• Applied Biological Chemistry
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• v.24 no.2
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• pp.105-111
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• 1981

Growth responses of Brevibadterium ammoniagenes BA 17-2, which had been obtained by the treatment of several mutagens in our previous report, were investigated to select the preliminary optimal concentrations of phosphate, $Mg^{++}$, $Mn^{++}$ and thiamine for the production of 5'-XMP aminase. In this experiment it was shown that the concentration of phosphate in the medium has an important effect on the growth of microorganism. Using the medium containing 0.2% of $MgSO_4{\cdot}7H_2O$, 3mg/l of $MnSO_4{\cdot}H_2O$and $1\;mg/l$ of thiamine-HCl, the maximum cell mass was obtained at the concentration of 0.4% of $KH_2PO_4$ and $K_2HPO_4$, respectively. Above the concentration of these phosphates, cell growth was inhibited as the phosphate concentration increased to 1%, but the inhibition was overcome by the addition of 1% of $MgSO_4{\cdot}7H_2O$ and 3mg/l of thiamine-HCl. The 5'-XMP aminase activity was also influenced by the concentration of phosphate, $Mg^{++}$, $Mn^{++}$, and thiamine. In addition, the optimal culture pH and temperature for the enzyme activity were found to be 6.8 and $32^{\circ}C$, respectively.

• Journal of the Korean Chemical Society
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• v.32 no.4
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• pp.323-332
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• 1988

The bacteria containing urease convert each molecule of urea into two molecules of ammonia and one molecule of carbon dioxide gas. Bacterial electrodes have been constructed by immobilizing the Proteus vulgaris on an ammonia and a carbon dioxide gas-sensors, and were investigated for the effects of pH, temperature, buffer solution, bacterial amounts and interferences, and life time. NH3-bacterial electrode based on ammonia gas-sensor had linearity in the range of $7.0{\times}10^{-4}\;-\;3.0{\times}10^{-2}$M urea in pH 7.4, 0.05M phosphate buffer at $25^{\circ}C$ with a slope of 116.7 mV/decade. While $CO_{2-}$bacterial electrode based on carbon dioxide gas-sensor bad linearity in the range of $7.0{\times}10^{-4}\;-\;5. 0{\times}10^{-2}$M urea in pH 7.0, 0.1M phosphate buffer at $30^{\circ}C$with a slope of $45.4{\times}45.7mV/decade$. As the clinical application, urea in urine was determined by these devices and this result was compared with spectrophotometric method. Consequently, these electrodes could be used for the analysis of many samples because of simplicity, rapidity and convenience of the experimental procedure.

• Analytical Science and Technology
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• v.5 no.4
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• pp.425-431
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• 1992

The concentration and determination of trace phosphate ion was studied by $La(OH)_3$ coprecipitaiton. Phosphate ions in 1.0L samples were coprecipitated with lanthanium hydroxide at pH 9.5 adjusted with ammonia solution. The precipitates were floated with the aid of mixed surfactant(1:8 sodium oleate/sodium dodecyl sulfate) and nitrogen gas bubbles. The floated precipitate was collected in suction flask from the solution. The precipitate were washed with dil. ammonia solution and dissolved in sulfuric acid. The phosphate ion in the concentrated solution was finally determinated by UV/VIS spectrophotometry using the molybdenium blue method. The proposed method could be applied to the determination of phosphate ion in tap water and river water.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.spc
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• pp.47-50
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• 2009

Nutrient losses, especially nitrogen and phosphorus, in agricultural runoff can contaminate surface and ground water, leading to eutrophication. Thus, erosion control is crucial to minimizing nutrient losses from agricultural land. Assessments of various erosion control practices were carried out under various cropping system, soil management practices, and slope conditions by means of a lysimeter study and under artificial rainfall. Soil and nutrient losses were monitored in a small agricultural field to evaluate the soil conservation practices. Nutrient losses occur in runoff and leachate (dissolved nutrient) and in sediments (particulate nutrient). Dissolved nitrates accounted for the majority (about 90%) of nitrate transport within the soil. Particulate phosphate in sediments represented the majority (60% to 67%) of phosphate transport. Recently, engineering and agronomic erosion-control practices haver been used to reduce erosion problems in fields on slopes. These practices reduced soil loss, runoff, and nutrient loss to 1/6, 1/2,and 1/3 their original levels, respectively. Bioavailable particulate phosphate in sediments represents a variable but longterm source of phosphate for algae. Dissolved nitrate and phosphate are immediately available for algal uptake, so reducing fluxes of these nutrients should also reduce the risk of eutrophication.

