixture of both. toxins with t~ypsin and gut juices shared 62 kd polypeptide which may be major actwe toxh. However, aizawal produced much less amount of 62 kd polypeptide than kurstaki did. On ingestion with Bt &-endotoxin, larvae of Hyphantria cunea developed 45 kd stress protein in the several tissues including fat body, which was induced by heat and cold shock.

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Thermal Diary - 24 hours' Exposed Temperature and Thermal Comfort in Urban Life, Summer (여름철 도시의 일상생활에서 폭로되는 환경온도와 온냉감 평가)

  • 이민정;전정윤
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2002.11a
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    • pp.333-337
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    • 2002
  • All the outdoor and indoor spaces are connected with each other. The human being moves toward those spaces and experiences temperature fluctuation between the natural and artificial temperature. We conducted an experiment which subjects are the college students wearing the data logger in urban life, and measured 24 hours' exposed temperature and thermal comfort in summer. Results were as follows. 1. Most subjects get weather information(84.6%). Fashion(46.2%) and weather (30.8%) are the reasons to select clothes. They spend their time in indoor environment for 84.92% hours of a day and have an air-conditioner(61.5%) in their houses. 2. Exposed temperature fluctuation were from 33.8$^{\circ}C$ to 15.6$^{\circ}C$. The median value of experienced temperature were 26-27$^{\circ}C$ and average temperature was 26.3$^{\circ}C$. Subjects experienced cold shock of 3.96 times in a day and 67.21% of all evaluated thermal comfort in the range of -1 and 1 by ASHRAE 7 Category Scales. Artificial environment which connected with outside let people experienced temperature fluctuation in wide range.

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Simulation of Dynamic Characteristics of a Trigenerative Climate Control System Based On Peltier Thermoelectric Modules

  • Vasilyev, G.S.;Kuzichkin, O.R.;Surzhik, D.I.
    • International Journal of Computer Science & Network Security
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    • v.21 no.6
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    • pp.252-257
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    • 2021
  • The application of the principle of trigeneration allows to simultaneously provide electricity to power electronic devices, as well as heat and cold to create the necessary microclimate of the premises and increase efficiency compared to separate cooling and heating systems. The use of Peltier thermoelectric modules (TEM) as part of trigenerative systems allows for smooth and precise control of the temperature regime, high manufacturability and reliability due to the absence of moving parts, resistance to shock and vibration, and small weight and size parameters of the system. One of the promising areas of improvement of trigenerative systems is their modeling and optimization based on the automatic control theory. A block diagram and functional model of an energy-saving trigenerative climate control system based on Peltier modules are developed, and the transfer functions of an open and closed system are obtained. The simulation of the transient characteristics of the system with varying parameters of the components is performed. The directions for improving the quality of transients in the climate control system are determined, as well as the prospects of the proposed methodology for modeling and analyzing control systems operating in substantially nonlinear modes.

AFTL: An Efficient Adaptive Flash Translation Layer using Hot Data Identifier for NAND Flash Memory (AFTL: Hot Data 검출기를 이용한 적응형 플래시 전환 계층)

  • Yun, Hyun-Sik;Joo, Young-Do;Lee, Dong-Ho
    • Journal of KIISE:Computer Systems and Theory
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    • v.35 no.1
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    • pp.18-29
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    • 2008
  • NAND Flash memory has been growing popular storage device for the last years because of its low power consumption, fast access speed, shock resistance and light weight properties. However, it has the distinct characteristics such as erase-before-write architecture, asymmetric read/write/erase speed, and the limitation on the number of erasure per block. Due to these limitations, various Flash Translation Layers (FTLs) have been proposed to effectively use NAND flash memory. The systems that adopted the conventional FTL may result in severe performance degradation by the hot data which are frequently requested data for overwrite in the same logical address. In this paper, we propose a novel FTL algorithm called Adaptive Flash Translation Layer (AFTL) which uses sector mapping method for hot data and log-based block mapping method for cold data. Our system removes the redundant write operations and the erase operations by the separating hot data from cold data. Moreover, the read performance is enhanced according to sector translation that tends to use a few read operations. A series of experiments was organized to inspect the performance of the proposed method, and they show very impressive results.

Studies on Therapeutic range, Symptom, Pathology, and composition of Ginseng Radix -main blended Prescriptions from Donguibogam (동의보감(東醫寶鑑)에 수록(收錄)된 인삼(人蔘)이 주약(主藥)으로 배오(配伍)된 방제(方劑)의 활용(活用)범위, 병증, 주치(主治), 병리(病理) 및 구성내용(構成內容) 조사(調査))

