• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.5
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• pp.860-865
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• 1997

Investigations were performed on the effects of extraction temperature, pressure, time on solubility and selectivity of egg yolk lipid and cholesterol, and color and fatty acid composition of the residue in supercritical carbon dioxide(SC-Co$_2$) extraction. Lipid and cholesterol solubility increased as the increase of Co$_2$ density and was found to strongly depend on the extraction pressure rather than the extraction temperature. The relative concentration of cholesterol in the extract increased with an increase in temperature and decreased with an increase in pressure and extraction time. extraction of dried eg yolk for 3hr at 4$0^{\circ}C$/276 bar removed 46.1% of cholesterol from the residual egg yolk with a yield of 63.2%. SC-Co$_2$ extraction produced a lighter color egg yolk with less redness and yellowness. As the extraction time increased, the resultant residual egg yolk became more saturated with fatty acids. SC-$CO_2$ extraction offers a safe, natural method for removing cholesterol from dried egg yolk.

• The Korean Journal of Food And Nutrition
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• v.12 no.4
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• pp.358-363
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• 1999

This study was conducted to evaluate the extraction methods for the determination of antioxidants in soybean oil. Recovery rates of various antioxidants in soybean oil showed similar rates as 80.4~102.1% by solvent/solvent extraction method and 89.9~106.4% by sweep co-distillation method except 46.6~61.2% of PG at corresponding spiked concentractions. The maximun recovery rates of antioxidnts were obtained when extraction time and extraction temperature used in UNITREX were 20min and 21$0^{\circ}C$ respectively. In the recovery rates with the activation of florisil when 2% ofwater was added to florisil the highest recovery rates for TBHQ, BHA, BHT were obtained by sweep co-distillation met-hod. Therefore sweep co-distillation method showed less solvent simple operation and high recovery rate compared with solvent/solvent extraction method.

• Food Science and Biotechnology
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• v.14 no.1
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• pp.6-10
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• 2005

Optimal extraction conditions such as pressures, temperatures, and modifiers on glycyrrhizin extraction from licorice were investigated using supercritical $CO_2\;(SC-CO_2)$ at 3 mL/min flow rate. Morphology of licorice tissue, after glycyrrhizin extraction, was examined by SEM, and absolute density ($g/cm^3$) measurement and glycyrrhizin content were determined by HPLC. Pure $SC-CO_2$ had no effect on glycyrrhizin extraction, but recovery of glycyrrhizin ($32.66{\pm}0.77%$) was enhanced when water was used as modifier. The highest recovery was $97.22{\pm}2.17%$ when 70% (v/v) aqueous methanol was added to 15% (v/v) $SC-CO_2$ at 50 MPa and $60^{\circ}C$. Under optimal extraction conditions, 30 MPa pressure and $60^{\circ}C$ heating temperature, glycyrrhizin recovery reached maximum ($102.67{\pm}1.13%$) within 60 min. Licorice tissue was severely damaged by excessive swelling, and absolute density of licorice residues was highest when aqueous methanol was used as a modifier.

• Food Science and Preservation
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• v.7 no.2
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• pp.177-183
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• 2000

A supercritical fluid extraction was performed for the extraction of phenolics from grape seeds which up to now have been discarded. The optimum condition for extraction process was predicted through response surface methodology using central composit experimental design. The extraction amount of grape seed phenolics was increased by increasing extraction temperature, pressure, and concentration of co-solvent (ethanol). The optimum extraction conditions were 84.83$^{\circ}$C, 51.50MPa and 1.27% ethanol. The yield of phenolics using SFE was higher with 3 folds than ethanol and 4 folds than hexane but less than 80% methanol. In the respects of food poisoning, the approved solvents were restricted to ethanol and hexane. So, SFE for extraction of phenolics could be powerful alternative method for solvent extraction.

• Fisheries and Aquatic Sciences
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• v.15 no.4
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• pp.275-281
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• 2012

Yellow croaker Larimichthys polyactis muscle oil was extracted using an environmental friendly solvent, supercritical carbon dioxide (SC-$CO_2$), in a semi-batch flow extraction process. SC-$CO_2$ was applied at temperature $35^{\circ}C$ to $45^{\circ}C$ and $150^{\circ}C$ to $250^{\circ}C$ bar of pressure. The flow rate of $CO_2$ (27.79 g/min) was constant throughout the entire 1.5 h extraction period. The oil extraction yield was influenced by the physical properties of SC-$CO_2$ at different temperatures and pressures. The extracted oil was analyzed by gas chromatography to determine the fatty acid composition. According to our results, the SC-$CO_2$ extracted oil was high in eicosapentaenoic acid and docosahexaenoic acid. In addition, the SC-$CO_2$ extracted oil showed greater stability than n-hexane extracted oil based on the peroxide value and acid value. Thus, the quality of yellow croaker oil obtained by SC-$CO_2$ extraction was slightly higher than that of oil obtained by n-hexane extraction.

