• 한국동물학회지
• /
• 제8권2호
• /
• pp.33-36
• /
• 1965

We have carried out a study on the modification of the frequency of X-ray induced lethal and slow growing mutations by colchincine treatment before and after X-ray irradiation in Paramecium aurelia. 1. Lethal and slow-growing mutation induced by X-ray in Paramecium aurelia were reduced by colchicine treatment. 2. The effects of colchicine on the X-ray induced mutations were remarkable in the radiosensitive stages of cell division. 3. The pre-irradiation treatment with colchicine showed no significant influence on the frequency of mutations. 4. It is believed that the reduction of mutation induced by X-ray after treatment with colchicine was due to the fact that the delay of the cell division allowed more time for the pre-mutational damage to recover.

• Plant Resources
• /
• 제6권3호
• /
• pp.227-232
• /
• 2003

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Codonopsis lanceolata. Three tetraploid plants of C. lanceolata were produced from seeds which absorbed 0.1 % colchicine solution for 12 hours, and 0.5% colchicine solution for 1 and 6 hours from seedlings, respectively. But tetraploid was not produced from shoot tips treated by colchicine solution. Compared to diploid, tetraploid plants had larger stomata, but less number of stomata. Fresh weight of tetraploid plants was 1.4∼3.6 times heavier than diploid plants.

• Archives of Pharmacal Research
• /
• 제17권3호
• /
• pp.199-203
• /
• 1994

Several lines evidence indicate that microtubule depolymerization initiates DNA synthesis or enhances the effects of serum or purified growth factors in many types of fibroblasts. Yet little is known about the intracellular events responsible for the mitogenic effect of microtubule disrupting agents. The effects of antitubulin agents on DNA synthesis in sparse and dense cultures in the presence or absence of serum and possible involvement of G-proteins in their mitotic action were examined. In these studies, colchicine by itself appeared to be mitogenic only for confluent quiesecent human lung fibroblasts. In sparse culture, however, colchicine inhibited serum-stimulated DNA synthesis. Colcemid, another antitubulin agent, showed similar effects of growth inhibition and stimulation in sparse and confluent cultures while lumicolhicine, inactive colchicine, did not. The mitogenic effect of two antitubulin agents, colchicine and colcemid, was partially inhibited by pertussis toxin. These data suggest that microtubular integrity is associated with the expression of either negative or positive control on DNA synthesis and mitogenic effect of antitubulin agents may be partially mediated by pertussis toxin-sensitive G protein.

• Archives of Pharmacal Research
• /
• 제10권2호
• /
• pp.100-102
• /
• 1987

Inhibition of platelet aggregation by colchicine, thiocolchicine, O-demethylated and 3-glucosidic congeners was measured in vitro. 2-And 3-demethylated analogs of colchicine and thiocolchicine were found tobe as potent as colchicine and thiocolchicine, respectively, whereas 1-demethylated compounds were found to be considerably less active. Both glucosides, colchicoside and thiocolchicoside, were not very active in this assay. 2, 3-Didemethylcolchicine, which is much less toxic than colchicine, showed similar inhibitory effects on platelet aggregation as colchicine.

• 식물조직배양학회지
• /
• 제28권1호
• /
• pp.37-40
• /
• 2001

벼의 약배양 배지 내에 콜히친 첨가에 의한 염색체 배가 방법을 확립하기 위하여, 콜히친의 농도를 0.001∼0.1 mg/L까지 조절하여 약배양하였을 때 콜히친의 농도가 증가할수록 캘러스 형성률과 식물체 분화율은 점점 감소하였으며, 그 감소폭은 농도가 증가됨에 따라 점점 커지는 경향이었다. 자포니카 3품종과 통일형 1품종을 콜히친이 첨가되지 않은 배지에 약배양하였을 때 반수체의 출현빈도가 50%이상 높게 나타난 반면, 이배체는 40% 이하의 낮은 발생빈도를 나타내었다. 그러나 0.001 mg/L의 콜히친이 약배양 배지에 첨가될 경우 품종에 따라 다소간의 차이는 있으나, 반수체의 출현빈도는 상대적으로 감소되고 이배체의 발생 비율이 54.3∼60%로 증가하였다.

• 한국약용작물학회지
• /
• 제26권5호
• /
• pp.362-369
• /
• 2018

Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

• Applied Microscopy
• /
• 제22권2호
• /
• pp.46-65
• /
• 1992

In this study, we have made morphological and cytochemical observations to investigate the type of Golgi apparatus around the bile canaliculus. The animal (Wister, $220{\sim}250gm$) were divided into 4 groups; normal, hydrochol, colchicine and hydrochol-colchicine. The Golgi apparatus is classified into 16 different types from 4 different groups. In the normal group, we could observe 12 different types of the sixteen. Type I which showed convexed cisterns facing the bile canaliculi was most abundant of the types. In the hydrochol group, 14 types were observed. Type VII and type I showed convexed cisterns facing the bile canaliculus and were abundant. In the colchicine group, 11 different types were viewed and type XIV which showed intensely dilated cisterns without the polarity was predominant. In the hydrochol-colchicine group, we observed 3 different types. Type XIV clearly showed the highest percentage, although that type was less numerous in this group than in the colchicine group. In the hydrochol group, the Golgi apparatus showed a tendency to increase in numbers, while in the hydrochol-colchicine group the Golgi apparatus showed a tendency to decrease in numbers. The reactive products of thiamine pyrophosphatase and acid phosphatase were apparent over the distal Golgi cistern in the normal and hydrochol groups, but were decreased or not observed in the colchicine and hydrochol-colchicine groups. From the results, it is assumed that with the presence of the microtubule, Golgi cisterns are dilated with polarity after stimulation of secretion. Without the microtubule, the cistern becomes more intensely dilated and none polaric. Also the enzymes within the cisternal membrane become decreaed or absent and the Golgi apparatus decreases in numbers after activation of secretion.

• Annals of Clinical Neurophysiology
• /
• 제10권2호
• /
• pp.112-115
• /
• 2008

Colchicine is a drug used for the treatment of acute gouty arthritis or various autoimmune diseases. Gastrointestinal adverse effects such as abdominal pain and vomiting are the common side effects of the drug, but rarely myopathy has been reported to occur particularly in renal recipients who were treated concomitantly with immunosuppressants. Herein, we report a case who presented with acute myopathy after treated with colchicine for acute gouty arthritis.

• Plant Resources
• /
• 제6권3호
• /
• pp.188-194
• /
• 2003

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Platycodon grandiflorum in Campanulaceae. The most successful colchicine treatment for tetraploid production in P. grandiflorum was soaking treatment using 0.01 and 0.5% colchicine solution for 1 hour and 12 hours, respectively. Morphological characteristics of both diploid and tetraploid were similar, but tetraploid plants had more leaves. Compared to diploid, tetraploid had the larger stomata, but less number of stomata. Fresh weight of tetraploids was 20∼40% heavier than that of diploid.

• 대한임상독성학회지
• /
• 제6권2호
• /
• pp.138-141
• /
• 2008

A 47-year-old woman ingested about 12 mg of colchicine with suicidal intent. Colchicine, a highly poisonous alkaloid, is a commonly used treatment for gout, Bechet's disease, and familial Mediterranean fever. Despite the knowledge of its side effects, the risk of a significant overdose is under-appreciated. She suffered from acute multisystem toxicity, including gastrointestinal disorders, bone marrow suppression, alopecia, and probable pancreatitis, but she ultimately recovered with supportive therapy. We report a case of acute colchicine toxicity from a single overdose with a review of the literature.