  • Cho, Dae-Yeon;Jeong, Jong-Kil;Yun, Young-Gab
    • Herbal Formula Science
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    • v.9 no.1
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    • pp.35-82
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    • 2001
  • In the Encyclopedia Medica Koreana(Dongeuibogam), I have researched 245 prescriptions in which Panax Ginseng plays an important role. And I have got the following results. The healing scope and frequency of ginseng-mainly-included prescriptions are Child Part 29(11.83%), Violent Cough Part 23(9.38%), Sick-by-Cold Part 21(8.57%), Oncosis Part 16(6.53%), Overwork Part 14(5.71%), Gynecologic Part 14(5.71%), Internal Part 13(5.3%), Apoplexy Part 11(4.48%), Mind Part 10(4.08%) and Fecal Part 10(4.08%) prescriptions. And also each of Nausea Part, Anger Part, and Spirit parts has the same 5 (2.04%) prescriptions. And each of Qi Part, Diabetes Meatus Part, Malaria Part, and Humoral Part has 4(1.63%) prescriptions. And each of Foot Part, Choleraic Part, Genital Part, Blood Part, and Voice Part has 3 (1.2%). All of these prescriptions cover 88.88%. And besides listed parts above, Panax Ginseng is all used in 48 Parts: Body-Mind Part. Mouth-Tongue Part, Breast Part, Muscle Part, Swelling Part, Urine Part, Epidermis Part, Heat Part, Anus Part, Stomach Part, Eye Part, Laryngopharynx Part. Uterus Part" Heavy Stomach Part, Head Part, Pulse Part, Hair Part, Navel Part, Emetic Part, Costal Part, Edema Part, Vomiting Part, Superstitious Part, and Cardiac Part, etc. Of the prescriptions in which Panax Ginseng plays an important role, the most representative diseases, which more than 86.8% prescriptions cure, are shock, numbness from cold, Taeeum disease, oncosis, overwork, sick from eating, numbness of extremities, diarrhea, tachycardia, forgetfulness, nausea, heat from kidney, nocturnal emission, short breath, diabetes meatus, malaria, sweating, sweating overnight, beriberi, cholera, insomnia from enervation, sialitis, navel pain, hemorrhage, and loss of voice. The pathology of the prescriptions in which Panax Ginseng plays an important role is divided into the organ problems, six natural factors, seven extreme feelings, unbalanced humoral status, overwork, and, unbalance of qi and blood. Spleen, heart, and uterus is the main cause of organ problems; wind and cold are the main cause of six natural factors; heavy humors are the main cause of unbalanced humoral status; the stasis of seven feelings are the main cause of seven extreme feelings; the lack of stamina and overwork are the main cause of the overwork; the lack of qi, the lack of blood, and, the lack of qi and blood are the main cause of the unbalance of qi and blood. After I have researched the contents of the prescriptions in which Panax Ginseng plays an important role, I could understand the addition of the different prescriptions, combination of medicines, and the role of medicine groups associated with Panax Ginseng. So from now on, the results I have got could be used as the data which show the theoretical basis on the prescriptions.

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Basic Studies on Developing Equipment for Waterless Transportation of Live Fish (활어의 무수 수송 장치 개발을 위한 기초적 연구)

  • CHO Young-Je;KIM Yuck-Yong;LEE Nam-Geoul;CHOI Yeung-Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.5
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    • pp.501-508
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    • 1994
  • This study was undertaken to get basic data for the cold-waterless transportation of live fish. The optimal cold temperature of plaice, Paralichthy olivaceus was determined by checking the changes of dissolved oxygen and ammonia in the sea water and the survival times during storage at various temperatures. After determination of optimal temperature for transportation, the changes of serum components and muscle components of live plaice were also carried out during storage at cold($5^{\circ}C$)-waterless conditions and the recovery conditions($10\%$ density at $15^{\circ}C$). At higher storage temperature, decreases in dissolved oxygen and the increases in ammonia in seawater were observed. In addition, the survival time was short at low temperature($0^{\circ}C\;and\;3^{\circ}C$). Almost all of the serum components(hemoglobin, glucose, LDH, GOT and GPT) of live plaice gradually increased during storage in cold-waterless conditions, and then those values decreased to the initial levels after $3{\sim}10hrs$ storage in conditions of recovery. The concentration of ATP in the muscle steadily decreased during storage in cold-waterless conditions. The contents of ADP and IMP seemed to be directly related to the extent of ATP breakdown. ADP and IMP thus showed a gradual increase during storage. The level of lactate in the muscles gradually increased during these storage times, also. On the other hand, the levels of those components in the muscle entirely recovered to their original levels within $3{\sim}6hrs$ storage after they were returned to conditions of recovery. The ratio of ATP to the ATP and its related compounds${ATP/(ATP+ADP+AMP+IMP){\times}100}$ in the muscle showed $45\%$ after 18hrs storage in cold-waterless conditions. Otherwise, ratios returned to their original levels within $3{\sim}6hrs$ of storage in recovery conditions.

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Induction of Physiological Sex-Reversal and Gynogenetic Diploid in Rainbow Trout, Oncorhynchus mykiss (무지개송어의 생리학적 성전환과 자성발생 2배체 유도)

  • Lee, Cheul-Ho;Kim, Dae-Jung;Jeong, Chang-Hwa;Choi, Gyeong-Cheol;Lee, Chae-Sung;Kim, Dong-Soo
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1634-1639
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    • 2010
  • This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 mg of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at $10^{\circ}C$ and $13^{\circ}C$. More than 93% of the male population was produced by this method and growth of hormone-treated fish at $13^{\circ}C$ was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least $3,600\;erg/cm^2$ was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at $28^{\circ}C$ for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.

A Study on the Improvement of Welding Method for Ice Evaporator (얼음증발기 용접방법 개선에 관한 연구)

  • Lee, Jeong-Youn;Yoo, Heung-Ryol;Son, Yung-Deug
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.2
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    • pp.558-564
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    • 2021
  • The water purifier market has increased rapidly in recent years. The welding technology of the evaporator is a key component that determines the level of ice production and the cold water performance of an ice purifier. The finger type evaporator of an ice purifier can remove ice and is divided largely into an instant heat method and a hot gas method. In the hot gas type evaporator, particularly during the production process, the pinhole phenomenon inside the copper pipe and clogging problems occur intermittently when welding high-pressure pipes due to the high-temperature oxygen welding. Its use in a water purifier can cause a problem in that ice and cold water do not form, and repairs cannot be made on site. To solve this problem, in this study, a cap jig was applied to improve the welding defect of the hot gas evaporator. In addition, the oxygen welding flame size was adjusted so that the heat source could be well supplied to the cap jig, and the effectiveness was confirmed through a wave pressure test, a test, and a thermal shock test.