• The Korea Journal of Herbology
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• v.32 no.4
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• pp.53-60
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• 2017

Objectives : Collagen decrease of Skin appears through various path ways. One of causes may be the Advanced glycation endproducts (AGEs) that combine formation of glucose and protein. The aim of this study was to explore the prevent wrinkle formation of Paeoniae radix (PR) and heated Paeoniae radix (HPR) via AGEs path way. Methods : AGEs formation inhibitory activities of PR and HPR measured using bovine serum albumin, glucose, and fructose. To evaluate the protective effects of PR and HPR in diabetic rats induced with streptozotocin (STZ) and methyl glyoxal (MGO), SD rat were distributed into four groups. Normal rats (Nor), AGEs-induced rats (Con), AGEs-induced rats treated with 100 mg/kg PR(PR), AGEs-induced rats treated with 100 mg/kg HPR (HPR). To induce AGEs, streptozotocin (50 mg/kg) was administered intraperitoneally and after 3 days administrated 100mM methyl glyoxal for 3 weeks. Results : The oral administration of HPR inhibited AGEs in skin tissues compared with PR. The increased reactive oxygen species (ROS) levels in the serum were diminished by HPR treatment. The analyses of kidney and skin tissues proteins indicated that HPR treatment effectively reduced AGEs related protein levels as compared to that by PR treatment. Also, HPR decreased anti-oxidant related protein levels in skin tissues such as catalase, glutathione peroxidase. Moreover, it inhibited the reduction of COL1A2 by decreasing MMP-1. Conclusion : Based on these results, it was suggested that PR and HPR could have Improving effects on wrinkle formation. These evidences provide useful information for the development wrinkle formation treated agent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.5
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• pp.664-670
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• 2016

Cheonggukjang (CKJ) is a Korean traditional food made of fermented soybeans. In comparison to normal intake of soybeans, Cheonggukjang has high digestibility with bioactive, antioxidant substances, and thrombolytic enzymes. Recent studies have reported anti-oxidant, anti-cancer, anti-inflammatory, anti-obesity activities as well as inhibitory activities against osteoporosis for CKJ. In this study, we identified the effects of CKJ on osteoblast differentiation by increasing the polyglutamic acid (PGA) content of CKJ. Alkaline phosphatase (ALP) activity and mineralization significantly increased in response to treatment with both natural CKJ (CKJ A) and PGA-increased CKJ (CKJ B). However, CKJ B exhibited higher ALP activity and mineralization than CKJ A. Real-time reverse transcription PCR demonstrated that mRNA expression of osteoblastic-associated genes such as type I collagen, alkaline phosphatase, osteocalcin, and osteopontin in C2C12 cells was significantly up-regulated by CKJ A or B treatment. These results indicate that treatment with CKJ has an anabolic effect on bone by increasing osteoblastic differentiation and ALP activity. Increasing PGA content in CKJ had a greater effect than CKJ A on up-regulation of osteoblastic gene expression in osteoblast cells.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.433-440
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• 2009

Korean sweetpotato, Ipomoea batatas, varieties were examined for their flowering response as a fundamental research to improve breeding efficiency by hybridization of this crop. Under 10.0~10.5 hours short day condition, self-rooted plants of three varieties such as Shinmi, Hongmi and Hwangmi flowered sparsely or moderately, and those of fourteen varieties including Eunmi flowered very sparsely. Under 10.6~12.0 hours day length condition, self-rooted plants of three varieties such as Shinmi, Hongmi and Hwangmi flowered very sparsely. Under the day length longer than 12 hours, none of the tested plants flowered. In all the sweetpotato plants grafted on morning glory, flowering occurred with varying degrees by varieties and day lengths. Under 10.0~10.5 hours day length, thirty two varieties including Hongmi flowered moderately or profusely, and one variety such as Mokgye 1 flowered sparsely. Under 10.6~12 hours day length, twenty three varieties including Sinchunmi flowered moderately or profusely, nine varieties including Jeungmi flowered sparsely, and Mokgye 1 didn't flower at all.

• The Plant Pathology Journal
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• v.34 no.5
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• pp.381-392
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• 2018

Clavibacter michiganensis subsp. michiganesis (Cmm) is a quarantine-worthy pest in $M{\acute{e}}xico$. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm, the disease epidemiology was monitored. Micro-Raman spectroscopy ($532nm\;{\lambda}$ laser) technique was evaluated its performance at assisting on Cmm detection through its characteristic Raman spectrum fingerprint. Our experiment was conducted with tomato plants in a completely randomized block experimental design (13 plants ${\times}$ 4 rows). The Cmm infection was confirmed by 16S rDNA and plants showed symptoms from 48 to 72 h after inoculation, the evolution of the incidence and severity on plant population varied over time and it kept an aggregated spatial pattern. The contagion process reached 79% just 24 days after the epidemic was induced. Micro-Raman spectroscopy proved its speed, efficiency and usefulness as a non-destructive method for the preliminary detection of Cmm. Carotenoid specific bands with wavelengths at 1146 and $1510cm^{-1}$ were the distinguishable markers. Chemometric analyses showed the best performance by the implementation of PCA-LDA supervised classification algorithms applied over Raman spectrum data with 100% of performance in metrics of classifiers (sensitivity, specificity, accuracy, negative and positive predictive value) that allowed us to differentiate Cmm from other endophytic bacteria (Bacillus and Pantoea). The unsupervised KMeans algorithm showed good performance (100, 96, 98, 91 y 100%, respectively).

