• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.168-168
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• 2022

According to the 5th Climate Change Report, global average temperature in 2081~2100 will increase 1.8℃ based on RCP 4.5 and 3.7℃ based on RCP 8.5 from the current climate value (IPCC Working Group I AR5). As temperature is expected to increase due to global warming and the intensity and frequency of rainfall are expected to increase, damage to crops is expected, and countermeasures must be taken. This study intends to evaluate rice growth in terms of nitrogen utilization efficiency according to future climate change conditions. In this experiment, Oryza sativa cv. Shindongjin were planted at the SPAR facility of the NICS in Wanju-gun, Jeollabuk-do on June 10, and were planted and grown according to the standard cultivation method. Cultivation conditions are high temperature, high CO₂ (current temperature+4.7℃·CO₂ 800ppm), high temperature (current temperature+4.7℃·CO₂ 400ppm), current climate (current tempreture·CO₂ 400 ppm). Nitrogen was varied as 0, 9, 18 kg/10a. The N content and C/N ratio of all rice leaves, stems, and seeds increased at high temperature, and the N content and C/N ratio decreased under high temperature and high CO₂ conditions com pared to high temperature. Compared to the current climate, NUE increases by about 8% under high temperature and high CO₂ conditions and by about 2% under high temperature conditions. This seems to be because the increase in temperature and CO₂ induced the increase in biomass. ANUE related to yield decreased by about 70% compared to the current climate under high temperature conditions, and decreased by about 45% at high temperature and high CO₂, showing a tendency to decrease compared to high temperature. This appears to be due to reduced fertility and poor ripening due to high temperature stress. However, as the nitrogen increased, the number of ears and the number of grains increased, slightly offsetting the production reduction factor.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2006.11a
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• pp.127-130
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• 2006

Coenzyme Q10(CoQ10) is a biological quinine compound that is widely found in living organisms including yeast, plants, and animals. CoQ10 has two major physiological activities:(a)mitochondrial electron-transport activity and (b )antioxidant activity. Various clinical applications are also available: Parkinson's disease, Heart disease, diabetes. Because of its various application filed, the market size of CoQ10 is continuously expanding all over the world. A Japanese company, Nisshin Pharma Inc. is the first industrial producer of CoQ10(1974). CoQ10 can be produced by fermentation and chemical synthesis. In several companies, these two methods are used for the production of CoQ10:chemical synthesis - Yungjin, Daewoong, Nishin Parma; fermentation - Kaneka, Kyowa, Yungjin, etc. Researchs in microbial production of CoQ10 have several steps: screening of producing microorganisms, strain development, fermentation process, purification process, scale-up process, plant production. Several strategies are available for the strain development : Random mutation and screening, directed metabolic engineering. For the optimization of fermentation process, various conditions (nutrient, aeration, temperature, culture type, etc.) are considered. Purification is one of the most important step because the quality of final products entirely depends on its purity. The production cost will be reduced and the quality of the CoQ10 will be impoved by continuous researches in strain development, fermentation process, purification process.

• 한국약용작물학회:학술대회논문집
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• 2006.11a
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• pp.127-130
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• 2006

Coenzyme Q10(CoQ10) is a biological quinine compound that is widely found in living organisms including yeast, plants, and animals. CoQ10 has two major physiological activities:(a)mitochondrial electron-transport activity and (b)antioxidant activity. Various clinical applications are also available : Parkinson's disease, Heart disease, diabetes. Because of its various application filed, the market size of CoQ 10 is continuously expanding all over the world. A Japanese company, Nisshin Pharma Inc. is the first industrial producer of CoQ10(1974). CoQ10 can be produced by fermentation and chemical synthesis. In several companies, these two methods are used for the production of CoQ10:chemical synthesis - Yungjin, Daewoong, Nishin Parma; fermentation - Kaneka, Kyowa, Yungjin, etc. Researchs in microbial production of CoQ10 have several steps: screening of producing microorganisms, strain development, fermentation process, purification process, scale-up process, plant production. Several strategies are available for the strain development : Random mutation and screening, directed metabolic engineering. For the optimization of fermentation process, various conditions (nutrient, aeration, temperature, culture type, etc.) are considered. Purification is one of the most important step because the quality of final products entirely depends on its purity. The production cost will be reduced and the quality of the CoQ10 will be impoved by continuous researches in strain development, fermentation process, purification process.

