• Korean Journal of Medicinal Crop Science
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• v.8 no.3
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• pp.259-265
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• 2000

This study was carried out to investigate the effect of soaking time of seed disinfectant, Fludioxonil on the germination and disease occurrence of adlay (Coix lacryma-jobi L. var. ma-yuen Stapf). The germination rate was higher in the seed disinfectant treatment for 3 days followed by seed soaking for 3 days than that of other treatments under controlled condition of low temperature. The value of two experiments were 90.8%, 96.7%, repectively. And, the time of seed soaking was longer, the more seed rot was proportionly decreased. In the pot and field experiment, germination rates were somewhat low compared to that of petri dish experiment as 88.3%, 84.3%, and occurence of seedling blight showed similar results that of petri dish experiments. There was not significantly different in the yield components among treatments in the field. However, as compared with untreated plot (194kg/10a), unhulled grain yield was increased about 26% on that treatment. Based on these results, it has been thought that improvement of the germonation rate and decrease of disease occurrence of adlay were accompanied with the seed disinfectant during 3 days followed by seed soaking during 3 days before sowing.

• Korean Journal of Medicinal Crop Science
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• v.27 no.5
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• pp.339-347
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• 2019

Background: Adlay (Coix lacryma-jobi L. var. ma-yuen Stapf.) is an important medicinal and cereal crop that contains high levels of protein, fatty acids and crude fiber, as well as calcium, phosphorus, zinc, minerals, and essential amino acids. Despite its economic significance, little is known about biological activity in adlay sprouts. This study investigated the total free sugar, polyphenol and flavonoid content, and antioxidative activity in adlay sprouts at different growth stages. Methods and Results: The total free sugar and coixol content of germinated adlay sprouts were analyzed by high performance liquid chromatography. The total phenolic content was measured by the Folin and Denis, and flavonoid content by the Davis method. Antioxidative activity was tested by the 2,2-diphenyl-1-picryl hydrazyl method. All measured component, including total phenolic and coixol content ($2.71{\pm}0.02mg/g$ and $59.70{\pm}0.01mg/g$), and antioxidant capacity ($IC_{50}$; $453.93{\mu}g/m{\ell}$) were highest in 3 day old adlay sprouts. In contrast, total free sugar and flavonoid content (3.02% and $0.60{\pm}0.02mg/g$) were highest in sprouts at 5 days after sowing. In addition, there was a strong positive correlation between antioxidative activity and total polyphenol content (r = 0.902, p < 0.001). Conclusions: Adlay sprouts between 3 days and 5 days following germination contain high levels of functional components and free sugar. This study therefore suggests that adlay sprouts can be developed as a valuable health-promoting food and a good source of natural antioxidants.

• The Korea Journal of Herbology
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• v.36 no.5
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• pp.109-115
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• 2021

Objectives : 'Johyun' yulmoo which is a new variety of Coix lacryma-jobi var. ma-yuen Stapf sprout was developed and registered by Rural development administration in 2004. This variety was derived from the cross between single cross of Suwon-6 and Okayama and UCN300-25 as F1. It is characterized by early maturity, short plant height, a strong resistance, and a superior yield and is suitable for the central and northern regions. Accordingly, we were performed and evaluated single oral dose toxicity test of 'Johyun' yulmoo sprout (JYS) in Sprague-Dawley (SD) rats. Methods : Single oral dose toxicity test was performed using with male and female rats. Rats were divided into two groups: Group 1, vehicle-treated rats (Control); Group 2, JYS 5000 mg/kg-treated rats. JYS was orally administered to male and female rats at dose levels of 5000 mg/kg. Animals were monitored on the mortality, clinical signs, body weight changes, and necropsy findings for 14 days. groups : Group 1, vehicle-treated rats (Control); Group 2, JYS 5000 mg/kg-treated rats. JYS was orally administered to male and female rats at dose levels of 5000 mg/kg. Animals were monitored on the mortality, clinical signs, body weight changes, and necropsy findings for 14 days. Results : After oral treatment of JYS, we could not find any mortality at 5000 mg/kg. Compared with the control group, there were also no significant differences in clinical sign, weight changes, weight gain, and gross abnormalities in JYS 5000 mg/kg-treated group. Conclusions : Taken together, these results suggest that approximate lethal dose of JYS was considered as over 5000 mg/kg. Results from this study provide scientific evidence for the safety of JYS. Moreover, this study could be used as a basis for dose-setting data of the repeated dose 13-week oral toxicity test of JYS.

