• 대한본초학회지
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• 제22권2호
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• pp.109-114
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• 2007

Objectives : A morphological classification among Sinomenium acutum, Cocculus trilobus and Aristolochia fangchi was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Fiber bundles of pericycle in S. acutum are like a crescent moon, stone cells were found among fiber bundles, and starch grain and raphides of calcium oxalate were included in parenchyma. 2. C. trilobus has the fiber bundle of pericycle resembling a crescent moon, but does not have the raphidesof calcium oxalate. 3. A. fangchi has the raphides of calcium oxalate, but does not have the fiber bundles of pericycle. 4. Stems and roots of S. acutum arc mostly similar, but only stems have the pith. Conclusion :' C. trilobus and A. fangchi can be distinguished from each other by the fiber bundles of pericycle and the raphides of calcium oxalate.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2000년도 추계 학술발표회
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• pp.21.2-21
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• 2000

• 대한본초학회지
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• 제37권4호
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• pp.9-16
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• 2022

Objectives : In the Korean Pharmacopoeia 12th edition (KP 12) and the Korean Herbal Pharmacopoeia (KHP), two authentic herbal medicines are described, namely Bang-gi (Cheong-pung-deung) and Mok-bang-gi, respectively. In China, Bun-bang-gi is also used as herbal medicine. This study was conducted to develop a molecular authentication tool for distinguishing the three herbal medicine used as Bang-gi, which are Sinomeni Caulis et Rhizoma (Rhizome of Sinomenium acutum), Stephaniae Tetrandrae Radix (Root of Stephania terandra), and Cocculi Radix (Root of Cocculus trilobus). Methods : Twelve samples of three species (four samples of S. acutum, five samples of S. tetrandra, and three samples of C. trilobus) were collected from different habitats. The sequences of internal transcribed spacer (ITS) regions were obtained and comparatively analyzed to design the species-specific sequence characterized amplified region (SCAR) primers. The specificity of each pair of SCAR primers that amplified species-specific amplicon was evaluated for establishing the singleplex and multiplex PCR assay tools. Results : The singleplex SCAR markers show discriminability in C. acutum, S. tetrandra, and C. trilobus. These SCAR markers were also efficiently authenticated three species in the multiplex SCAR amplification using single PCR reaction. Furthermore, these PCR assay methods were applicable to authenticate dried herbal medicines distributed in the markets. Conclusions : The SCAR markers and PCR assay tools help discriminate the three herbal medicines used as Bang-gi at the species levels and provide a reliable genetic method to prevent the inauthentic distribution of these herbal medicines.

• 대한본초학회지
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• 제28권5호
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• pp.1-6
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• 2013

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

• 한국자원식물학회지
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• 제6권1호
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• pp.45-51
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• 1993

Many plants collected at Japan, China, Korea, Imdonesia and South America were applied to antitumor and / or cytotoxic screening tests against Sarcoma 180 ascites in mice and / or V-79, KB, P388 cultured cells. On the course of these screening tests, alcoholic extracts of Forsythia viridissima (Oleaceae), Eurycoma longifolia(Simaroubaceae), Rubia cordifolia and R. akane(Rubiaceae), Cissampelos pareira and Abuta concolor (Menispermaceae), Nardostachys chinensis (Valerianacese), Mansoa alliaceae (Bignoniaceae), Casearia sylvestris (Flacourtiacear), Maytenus ilicifolia (Celastraceae), Hedychium coronarium (Zingiberaceae), Croton palanostigma(Euphorbiaceae), Cocculus trilobus(Menispermaceae), Ginkgo biloba(Ginkgoaceae), Alpinia galanga and Cucculus zanthorrhiza(Zingiberaceae), Evodia rutaecarpa(Rutaceae), and Periploca sepium(Asclepiadaceae) showed significant activity and their active principles were clarified. In this paper, a few antitumor substances in above plants are introduced.

• 한국가구학회지
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• 제22권2호
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• pp.132-139
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• 2011

Wood-anatomical studies on vines, one of the main materials for fork craftworks used in the past, were insufficient compared to those for trees. Therefore, it is important to identify the species of vines in order to preserve the vinery craftwork relics. In this study, the species of 31 samples (30 traditional baskets and one sculpture relic) obtained from Korea were identified. Twenty-two baskets among 30 baskets were identified as Cocculus trilobus DC, a perennial vine which has flexible xylem and thick fibers in phloem. The other baskets were identified as $Lespedeza$ spp., a shrub with tough xylem. The sculpture part was $Actinidia$ $arguta$ Planch., a perennial vine with chambered piths.