• Journal of the Korean Wood Science and Technology
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• v.47 no.2
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• pp.210-220
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• 2019

This work aimed to evaluate the influence of culture conditions on laccase production in the co-culture of wood-rotting fungus with Trichoderma sp. The effects of infection extent, infection time, and culture filtrate of Trichoderma sp. on the laccase production by wood-rotting fungus in co-culture were examined. T. rubrum LKY-7 and T. longibrachiatum were selected as fungi which are effective in co-culture for laccase production. A significant increase in laccase activity was observed when T. rubrum LKY-7 was co-cultured with T. longibrachiatum in glucose-peptone liquid medium, yielding an increase of more than 5 times in laccase activity, as compared with control. Laccase production by T. rubrum LKY-7 during co-culturing was significantly influenced by the infection extent and the infection time of T. longibrachiatum. Maximal laccase activity was obtained when T. rubrum LKY-7 culture was infected by T. longibrachiatum after 3 days of cultivation at an inoculum size ratio of 0.5 to 1. The addition of culture filtrate or autoclaved mycelium of T. longibrachiatum to T. rubrum LKY-7 culture did not significantly enhance laccase production by T. rubrum LKY-7 as compared with control (mono cultures of T. rubrum LKY-7).

• Journal of Institute of Convergence Technology
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• v.12 no.1
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• pp.19-23
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• 2022

Interest in hydrogen production to respond to climate change is increasing. Until now, hydrogen has been mainly produced through the SMR (Steam Methane Reforming) process using natural gas. A large amount of CO₂ is emitted in the hydrogen production process through SMR, and the gas flow including CO₂ generated in the SMR process has different characteristics for each emission source, so it is important to apply a suitable CO₂ capture process. In the case of PSA tail gas or synthesis gas, the applicability of an amine-based process has been confirmed or demonstrated close to a commercial level. However, in the case of the flue gas generated from the reformer, it is still difficult to apply the conventional amine-based process because the partial pressure of CO₂ is relatively low. Energy-saving innovative absorbents such as phase separation absorbents can be a solution to these difficulties.

• KSBB Journal
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• v.31 no.4
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• pp.246-255
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• 2016

Co-production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) was suggested as an innovative strategy to overcome several limitations occurring in the single production of 3-HP from glycerol. In this study, two new isolates of Klebsiella pneumoniae, which produce less lipopolysaccharide (LPS) thus considered less pathogenic than K. pneumoniae DSM 2026, were compared and evaluated for their potential for the co-production of 3-HP and 1,3-PDO. The newly isolated strains showed significantly faster sedimentation rate than DSM, which should be beneficial for downstream processing. Analysis of genome sequences of the isolates confirmed the presence of all genes necessary for glycerol assimilation, 1,3-PDO production and biosynthesis of coenzyme $B_{12}$. Co-production yield was highest under anaerobic condition while cell growth was highest under aerobic condition. Both strains showed similarly good performance for the co-production although J2B gave the slightly higher co-production yield of 0.80 mol/mol than GSC021 (0.75 mol/mol). The evaluation of the newly developed strains presented here should be useful in designing similar evaluation experiments for other microorganisms.

• Journal of Microbiology and Biotechnology
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• v.5 no.5
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• pp.292-296
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• 1995

Production of poly($\beta$-hydroxybutyrate-co-$\beta$-hydroxyvalerate)[poly(HB-co-HV) from glucose and propionic acid was studied in a two-stage fed-batch fermentation using Alcaligenes eutrophus NCIMB 11599. When either glucose became sufficient or the feeding rate of propionic acid decreased, production of poly(HB-co-HV) increased but concomitantly resulted in a reduced fraction of HV. During the copolymer accumulation stage, the specific production rate of hydroxyvalerate (HV) increased up to 0.013 (g-HV/g-RCM/h) but it decreased as propionic acid was accumulated. Control of the propionic acid concentration in the medium, therefore, is considered to be one of the most important operating parameters for production of poly(HB-co-HV) with a higher HV fraction. A high titre of poly(HB-co-HV) (85.6 g/I) with HV fraction of 11.4 mol$%$ could be obtained in 50 h by controlling the propionic acid concentration at 1 to 4 g/I.