• Food Science and Preservation
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• v.13 no.6
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• pp.803-807
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• 2006

Browning of commercial Doenjang product during storage and marketing is a main concern in the industry. There have been many studies on the improvement on the quality of Doenjang during storage. In this study, phosphate and gas absorber treatment was introduced in manufacturing of Doenjang to improve the color of commercial Doenjang during storage. Doenjang treated with Phosphate and 9as absorber (sample C). Doenjang teated with Phosphate only (sample B), and the control (sample A) were prepared and their physicochemical and sensory properties were determined during storage at $30^{\circ}C$ for 28 days. Sample C was the best in terms of color as well as sensory evaluation resulting in preventing browning of Doenjang during storage. These result suggest that commercial production of Doenjang should introduce phosphate and gas absorber treatment to improve the quality of Doenjang, and to prevent undesirable browning reaction during storage and marketing.

• Korean Journal of Environment and Ecology
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• v.29 no.5
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• pp.777-784
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• 2015

This study verified the correlations between vegetation factors, such as the number of individual species and species diversity, and soil factors in Chamaecyparis obtusa (CO) stands in Jeollanam-do. Also, the site environmental factors that affect the annual growth of CO ring width were analyzed. Positive correlations were found between the species diversity index and Cation Exchange Capacity (CEC), available phosphate, and exchangeable $K^+$ (P<0.01). In addition, strong positive correlations were also found between the number of species that appeared in the study site and CEC, available phosphorus, exchangeable $K^+$ and exchangeable $Mg^{2+}$ (P<0.01). Tree ring growth showed strong correlations with the nutrient holding capacity and fertility of soil, including available phosphate, exchangeable $K^+$, CEC, and electrical conductivity (P<0.01). The explanatory variables of tree ring growth in CO were composed of exchangeable $K^+$, organic matter content, and soil pH. The regression model had a high level of explanatory power, 74.4%. In this model, the annual growth of CO ring width increased when exchangeable $K^+$ and organic matter content were higher but decreased when soil pH was lower. According to the analysis, it is found that the annual growth of CO ring width was significantly affected by soil fertility, including available phosphate, exchangeable $K^+$, CEC, and electrical conductivity. In addition, the soil fertility of CO stands seems to be significantly affected by the supply of fallen leaves from the understory vegetation of CO.

• Korean Journal of Environmental Agriculture
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• v.33 no.3
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• pp.220-230
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• 2014

BACKGROUND: Excessive metals in the soil have become one of the most significant environmental problems. Phytoremediation has received considerable attention as a method for restoring the contaminated soils. The microbes having remarkable metal tolerance and plant growth-promoting abilities could also play a significant role in remediation of metal-contaminated soils, because bioaugmentation with such microbes could promote phytoextraction of metals. Therefore, the present study was focused on evaluating the phytoextraction of heavy metals (Co, Pb and Zn) in Helianthus annuus (sunflower) induced by bioaugmentation of a phosphate solubilizing bacterium. METHODS AND RESULTS: A phosphate solubilizing bacterium was isolated from metal-contaminated soils based on the greater halo size (>3 mm) with solid NBRIP agar medium containing 10 g glucose, 5 g $Ca_3(PO_4)_2$, 5 g $MgCl_2{\cdot}6H_2O$, 0.25 g $MgSO_4.7H_2O$, 0.2 g KCl, 0.1 g $(NH_4)_2SO_4$ in 1 L distilled water. Isolated bacterial strain was assessed for their resistance to heavy metals; $CoCl_2.6H_2O$, $2PbCO_3.Pb(OH)_2$, and $ZnCl_2$ at various concentrations ranging from $100-400{\mu}g/mL$ (Co, Pb and Zn) using the agar dilution method. A pot experiment was conducted with aqueous solutions of different heavy metals (Co, Pb and Zn) to assess the effect of bacterial strain on growth and metal uptake by Helianthus annuus (sunflower). The impact of bacterial inoculation on the mobility of metals in soil was investigated under laboratory conditions with 50 mL scaled polypropylene centrifuge tubes. The metal contents in the filtrate of plant extracts were determined using an atomic absorption spectrophotometer (Perkinelmer, Aanalyst 800, USA). CONCLUSION: Inoculation with Enterobacter ludwigii PSB 28 resulted in increased shoot and root biomass and enhanced accumulation of Co, Pb and Zn in Helianthus annuus plants. The strain was found to be capable of promoting metal translocation from the roots to the shoots of H. annuus. Therefore, Enterobacter ludwigii PSB 28 could be identified as an effective promoter of phytoextraction of Co, Pb and Zn from metal-contaminated soils.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.3
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• pp.255-263
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• 1996

