• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.51-54
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• 1998

It has been proved that co-cultivation of human neroblastoma cells and human fibroblast cells can enhance nerve cell growth and the production of BDNF in perfusion cultivation. In batch co-cultivation, maximum cell density was increased up to 1.76${\times}$106 viable cells/mL from 9${\times}$105 viable cells/mL of only neuroblastoma cell culture. The growth of neuroblastoma cells was greatly improved by culturing both nerve and fibroblast cells in a perfusion process, maintaining 1.5${\times}$106 viable cells/mL, which was much higher than that form fed-batch cultivation. The nerve cell growth was greatly enhance in both fed-batch and perfusion cultivations while the growth of fibroblast cells was not. It strongly implies that the factors secreted from human fibrobast cells and/or the environments of co-culture system can enhance both cell growth and BDNF secretion. Specific BDNF production rate was not enhanced in co-cultures; however, the production period was increased as the cell growth was lengthened in the co-culture case. Competitive growth between nerve cells and fibroblast cells was not observed in all cases, showing no changes of fibroblast cell growth and only enhancement of the neuroblastoma cell growth and overall BDNF production. It was also found that the perfusion cultivation was the most appropriate process for cultivating two cell lines simultaneously in a bioreactor.

• Korean Journal of Environmental Agriculture
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• v.42 no.4
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• pp.435-443
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• 2023

Global concern over climate change, driven by greenhouse gas emissions, has prompted widespread interest in sustainable solutions. In the agricultural sector, biochar has emerged as a focal point for mitigating these emissions. This study investigated the impact of continuous biochar application on CO₂ and N₂O emissions during the spring cabbage cultivation period. Greenhouse gas emissions in the biochar treatment groups (soils treated with 1, 3, and 5 tons/ha of rice husk biochar) were compared to those in the control group without biochar. During the spring cabbage cultivation period in 2022, the total CO₂ emissions were in the range of 71.6-119.0 g/m² day, and in 2023, with continuous biochar application, they were in the range of 71.6-102.1 g/m² day. The total emissions of N₂O in 2022 and 2023 were in the range of 11.7-23.7 and 7.8-19.9 g/m² day, respectively. Overall, greenhouse gas emissions decreased after biochar treatment, confirming the positive influence of biochar on mitigating greenhouse gas release from the soil. Nevertheless, further research over an extended period exceeding five years is deemed essential to delve into the specific mechanisms behind these observed changes and to assess the long-term sustainability of biochar's impact on greenhouse gas dynamics in agricultural settings.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.26 no.2
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• pp.105-112
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• 2020

As sprout vegetables of interest growing, its maintaining the quality of the technology was needed to solve the problem of increasing growth and maintain quality after harvest. This experiment proved that the quality of radish sprout vegetable was affected by CO₂ treatment during cultivation. Thus, the effect of CO₂ treatment during cultivation on postharvest quality of radish sprout vegetable was investigated in terms of the quality changes in weight loss, gas partial pressure, SPAD, hue angle external appearance during storage at polypropylene film (thickness 30 ㎛) at 10℃. CO₂ treatment used the way to gas with 700 ppm or carbonated water with 700 ppm and 1,400 ppm. The study revealed that growths on CO₂ treated plant were more than those of non-treatment on stem length. After harvesting, the CO₂ treated plant and control growing little different characteristics on fresh weight, plant length and so on. However, there were no differences between the CO₂ treated plant and control on the Fv/Fm and SOD (superoxide dismutase). In gas partial pressure, the O₂ consumption and CO₂ accumulation of the CO₂ treated plant tended to be more than that of non-treated plant. This study also checked that after packaging, the effects of CO₂ treatment during cultivation on the quality of radish sprout vegetable was not significant. However, there were tended to CO₂ treatments were lower value compared to control on SPAD, hue angle and general appearance. CO₂ treatments of radish sprouting vegetable before harvest were improve growth of stem length, but ones were not improving the maintain of quality on radish sprout vegetable during shelf-life period. The results indicated that CO₂ treatment only affected stem elongation until radish sprout vegetable its growth.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.36-40
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• 2008

