• Plant Breeding and Biotechnology
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• v.5 no.4
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• pp.334-343
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• 2017

Eclipta prostrata and E. alba are annual herbal medicinal plants and have been used as Chinese medicinal tonics. Both species are widely distributed in tropical and subtropical regions as well as in Korea. Both species have similar morphological features but E. alba has smoother leaf blade margins compared with E. prostrata. Although both species are utilized as oriental medicines, E. prostrata is more widely used than E. alba. Morphological semblances have confounded identification of either species. Here, we report the complete chloroplast genomes of both species to provide an authentication system between the two species and understand their diversity. Both chloroplast genomes were 151,733-151,757 bp long and composed of a large single copy (83,285-83,300 bp), a small single copy (18,283-18,346 bp), and a pair of inverted repeats (25,075-25,063 bp). Gene annotation revealed 80 protein coding genes, 30 tRNA genes and four rRNA genes. A phylogenetic analysis revealed that the genus Eclipta is grouped with Heliantheae tribe species in the Asteraceae family. A comparative analysis verified 29 InDels and 58 SNPs between chloroplast genomes of E. prostrata and E. alba. The low chloroplast genome sequence diversity indicates that both species are really close to each other and are not completely diverged yet. We developed six DNA markers that distinguish E. prostrata and E. alba based on the polymorphisms of chloroplast genomes between E. prostrata and E. alba. The chloroplast genome sequences and the molecular markers generated in this study will be useful for further research of Eclipta species and accurate classification of medicinal herbs.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.13 no.6
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• pp.33-43
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• 2003

In this paper, we proposed a real-time watermarking algorithm to be combined and to work with a DWT(Discrete Wavelet Transform)-based image compressor. To reduce the amount of computation in selecting the watermarking positions, the proposed algorithm uses a pre-established look-up table for critical values, which was established statistically by computing the correlation according to the energy values of the corresponding wavelet coefficients. That is, watermark is embedded into the coefficients whose values are greater than the critical value in the look-up table which is searched on the basis of the energy values of the corresponding level-1 subband coefficients. Therefore, the proposed algorithm can operate in a real-time because the watermarking process operates in parallel with the compression procession without affecting the operation of the image compression. Also it improved the property of losing the watermark and the efficiency of image compression by watermark inserting, which results from the quantization and Huffman-Coding during the image compression. Visual recognizable patterns such as binary image were used as a watermark The experimental results showed that the proposed algorithm satisfied the properties of robustness and imperceptibility that are the major conditions of watermarking.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.16 no.2
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• pp.105-111
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• 2006

Cryptographic application and coding theory require operations in finite field $GF(2^m)$. In such a field, the area and time complexity of implementation estimate by memory and time delay. Therefore, the effort for constructing an efficient multiplier in finite field have been proceeded. Massey-Omura proposed a multiplier that uses normal bases to represent elements $CH(2^m)$ [11] and Agnew at al. suggested a sequential multiplier that is a modification of Massey-Omura's structure for reducing the path delay. Recently, Rayhani-Masoleh and Hasan and S.Kwon at al. suggested a area efficient multipliers for modifying Agnew's structure respectively[2,3]. In [2] Rayhani-Masoleh and Hasan proposed a modified multiplier that has slightly increased a critical path delay from Agnew at al's structure. But, In [3] S.Kwon at al. proposed a modified multiplier that has no loss of a time efficiency from Agnew's structure. In this paper we will propose a multiplier by modifying Rayhani-Masoleh and Hassan's structure and the area-time complexity of the proposed multiplier is exactly same as that of S.Kwon at al's structure for type-II optimal normal basis.

• Journal of Digital Convergence
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• v.19 no.6
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• pp.61-71
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• 2021

In a situation where the online learning is expanding due to COVID-19, the current maker education has limitations in applying it to classes. This study is to design the class of online maker education using artificial intelligence tools in elementary school. Also, it is to identify the responses to it and to confirm whether it helps improve the learner's computational thinking and creative problem solving ability. The class was designed by the literature review and redesign of the curriculum. Using interveiw, the responses of instructor and learners were identified. Pre- and post-test using corresponding sample t-test was conducted. As a result, the class consisted of ten steps including empathizing, defining making problems, identifying the characteristics of material and tool, designing algorithms and coding using remixes, etc. For computing thinking and creative problem solving ability, statistically significant difference was found. This study has the significance that practical maker activities using educational artificial intelligence tools in the context of elementary education can be practically applied even in the online environment.

