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A Fundermental Study on Stabilization in Municipal Waste Landfill Site (도시폐기물 매립지의 안정화에 관한 기초연구)

  • 김은호;김순호
    • Journal of Environmental Health Sciences
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    • v.27 no.1
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    • pp.56-62
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    • 2001
  • The investigation was carried out to analyze the generation and the composition of landfill gas generated from inserted pipe wells into the underground by boring operation and also study the undecomposed waste characteristics by open-cut test at S. waste landfill site in Pusan city. Pilot test was conducted for stabilization. The experimental results from this study were summerized as follows. ; Since COD matter was easuer decomposed than COD matter for continuously biological stabilization in underground, it seemed that BOD and CO $D_{Mn}$ were in the range of 854~4,813mg/$\ell$ and 1,156~6,977mg/$\ell$ and their ratio were generally as high as 0.55~0.74. As C $H_4$ compositions of generated gas were measured in the range of 37.36~60.1%, we could know that C $H_4$ gas was actively generated. Organic matters by open-cut test averaged 13.4~16.6% at each landfill layer, and considering rate of combustible compositions(36.2~66.5%) for landfilling wastes, they have been actively decomposed. The measured and theoretical values of generated gas in waste landfill site were almost similar to C $H_4$ 50.0% and 53.4%, $CO_2$ 39.63% and 45.24%, and after 0.5$^{\circ}C$ with heavy depth and long landfill period. From the results of pilot test for stabilization, after 180 days organic matters were actively decomposed beyond 2.2 times in facultative aerobic lystimeter(B) to exsiting anaerobic lysimeter(A). Therefore, it seemed that landfill site was of benefical to the conversion of facultative aerobic for stabilization.

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Agrobacterium- mediated Genetic Transformation and Plant Regeneration of Sweetpotato (Ipomoea batatas) (Agrobacterium 매개에 의한 고구마 형질전환 및 식물체 재분화)

  • Lim, Soon;Yang, Kyoung-Sil;Kwon, Suk-Yoon;Paek, Kee-Yoeup;Kwak, Sang-Soo;Lee, Haeng-Soon
    • Journal of Plant Biotechnology
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    • v.31 no.4
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    • pp.267-271
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    • 2004
  • Transformed sweetpotato (Ipomoea batatas (L.) Lam. cv. Yulmi) plants were developed from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105/pCAMBIA2301 harboring genes for intron $\beta$-glucuronidase (GUS) and kanamycin resistance. Transient expression of GUS gene was found to be higher when embryogenic calli were co-cultivated with Agrobacterium for 2 days. The co-cultured embryogenic calli transferred to selective MS medium containing 1mg/L 2,4-D, 100mg/L kanamycin, and 400mg/L claforan. These embryogenic calli were subcultured to the same selection medium at 4 weeks interval. Kanamycin-resistant calli transferred to hormone-free MS medium with kanamycin gave rise to somatic embryos and then converted into plantlets in the same medium. Southern blot analysis confirmed that the GUS gene was inserted into the genome of the sweetpotato plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the leaf, petiole, and vascular tissue and tip of root.

Neurotropism and Invasiveness of $\alpha-Herpes$ Virus in the Rodent (설치류에서 알파 Herpes 바이러스의 신경친화성과 침습)

  • KIM Jin-Sang;Yi Seong-Joon;Card J. Patrick
    • The Journal of Korean Physical Therapy
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    • v.9 no.1
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    • pp.59-70
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    • 1997
  • The ability of neurotropic alpha herpesviruses to replicate within synaptically linked neurons has made these pathogens valuable tools for transneuronal analysis. Recent studies suggest that unique gene products expressed by genetically engineered strains of virus may permit the use of multiple strains in complex tracing paradigms. In the present study we have examined the invasiveness of two genetically engineered strains of the swine pathogen known as pseudorabies virus(PRV). The two strains were isogenic with the attenuated Bartha strain of PRV; in one strain a lacZ reporter gene was inserted into the gC locus (PRV-BaBlu; $4.75\times10^8pfu/ml$) contrained a PRV envelope glycoprotein gene that was absent in PRV-BaBlu. Simultaneous or temporally separated sequential injection of $4\mu\ell$ of each strain into the ventral wall of the stomach produced a predictale course of retrograde synaptic infection. The results were as follows: 1. PRV-BaBlu and PRV-D infected the dorsal motor nucleus of vagus nerve(DMV) and paraventricular nucleus(PVN). 2. Invasion and replication of PRV-D occured at a faster rate than the parental strain or PRV-BaBlu. 3. PRV-D was much more virulent than PRV-BaBlu or the parental strain. 4. Co-injection of PRV-D and PRV-BaBlu produced an infection that was more virulent than that produced by the parental strain (PRV-Bartha), 5. Neurons in DMV were permissive to co-infection with PRV-D and PRV-BaBlu when they were injected simultaneously into the same site. 6. Replication of PRV-BaBlu was compromised by prior infection of the same circuit with PRV-D. 7. Prior infection of neurons with PRV-D maked them resistant to infection with PRV-BaBlu.

