• 생약학회지
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• 제33권1호통권128호
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• pp.21-28
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• 2002

Antidiabetic effects of the acid hydrolysate of silk fibroin were investigated by oral administration to animal models for diabetes mellitus, Fibroin protein was extracted from cocoon and digested to peptides of low-molecular weight range (mainly below 3,000) and amino acids by acid hydrolysis, Feeding of the fibroin hydrolysate resulted in a significant recovering effect on reduction of body weight gain and a lowering effect on blood glucose gain in streptozotocin-induced diabetic Sprague Dawley rats (STZ rats) which were used as an insulin-dependent diabetic animal model. But the body weight and blood glucose level in C57BL/KsJ-db/db mice (db/db mice), an non-insulin-dependent diabetic animal model, were not changed significantly by the feeding, On the other hand, plasma leptin levels increased according to increased feeding amount of the hydrolysate in STZ rats and db/db mice in common, It was concluded from the results that the fibroin hydrolysate might stimulate the insulin secretion by recovering or activating pancreatic ${\beta}$ cells and result in the increased plasma leptin level. It was also deduced that the antidiabetic improvements in body weight and blood glucose gain in STZ were thought to be due to the increased insulin secretion, but in db/db mice of which the diabetic symptoms were caused by insulin resistance, the stimulated secretion of insulin was unlikely to be able to change body weight and blood glucose level significantly.

• 한국잠사곤충학회지
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• 제36권1호
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• pp.37-43
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• 1994

네 종의 서로 다른 인산염을 견정련 보조제로 사용한 경우에 있어서, 정련용수 중 금속이온의 봉쇄, 정련용액의 pH 변화, 그리고 연감효과에 대하여 검토하고 아래와 같은 결과를 얻었다. 1. 칼슘경도성분에 대한 봉쇄효과는 TSPP와 SPD가 높은 봉쇄효과를 나타내었으며, SPM과 SHP는 비교적 낮은 봉쇄효과를 보였다. 2. 철성분에 대한 봉쇄효과는 SHP와 TSPP가 우수한 봉쇄능력이 있었다. 3. 4종의 인산염 중 TSPP와 pH값이 가장 높지만 비누에 비해서는 낮다. 4. 정련속의 pH값은 인산염의 종류에 따라 많은 차이가 있었으나 pH값이 클수록 정련효과가 크게 나타났다.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.273-277
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• 2004

Variation in the size of the silk filament will determine the uniformity and quality of the silk reeled. In the present study, an attempt has been made to study the filament size variation in 6 multivoltine parental breeds and 9 multivoltine ${\times}$ bivoltine hybrids in all three seasons of a year. All multivoltine breeds and multivoltine ${\times}$ bivoltine hybrids showed variation in filament size throughout its length from the outer layer to inner layer. Results of the present study indicated that the size of the filament decreased from outer to inner layer. The decrease in filament size was sudden in some breeds/hybrids whereas it was gradual in other. Relationship between filament length to that of slope, average filament size to slope, and maximum filament size to slope was determined based on regression analysis. Regression analysis revealed significant positive correlation between slope vs average filament length (r=0.92$^{**}$) in multivoltine${\times}$bivoltine hybrids. Among parental breeds, 96C showed lowest slope of the curve (b-value: -0.00428) and 96A showed highest slope of the curve (b-value: -0.00269). Among 9 hybrids, PM${\times}$N $B_4$ $D_2$ recorded lowest slope value (b-value: -0.00328) and BL24${\times}$N $B_4$ $D_2$ showed highest value for slope of the curve (b-value: -0.00234). The breed 96C, which showed lowest slope value can be utilized for future breeding programmes to breed strains with less size deviation. Three multivoltine${\times}$bivoltine hybrids viz., PM${\times}$N $B_4$ $D_2$, 96E${\times}$CSR19 and BL67${\times}$CS $R_{101}$ , which showed less slope values (b-values: -0.00328, -0.00300 and -0.00297 respectively) can be utilized for commercial exploitation to produce uniform silk.k.