• Animal Bioscience
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• v.34 no.8
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• pp.1290-1302
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• 2021

Objective: Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting the highest expression of follicle stimulation hormone receptor (FSHR) will be selected to become a hierarchal follicle. The role of lncRNA, miRNA and other non-coding RNA in chicken follicle selection is unclear. Methods: In this study, the whole transcriptome sequencing of SY follicles with different expression levels of FSHR in Jining Bairi hens was performed, and the expression of 30 randomly selected mRNAs, lncRNAs and miRNAs was validated by quantitative real-time polymerase chain reaction. Preliminary studies and bioinformatics analysis were performed on the selected mRNA, lncRNA, miRNA and their target genes. The effect of identified gene was examined in the granulosa cells of chicken follicles. Results: Integrated transcriptomic analysis on chicken SY follicles differing in FSHR expression revealed 467 differentially expressed mRNA genes, 134 differentially expressed lncRNA genes and 34 differentially expressed miRNA genes, and sosondowah ankyrin repeat domain family member A (SOWAHA) was the common target gene of three miRNAs and one lncRNA. SOWAHA was mainly expressed in small white (SW) and SY follicles and was affected by follicle stimulation hormone (FSH) treatment in the granulosa cells. Knockdown of SOWAHA inhibited the expression of Wnt family member 4 (Wnt4) and steroidogenic acute regulatory protein (StAR) in the granulosa cells of prehierarchal follicles, while stimulated Wnt4 in hierarchal follicles. Overexpression of SOWAHA increased the expression of Wnt4 in the granulosa cells of prehierarchal follicles, decreased that of StAR and cytochrome P450 family 11 subfamily A member 1 in the granulosa cells of hierarchal follicles and inhibited the proliferation of granulosa cells. Conclusion: Integrated analysis of chicken SY follicle transcriptomes identified SOWAHA as a network gene that is affected by FSH in granulosa cells of ovarian follicles. SOWAHA affected the expression of genes involved in chicken follicle selection and inhibited the proliferation of granulosa cells, suggesting an inhibitory role in chicken follicle selection.

• Journal of Korean Elementary Science Education
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• v.40 no.2
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• pp.127-144
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• 2021

The purpose of this research was to conduct grounded-theory-based explorations on the types of motivation that make pre-service elementary school teachers learn science and on their type-based science-learning processes. One hundred thirty-two pre-service elementary school teachers' motivation types were analyzed, and amongst them, 12 were selected as the subjects to observe their science-learning processes to which grounded theory applied. As a result of analyzing their science-learning motivation types, it was found that the majority belonged to the type "accurators", followed in descending order by the types "directors", "explorers", and "coordinators". Coding various phenomena that appeared in their science-learning processes made it possible to derive 30 categories from them according to the grounded-theory paradigm model elements. Based on such categories derived, analysis could be made on their science-learning process flows by motivation types, according to the grounded-theory paradigm model. For example, the "accurators" were attending science lectures or reading science books to learn science knowledge and how to teach it, from a sense of obligation they took for granted as elementary school teachers. Although their experiences of science-learning processes could not be from pure intentions, due to the teacher certification examination, curriculum, or other environmental factors, they were found to have new perspectives on science with their individual efforts and participations.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.5
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• pp.1666-1689
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• 2021

