• Journal of Korean Society of Forest Science
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• v.107 no.1
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• pp.81-95
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• 2018

Camp Page, To minimize the negative impacts such as the contraction of the local economy and the reduction of community activities, it has designated Baekdudaegan as protect area since September 2005, also the Korea Forest Service has implemented Support Project for National Income. However, the fundamental purpose and linkage called protection of Baekdudaegan is in lacking status, also various problems were also corrected such as indiscretion business application. For this, the purpose of this research is to provide improvement method by analyzing the problems of support project for national income of Baekdudaegan through a literature research and questionnaires of the responsible officers. First, as the research results, the strengthening sense of responsibility for Baekdudaegan supporting project for National by developing the local nationals as the Baekdudaegan's protection and management agent. Second, think the local nationals as the part of Baekdudaegan and there is need for development of system that can keep income as well as culture with community. Third, By constructing governance that the local nationals and administrations can cooperate, the area activation shall be realized through the Baekdudaegan's symmetric protection and management. The research like this, ban be used as base material of business promotion that consists area development and nationals of development restricted area while protecting the Bekdudaegan later.

• Journal of Life Science
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• v.22 no.1
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• pp.62-73
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• 2012

This study was carried out to investigate the genetic relationship of Flammulina velutipes with other species. The ribosomal DNA cluster containing 4 rRNA genes from F. velutipes 4154 were sequenced. The length of the rDNA cluster sequence was estimated at 7,403 bp long and consisted of 1,806 bp of SSU rDNA, 245 bp of ITS 1 region, 159 bp of 5.8S rDNA, 308 bp of ITS 2 region, 3,402 bp of LSU rDNA, 1,400 bp of IGS 1 region, and 83 bp of 5S rDNA. The F. velutipes 4154 genes were contained in the rDNA cluster of F. velutipes in the order of SSU rDNA - ITS 1 - 5.8S rDNA - ITS 2 - LSU rDNA - IGS 1 - 5S rDNA. The phylogenetic relationships of 20 strains of Tricholomataceae and Physalacriaceae were analyzed by conducting distance analysis using the Neighbor-joining (NJ) method. The 20 strains used in this study were divided into three groups and the strains of the genus Flammulina were related very closely to strains of Physalacria bambusae.

• Journal of Korean society of Dental Hygiene
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• v.12 no.3
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• pp.611-620
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• 2012

Objectives : This study evaluated the changes in surface roughness of denture base resin according to the types of denture cleansers. Methods : A denture base resin(Vertex RS, Dentimax, Netherland) was used. Two toothpaste(Antiplaque, Bukwang, Korea; 2080, Aekyung, Korea) and a kitchen detergent(Trio, Aekyung, Korea) were used as a denture cleanser. The specimens were put on the V8 crossbrushing machine(Sabri enterprises, Downers grove, IL, USA) to reproduce toothbrushing and the toothbrushes were flat, round end and soft type. The surfaces of denture base resin specimens were observed by profilometer(SJ-400, MITUTOYO, Japan) and SEM(S-3000N, Hitachi Co., Ibaraki, Japan). Results : 1. According to the result of measuring surface roughness, there was statistically significant difference in Ra, Rq, and Rz(p<0.05). 2. As for Ra, Rq and Rz, Antiplaque toothpaste showed the highest roughness, and there was significant difference from other groups(p<0.01). 2080 toothpaste, Trio, and distilled water were classified as the same group. 3. According to the result of observation with the SEM, the surfaces of the Antiplaque toothpaste group after toothbrushing showed the greatest roughness, and the surfaces of 2080 toothpaste, Trio, and distilled water groups were rough in order. Trio and distilled water had the surfaces similar to those before toothbrushing. Conclusions : Studies to compare the efficacy of denture management methods and examine the effects of denture cleansers on denture materials will be helpful for dental hygienists and dentists providing patients with proper information and education. And it will be also useful for denture users' oral health.

• Journal of Plant Biotechnology
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• v.47 no.3
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• pp.218-226
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• 2020

This report describes methods for selecting informative single nucleotide polymorphisms (SNPs), and the development of an online Solanaceae genome database, using 234 tomato resequencing data entries deposited in the NCBI SRA database. The 126 accessions of Solanum lycopersicum, 68 accessions of Solanum lycopersicum var. cerasiforme, and 33 accessions of Solanum pimpinellifolium, which are frequently used for breeding, and some wild-species tomato accessions were included in the analysis. To select tag-SNPs, we identified 29,504,960 SNPs in 234 tomatoes and then separated the SNPs in the genic and intergenic regions according to gene annotation. All tag-SNP were selected from non-synonymous SNPs among the SNPs present in the gene region and, as a result, we obtained tag-SNP from 13,845 genes. When there were no non-synonymous SNPs in the gene, the genes were selected from synonymous SNPs. The total number of tag-SNPs selected was 27,539. To increase the usefulness of the information, a Solanaceae genome database website, TGsol (http://tgsol. seeders.co.kr/), was constructed to allow users to search for detailed information on resources, SNPs, haplotype, and tag-SNPs. The user can search the tag-SNP and flanking sequences for each gene by searching for a gene name or gene position through the genome browser. This website can be used to efficiently search for genes related to traits or to develop molecular markers.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.8
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• pp.1263-1269
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• 2013

This study was conducted in order to investigate the inhibitory effects of natural materials on histamine production in mackerel. Antimicrobial activities on Photobacterium phosphoreum of medicinal herbs and seaweeds were investigated using the paper disc assay and MIC (minimum inhibitory concentration) test. According to the results, Sargassum sagamianum and Ecklonia cava ethanol extracts exhibited antibacterial activity. In particular, Sargassum sagamianum ethanol extract showed excellent antibacterial activity at 0.015625 mg/mL by the MIC test. Anti-histamine release activities of natural materials were further investigated by examining their inhibitory effects on histidine decarboxylase (HDC) activity in the crude enzyme preparation from Photobacterium phosphoreum. The ethanol extracts of Ecklonia cava and Eisenia bicyclis exhibited the strongest HDC inhibitory activity, with 32% and 22%, at a concentration of 1 mg/mL, respectively. Therefore, natural materials may reduce histamine poisoning through decrease of histamine production in mackerel.