• Journal of Oral Medicine and Pain
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• v.30 no.3
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• pp.287-300
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• 2005

Squamous cell carcinoma (SCC) in head and neck show a variability in the response to chemotherapy, even when it present with similar histological tumor type, grade, and clinical stage. The purpose of present study it to identify predictive bio-marker for the sensitivity or resistance to conventional chemotherapeutic agents, 5-fluorouracil (5-FU) and Cisplatin Oral cancer cell lines were used in present study. MTT assay was performed to evaluate the sensitivity and/or resistance to 5-FU and Cisplatin. And RT-PCR was carried out for evaluation of the mRNA expressions of various genes associated with mutation, inflammation (COX pathway), cell cycle, senescence and extracellular matrix (ECM). The molecules which are correlated with the sensitivity to 5-FU are XPA, XPC, OGG, APEX, COX-2, PPAR, Cyclin E, Cyclin B1, CDC2, hTERT, hTR, TIMP-3, TIMP-4 and HSP47. And the molecules are correlated with the sensitivity to Cisplatin are COX-1, iNOS, eNOS, PCNA, collagen 1 and MMP-9. Taken together, when choosing the appropriate chemotherpeutic agents for patients, considering the molecules which are correlated or reversely correlated is helpful to choose the resonable agents for cancer patients.

• Journal of Korea Multimedia Society
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• v.7 no.1
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• pp.132-144
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• 2004

With the rapid development of internet technology, the number of internet users and the amount of multimedia information on the internet is ever increasing. Recently, the web sites, such as e-business sites and shopping mall sites, deal with lots of image information. As a result, it is required to support content- based image retrieval efficiently on such image data. This paper proposes an intelligent image retrieval system, which adopts XML, technology. To support object-based col)tent retrieval on product catalog images containing multiple objects, we describe a multi -level metadata structure which represents the local features, global features, and semantics of image data. To enable semantic-based and content-based retrieval on such image data, we design a XML-Schema for the proposed metadata and show how to represent such metadata using XML- documents. We also describe how to automatically transform the retrieval results into the forms suitable for the various user environments, such as web browser or mobile browser, using XSLT The proposed scheme can be easily implemented on any commercial platforms supporting XML technology. It can be utilized to enable efficient image metadata sharing between systems, and it will contribute in improving the retrieval correctness and the user's satisfaction on content-based e-catalog image retrieval.

• Journal of Biomedical Engineering Research
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• v.19 no.4
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• pp.333-339
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• 1998

The effect of heat treatment conditions on the properties of tungsten(W) embolization coils has been evaluated. The W embolization coils were fabricated by coiling the primary W coil with a diameter of 30.mm on a alumina rod mandrel with a diameter of 2.92mm. The secondary coils were then heat treated at 475$^{\circ}C$and 600$^{\circ}C$ for various heat treatment time ranging from 5 minutes to 36hours. The pitch distance, diameter and shape retention capability of the W embolization coils were characterized after the heat treatment. The pitch distance of the W embolization coils increased with the heat treatment time. The diameter of W emboliazation coils decreased continuously with heat treatment time. The shape retention capability of the W embolization coils increased with the heat treatment time due to an increase in elasticity by formation of tungsten oxide film on W coil surface during the heat treatment. The heat treatment condition of W embolization Col at 600$^{\circ}C$ for 20 minute was considered desirable based on the optimization of the shape retention capability, pitch distance and secondary coil diameter after heat treatment.

• Journal of Life Science
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• v.11 no.5
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• pp.483-488
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• 2001