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Simulation of Contacts Between Wire Rope and Shell Plate of a Block for Shipbuilding Industry based on Multibody Dynamics (다물체계 동역학을 기반으로 한 와이어로프와 조선용 블록 외판 사이의 간섭 시뮬레이션)

  • Jo, A-Ra;Ku, Nam-Kug;Cha, Ju-Hwan;Park, Kwang-Phil;Lee, Kyu-Yeul
    • Korean Journal of Computational Design and Engineering
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    • v.17 no.5
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    • pp.324-332
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    • 2012
  • In this paper, a method for calculating the contact force and the frictional force caused by contacts between the wire rope and the rigid body is introduced based on multibody dynamics. And the method is applied to a simulation of contacts between the wire rope and the shell plate of a block that can occur during shipbuilding. The wire rope is composed of a number of lumped masses and the wire rope segments that connect the masses. After calculating the position of interference, we inserted a contact node into the wire rope. We then derived the equations of motion of the wire rope and the rigid body using augmented formulation based on multibody dynamics taking into account the constraints between the contact node and the rigid body. Using the equations, we were able to obtain the constraint force between the contact node and the rigid body, and calculate the contact force and the frictional force, based on which the position of the contact node was corrected. Finally, we applied our results to perform simulation of contacts between the wire rope and the shell plate of a block in order to verify the efficacy of the method proposed in this paper.

The Decarbonization of Sea Water by LD Slag

  • Han, Ki-Hyun;Kim, Hyung-Suek;M. S. Oh;Byeon, Tae-Bong
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.532-536
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    • 2001
  • LD slag, that is, a by-product of steel making process, has been mainly used as land construction materials. Recently, the seashore application of LD slag was tried in Japan and Korea but the reaction between LD slag and seawater was not studied yet. We tried to clarify the leaching reaction and/or mechanism of LD slag and the reaction between seawater and LD slag. We tried to apply these results to the decarbonization of seawater for seawater magnesia process. The high pH solution(over 12) was injected into the sea water and the pH of mixed liquid was adjusted to 9.8. This mixed solution was aged for 8 hours and the 104ppm of CO$_3$$^{-2}$ in the sea water was decreased to 23ppm with the negligible loss of $Mg^2$$^{+}$. The slag particle was directly inserted into the seawater fur practical application. The 0.5~1mm particles were suitable for decarbonization when 5 grade slags mentioned above were reacted with sea water. In this case, the content of CO$_3$$^{-2}$ in the sea water was 53 ppm with the negligible loss of $Mg^2$$^{+}$ after 8 hours aging. The direct application of slag particle fur the decarbonization of seawater takes more reaction time.ime.

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Effective Comb Type Pilot Assignment and PAPR Reduction in NC-OFDM-Based Communication System (NC-OFDM 기반 통신 시스템에서 효율적인 Comb Type Pilot 배치 방식과 PAPR 저감 기법)

  • An, Dong-Geon;Ryu, Heung-Gyoon;Lee, Seung-Jun;Ko, Dong-Kuk
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.9
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    • pp.965-970
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    • 2010
  • Because of a large number of subcarriers, the high PAPR(Peak-to-Average Power Ratio) is the major drawback of NC-OFDM system used for wireless communication system. Comb type pilot assignment is more efficient and lower computational complexity for the channel estimation than the block type pilot. However, even if the CAZAC(Constant Amplitude Zero Autocorrelation) matrix transform is used for the PAPR reduction of the data symbols, PAPR increases when the pilot is inserted in comb type with the data symbols. Therefore, in this paper, we additionally use a new SLM technique in order to lower the PAPR again even in the comb type pilot. Also, a new SLM technique suggested in this paper does not need any additional bandwidth for sending selection information for SLM. This combined method has good PAPR reduction performance and efficient data transmission.

Antisense GA 3β-Hydroxylase Gene Transferred to Rice Plants. (Antisense gibberellin 3β-hydroxylase발현 형질전환벼)

  • 강용원;윤용휘;김길웅;이인중;신동현
    • Journal of Life Science
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    • v.14 no.4
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    • pp.644-649
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    • 2004
  • During plant development, active gibberellins (GAs) control many aspects of plant growth and development including seed germination, stem elongation, flower induction, anther development and seed growth. To understand the biosynthesis and functional role of active GAs in high plants, this study investigated GA 3$\beta$-hydroxylase gene en-coding $GA_1$ and$GA_4$ catalizing last step in GA biosynthetic pathway. The antisense GA 3$\beta$-hydroxylase gene was inserted into expression vector, pIG121-Hm. Calli derived from mature seeds of rice (Oryza satiiva L. cv. Donjinbyeo) were co-cultivated with Agrohacterium tumefaciens EHA101 earring a pIG121-Hm containing hygromycin resistance ($Hyg^r$) and antisense GA 3$\beta$-hydroxylase gene. Seventeen transgenic plants obtained inhibiting GA 3$\beta$-hydroxylase. Transgenic plants had shorter plant height more than that of the Dongjinbyeo. Stable integration of antisense GA 3$\beta$-hydroxylase gene was confirmed by polymerase chain reaction of genomic DNA isolated from the leaf organs of the $T_o$ generation.