• 한국잠사곤충학회지
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• 제16권2호
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• pp.77-98
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• 1974

본 연구는 제사공정에 있어서 제사성적 및 생사의 품질을 지배하는 제사용수의 수질을 조정하고 수질별로 견층 sericin 의 팽윤, 용해의 거동적 현상을 기기분석적으로 검토하였다. 이러한 기기분석적 측정결과를 기초하여 수질별 용해의 특성이 제사성적 및 생사의 품질에 미치는 영향에 대하여 비교실험하고 다음과 같은 결과를 얻었다. I) 기기분석에 따른 sericin의 성질 및 거동 1. amino acid 분석장치를 사용하여 수질별 용해 sericin 수용액의 amino acid 분석결과는 pH 3~ll에서 serine, glycine, glutamic acid 및 aspartic acid가 주로 측정되었다. 2. pH별로 조정된 수질에서 각각의 amino산의 용해량은 pH 5 부근에서 최저로 나타났고 pH 5를 중심으로 산과 알칼리로 변화하는데 따라 증가하였다. 3. 광학현미경을 사용하여 수질별로 용해한 sericin액을 관찰한 결과 액의 pH가 커지는데 따라 sericin입자의 팽윤성이 증대되었다. 4. 용수중의 경도성분이 증가하는 경우 sericin 수용도의 sericin입자는 수검하는 경향이었다. 5. 전자현미경을 사용하여 pH별 수질에서 용출된 sericin 입자를 관찰한 결과 pH가 알칼리로 변화함에 따라 팽윤도 촉진되어 입자의 크기가 증대하였다. 6. pH별로 조정한 용수에 의해 용출된 sericin수용액의 IR spectrum 에서는 파수 2100$cm^{-l}$, 1890$cm^{-1}$ /에서만 차이가 인정되었다. 7. 견직물의 sericicin 유무시는 서로 다른 X-선회절 간섭을 나타내는데 생견직물에서는 2$\theta$=14$^{\circ}$, 17$^{\circ}$, 18$^{\circ}$, 24$^{\circ}$와 연견직물에서는 2$\theta$=17$^{\circ}$, 23$^{\circ}$, 26$^{\circ}$에서 간섭 peak를 나타내었다.

• 한국잠사곤충학회지
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• 제46권2호
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• pp.54-57
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• 2004

다수성 품종으로 육성된 부흥잠은 새로운 가을누에 품종으로서 육성경위와 주요특성을 요약 하면 다음과 같다. 1. 일본종계 원종 잠149와 도입 육성한 무늬 품종이며, 중국종계 원종 잠150은 도입 육성한 강건다수성 한성무늬 누에품종이다. 2. 부흥잠의 일본종계 원종인 잠149은 조사된 주요 양적형질 중 화용비율, 견층비율 및 견사장에서 일반조합능력(GCA)이 높았고, 중국종계 잠150은 견층비율 및 견 사장에서 GCA가 높게 나타났다. 3. 2003년 추잠기 지역적응시험 결과는 부흥잠이 다수견, 다사량의 특성을 지닌 대성 잠에 비해 전견중이 무거워 고치수량이 6% 증수되었다. 4. 조사성적에서는, 견사장은 1,274 m로 대조대비 지수로 3%가 떨어지나, 섬도가 굵고 견사량이 많아 1만두 생사량이 3.68 kg/만두로 대조 대비 지수로 7%높아 다사량의 특성을 나타내었다. 5. 잠종생산능력에 있어서는 일본종계 원종 잠149의 산란수는 525개로 대성잠의 일본종계 원종인 잠125과 같은 수준이었고 중국종계 원종 잠150의 산란수는 510개로 대성잠의 잠126보다 다소 적었으나 산란성은 우수한 편이었다.

