• Mycobiology
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• 제45권2호
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• pp.84-89
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• 2017

Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separate vectors. Subsequently, in the two vectors containing the nourseothricin and phosphinothricin resistance cassettes were inserted eGFP and mCherry expression cassettes, respectively. These new vectors were then introduced independently into A. tumefaciens and used to transform M. robertsii either in independent events or in one single co-transformation event using an equimolar mixture of A. tumefaciens cultures. The number of transformants obtained by co-transformation was similar to that obtained by the individual transformation events. This method provides an additional strategy for the simultaneous insertion of multiple genes into M. robertsii.

• Journal of Information Processing Systems
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• 제19권3호
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• pp.302-309
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• 2023

Artificial intelligence (AI) plays a crucial role in the intelligent development of China's power system. It is also an important part of the digital development of the power grid. The development of AI determines whether the digital transformation of China's power system can be successfully implemented. Therefore, this paper discusses the digital transformation of the power grid based on AI technologies. The author has established a digital evaluation index system to reflect the development of the power grid in one province. Both qualitative and quantitative methods have been adopted in the analysis, which delves into the economic effectiveness, quality, and coordination of power grid development in the province in a comprehensive way. Results show that, to meet the needs of the power grid's digital transformation, the correlation coefficient between the power grid's development and the province's overall coordination has been increasing in recent years.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.157-167
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• 2011

In order to establish a reliable and highly efficient method for genetic transformation of pepper, a monitoring system featuring GFP (green fluorescent protein) as a report marker was applied to Agrobacteriummediated transformation. A callus-induced transformation (CIT) system was used to transform the GFP gene. GFP expression was observed in all tissues of $T_0$, $T_1$ and $T_2$ peppers, constituting the first instance in which the whole pepper plant has exhibited GFP fluorescence. A total of 38 T0 peppers were obtained from 4,200 explants. The transformation rate ranged from 0.47 to 1.83% depending on the genotype, which was higher than that obtained by CIT without the GFP monitoring system. This technique could enhance selection power by monitoring GFP expression at the early stage of callus in vitro. The detection of GFP expression in the callus led to successful identification of the shoot that contained the transgene. Thus, this technique saved lots of time and money for conducting the genetic transformation process of pepper. In addition, a co-transformation technique was applied to the target transgene, CaCS (encoding capsaicinoid synthetase of Capsicum) along with GFP. Paprika varieties were transformed by the CaCS::GFP construct, and GFP expression in callus tissues of paprika was monitored to select the right transformant.

• 한국항행학회논문지
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• 제21권1호
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• pp.132-137
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• 2017

본 논문에서는 로봇의 구조나 보행 상황을 반영해 하나의 보행 패턴을 다른 보행 패턴으로 변환하게 해주는 영모멘트 점 (ZMP; zero moment point)와 질량 중심 (CoM; center of mass)의 실시간 변환 방법을 제안한다. 일반적으로 휴머노이드 로봇은 높이와 질량과 같은 자체적인 구조 특성을 가지고 있다. 이러한 구조적 특성으로 인해 인간 또는 휴머노이드 로봇으로부터 측정되거나 생성되어진 CoM / ZMP 보행 패턴을 다른 로봇에 직접 적용하는 것은 어렵다. 이를 위하여 간단한 휴머노이드 로봇 모델인 cart-table model을 사용해 보폭의 길이, 보행 시간, CoM 높이 변화에 따라 보행 패턴의 특성을 분석한다. 그러한 분석으로부터 변환 방정식을 유도하고 시뮬레이션을 통해 제안된 방법을 검증한다.

• 한국육종학회지
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• 제41권3호
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• pp.252-260
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• 2009

1. 본 연구에서는 공동배양 배지에 Agrobacterium 성장 억제물질인 silver nitrate를 첨가하고 변온과 여과지처리를 추가하여 공동배양 기간을 7일로 늘였으며, 또한 항산화 물질 3종을 공동배양 배지에 첨가하여 세포의 oxidative burst를 최소화함으로써 벼 형질전환효율을 높일 수 있었다. 또한 이 방법을 적용하여 형질전환이 어려운 품종을 대상으로도 형질전환 식물체를 작성할 수 있었다. 2. 벼 형질전환체의 70%에서 도입유전자 수가 1copy인 것으로 나타나, 적은 수의 유전자가 안정적으로 도입됨을 확인 하였다. 3. 이러한 결과를 바탕으로, 새로운 공동배양 방법을 사용하여 우수한 농업적 형질을 가진 벼 육종 소재 및 품종을 신속하게 개발할 수 있을 것으로 기대된다.

