• Title/Summary/Keyword: Coat Protein

Search Result 370, Processing Time 0.027 seconds

Comparative Analysis of Coat Protein Gene of Isolates of Cucumber mosaic virus Isolated from Pepper Plants in Two GMO Environmental Risk Assessment Fields (GM 격리포장 내 고추에서 분리한 Cucumber mosaic virus 분리주들의 외피단백질 유전자 비교)

  • Hong, Jin-Sung;Park, Ho-Seop;Ryu, Ki-Hyun;Choi, Jang-Kyung
    • Research in Plant Disease
    • /
    • v.15 no.3
    • /
    • pp.165-169
    • /
    • 2009

  • Twelve Cucumber mosaic virus (CMV) isolates were isolated from genetically modified (GM) and non-GM Capsicum annuum in two GM fields, Namyangju and Anseong, and their properties were investigated in this study. Coat protein (CP) gene of the CMV isolates were synthesized by RT-PCR using genus-specific primers which designed to amplify a DNA fragment of 950 bp. Purified cDNA fragments were cloned into the pGEMT easy vector for sequence determination. Nucleotide sequences (internal 657 bp) of CMV isolates were compared with Fny-CMV CP sequences and there were no significant collection site specific sequence similarities found. When predicted amino acid sequences (219 amino acids) were compared with Fny-CMV CP amino acids sequences, there were 96.8% to 97.3% similarities found from Namyangju collections and 95.9% to 96.8% similarities from Anseong collections. The phylogenetic analysis with nucleotide sequences showed definite differences in CMVs which have been isolated from the two regions.

  • https://doi.org/10.5423/RPD.2009.15.3.165 인용 PDF KSCI

A Subgroup IB Isolate of Cucumber mosaic virus Isolated from Lagenaria leucantha var. gourda (표주박(Lagenaria leucantha var. gourda)에서 분리한 서브그룹 IB계통의 Cucumber mosaic virus)

  • Oh, Sun-Mi;Hong, Jin-Sung;Ryu, Ki-Hyun;Lee, Gung-Pyo;Choi, Jang-Kyung
    • Research in Plant Disease
    • /
    • v.15 no.3
    • /
    • pp.254-258
    • /
    • 2009

  • An isolate of Cucumber mosaic virus (CMV), called as Lag-CMV, was identified from Lagenaria leucantha var. gourda showing mosaic symptom, and its properties was compared to Fny-CMV (subgroup IA) and As-CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein gene. Lag-CMV was similar to As-CMV used as a control CMV by the induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves of N. tabacum. cv. Xanthi nc. In the cucumber and zucchini squash, Lag-CMV and As-CMV induced a mild mosaic symptoms than that of Fny-CMV. Size and shapes of local lesions on Chenophodium amaranticolor and Vigna unguiculata induced by Lag-CMV was similar those by Fny-CMV or As-CMV. In experiments of dsRNA profiles and RT-PCR analysis of coat protein gene, Lag-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using EcoRI, SalI, MspI, XhoI, and HindIII of the RTPCR products and nucleotide sequence analysis of the coat protein gene showed that Lag-CMV belong to a member of CMV subgroup IB of the same to As-CMV.

  • https://doi.org/10.5423/RPD.2009.15.3.254 인용 PDF KSCI