The antimicrobial substance produced by Pseudomonas aeruginosa KLP-2 strain was purified and identified. The substance was identified as a pyocyanine by the fast atom bombardment mass(FAB-MS). In physic-chemical properties, the pyocyanine was dark blue needles, and was soluble in various organic solvents such as chlorogorm, methanol, ethanol and ethyl acetae. The pyocyanine possessed a ultraviolet absorbance spectrum in methanol, 0.1 M HCl, and chlorogorm. The maximum absorption peak of the pyocyanine showed at 318 mm in methanol. The molecular formula of the pyocyanine was determined to the $C_{13}$ H$_{10}$ N$_{2}$O and protonate molecular ion species (M+H)$^{+}$ was observed at m/z 211 by FAB-MS. The pyocyanine showed antimicrobial against Bacillus cereus, Bacillus subtilis, Micrococcus luteus, Rodococcus equi, Staphylococcus aureus, Streptococcus faecalis, E. col, Legionella pneumophila, Shigella flexneri Shigella boydii, shgella sonnei, NAG Vibrio cholerae, Vibrio parahaemolyticus, Vibro vulnificus, Yersinia enterocolitica, and Saccharomyces cerevisiae. However, Salmonella spp. Shigela dysenteriae, 3 strains of Pseudomonas aeruginosa, Klebsiela pneumoniae, and Aspergillus niger were resistant to the pyocyanine. The pyocyanine showed the highest antimicrobial activity aganist Legionella pneumophila based on the size of inhibition zone by the disk contained 0.5 $\mu\textrm{g}$ of the pyocyanine.e.

• Nuclear Engineering and Technology
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• v.27 no.4
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• pp.562-570
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• 1995

The recent ABB/CE(Asea Brown Boveri Combustion Engineering) type pressurized oater reactor-s have the on-line monitoring system, i.e., the COLSS(core operating limit supervisory system), to prevent the specified acceptable fuel design limits from being violated during normal operation and anticipated operational occurrences. One of the main functions of COLSS is the on-line monitoring of the DNB(departure from nucleate boiling) overpower margin by calculating the MDNBR(mini-mum DNB ratio) for the measured operating condition at every second. The CETOP-D model, used in the MDNBR calculation of COLSS, is benchmarked conservatively against the TORC mod-el using an inlet flow factor of hot assembly in CETOP-D as an adjustment factor for TORC. In this study, a technique to optimize the CETOP-D inlet flow factor has been developed by elim-inating the excessive conservatism in the ABB/CE's. A correlation is introduced to account for the actual variation of the CETOP-D inlet flow factor within the core operating limits. This technique was applied to the core operating range of the YongGwang Units 3&4 Cycle 1, which results in the increase of 2％ in the DNB overpower margin at the normal operating condition, compared with that from the ABB/CE method.

• Journal of Korean Academy of Nursing
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• v.25 no.2
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• pp.259-278
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• 1995

This study was done to investigate levels of physical fitness, self efficacy(SE), instrumental activities of daily living(IADL), and quality of life (QL), and their relationships among the elderly Data were collected from 47 noninstitutionalized elderly dwell-ing in their own homes and 43 institutionalized elderly living in homes for older people. The ages of the subjects were 65 years and over. The data were col lected from January 20th to February 20th, 1995. Physical fitness was measured with T.K. K dyna mometer, grip dynamometer, stop watch, and Purdue pegboard. Structured questionnaires developed by Kim by Lawton & Brody, and by Ro were adopted to measure SE, IADL, and QL, respectively. The data were analyzed using t-test, ANOVA, and Pearson correlation coefficients. The results were as follows : 1. The noninstitutionalized elderly had higher scores in such measures of physical fitness as body weight, skinfold thickness, flexibility and coordination as compared to the institutionalized elderly. 2. On the following measures of physical fitness, men showed higher levels than women ; in height when standing, height when sitting, body weight, and muscle strength. Women had more trunk flexibility than men. 3. Muscle strength was positively correlated with height when standing, height when sitting, and body weight. The following measures of physical fitness, muscle strength, flexibility and coordi nation, were negatively correlated with "up and go”. 4. The noninstitutionalized elderly had higher scores in SE(t=2.28, p<0.05), IADL(t=2.24, p<0.05), and QL(t=2.41, p<0.05) as compared to the institutionalized elderly. 5. SE was positively correlated with both IADL(r=0.41, p<0.001) and QL(r=0.54, p<0.001), and the latter two variables were a positively correlated (r=0.30, p<0.001). 6. SE was positively correlated with the physical fitness measures of height when standing, body weight, strength of leg muscles, strength of back muscles, grip strength, and level of touching the floor, but negatively correlated with "up and go”. 7. Positive correlations were revealed between IADL and the physical fitness measures of height, strength of leg muscles, level of trunk muscle extension, level of touching the floor, and coordination. The IADL was negatively correlated with “up and go”. 8. QL was positively correlated with body weight (r=0.28, p<0.01) and skinfold thickness (r=0.26, p<0.05). 9. Age was negatively correlated with the physical fitness measures of height when standing, height when sitting, “up and go”, strength of leg muscles, strength of back muscles, grip strength, level of trunk muscle extension, and coordination. 10. Age was also negatively correlated with SE (r=-0.24, p<0.05) and IADL(r=-0.22, p<0.05). The above results suggest that caring elderly in their own homes were more effective and that nursing interventions to enhance physical fitness, SE, IADL, and QL especially for the institutionalized elderly are stressed.