EFFECT OF OVER-EXPANSION CYCLE IN A SPARK-IGNITION ENGINE USING LATE-CLOSING OF INTAKE VALVE AND ITS THERMODYNAMIC CONSIDERATION OF THE MECHANISM

  • Shiga, S.;Hirooka, Y.;Miyashita, Y.;Yagi, S.;Machacon, H.T.C.;Karasawa, T.;Nakamura, H.
    • International Journal of Automotive Technology
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    • v.2 no.1
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    • pp.1-7
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    • 2001
  • This paper presents further investigation into the effect of over-expansion cycle in a spark-ignition engine. On the basis of the results obtained in previous studies, several combinations of late-closing (LC) of intake valve and expansion ratio were tested using a single-cylinder production engine. A large volume of intake capacity was inserted into the intake manifold to simulate multi-cylinder engines. With the large capacity volume, LC can decrease the pumping loss and then increase the mechanical efficiency. Increasing the expansion ratio from 11 to 23.9 with LC application can produce about 13% improvement of thermal efficiency which was suggested to be caused by the increased cycle efficiency. The decrease of compression ratio from 11 to 5.5 gives little effect on the thermal efficiency if the expansion ratio could be kept constant. Thus, the expansion ratio is revealed to be a determining factor for cycle efficiency, while compression ratio is no more important, which suggests the usefulness of controlling the intake charge with intake valve closure timing. These were successfully explained by simple thermodynamic calculation and thus the mechanism could be verified by the estimation.

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Comparison of three digital radiographic imaging systems for the visibility of endodontic files (근관 파일의 인지도 평가시 세 가지 디지털 방사선영상시스템의 비교)

  • Park Jong-Won;Kim Eun-Kyung;Han Won-Jeong
    • Imaging Science in Dentistry
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    • v.34 no.3
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    • pp.145-150
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    • 2004
  • Purpose: To compare three digital radiographic imaging sensors by evaluating the visibility of endodontic file tips with interobserver reproducibility and assessing subjectively the clarity of images in comparison with the x-ray film images. Materials and Methods: Forty-five extracted sound premolars were used for this study. Fifteen plaster blocks were made with three premolars each and #8, 10, 15 K-flexofiles were inserted into the root canal of premolars. They were radiographically exposed using periapical x-ray films (Kodak Insight Dental film, Eastmann Kodak company, Rochester, USA), Digora imaging plates (Soredex-Orion Co., Helsinki, Finland), CDX 2000HQ sensors (Biomedisys Co., Seoul, Korea), and CDR sensors (Schick Inc., Long Island, USA). The visibility of endodontic files was evaluated with interobserver reproducibility, which was calculated as the standard deviations of X, Y coordinates of endodontic file tips measured on digital images by three oral and maxillofacial radiologists. The clarity of images was assessed subjectively using 3 grades, i.e. plus, equal, and minus in comparison with the conventional x-ray film images. Results: Interobserver reproducibility of endodontic file tips was the highest in CDR sensor (p < 0.05) only except at Y coordinates of #15 file. In the subjective assessment of the image clarity, the plus grade was the most frequent in CDR sensor at all size of endodontic file (p < 0.05). Conclusion : CDR sensor was the most superior to the other sensors, CDX 2000HQ sensor and Digora imaging plate in the evaluation of interobserver reproducibility of endodontic file tip and subjective assessment of image clarity.

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An Experimental Study on Energy Reduction of an Exhaust Air Heat Recovery Type Outdoor Air Conditioning System for Semiconductor Manufacturing Clean Rooms (반도체 클린룸용 배기 열회수식 외기공조시스템의 에너지절감에 관한 실험적 연구)

  • Song, Gen-Soo;Yoo, Kyung-Hoon;Kang, Shin-Young;Son, Seung-Woo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.21 no.5
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    • pp.273-281
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    • 2009
  • In recent semiconductor manufacturing clean rooms, the energy consumption of outdoor air conditioning systems represents about 45% of the total air conditioning load required to maintain a clean room environment. Meanwhile, there is a large amount of exhaust air from a clean room. From an energy conservation point of view, heat recovery from the exhaust air is therefore useful for reducing the outdoor air conditioning load for a clean room. In the present work, an energy-efficient outdoor air conditioning system was proposed to reduce the outdoor air conditioning load by utilizing an air washer to recover heat from the exhaust air. The proposed outdoor air conditioning system consisted mainly of a preheating coil, an air washer, two stage cooling coils, a reheating coil, a humidifier and two heat recovery cooling coils inserted into the air washer and connected to a wet scrubber. It was shown from the lab-scale experiment with outdoor air flow of $1,000\;m^3/h$ that the proposed system was more energy-efficient for the summer and winter operations than an outdoor air conditioning system with a simple air washer.