• Korean Journal of Veterinary Service
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• v.41 no.1
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• pp.29-39
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• 2018

In this study, we developed a sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid visual detection of foot-and-mouth disease virus (FMDV) circulated in Korea. The RT-LAMP was completed in 40 min at $62^{\circ}C$ and the results of the assay were directly detected by naked eye without any detection process. The assay specifically amplified all 7 serotypes of FMDV RNAs but not amplified other viral and cellular nucleic acids. The sensitivity of the RT-LAMP was $10^2$, $10^3$ and $10^3TCID_{50}/mL$ for serotype O, A and Asia 1 FMDV, respectively, which was comparable to conventional reverse transcription polymerase chain reaction (RT-PCR) and relatively lower than that of real time quantitative RT-PCR (qRT-PCR). Clinical evaluation of the RT-LAMP using different serotypes of Korean and foreign FMDV strains showed a 100% (35/35) agreement with the results of the RT-PCR and qRT-PCR. These results indicated that RT-LAMP assay developed in this study could be a valuable diagnostic method for FMDV monitoring and surveillance.

• Journal of Technology Innovation
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• v.19 no.2
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• pp.153-184
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• 2011

This paper investigates what kind of technological partner firms want to cooperate with in terms of partner location. Two geographical factors are considered. One is geographical proximity, given the tradeoff between the effectiveness of knowledge spillovers in proximity and diverse knowledge absorption from geographically distant partners. The other is how many other firms are co-located with potential partners because it is known that clustering regions can create more technological outputs. Analysis on 2008 Korea Innovation Survey data finds that partner proximity is the single most important factor in choosing a cooperation partner. While firms that are located in a region crowded with related industries prefer proximate partners, others that are surrounded by unrelated industries are more likely to cooperate with distant partners. The findings suggest that geographical proximity matters in partner selection because it not only stimulates knowledge spillovers but also reduces costs involving R&D cooperation such as monitoring costs and information costs. Moreover, firms take into consideration both the benefits and risks of clustering regions. If there are so many unrelated firms that they create agglomeration diseconomies such as congestion costs and unintentional knowledge leakages, firms are more likely to try to find their cooperation partners in other regions.

• Journal of Animal Science and Technology
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• v.53 no.5
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• pp.475-480
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• 2011

This study was conducted to investigate the effects of different feeding level of TDN (Total Digestible Nutrients) on the generation of main greenhouse gases such as carbon dioxide and methane in Hanwoo cows. The diet TDN (kg) adjusted to achieve ADG of 0 g/day (Control), 200 g/day (T1), and 400 g/day (T2) of the maintenance level TMR (Total Mixed Ration) delivered twice a day at 08:30 and 17:30. Cow are housed in a respiration chamber and the environmental temperature was maintained at $20^{\circ}C$. The gases were measured for 24 hours using the multi-detector instrument Mamos-300. The analyzed methane emissions of T1 and T2 were 33.5% and 69.6% higher than control, respectively, and the carbon dioxide emissions were 21.1% and 40.6% higher than control. Also, the hourly pattern of carbon dioxide and methane production were showed very similar emission. Gas production showed peak after 1 hour of feeding and this gap was wider in the afternoon than in the morning hours. It is clearly conducted that $CO_2$ and $CH_4$ emissions were different by limited intake levels of feed.

• Journal of Conservation Science
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• v.25 no.3
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• pp.323-333
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• 2009

The Jincheonsagongnimaaeyeoraeipsang is a great stone relief Buddha in Goryeo Dynasty, transmitting sculptural styles of the Silla Kingdom. The Buddha was carved on the biotite granite basement, and was undergone cleaning treatment in 2007. The basement rock was opened in plenty cracks bringing out structural instability. And the top of the basement rock was colonized by trees obstructing sunshine and raising humidity. As a result of failure analysis, the basement rock of the Buddha had a major possibility of wedge failure in the parts of the face, hands and cloths. Therefore, the cracks were filled up with epoxy resin L-50, and titanium bars and wire ropes were applied to bind cracked rock blocks. The surface of the crack filler was colored by granite and talc powder with inorganic pigment and L-30. The crack meters were installed on the stone relief Buddha to monitor further behavior, lastly.

• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.540-544
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• 2008

In this study, response surface methodology was employed in order to optimize the limonin and nomilin extraction conditions from citron seed. The independent variables were extraction temperature, extraction time, and shaking velocity. The yield of limonin and nomilin increased with increased extraction temperature and time. The extraction effects on the limonin and nomilin were higher at extraction temperature (p<0.01). The predicted extraction conditions were validated through actual experiments. The predicted conditions were as follows: $49.7^{\circ}C$ of extraction temperature, 3.3 hr of extraction time, and 400.6 rpm of shaking velocity for the maximum limonin contents (353.9 mg/100 g) and $50.3^{\circ}C$, 3.5 hr, and 399.9 rpm for the maximum nomilin contents (214.5 mg/100 g). The experimental values of limonin and nomilin contents at the predicted conditions were 338.8 and 219.5 mg/100 g, respectively. The predicted values at the optimized conditions were acceptable when compared to the experimental values.