• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.233-239
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• 2003

We have developed a reliable and high-frequency genetic transformation and regeneration system via somatic embryogensis of Eleutherococcus sessiliflorus. Embryogenic callus obtained from seed were co- cultivated with Agrobacterium tumefaciens strain EHA101/pIG121Hm harboring genes for intron-$\beta$-glucoronidase(GUS), kanamycin and hygromycin resistance. Following co-cultivation, two types of samples(fine embrogenic calli and early globular embryo clusters) were cultivated on Murashige and Skoog(MS) medium containing 1 mg/L2.4-D for 3day in dark. Transient expression of GUS gene was found to be higher in the early globular embryo clusters than in the embryogenic calli. Also, co-cultivated period affected expression of GUS gene; the best result was obtained when globular embryo clusters were co-cultivated with Agrobacterium for 3 days. Subsequently, this callus transferred to selective MS medium containing 1mg/L2.4-D, 50mg/L kanamycin or/and 30mg/L hygromycin and 300mg/L cefortaxime. These embryogenic calls were subcultured to the same selection medium at every 2 weeks intervals. Approximately 24.5% of the early globular embryos co-cultivated with Agrobacterium for 3days produced kanamycin or/and hygromycin-resistant calli. Transgenic somatic embryos were converted into plantlets in half strength MS medium supplemented with 3mg/L GA$_3$ kanamycin and were confirmed by GUS histochemical assay and polymerase chain reaction analysis. Genomic Southem blot hybridization confirmed the incorporation of NPT II gene into the host genome.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.50-50
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• 2022

Recently, the use of mulching film has increased in soybean cultivation. Polyethylene (PE) films and biodegradable films (BF) have the advantages of improving soil moisture retention, geothermal maintenance, and CO2 maintenance as well providing weed control. Furthermore, BFs are a material that can compensate for the shortcomings of PE because it has the ability to decompose naturally by soil microorganisms, sunlight, and geothermal heat. Many researches have been carrying out studies regarding the development of BFs for these very reasons. This study was conducted better understand which films are optimal for soybean cultivation after evaluations of soybean growth and film characteristics of various BFs. BFs Farmsbio (Farm Hannong), Heulgro Film (Sejin Bio), Vonto Film (Eco-Hansung), two unnamed biodegradable films (Seojin Bio and Taesung), and a PE film were used in this study. For the control plots, no mulching was used. Experimental fields were fertilized according to conventional cultivation methods, tilled, and then covered with either BFs or PE films. After 1 week, soybean (cv. Daechan) seeds were seeded. Germination rate and plant height were measured at weekly intervals after seeding. In addition, pH, EC, and decomposition and light transmittance levels of films were measured during the experimental period. Daily average temperatures and relative humidity in soils was measured during the experimental period. There was no significant difference in germination rates and plant height in both crops grown with BFs and PE films, but crops grown in the control plot had significantly lower germination rates and growth. Soil pH was not significantly different regardless of treatments (BF, PE, and non-mulching) at 14, 28, and 42 days after seeding. In general, the EC contents in the control plots was lower than in crops grown using BFs and PE films. With the exception of some BFs, light transmittance and decomposition levels of films did not, in general, increase up to 70 days after soybean seeding. Since this study is ongoing, we are continually investigating these parameters. The average daily moisture in soil was higher in crops grown with BFs and PE films than in the control plot. However, the daily average soil temperature was not consistent regardless of treatments. Therefore, the BFs used in this study can be used without negative impacts on soybean growth.

• The Korean Journal of Pesticide Science
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• v.3 no.1
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• pp.66-77
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• 1999

In order to elucidate the fate of the residues of the bipyridylium herbicide paraquat in soil, maize plants were grown for 4 weeks on the specially-made pots filled with two different types of soils containing fresh and 6-week-aged residues of [$^{14}C$]paraquat, respectively. The mineralization of [$^{14}C$]paraquat to $^{14}CO_{2}$ during the aging period and the cultivation period of maize plants amounted to $0.13{\sim}0.18%$ and $0.02{\sim}0.17%$, respectively, of the original $^{14}C$ activities. At harvest the roots and shoots contained less than 0.1% and 0.01% of the originally applied $^{14}C$ activities, respectively, whereas the $^{14}C$ activities remaining in soil were more than 97% in both soils. The water extractability of the soil where maize plants were grown for 4 weeks was less than 1.2% of the original $^{14}C$ activities. Most of the non-extractable soil-bound residues of [$^{14}C$]paraquat were incorporated into the humin fraction. Soil pHs during the aging of soil B and after cultivation in all treatments increased. The distribution of the $^{14}C$ activities in subcellular particles of the maize plant roots was the highest in the residue fraction(incompletely homogenized tissue). Dehydrogenase activities increased after vegetation, regardless of soil aging.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.785-789
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• 2021

