• International Journal of Industrial Entomology and Biomaterials
• /
• v.29 no.2
• /
• pp.207-213
• /
• 2014

The structural proteins of classical swine fever virus (CSFV) consist of nucleocapsid protein C and envelope glycoprotein $E^{rns}$ (E0), E1 and E2. Among them, E2, the most immunogenic of the CSFV glycoproteins, induces a protective immune response in swine. In this study, to determine the optimal expression conditions of glycoprotein E2 using baculovirus system, we investigated the influence of insect cells and media to the expression of recombinant E2. Recombinant virus containing glycoprotein E2 coding gene was constructed with bApGOZA DNA. Expression of the glycoprotein E2 was analyzed by SDS-PAGE and Western blot analysis using anti-CSFV E2 monoclonal antibodies. Expression of glycoprotein E2 in Sf21 cells was first observed after 3 days and reached a maximum on the 5th day after infection. Furthermore, the highest levels of glycoprotein E2 expression were observed at multiplicity of infection (MOI) of 5. When three different insect cell lines (Sf21, High-Five and Se301) were tested, High-Five cells showed the highest production. In addition, four different serum-free and serum-supplemented media, respectively, were tested for the expression of glycoprotein E2 and the budded virus (BV) titers. As a result, serum-supplemented medium provided the best conditions for protein production and the BV yield.

• Journal of Microbiology
• /
• v.39 no.2
• /
• pp.95-101
• /
• 2001

In an effort to develop an efficient expression and secretion system for heterologous proteins in Aspergilius nidulans, the PCR-amplified coding sequence for alkaline pretense (AlpA) of A. oryzae was cloned into a fungal expression vector downstream of A. nidulans aicA (alcohol dehydrogenase) promoter to yield pRAAlp. Transformation of A. nidulans with pRAAlp gave stable transformants harboring various copy numbers (3 to 10) of integrated alpA gene, from among which 6 representatives were selected. On a medium containing 0.8% ammonium sulfate that represses the expression of the host's own pretense, the alcA prumoter-controlled AlpA expression was strongly induced by threonine but repressed by glucose. The level of AlpA secretion was highest (approximately 666 mU/ml) in transformant ALP6 containing the largest copy number integrated alpA. However, the level of AlpA secretion was not necessarily proportional to the copy numbers of the integrated alpA genes. The N-terminal sequence or the secreted mature AlpA was determined to be Gly-Leu-Thr-Thr-Gln-Lys-Ser and its molecular mass to be approximately 34 kDa, indicating that AlpA is properly processed by the removal of 121 N-terminal amino acids.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2000.10a
• /
• pp.39-45
• /
• 2000

It hab been proposed that CHS3-mediated chitin synthesis during the vegitative cell cycle is regulated by CHS4. To investigate direct protein-protein interaction between their coding products, we used yeast two hybrid system and found that a domain of Chs3p was responsible for interaction with Chs4p. This domain, termed MIRC3-4 (maximum interacting region of chs3p with chs4p), spans from 647 to 700 residues. It is well conserved among CHS3 homologs of various fungi such as Candida albicans, Emericella nidulans, Neurospora crassa, Magnaporthe grisea, Ustilago maydis, Glomus versiforme, Exophiala dermatitidis, Rhizopus microsporus. A series of mutaion in the MIRC3-4 resulted in no appearance of chitin ring at the early G 1 phase but did not affect chitin synthesis in the cell wall after cytokinesis. Absence of chitin ring could be caused either by delocalization of Chs3p to the septum or by improper interaction with Chs4p. To discriminate those two, not mutually exclusive, alternatives, mutants cells were immunostained with Chs3p-specific antibody. Some exhibited localization of chs3p to the septum, while others failed. These results indicate that simultaneous localization and activation Chs3p by Chs4p is required for chitin ring synthesis.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.31 no.3A
• /
• pp.267-273
• /
• 2006

This paper improves the effects of clustered OFCDM scheme considering the frequency diversity effect over a frequency selective fading channel. In OFCDM with frequency domain spreading compared to OFDM, we can increase uncorrelated symbols by frequency allocation method of correspondent symbols over the same antenna and different antenna after spreading. The simulation results show that the performance of proposed system is improved by approximately 4 dB in ${\sigma}=0.02{\mu}sec$, the performance is improved by approximately 2.5dB in large delay spread in a 12-path Rayleigh fading channel with overall the root mean squared delay spread and the maximum Doppler frequency of 20 Hz. Also, the required average received Eb/No at the average BER of $10^{-3}$ by optimum method is improved by approximately 2.0 dB, compared to that of STA-OFCDM with frequency rearrange. The new method does not require any bandwidth expansion any feedback from the receiver to the transmitter and its computation complexity is similar to clustered OFCDM.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.33 no.12C
• /
• pp.1055-1061
• /
• 2008

H.264 is the prominent video coding standard in various applications such as real-time streaming and digital multimedia broadcasting, since it provides enhanced compression performance, error resilience tools, and network adaptation. Compression efficiency of H.264 has been improved, however, it requires more computing and memory access than traditional methods. In this paper we proposed adaptive intra fast algorithm for real-time video surveillance system reducing the encoding complexity of H264/A VC. For this aim, temporal interrelationship between macroblock in the previous and the current frame is used to decide the encoding mode of macroblock fast. As a result, though video quality was deteriorated a little, less than 0.04dB, and bit rate was somewhat increased in suggested method, however, proposed method improved encoding time significantly and, in particular, encoding time of an image with little changes of neighboring background such as surveillance video was more shortened than traditional methods.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.24 no.8B
• /
• pp.1426-1437
• /
• 1999