• KSBB Journal
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• v.25 no.5
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• pp.437-442
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• 2010

The objective of this study was to investigate the effect of ultrasound on the extraction of oil from canola seeds when supercritical carbon dioxide ($SCCO_2$) was used as an extraction solvent. The ultrasound-assisted $SCCO_2$ extraction were carried out while varying such operating parameters as particle size of crushed canola seed, flow rate of $SCCO_2$, aspect ratio of the extraction vessel, and ultrasound power. The extraction rate decreased with increasing particle size of samples, showing a maximun at a $CO_2$ flow rate of 6.2 L/min. Both the extraction rate and extraction yield increased with a decrease in the aspect ratio of the extraction vessel. For the ultrasoundassisted $SCCO_2$ extraction, the extraction yield was slightly increased when the $CO_2$ flow rate was below 6 mL/min with sample A and B.

• Korean Journal of Metals and Materials
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• v.46 no.4
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• pp.227-232
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• 2008

Solvent extraction reaction of cobalt by Alamine336 from strong hydrochloric acid solution was identified by analyzing the solvent extraction data reported in the literature. Analysis of the data by graphical method revealed that Alamine336 took part in the solvent extraction reaction as a monomer in the concentration ranges, [Co(II)] : 0.0169 - 0.102 M, [Alamine336] ; 0.02- 1.75 M, and [HCl ] : 5 - 10 M. The following solvent extraction reaction and equilibrium constant was obtained from the experimental data by considering the activity coefficients of chemical species present in the aqueous phase. $Co^{2+}+2Cl^{-}+R_3NHCl_{org}=CoCl_3\;R_3NH_{org}$, $K_{ex}=2.21$ The distribution coefficients of cobalt predicted in this study agreed well with those reported in the literature.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.447-453
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• 2004

Optimal conditions for the supercritical carbon dioxide $(scCO_{2})$ extraction of glycyr­rhizin from licorice (Glycyrrhiza glabra) were investigated, with an emphasis on the types and levels of modifiers. The morphology of the licorice tissue remaining after the $scCO_{2} $ extraction of glycyrrhizin was examined by scanning electron microscopy, coupled with measurements of ab­solute density. Conventional organic solvent extraction was also carried out for purpose of quantitative comparison. At 50 MPa and $60^{circ}C$ glycyrrhizin could not be extracted with pure $scCO_{2}$, while a considerable amount of glycyrrhizin was extracted when water was added to $scCO_{2}$ as a modifier. The highest recovery was found to be about $97\%$ when $70\%$ aqueous methanol was added to $scCO_{2}$ at a concentration of $15\%$. The optimal pressure and temperature for the supercritical fluid extraction of glycyrrhizin were observed to be 30 M Pa and $60^{circ}C$, respectively. Under these conditions, the percentage recovery of glycyrrhizin attained a maximum value of 102.67\pm$ $1.13\%$ within 60 min. Furthermore, in the case of $scCO_{2}$ modified with $70\%$ aqueous methanol, the licorice tissue obtained after extraction was found to be severely de­graded by excessive swelling, and the absolute density of the licorice residues was observed to be the highest.

• Archives of Pharmacal Research
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• v.23 no.2
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• pp.163-166
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• 2000

The effects of modifiers such as methanol, water diethylamine in methanol (10 v/v %), and diethylamine in water (10 v/v %) were investigated at three different concentrations (1, 5, and 10 v/v %) of the modifiers in supercritical $CO_2$ (SC-$CO_2$) in order to enhance the supercritical fluid extraction (SFE) efficiency of cephalotaxine from Cephalotaxus wilsoniana Leaves. Among the modifiers employed, methanol basified with diethylamine was found to greatly enhance the extraction efficiency relative to any other modifiers employed. The results suggest that cephalotaxine in plant matrices may be readily changed from SC-$CO_2$-insoluble salt to SC-$CO_2$-soluble free base by basified modifiers. In addition, SC-$CO_2$modified with basified methanol could enhance the extraction efficiency of cephalotaxine more than 30% when compared to the conventional organic solvent extraction.

• Bulletin of the Korean Chemical Society
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• v.29 no.7
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• pp.1327-1331
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• 2008

Novel $CO_2$-soluble 8-hydroxyquinoline (8-HQ) chelating agents were synthesized and evaluated for solubility and metal ion extraction ability in supercritical $CO_2\;(Sc-CO_2)$. Among them, secondary amide-containing 8- HQ derivatives cannot be dispersed well into Sc-$CO_2$, but tertiary amide-containing derivatives can dissolve completely in Sc-$CO_2$ even at low CO2 pressures, perhaps owing to the predominant intermolecular interaction between the chelating agent and the $CO_2$ molecule. Based on 8-HQ chelating agent solubility data, we investigated the extraction of metal ions ($Co^{2+}$, $Cu^{2+}$, $Sr^{2+}$, $Cd^{2+}$, and $Zn^{2+}$) using two highly $CO_2$-soluble 8-HQ derivatives (4d, 4e) in Sc-$CO_2$. The extraction efficiency of tertiary amide-containing 8-HQ ligands, both fluorinated and non-fluorinated forms, was dramatically increased in the presence of diethyl amine (organic base). We suggest that diethyl amine could play an important synergistic role in the stronger metal binding ability of 8-HQ through an in situ deprotonation reaction in Sc-$CO_2$ medium.