The red line of Permanent Basic Farmland is the most important part in the "three-line" demarcation of China's national territorial development plan. The scientific and reasonable delineation of the red line is a major strategic measure being taken by China to improve its ability to safeguard the practical interests of farmers and guarantee national food security. The delineation of Permanent Basic Farmland zoning (DPBFZ) is essentially a multi-objective optimization problem. However, the traditional method of demarcation does not take into account the synergistic development goals of conservation of cultivated land utilization, ecological conservation, or urban expansion. Therefore, this research introduces the idea of artificial immune optimization and proposes a multi-objective model of DPBFZ red line delineation based on a clone selection algorithm. This research proposes an objective functional system consisting of these three sub-objectives: optimal quality of cropland, spatially concentrated distribution, and stability of cropland. It also takes into consideration constraints such as the red line of ecological protection, topography, and space for major development projects. The mathematical formal expressions for the objectives and constraints are given in the paper, and a multi-objective optimal decision model with multiple constraints for the DPBFZ problem is constructed based on the clone selection algorithm. An antibody coding scheme was designed according to the spatial pattern of DPBFZ zoning. In addition, the antibody-antigen affinity function, the clone mechanism, and mutation strategy were constructed and improved to solve the DPBFZ problem with a spatial optimization feature. Finally, Tongxu County in Henan province was selected as the study area, and a controlled experiment was set up according to different target preferences. The results show that the model proposed in this paper is operational in the work of delineating DPBFZ. It not only avoids the adverse effects of subjective factors in the delineation process but also provides multiple scenarios DPBFZ layouts for decision makers by adjusting the weighting of the objective function.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.30 no.6
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• pp.1131-1139
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• 2020

The static analysis of the source code is to find the remaining security weaknesses for a wide range of source codes. The static analysis tool is used to check the result, and the static analysis expert performs spying and false detection analysis on the result. In this process, the amount of analysis is large and the rate of false positives is high, so a lot of time and effort is required, and a method of efficient analysis is required. In addition, it is rare for experts to analyze only the source code of the line where the defect occurred when performing positive/false detection analysis. Depending on the type of defect, the surrounding source code is analyzed together and the final analysis result is delivered. In order to solve the difficulty of experts discriminating positive and false positives using these static analysis tools, this paper proposes a method of determining whether or not the security weakness found by the static analysis tools is a spy detection through artificial intelligence rather than an expert. In addition, the optimal size was confirmed through an experiment to see how the size of the training data (source code around the defects) used for such machine learning affects the performance. This result is expected to help the static analysis expert's job of classifying positive and false positives after static analysis.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.506-514
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• 2020

4-Hydroxybenzyl alcohol (4-HB alcohol) is one of the major active components of Gastrodia elata Blume, with beneficial effects on neurological disorders such as headache, convulsive behavior, and dizziness. Here, we developed a metabolically engineered Corynebacterium glutamicum strain able to produce 4-HB alcohol from 4-hydroxybenzoate (4-HBA). First, the strain APS963 was obtained from the APS809 strain via the insertion of aroK from Methanocaldococcus jannaschii into the NCgl2922-deleted locus. As carboxylic acid reductase from Nocardia iowensis catalyzes the reduction of 4HBA to 4-hydroxybenzaldehyde (4-HB aldehyde), we then introduced a codon-optimized car gene into the genome of APS963, generating the GAS177 strain. Then, we deleted creG coding for a putative short-chain dehydrogenase and inserted ubiCpr encoding a product-resistant chorismate-pyruvate lyase into the pcaHG-deleted locus. The resulting engineered GAS355 strain accumulated 2.3 g/l 4-HB alcohol with 0.32 g/l 4-HBA and 0.3 g/l 4-HB aldehyde as byproducts from 8% glucose after 48 h of culture.

• Knowledge Management Research
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• v.21 no.4
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• pp.1-19
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• 2020

Recently, implementation of telemedicine in Korea has been conducted as one of pilot projects with public health doctors. To manage and expand telemedicine services sustainably, it is necessary to understand the positions of various stakeholders. The purpose of this study is to observe and describe major phenomena around stakeholders related to telemedicine to capture major issues following the implementation of telemedicine. This study interviewed 24 research participants, including public health doctors who participated in the pilot project for telemedicine services. As a result of analyzing the interview manuscript using the grounded theory method, totally 68 concepts were derived at the coding stage, and 19 sub-categories, 11 categories, were proposed through the categorization process. In addition, through the elicitation concepts and categories of this study, conflicts among stakeholders were explained, conceptual models that presented the process of resolving conflicts, and five types of stakeholders were proposed. This study has theoretical and practical implications in that it captures and describes important issues from stakeholders in telemedicine services. The results of this study are expected to give some hints for problem solving to all of the stakeholders who wish to successfully build telemedicine services.