Paprika productivity is different even in the same quality greenhouse and in the same region. These differences are known to due to differences in various environmental factors. This study was conducted to investigate the difference in the level of various environmental factors between high-productivity (HPF) and low-productivity (LPF) greenhouses. The largest difference between the two greenhouses in the daily or weekly average values of major environmental factors was the CO₂ concentration, but the LPF was higher than the HPF, so it was not determined as a factor for the difference in productivity. Correlation analysis among 14 environmental factors showed a high correlation among irradiation or related factors in moisture. The regression coefficients of the linear regression model between vapor pressure deficit and relative humidity were -0.0202kpa in HPF and -0.0262kpa in LPF. In particular, in February and March, the vapor pressure deficit in LPF was 1.5kpa or more, and the cumulative vapor pressure deficit compared to the cumulative irradiation at the early period of cultivation increased rapidly. The reason for the low productivity in LPF is thought to be that the plant was affected by moisture stress due to high vapor pressure deficit and transpiration under low irradiation conditions in the early period of cultivation and in winter.

• Applied Chemistry for Engineering
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• v.28 no.3
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• pp.306-312
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• 2017

Changes in the cell growth and lipid accumulation of marine microalga Dunaliella tertiolecta were investigated in response to the combination of different stress factors including the variation of iron supply as a primary stress factor and different options in light irradiation and $CO_2$ supply as a secondary stress factor. High or limited Fe conditions could act as a stress for lipid synthesis. As a secondary stress factor, non-$CO_2$ condition was good for lipid accumulation, but the overall cell growth was sacrificed significantly after a long-time cultivation. Dark condition as a secondary stress factor also favored lipid accumulation and the extent of cell density reduction at the early period in the dark was small compared to other stress conditions. The two-stage cultivation strategy was necessary to maximize lipid production because tendencies of the cell growth and lipid content were not identical under the chosen stress condition. The first stage was for preparing a high cell density under the normal growth-favoring condition and the second stage was the stress condition to induce lipid accumulation in a short time. The short-term (12 h) incubation under the 5X Fe (3.25 mg/L) and dark conditions resulted in the best lipid productivity of 1.44 g/L/d providing 2 g/L inoculum at the second stage.

• Korean Journal of Microbiology
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• v.44 no.4
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• pp.352-357
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• 2008

We evaluated cultivation methods to obtain pure cultures of previously uncultivated bacteria from soil. Soil bacteria (suspensions) were inoculated onto various oligotrophic media with one of the following additives: 1) soil extract; 2) anthraquinone disulfonate (humic acid analogue); 3) acyl homoserine lactones (quorum-signaling compounds); 4) catalase (for the protection of bacteria from exogenous peroxides). After the relatively long period (60 days) of incubation with elevated concentrations of $CO_2$ (5%, v/v), the media containing catalase showed the highest colony count. We purified 147 randomly selected colonies from the media and the isolates were subjected to the phylogenetic analyses of their 16S rRNA gene sequences. Phylogenetic analysis revealed that approximately 30% of the isolates might belong to novel species or novel family, suggesting that the media and incubation conditions used could be useful for the cultivation of as-yet-uncultured bacteria. Especially, bacteria belonging to the phylum Acidobacteria, ubiquitous bacterial taxon known as an uncultured bacterial group (at least difficult to culture from environmental samples), were successfully cultured in this study.

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.128-133
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• 2015

Mushrooms can grow in nature when adequate temperature and humidity are maintained, but such condition can be satisfied for only a short period of time on an annual continuum. Therefore, it can be deemed that a majority of the distributed mushrooms in the current market are produced in an artificially manipulated environment. This study was conducted to resolve the problem of the Oyster mushroom cultivation house's internal temperature and humidity imbalance, where the Oyster mushrooms are cultivated in a multi-layered shelves. The air circulation fans were installed to improve the air uniformity of the Oyster mushroom cultivation house by using multi-layered shelves. During the experiment, the ambient temperature and the ambient relative humidity ranged from $5.2^{\circ}C$ to $20.4^{\circ}C$ and 40% to 100% respectively. Due to the change of the outdoor temperature, the internal temperature of the Oyster mushroom cultivation house also changed, ranging from $13.3^{\circ}C$ to $18.4^{\circ}C$, but the temperature gap between the different internal location of the facility during the conforming recorded time only ranged from $0.2^{\circ}C$ to $1.3^{\circ}C$, being significantly stable. The internal relative humidity, ranging from 82% to 96%, also changed due to the change of the outdoor temperature. Nevertheless, the relative humidity gap between the different internal location during the conforming recorded time only ranged from 2% to 7%. Furthermore, the research staff were able to maintain the concentration of $CO_2$ from 575ppm to 731ppm(below 1,000ppm was the goal) indicating the possibility of an even management of the internal environment by installing the air circulation fan.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.629-641
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• 2022

