• The Korean Journal of Physiology and Pharmacology
• /
• 제22권1호
• /
• pp.101-110
• /
• 2018

In this study, we aimed to investigate the neuroprotective effects of caffeic acid phenethyl ester (CAPE), an active component of propolis purified from honeybee hives, on photothrombotic cortical ischemic injury in mice. Permanent focal ischemia was achieved in the medial frontal and somatosensory cortices of anesthetized male C57BL/6 mice by irradiation of the skull with cold light laser in combination with systemic administration of rose bengal. The animals were treated with CAPE (0.5-5 mg/kg, i.p.) twice 1 and 6 h after ischemic insult. CAPE significantly reduced the infarct size as well as the expression of tumor necrosis $factor-{\alpha}$, hypoxiainducible $factor-1{\alpha}$ monocyte chemoattractant protein-1, $interleukin-1{\alpha}$, and indoleamine 2,3-dioxygenase in the cerebral cortex ipsilateral to the photothrombosis. Moreover, it induced an increase in heme oxygenase-1 immunoreactivity and interleukin-10 expression. These results suggest that CAPE exerts a remarkable neuroprotective effect on ischemic brain injury via its anti-inflammatory properties, thereby providing a benefit to the therapy of cerebral infarction.

• Weed & Turfgrass Science
• /
• 제2권3호
• /
• pp.292-297
• /
• 2013

Winter turfgrass injury is one of the critical problems of many golf courses in Korea. Turfgrass loss from freezing injury due to low temperature leads to many types of damages including weed invasion, increased herbicide cost, increased soil erosion, and expensive re-establishment. Although Kentucky bluegrass (Poa pratensis L.) which is the most widely used among cool-season grasses in Korea is well known as cold tolerance species, freezing injuries to Kentucky bluegrass during winter are often found. Protecting the turfgrass crown is necessary to recover from low temperature stress in winter because shoots and roots can be recovered from the crown. Turf covers may protect the crowns from direct low temperatures and desiccation. Six different turf covers were installed to cover Kentucky bluegrass during a period of low temperatures. Turf covers had positive effects for spring green-up of Kentucky bluegrass based on the study. Applying any type of turf covers on Kentucky bluegrass resulted in an increase average and minimum temperature compared to the uncovered plot. Among turf covers, clear PVC film without holes produced the longest root length and the highest turfgrass quality.

• Korean Journal of Acupuncture
• /
• 제20권4호
• /
• pp.41-52
• /
• 2003

Objectives : Circii Herba has been used as a natural drug for the treatment of stress digestive system disease. The aim of this study is to investigate the role of Circii Herba aqua-acupuncture solution (CHAS) in experimental oxidative liver injury. Methods : In order to investigate the effects of CHAS on acute liver injury, male ICR mice were pretreated with CHAS(0.2 ml/mouse/day) at the loci of BL18 and CV12 for 6days, starved for 24hrs, and administerated acetaminophen(500 mg/kg, i.p.). After acetaminophen administeration, mice were sacrificed, and the liver was removed, rinsed with ice-cold $1.15{\%}$ KCI buffer, and homogenized at $4^{\circ}C$. Fractions(fraction Ⅰ, Ⅱ, Ⅲ) were isolated by differential centrifugation. Lipidperoxide, total SH, and glutathione(GSH) levels were measured in the Fraction Ⅰ. In addition, activities of hepatic enzyme, such as catalase, glutathione peroxidase(GSH-Px) were measured in the Fraction Ⅱ, and glutathione S-transferase(GST) was measured in the Fraction Ⅲ. Results : In vivo treatment of CHAS(BL18 and CV12) showed effective inhibition of acetaminophen induced lipid peroxidation, and showed elevations of total SH, GSH level, catalase, GSH-Px, GST activities. Conclusions : These results suggested that CHAS might suppress the formation of oxidative metabolites, and prevent acetaminophen induced hepatotoxicity.