The introduction of fast packet switching networks such as the ATM leads to the possibility of video services with constant quality. However, the fixed traffic descriptor(TD), which is defined by ATM Forum and ITU-T, can not simultaneously satisfy network efficiency and video quality. This paper presents fast resource reallocation protocol (FRRP) which can provide an effective compromise between network efficiency and video quality. This protocol reallocates the network resources and then adapts the traffic to the reallocated resources. In the proposed method, a video session is divided into several time-intervals according to its generated traffic and TD is calculated which well characterizes the traffic during the specific subdivided interval. In addition, a system model, where MPEG coding algorithm and network control are unified, is proposal and the performance of the FRRP in terms of cell loss rate(CLR) is evaluated though the simulation using real video traffic(Star Wars). Simulation results show that the FRRP has only 5 ~35% CLR of the fixed TD.

• KIPS Transactions on Software and Data Engineering
• /
• v.6 no.5
• /
• pp.229-234
• /
• 2017

Because of the invisible nature of software and the bad coding habits (bad smell) of the existing developers, there are many redundant codes and unnecessary codes, which increases the complexity and makes it difficult to upgrade software. Therefore, it is required a code visualization so that developers can easily and automatically identify the complexity of the source code. To do this, it is necessary to construct SW visualization tool based on open source software and redefine the coupling and cohesion according to the object oriented viewpoint. Specially to identify a bad smell code pattern, we suggest how to plug-in diverse parsers within our tool. In this paper, through redefining coupling and cohesion from an object oriented perspective, we will extract bad smell code patterns within source code from inputting any pattern into the tool.

• Journal of Educational Research in Mathematics
• /
• v.19 no.4
• /
• pp.565-584
• /
• 2009

This study is aimed at providing the theoretical framework of characteristics of mathematical thinking processes and structuring the thinking process patterns of the mathematical gifted students through the analysis of their cognitive thinking processes. For this purpose, this study is trying to analyze characteristics of mathematical thinking processes of the mathematical gifted students in an objective and a systematic way, by using think-aloud method. For comparative study, the analysis framework with the use of the thinking characteristic code as a content-oriented method and the problem-solving processes code as a process-oriented method was developed, and the differences of thinking characteristics between the two groups chosen by the coding system which represented the subjects' thinking processes in the form of the language protocol through thinking-aloud method were compared and analyzed.

• Journal of Biomedical Engineering Research
• /
• v.29 no.3
• /
• pp.212-221
• /
• 2008

The total hip replacement (THR) has been used as the most effective way to restore the function of damaged hip joint. However, various factors have caused some side effects after the THR. Unfortunately, the success of the THR have been decided only by the proficiency of surgeons so far. Hence, It is necessary to find the way to minimize the side effect caused by those factors. The purpose of this study was to suggest the definite data, which can be used to design and choose the optimal hip implant. Using finite element analysis (FEA), the biomechanical condition of bone cement was evaluated. Stress patterns were analyzed in three conditions: cement mantle, procimal femur and stem-cement contact surface. Additionally, micro-motion was analyzed in the stem-cement contact surface. The 3-D femur model was reconstructed from 2-D computerized tomography (CT) images. Raw CT images were preprocessed by image processing technique (i.e. edge detection). In this study, automated edge detection system was created by MATLAB coding for effective and rapid image processing. The 3-D femur model was reconstructed based on anatomical parameters. The stem shape was designed using that parameters. The analysis of the finite element models was performed with the variation of parameters. The biomechanical influence of each parameter was analyzed and derived optimal parameters. Moreover, the results of FE A using commercial stem model (Zimmer's V erSys) were similar to the results of stem model that was used in this study. Through the study, the improved designs and optimal factors for clinical application were suggested. We expect that the results can suggest solutions to minimize various side effects.

• Journal of the Institute of Convergence Signal Processing
• /
• v.6 no.1
• /
• pp.27-32
• /
• 2005

As a large quantity of Genome sequencing has happened to be done a very much a surprising speed in short period, an automatic genome annotation process has become prerequisite. The most difficult process among with this kind of genome annotation works is to finding out the protein-coding genes within a genome. The main 2 subjects of gene prediction are Eukaryotes and Prokaryotes ; their genes have different structures, therefore, their gene prediction methods will also obviously varies. Until now, it is found that among of the 231 genome sequenced species, 200 have been found to be prokaryotes, therefore, for study of biotechnology studies, through comparative genomics, prokaryotes, rather than eukaryotes could may be more appropriate than eukaryotes. Even more, prokaryotes does not have the gene structure called an intron, so it makes the gene prediction easier. Former prokaryotes gene predictions have been shown to be 80%～ to 90% of accuracy. A recent study is aiming at 100% of gene prediction accuracy. In this paper, especially in the case of the E. coli K-12 and S. typhi genomes, gene prediction accuracy which showed 98.5% and 98.7% was more efficient than previous GLIMMER.