This research was conducted to secure fundamental data for development of a recirculating hydroponic system. To achieve this, Lycopersicum esculentum var. 'Dafnis' and 'TY Tiny' were grown with Yamazaki hydroponic solution and the inorganic element concentrations of plant leaves and nutrient solution of supplied and drained were analyzed periodically. The T-N and P contents in both varieties of tomato leaves showed gradual decreasing tendencies with the passage of time. The 'TY Tiny' tomato had higher contents of those two nutrients than 'Dafnis' tomato in the late stage. The K content of 'Dafnis' tomato was high in the early growth stage, but low in the late stage. However, that of 'TY Tiny' tomatoes rose in the late stage. The Ca content gradually increased in both varieties of tomato in the latter stages. The EC of the drained nutrient solution in both varieties of tomato showed increasing tendencies as time had passed, but the pH was get lowered in the drained solution. The concentrations of NO₃-N, K, Ca, Mg, Na, Fe, and B, except PO₄-P in the drained nutrient solution were generally higher than those in the supplied solution, especially in the period of October through December. The above results can be used for controlling of nutrient concentrations in the recirculated hydroponic cultivation of tomato.

• Korean Journal of Environmental Agriculture
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• v.30 no.1
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• pp.24-30
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• 2011

BACKGROUND: High input to the arable land is contributed to increasing productivity with causing the global environmental problems at the same time. Rapeseed cultivation has been forced to reassess its positive point for utilization of winter fallow field. The Objective of this study was performed to assess the environmental impact of rapeseed cultivation with double-cropping system in paddy rice on Yeonggwang district using life cycle assessment technique. METHODS AND RESULTS: For assessing each stage of rapeseed cultivation, it was collected raw data for input materials as fertilizer and pesticide and energy consumption rate by analyzing the type of agricultural machinery and working hours by 1 ton rapeseed as functional unit. Environmental impacts were evaluated by using Eco-indicator 95 method for 8 impact categories. It was estimated that 216 kg $CO_2$-eq. for greenhouse gas, 3.98E-05 kg CFC-11-eq. for ozone lazer depletion, 1.78 kg SO2-eq. for acidification, 0.28 kg $PO_4$-eq. for eutrophication, 5.23E-03 kg Pb-eq. for heavy metals, 2.51E-05 kg B(a)p-eq. for carcinogens, 1.24 kg SPM-eq. for smog and 6,460 MJ LHV for energy resource are potentially emitted to produce 1 ton rapeseed during its whole cultivation period, respectively. It was considered that 90% of these potential came from chemical fertilizer. For the sensitivity analysis, by increasing the productivity of rapeseed by 1 ton per ha, potential environmental loading was reduced at 22%. CONCLUSION(s): Fertilization affected most dominantly to the environmental burden, originated from the preuse stage, i.e. fertilizer manufacturing and transporting. It should be included and assessed an indirect emission, which is not directly emitted from agricultural activities. Recycling resource in agriculture with reducing chemical fertilizer and breeding the high productive variety might be contribute to reduce the environmental loading for the rapeseed cultivation.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.848-852
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• 2012

Vegetation can make not only to lower the urban ambient air temperature (UAAT) by crop evapotranspiration (ET) and increasing solar radiation albedo, but also to reduce the urban air pollution by $CO_2$ uptake and $O_2$ emission in addition to the reducing ozone concentrations by aid of lower the UAAT. To evaluate the effect of vegetation on urban heat island mitigation (UHIM), the climate change of 6 cities during 30 years are analysed, and the amount of ET, $CO_2$ uptake, $O_2$ emission and ozone concentrations are estimated in Korea. The most hot season is the last part of July and the first part of August, and the highest average UAAT of a period of ten days was $35.03^{\circ}C$ during 30 years (1979 - 2008). The mean values of maximum ET of rice and soybean in urban area during urban heat island phenomena were 6.86 and $6.00mm\;day^{-1}$, respectively. The effect of rice and soybean cultivation on lowering the UAAT was assessed to be 10.5 and $3.0^{\circ}C$ in Suwon, respectively, whereas the differences between the UAAT and canopy temperature at urban paddy and upland in Ansung were 2.6 and $2.2^{\circ}C$. On the other hand, the urban-garden in Suwon city had resulted in lowering the UAAT and the surface temperature of buildings to 2.0 and $14.5^{\circ}C$, respectively. Furthermore, the amounts of $CO_2$ uptake by rice and soybean were estimated to be 20.27 and $15.54kg\;CO_2\;10a^{-1}day^{-1}$, respectively. The amounts of $O_2$ emission by rice and soybean were also assessed to be 14.74 and $11.30kg\;O_2\;10a^{-1}day^{-1}$, respectively. As other cleaning effect of air pollution, the ozone concentrations could be also estimated to reduce 21.0, 8.8, and 4.0 ppb through rice-, soybean cultivation, and urban gardening during most highest temperature period in summer, respectively.