• 한국안전학회지
• /
• 제14권4호
• /
• pp.192-198
• /
• 1999

Work-related musculoskeletal disorders constitute a major source of employee disability and lost wages. Cumulative Trauma Disorders(CTD) refers to a category of physical conditions which result from chronic musculoskeletal injury. Assessment of CTD risk in industry at early stage allows for early control, a safe environment, and a healthier workforce. In this study, the physical load of the shipping work in the cold storage warehouse were especially investigated. Employees were working with almost unnatural posture in a very restricted work space. The questionnaire and biomechanical analysis were used to evaluate the physical load. Results from analyses showed that they were sufficiently exposed to CTD due to repetition and unnatural posture. Based on the analysis, ways for improving working conditions are proposed. The analysis and proposals in this paper will serve as a basic tool for designing/redesigning working environment such as improvement of tools and equipments, design of times for work/rest cycle.

• 대한한방소아과학회지
• /
• 제13권1호
• /
• pp.167-180
• /
• 1999

Diarrhea is the most common digestive disease next to influenza especially in chidren. The most important spleen function is that of transporting and transforming food and fluids. Any spleen disharmomny will therefore always influence the digestive process, with such symptom as abdominal distention, lack of appetite and loose stools. The results were as follows: 1. The most common causes of diarrhea were cold(寒) Fire(熱) Dampness(濕) and the other causes of diarrhea were Fear(驚) 담(Phlegm) spleen-Qi defiency(脾氣虛), injury diet(傷食). 2.Treatment of oriental medicine consist of herb-medicine Cause of cold is Bujaejungtang(附子理中湯) Cause of fire is Sungbisan(醒脾散) Cause of dampness is Oryungsan(五笭散)

• 한방안이비인후피부과학회지
• /
• 제11권1호
• /
• pp.99-140
• /
• 1998

In the literatual studies on the suppurative osteomylitis(附骨疽), the results were as follows. Suppurative osteomylitis(附骨疽) is called 'Mudujur(無頭疽)', is concerned with Pyogenic osteomyelitis in western medicine. The pathogenic factors of Suppurative osteomylitis were caused by exposing to wind-cold-wetness evil, or body weakness, or wound a bodily injury caused by physical means. The symptoms of Suppurative osteomylitis was alternating episodes of chills and fever, pain on buttock or thigh in onset. The differential diagnosis of Suppurative osteomylitis was divided three parts. One was the stagnation of virulent fire-evil. Another was the deficiency of both vital energy & blood. The other was wind-cold-wetness evil. The treatments of Suppurative osteomylitis was clearing away heat-evil and wetness evil, removing blood stasis and promoting meridian. In the frequency of prescription, the most numerous prescription were Osintang(五神湯) and Hwangyunhaidoktang(黃連解毒湯), the next were Neitakganghwaltang(內托羌活湯), Taibangpungtang(大防風湯) etc.

• 한국응용곤충학회지
• /
• 제16권4호
• /
• pp.245-247
• /
• 1977

1. 동상해 (수부병)의 원인은 수확기의 강설과 저온(동상과율 수확직후 $10\%$, 피해과의 $50\%$폐기) 2. 동상해는 금팡이병류의 유발과 피해의 증대 요인(이병과율$8.7\%$, 동상과$4.8\%$, 부패감모량 $13.5\%$) 3. 발생 병해는 녹색, 청색, 회색 곰팡이와 균핵병등 (녹색$2.86\%$, 청색$2.3.\%$, 회색 $1.22\%$ 균핵기타 $1.21\%$) 4. 피해는 1톤당 $18.3\%$로 135kg/톤의 부패로 손실액 27만원톤(해태 2,000톤중 55.2백만원, 제주도 5,000톤중 135백만원) 5. 방제는 조기선과 및 축하와 예조, 약제처리 등으로 부패 억압(발병과 부패는 10일내에 증대, 예조로 10일간, 약제로 20일간 억제)

• 한국잠사곤충학회지
• /
• 제44권2호
• /
• pp.45-50
• /
• 2002

한성뽕을 수원과 춘천 2개 지역에서 수행한 지역적응시험 결과, 수량성이 높고 엽질 등이 우수하여 1998년 장려품종으로 지정되었으며 그 주요 특성은 다음과 같다. 1. 백상형(Morus alba L.)에 속하는 품종으로 암나무이며, 수형은 직립성이고 용천뽕에 비하여 가지수와 평균가지 길이는 적고 짧으며 마디길이는 비슷하다. 2. 발아기는 중뽕이며, 잎의 크기는 중형으로 잎두께는 약간 두꺼우며 타원형이다. 3. 뽕 수량은 용천뽕보다 10% 많으며, 사료가치는 용천뽕보다 비교적 우량하다. 4. 뽕나무 오갈병 저항성은 보통이고 눈마름병, 줄기마름 병 및 축엽세균병은 개량뽕보다 약간 강하다. 5. 춘추잠기 겸용 큰누에용 뽕으로 전국에 재배할 수 있다.