• Natural Product Sciences
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• v.16 no.4
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• pp.291-294
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• 2010

In obesity, adipocytes undergo abnormal growth characterized by increased cell numbers and differentiation. Thus, inhibition of mitogenesis of preadipocytes and their differentiation to adipocytes would be beneficial for the prevention and progression of obesity. In the present study, we attempted to evaluate antiadipogenic activity of adlay (Coix seed, the seed of Coix lacryma-jobi L. var. ma-yuen Stapf) employing preadipocytes cell line, 3T3-L1 as an in vitro assay system. Because several varieties of adlay are in use in Korea, anti-adipogenic activity of three varieties of adlay such as Sang-Gang, Jo-Hyun and Yulmu-Ilho was evaluated. All the three varieties of adlay showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Adlay, however, showed little effects on adipocyte proliferation. Further studies with interval treatment demonstrated that adlay exerted inhibitory activity on adipocyte differentiation via acting on early stage of adipogenesis. Taken together, adlay might be useful in the prevention of obesity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.4
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• pp.452-458
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• 2019

Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf (CL), which contains riboflavin and coixol, has traditionally been used to treat cancer and arthritis. However, no method for the simultaneous determination of riboflavin and coixol in CL sprouts has been established. In this study, we established and validated a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of two reference markers, riboflavin and coixol, in CL sprout extracts. CL sprouts (whole sprouts and leaves) were subjected to extraction with 70% ethanol at room temperature and at 80 ℃ under reflux conditions. The two extractions were validated with respect to specificity, accuracy, precision, and linearity. The content of the two reference markers was highest in leaves extracted under reflux conditions (riboflavin, 8.23 ± 0.32 mg/g; coixol, 5.95 ± 0.04 mg/g). We also investigated the antioxidant activity of the extracts via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺) scavenging assays. The results indicated that extracts obtained from sprouts under reflux conditions had the strongest antioxidative effects (DPPH half maximal inhibitory concentration [IC₅₀], 68.9 ± 4.1 g/mL; and ABTS, IC₅₀, 34.9 ± 0.1 g/mL). These results can serve as baseline data for the simultaneous determination of the two reference marker compounds, riboflavin and coixol, and development of functional food materials using CL sprouts.

• Korean Journal of Medicinal Crop Science
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• v.8 no.1
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• pp.79-82
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• 2000

This study was carried out to investigate the effect of seed disinfectant on leaf blight and smut in adlay. Leaf blight and smut disease in adlay are known to be seed borne. Bjpolan's cojds, pathogen causing leaf blight in adlay, was detected from 32. 7 to 45. 7 % on seeds collected from adlay cultivated farms in Yonchon province. Periods of seed infestation are $0{\sim}7$ days on the variety 'yulmu 1' and $0{\sim}20$ days on 'Yonchon jaerae'. Distribution ratio of seed disinfestation periods was that no disinfestation, $1{\sim}5$days, $6{\sim}10$days and more 11days were 20%, 20%, 45% and 15%, respectively. Frudioxonil W. S. treatment was effective on inhibition of leaf blight and smut disease when treated with infested seeds ; that is, disease control values of the fungicide are 97 and 93.4 percentage, repectively.

• Korean Journal of Medicinal Crop Science
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• v.8 no.1
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• pp.74-78
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• 2000

This study was carried out to select the effective insecticides for control of Ostrinia furnacalis(Guenee) in Yonchon, northern part of Kyunggi province, where is main producing region of adlay in Korea. The Asian corn borer in adlay field had three generations a year. The first stage of adult activity was higher from late May to middle June, the second from late July to early August and the third late September. Based on difference in control value, the relative ranks of some insecticides were lambda cyhalothrin EC > cartap hydrochloride GR > carbaryl EC. In control value by different application timing of Lambda cyhalothrin EC, yields were significantly different, that is, twice applications on second generation showed the control value of $90.6%{\sim}93.5%$. Unhulled grain yield on Lambda cyhalothrin EC treated plot on second generation was higher than that in untreatred plot by 82%.