This study was conducted to evaluate the application effects of three different granular fused magnesium phosphate products on soybean in a volcanic ash upland soil(Namweon series) of Cheju island. They were two domestic products(Kyunggi Chemical Industrial Co., LTD, Pungnong Biryo Industrial Co., LTD) and an imported Chinese product that were manufactured from different added materials. A powder fused magnesium phosphate. a single superphosphate(water soluble phosphatic fertilizer) and a fused superphosphate(mixed water soluble phosphate and 2% citric acid soluble phosphate) were presented as check fertilizers. Yield of soybean was the highest in the Pungnong product of granular fused magnesium phosphate. the next was the Chinese product imported and the Kyunggi product was the lowest but there was no statistical significance among the three granular products of fused magnesium phosphate. There was no clear tendency between yield and yield components of soybean plant however, the effects on the number of mainstem nodes, number of branches nodes and number of pods per plant were observed in the increased yield treaments. Phosphate concentration in stems and pods of soybean plant at harvesting stage was higher than those in stems of soybean plant during growing period. Uptake amounts and recovery rates of phosphate in stems and pods of soybean plant at harvesting stage were similar with the yield increasing tendency on soybean. In the changes of soil pH at different periods, the application of phosphatic fertilizers was increased soil pH. Soil pH in Chinese product was higher than domestic products, but it was similar to single superphosphate. The available silicate concentrations of soil were higher in the plot of Pungnong product than Chinese product.

• Journal of Life Science
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• v.19 no.8
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• pp.1033-1038
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• 2009

Maximal productions of gellan by Sphingomnas paucibilis NK2000 from 20 g/l glucose and 10 g/l soybean pomace were 7.46 g/l in a flask and 7.35 g/l in a 7 l bioreactor, when the initial pH of media was 6.8. Maximal production of gellan in a 7 l bioreactor under pH control by sodium hydroxide was 8.42 g/l, whereas that under control by potassium phosphate was 8.50 g/l. The optimal concentration of potassium phosphate in a medium for production of gellan by S. paucibilis NK2000 was found to be 5.0 g/l. Maximal production of gellan in a medium containing 5.0 g/l potassium phosphate without pH control was 8.93 g/l in a 7 l bioreactor. In this study, a simple process without pH control was developed to enhance the production of gellan, with optimized concentration of potassium phosphate in the medium.

• Journal of Sensor Science and Technology
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• v.4 no.3
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• pp.22-29
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• 1995

Lithium ionic solid electrolyte was synthesized at $60^{\circ}C$ in $N_{2}$ ambience by sol-gel method, using tetraethyl orthosilcate, lithium methoxide, zirconium n-propoxide, and tributyle phosphate as precursors. The prepared material was dried and crushed into powder, and it was pressed into disk type samples. These samples were sintered at $900^{\circ}C{\sim}1100^{\circ}C$ for 50 hours. The physical characteristics of the samples were investigated by TG/DTA, SEM, AES and XRD methods. $CO_{2}$ sensor based on lithium ionic solid electrolyte was fabricated and its operational characteristics were measured. The sensing characteristics of the sensor sintered at $1000^{\circ}C$ shows the variation of e.m.f. about $35{\sim}63\;mV/decade$ for the variation of $CO_{2}$ concentration at $200^{\circ}C{\sim}300^{\circ}C$ of operating temperature, and good linearity for $300{\sim}6000\;ppm$.