• 한국작물학회지
• /
• 제33권4호
• /
• pp.412-419
• /
• 1988

수도의 냉해는 그 재배기간에 저온이 발생되는 수도재배지역에서 중요한 생산저해요인으로 작용한다. 그러나 도작기간동안의 기상은 지역에 따라 다르기 때문에 발생되는 냉해양상 또한 다르게 나타난다. 일반적으로 냉해는 저수온에 의한 생육의 지연 및 저기온에 의한 불일발생 등으로 크게 나눌 수 있다. 본 연구는 내냉성 품종육성효율을 높이기 위한 유전정보를 얻고저 내냉성정도가 서로 다른 9개 품종의 이면교잡된 36조합 F$_1$, F$_2$, F$_3$, 상호여교배집단 및 모본집단을 공시하여 장해형내냉성에 대한 조합능력, 작물학적 형질과의 관련성을 검토하고 F$_2$집단의 선발효율을 평가하였다. 불임발생정도로서 평가된 장해형내냉성은 상가적 유전분산이 주종이었으나 비상가적 유전분산 역시 유의하게 나타났다. 일반적으로 내성성이 강한 모본인 M 101과 L201이 일반조합능력(GCA)이 높았는 반면, 약한 품종인 7703008에서 가장 낮았다. 장해형내냉성은 출수기, 간장 및 수당립수와 관계가 있었는데, 출수가 빠를수록, 간장이 클수록 그리고 수당립수가 많을수록 내냉성이 강한 경향이었으며, 특히 출수기의 영향이 가장 높았다. 비교적 내냉성이 강한 M101과 교배된 7조합에서 F$_2$개체 선발이 내냉성향상에 효과가 있었으며 이때 내냉성에 대한 평균유전력(Realized heritability)은 0.53이었고 저온불임에 대한 선발효율은 -9.4%였다.

• Journal of Korean Neurosurgical Society
• /
• 제46권5호
• /
• pp.479-483
• /
• 2009

Objective : A mouse model of spinal cord injury (SCI) could further increase our basic understanding of the mechanisms involved in injury and repair of the nervous system. The purpose of this study was to investigate whether methods used to produce and evaluate photochemical graded ischemic SCI in rats, could be successfully adapted to mice, in a reliable and reproducible manner. Methods : Thirty female imprinting control region mice (weighting 25-30 g, 8 weeks of age) were used in this study. Following intraperitoneal injection of Rose bengal, the translucent dorsal surface of the T8-T9 vertebral laminae of the mice were illuminated with a fiber optic bundle of a cold light source. The mice were divided into three groups; Group 1 (20 mg/kg Rose bengal, 5 minutes illumination), Group 2 (20 mg/kg Rose bengal, 10 minutes illumination), and Group 3 (40 mg/kg Rose bengal, 10 minutes illumination). The locomotor function, according to the Basso-Beattie-Bresnahan scale, was assessed at three days after the injury and then once per week for four weeks. The animals were sacrificed at 28 days after the injury, and the histopathology of the lesions was assessed. Results : The mice in group 1 had no hindlimb movement until seven days after the injury. Most mice had later recovery with movement in more than two joints at 28 days after injury. There was limited recovery of one joint, with only slight movement, for the mice in groups 2 and 3. The histopathology showed that the mice in group 1 had a cystic cavity involving the dorsal and partial involvement of the dorsolateral funiculi. A larger cavity, involving the dorsal, dorsolateral funiculi and the gray matter of the dorsal and ventral horns was found in group 2. In group 3, most of the spinal cord was destroyed and only a thin rim of tissue remained. Conclusion : The results of this study show that the photochemical graded ischemic SCI model. described in rats, can be successfully adapted to mice, in a reliable and reproducible manner. The functional deficits are correlated an increase in the irradiation time and, therefore, to the severity of the injury. The photothrombotic model of SCI, in mice with 20 mg/kg Rose bengal for 5 minutes illumination, provides an effective model that could be used in future research. This photochemical model can be used for investigating secondary responses associated with traumatic SCI.