• The Korean Journal of Ecology
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• 제15권3호
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• pp.247-255
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• 1992

In order to estimate effects of air pollution for herbs in the plnus thunbergii forests arrounding onsan industrial complex, this study was surveyed. number of species of herb also had a difference over 10 species by distance at the source of air pollution, and species diversity and evennes were also increasing at remote sites in industrial complex as woody plants. At the vicinity of industrial complex, $SDR_4$ of calamagrostis arundinatios arundinacea., miscanthus sinensis and cymbopogon tortilis var. goeringii were very high, and those of lianas as cocculus trilobus, lonicera japonica and paederia scandens, and phytolacca americana were low, but because of being higher than other sites, it was able to inferred that lianas had a tolerance to air pollution. The composition of biological spectra of herbs showed to type of $H-D_1-R_{2-3}-1$ at near site in the source of air pollution and $H-D_1-R_{1-3}-t$ at the other sites, and the propotion of lians forms were relatively high at the vicinity of industril complex.

• 한국자원식물학회지
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• 제1권1호
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• pp.8-22
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• 1988

Vincristine and vinblastine isolated from Vinco spp. , and podophyllotoxine derivatives isolated from Podophyllum spp. are usefulas anticancerous components obtaned from higher plants. More thanten antineoplastic compounds are now following them as anticancerousagents from higher plants. In my laboratory, Sarcoma 180A has beenused as the first screening test. By this method, I have found outsome kinds of antineoplastic constituents from active plants extracts .For instance, bisaborane type compounds were isolated from Curcumaxanthorrhiza, one of Indonesian plants; a morphinane type compoundfromCocculus trilobus; cyclic hexapeptides from Rubia akane and R.cordiorta. Seven components having antineoplastic actirity wereisolated from Rubia spp. except. R. tinctoria. Their structures wereelucidated except RA-Vl by chemical reaction and variovs instrumentalanalysis as shown in Fig. Among of them, RA-Vll showed strong activityagainst P388 Lymphocytic leukemia, L2O, B16 melanoma, Lewis lungcarcinoma, colon 38 and Ehrlich carcinoma. RA-V revealed excellentactivity against MM2 mammary carcinoma. The· value of acute LD5O ofRA-ViI were 10. Omg/kg( iP) and 16.5mg/kg( po ) respectiveIy . Therapruticratio was 400, compared with 10 of mitomycin C. QSAR was also appliedto these compounds by elongation of ether and ester side chains atR'. Mechanism of action of RA-Vll was also investigated and wasassumed to be inhibition of protein biosynthesis .

• 한국환경복원기술학회지
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• 제16권3호
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• pp.19-30
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• 2013

This study was proposed to adaptable species according to climate change using warmth index(WI) in Cheonan-Si. RCP 8.5 was used to estimate change of warmth index(WI) depending on climate change in Cheonan-Si. Climatic change of Cheonan-Si was estimated to change from cool temperate forest central zone to warm temperate forest zone. The following plant species will survive within WI change of Cheonan-Si from 2010 to 2050 : 18 species in the tree layer including Quercus serrata, Q. variabilis, Pinus densiflora, Q. acutissima etc.; 28 species in the shrub layer including Rhus trichocarpa, Lindera obtusiloba, Zanthoxylum schinifolium etc.; 24 species in the herb layer including Oplismenus undulatifolius, Carex lanceolata, etc.; 12 species in the vine plants including Smilax china, Cocculus trilobus, etc.

• The Korean Journal of Ecology
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• 제27권6호
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• pp.335-345
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• 2004

우리나라 공업단지 중 화학비료를 생산하고 있는 온산공단주변의 해송림이 쇠퇴된 이후 그곳에 덩굴식물류의 우점현상을 평가하기 위하여 식생조사를 실시하였다. 하층식생에 덩굴식물류의 중요치는 청미래덩굴(Smilax china)이 13.2로 가장 높았으며, 인동(Lonicera japonica)이 11.7, 계요등(Paederia scandens) 11.5, 댕댕이덩굴(Cocclus trilobus)이 7.7의 순으로 높아 대기오염으로 인한 생태계 훼손지역의 1차 식생으로 나타남을 알 수 있었다. 각 조사지별 목본류의 종수와 종다양도, 최대종다양도, 균재도, 우점도를 층위별로 나타낸 결과상층, 중층 식생에서는 종수, 종다양도, 최대종다양도, 균재도, 우점도가 산림지역이 공단지 역보다 높게 나타나 공단 주변 식생의 단순함을 보였으며, 하층 식생에서는 공단지역이 산림지역보다 높게 나타났는데 이는 덩굴식물이 차지하고 있는 비율이 높은 것이 원인인 것 같다. 초본류 조사에서는 총 59종이 출현하였으며, 고사리, 기름새, 닭의장풀, 미국자리공, 멍석딸기, 억새, 실새풀 등에서 중요치가 높아 대기오염지역의 우점종으로 나타났으며, 종수, 종다양도, 최대종다양도, 균재도, 우점도에서는 지역간의 구분이 뚜렷하게 나타나지는 않았지만 공단지역이 산림지역보다는 덩굴식물의 분포가 높게 나타났다. 이는 인접한 경기화학의 영향으로 상층식생의 쇠퇴에 따른 광조건에 의하여 열대성 식물인 덩굴식물이 우점종으로 나타나 생태계의 교란으로 인한 사막화 현상에 응용할 수 있을 것으로 생각된다.