• 한국잠사곤충학회지
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• 제41권2호
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• pp.94-101
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• 1999

Twelve pure silkworm lines identified based in higher G.C.A values from a line${\times}$Tecter crossing programme, were crossed with twelve breeds showing cocoon shell ratio>22% and hybrids prepared, evaluated in a Multiple index method. Seven hydrids showing average index value>50 in all the 10 economic characters were seclcted and evaluated in the laboratory. However, two hybrids viz., A3${\times}$935E and A3${\times}$916B were finally selected based on the superiority of the breeds in one day shorter larval duration and with and with higher reeling characters compared to control KA${\times}$NB4D2. The breeding procedure involed in evolution hybrids are discussed.

• 한국잠사곤충학회지
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• 제40권2호
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• pp.97-104
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• 1998

Mainly due to limited genetic resources available and also urgent need for hybrids suitable to local conditions, conventional theories and regular methods for bivoltine silkworm breeding are not easily applicable in such a tropical country as Sri Lanka which is recently planning to scale-up the silk industry. A simplified but practical methodology was introduced to overcome such constraints. Through application of such modified informal breeding methods, two hybrids named DP0308 and DP0314 were selected for cocoon production. Details of the altered method of germplasm improvement in the silkworm are presented, along with combining ability and heritability estimates for six parental pure lines in a half-diallel cross.

• International Journal of Industrial Entomology and Biomaterials
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• 제5권1호
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• pp.131-134
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• 2002

Mulberry being the only food of silkworm, Bombyx mori L., is of great economic importance to the silk industry, The success in cocoon production mainly depends on the supply of quality leaves in sufficient quantity. In mulberry, where the economic product is leaf, the uptake of nitrogen from soil is very heavy and high responses to application of nutrients have been reported. Nitrogen supports vegetative growth particularly the leaf biomass. Variation in nitrogen harvest index and other physiological and yield contributing traits were estimated in five mulberry genotypes. Considerable variation was observed for nitrogen harvest index, protein yield per plant and harvest index. The correlation studies indicated the protein yield per plant was significantly correlated with leaf yield, nitrogen content in leaf, nitrogen harvest index and harvest index. The broad sense heritability estimates revealed that harvest index showed highest heritability (88.07%) followed by nitrogen content (82.52%), protein yield (70.28%) and nitrogen harvest index (66.52%).

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.117-120
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• 2001

In India, mulberry (Morus spp.), the sole food plant of the silkworm, Bombyx mori (Linn.), is prone to infestation by the pink mealybug, Maconellicoccus hirsutus (Green). Infestation by this pest causes apical shoot malformation, popularly known as 'tukra'. Occurrence of tukra causes an appreciable reduction in leaf yield and quality, leading to low silkworm cocoon productivity. For management of M. hirsutus (Tukra), an IPM package comprising mechanical, chemical and biological measures was demonstrated in the mulberry gardens of five Government Silk Farms in Mysore District (Karnataka, India) during 1995-96. A suppression of 76.0% in tukra incidence and 90.19% in mealybug population was recorded by employ the IPM package which led to an estimated 4,000 kg recovery in leaf yield/ha/year. The impact of IPM package in the management of M. hirsutus, the role of biocontrol agent (Cryptolaemus montrouzieri Muls.) in pest suppression and the cost-benefit analysis of the IPM package are discussed.

• 한국잠사곤충학회지
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• 제32권1호
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• pp.38-43
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• 1990

Effects of topical application of 100, 200 and 300ng/$m\ell$ of 3-indole butyric acid (3IBA) on larval parameters were studied in polyvoltine Pure Mysore breed of B. mori. Each concentration was administered independently, at 36h to III, IV ＆ V instars, at 48h to IV ＆ V instars, and at 72h to V instar larvae. Of the various dosages used, the repeated applications of loons of 3-IBA at 36h to III, IV ＆ V instars resulted in a significant increase in larval weight, silk gland weight, cocoon shell weight and fecundity and a significant decrease in larval duration and cocooning percentage when compared with the corresponding parameters of the untreated controls.