• Journal of Energy Engineering
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• v.13 no.1
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• pp.1-11
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• 2004

Gasification is the essential technology that can meet the interim hydrogen demand of large quantity before entering the hydrogen economy. Although the hydrogen production that is based upon the pure renewable energy like wind and solar power will eventually prevail, the interim mass production of hydrogen for the next ten to twenty years will come from the technologies that can demonstrate the economic feasibility in production cost with a high potential in minimizing CO$_2$ generation and in improving plant efficiency. Particularly, feedstock such as natural gas, coal, petroleum residual oil, wastes, and biomass appears to be utilized in Korea as hydrogen source, at least during the short and medium period of time, owing to the advantage in production cost. Because one of the main reasons behind the recent hydrogen issue is the reduction requirement of CO$_2$ that would be controlled according to the climate change protocol, hydrogen production technologies must be developed to yield the minimal CO$_2$ generation.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.904-910
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• 2010

LCA (Life Cycle Assessment) carried out to estimate carbon footprint and to establish of LCI (Life Cycle Inventory) database of pepper production system. Pepper production system was categorized the field cropping (redpepper) and the greenhouse cropping (greenpepper) according to pepper cropping type. The results of collecting data for establishing LCI D/B showed that input of fertilizer for redpepper production was more than that for greenpepper production system. The value of fertilizer input was 2.55E+00 kg $kg^{-1}$ redpepper and 7.74E-01 kg $kg^{-1}$ greenpepper. Amount of pesticide input were 5.38E-03 kg $kg^{-1}$ redpepper and 2.98E-04 kg $kg^{-1}$ greenpepper. The value of field direct emission ($CO_2$, $CH_4$, $N_2O$) were 5.84E-01 kg $kg^{-1}$ redpepper and 2.81E+00 greenpepper, respectively. The result of LCI analysis focussed on the greenhouse gas (GHG), it was observed that the values of carbon footprint were 4.13E+00 kg $CO_2$-eq. $kg^{-1}$ for redpepper and 4.70E+00 kg $CO_2$-eq. $kg^{-1}$ for greenpepper; especially for 90% and 6% of $CO_2$ emission from fertilizer and pepper production, respectively. $N_2O$ was emitted from the process of N fertilizer production (76%) and pepper production (23%). The emission value of $CO_2$ from greenhouse production was more higher than it of field production system. The result of LCIA (Life Cycle Impact Assessment) was showed that characterization of values of GWP (Global Warming Potential) were 4.13E+00 kg $CO_2$-eq. $kg^{-1}$ for field production system and 4.70E+00 kg $CO_2$-eq. $kg^{-1}$ for greenhouse production system. It was observed that the process of fertilizer production might be contributed to approximately 52% for redpepper production system and 48% for greenpepper production system of GWP.

• Microbiology and Biotechnology Letters
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• v.51 no.1
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• pp.59-68
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• 2023

Xylanase is an industrially relevant enzyme used for the production of xylobiose and xylose. Various methods are used to enhance the microbial yield of xylanase. In the present study, co-culturing of Bacillus subtilis and Bacillus pumilus were investigated using submerged fermentation for xylanase production, which was markedly increased when sal, sagwan, newspaper, wheat bran, and xylan were used as single carbon sources. Maximum xylanase production was reported after 5 days of incubation in optimized media at pH 7.0 and 37℃, resulting in 2.69 ± 0.25 µmol/min by coculture. The 1:1 ratio of sal and sagwan in optimized production media was shown to be suitable for xylanase synthesis in submerged fermentation (SMF). In comparison to mono-culture using B. pumilus and B. subtilis, co-culturing resulted in an overall 3.8-fold and 2.15-fold increase in xylanase production, respectively.