• 한국분말야금학회:학술대회논문집
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• 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part2
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• pp.879-880
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• 2006

Effect of phase transformation and grain-size variation of hot-pressed cobalt on its dry sliding wear was investigated. The sliding wear test was carried out against glass (83% $SiO_2$) beads at 100N load using a pin-on-disk wear tester. Worn surfaces, cross sections, and wear debris were examined by an SEM. Phases of the specimen and wear debris were identified by an XRD. Thermal transformation of the cobalt from the hcp $\varepsilon$ phase to the $\gamma$ (fcc) phase during the wear was detected, which was deduced as the wear mechanism of the sintered cobalt.

• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.230-234
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• 2019

Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.

• 한국해양공학회지
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• 제37권5호
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• pp.190-197
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• 2023

In the shipbuilding and marine industry, digital transformation activities are promoted primarily by large shipyards. However, bottlenecks are observed across value chains, and digital transformation effects are reducing because of the cost and technical challenges encountered by supplies. In this study, we proposed a win-win cooperation model for large, small, and medium-sized companies using digital transformation based on the characteristics of the shipbuilding and marine industry through case studies. We investigated the digital transformation progress in German and Korean small and medium-sized enterprises (SMEs). In addition, we identified information-sharing methods and management challenges encountered in enterprise resource planning and manufacturing execution systems in the collaboration process of pipes, panels, blocks, etc. of SMEs that are suppliers of a Korean shipyard, and clarified communication by building a platform based on a common format between shipyards and suppliers. Further, we proposed a standard model of a digital transformation system for enhancing the collaboration between large companies and suppliers and proposed a basic plan including strategies to efficiently and effectively build a digital transformation system based on the standard model.

• 한국전자거래학회지
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• 제15권3호
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• pp.163-181
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• 2010

최근 온난화 현상에 따른 범세계적 위기가 초래됨에 따라 선진국을 중심으로 교토의정서가 체결되었으며, 이를 계기로 온실가스 규제가 본격화되기 시작하였다. 또한 교토의정서의 교토유연성체계라는 시장메커니즘이 도입되어 온실가스 배출에 대한 소유권이 설정되고, 수급에 따라 배출권 가격이 형성되는 등 새로운 무형의 자원을 거래하는 탄소시장이 등장하게 되었다. 학계에서는 탄소배출효과를 측정하는 방법론과 이를 탄소배출권의 경제적 비용으로 전환하고자 하는 연구들이 태동하기 시작하였다. 본 연구에서는 전자정부 정보화사업 중 하나인 G2B 시스템을 통해 전통적인 조달업무 프로세스가 온라인화 됨에 따라 저감되는 탄소 배출저감효과 즉, e-transformation화에 따른 탄소저감효과를 측정하는 ECRE(Evaluation of $CO_2$ Reduction in E-transformation)모델과 절차를 제시하고자 한다. ECRE 모델은 세계 기후협의체(IPCC)의 방법론을 근간으로 하여 크게 '이동 수단에 따른 탄소저감효과', '종이 문서 절감에 따른 탄소저감효과' 2가지의 탄소배출량을 측정하고 이를 탄소배출권의 경제적 비용으로 환산한다. 또한 본 연구에서는 탄소저감효과를 효과적으로 측정하기 위하여 5단계와 10개의 세부절차로 구성된 절차를 제시하였다. 본 연구에서 제시한 ECRE 모델과 절차를 이용하여 정부기관은 물론 온실가스 규제를 받게 되는 에너지 환경기업 등에서 정보화사업을 통한 탄소감축 실적의 추정 및 추가적인 정보화사업 추진으로 기대되는 탄소저감효과를 사전에 시뮬레이션해 볼 수 있는 도구로서 활용이 기대된다.

• 한국초지조사료학회지
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• 제20권2호
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• pp.103-108
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• 2000

To produce of transgenic orchardgrass, the seed-derived calli of orchardgrass (Dactylis glomerata L.) co-cultivated with Agrobacterium turnefaciens EHAlOl harboring binary vector pIG121-Hm were selected with hygromycin and then transferred onto N6 regeneration medium containing 1 rngl l of NAA, 5 rngl l of kinetin, 250 rngl l of carbenicillin and 50 mg/ l of hygromycin. The efficiency of transformation was differed on cultivars, that is, 'Potomac' appeared 12% of transformation efficiency while 'Amba' did 5.5%. The addition of acetosyringone during co-cultivation was a key to successhl transformation of orchardgrass. Transgene fragments were identified by PCR analysis and the constitutive expression of GUS gene was confirmed by Northern blot analysis. (Key words : Acetosyringone, Agrobacterium tumefaciens, Orchardgrass (Dactylis glomerata L.), Transformation)