Studies were processed to confirm the differences of productivity according to sawdust size and effect of additives for sawdust cultivation of shiitake. In results of investigation until shiitake mycelia fully spread on surface of sawdust medium, mycelial growth in treatment of sawdust size $3{\sim}5\;mm$ with $CaCO_3$ and $KNO_3$, and treatment of sawdust sizw $3{\sim}5\;mm$ without $CaCO_3$ and $KNO_3$ was the fastest. And, in investigation of rate of weight reduction, treatment of sawdust size below 1 mm without $CaCO_3$ and $KNO_3$ was the highest. The tendency of fruiting was somewhat different according to treatments. In case of sawdust size below 1 mm with $CaCO_3$ and $KNO_3$, yield produced from 1st to 2nd flushing period was over 80%. However, in case of sawdust size below 1 mm plus $5{\sim}7\;mm$ without $CaCO_3$ and $KNO_3$, yield produced from 3rd to 4th flushing period was more than it from 1st to 2nd flushing period and rate of yield produced from 1st to 2nd flushing period to total yield was 34%. Results of investigation on productivity, yield and the number of fruit-bodies were remarkably different according to treatments. Sawdust size $3{\sim}5\;mm$ with $CaCO_3$ and $KNO_3$, whose total yield and the number of fruit-bodies were 568 g and 67, respectively, was the highest. And, total yield of medium of sawdust size below 1 mm plus $2{\sim}3\;mm$ without $CaCO_3$ and $KNO_3$ was lowest as 227 g and the number of fruit-bodies of medium of sawdust size below 1 mm plus $5{\sim}7\;mm$ without $CaCO_3$ and $KNO_3$ was lowest as 24. In investigation on amount of fruit-bodies over 10 g, medium of sawdust size $3{\sim}5\;mm$ with $CaCO_3$ and $KNO_3$ was highest as 397 g.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2003.10a
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• pp.347-350
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• 2003

Pleurotus eryngii is one of the most promising mushrooms produced on the domestic farms. The quality as well as quantity of Eryngii is sensitively affected by micro climate factors such as temperature, relative humidity, $CO_2$ concentration, and light intensity. At the commercial mushroom cultivation houses, this study was carried out to find out reasonable range of each environmental factor and yield together with economic and safe structures influencing on the optimal productivity of Eryngii. This experiment was conducted from Jan. 26, 2003 to Aug. 2, 2003. Ambient temperature during the experiment period was not predominantly different from that of a normal year. The capacity of the hot water boiler and the piping systems were not enough. Maximum temperature difference between the upper and lower growth stage during a heating time zone was about $6^{\circ}C$. The max. and min. relative humidity were ranged approximately $42{\sim}100%$. The $CO_2$ concentration and the illuminance were lowly maintained during growing period. The average yield per bottle was about $54{\sim}102g$.

• Journal of Mushroom
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• v.16 no.2
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• pp.79-85
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• 2018

This study was carried out to separately assess the production of post-culture, germinating, growing, and resting stages of Lentinula edodes grown in a smart cultivation facility based on ICT technology. The cultivation environment of the greenhouse facility was controlled to remain at different set points throughout a range of high ambient temperatures. Production of Sanjo-701-ho, which is a typical summer cultivar, and the new cultivar Sanjo-701-ho were tested in this ICT-based smart cultivation system. Sanjo-701-ho produced 353.7 g/bag of mushrooms from June to October, while Sanjo-701-ho produced 270.4 g/bag. These production amounts were stable over the high-temperature range of $20-35^{\circ}C$.

• Korean Journal of Environmental Agriculture
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• v.31 no.3
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• pp.271-276
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• 2012

BACKGROUND: It is hard to control the agricultural products exceeding MRL (maximum residue limit) before forwarding. Therefore, NAQS (National Agricultural Products Quality Management Service, South Korea) established PHRL (pre-harvest residue limit) on agricultural products during their cultivation periods. This study was performed to set the PHRLs of peach during cultivation period, and also to estimate biological half-lives for residues of clothianidin and fluquinconazole. METHODS AND RESULTS: Two groups of peach were treated under Korean GAP (Good Agricultural Practices) with application time, single and triple treatments. Sample was collected over 14days (each after 0, 2, 4, 6, 8, 10, 12, 14 days. 8times), and clothianidin and fluquinconazole were analyzed by HPLC/DAD and GC/ECD, respectively. CONCLUSION: The biological half-life of clothianidin in single treatment and triple treatment was 5.2days and 7.0days. That of fluquinconazole was also 3.9days and 4.1days, respectively. The PHRL of peach on 10days before harvest was 1.4 mg/kg in clothianidin and 1.8 mg/kg in fluquinconazole.

• Korean Journal of Medicinal Crop Science
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• v.15 no.2
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• pp.100-104
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• 2007

A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumefaciens-mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; $OD_{600}$ = 0.8), co-cultivation period (3 days), and acetosyringone concentration $(100\;{\mu}M)$ had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level(30 mg/l). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.200-206
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• 2003

Pleurotus eryngii(King oyster) is one of the most promising mushrooms produced on the domestic farms. The quality as well as quantity of Eryngii is sensitively affected by micro climate factors such as temperature, relative humidity, $CO_2$ concentration, and light intensity. To safely produce high-quality Eryngii all the year round, it is required that the environmental factors be carefully controlled by well designed structures equipped with various facilities and control systems. At the commercial mushroom cultivation house(A,B), this study was carried out to find out reasonable range of each environmental factor and yield together with economic and safe structures influencing on the optimal productivity of Eryngii. this experiment was conducted for about two-month from Nov. 11, 2002 to Dec. 30, 2002 in Eryngii. cultivation house-A, B. Ambient temperature during the experiment period was not predominantly different from that of a normal year. The capacity of the hot water boiler and the piping systems were not enough. Maximum air temperature difference between the upper and lower growth stage during a heating time zone was about 2~3$^{\circ}C$. The max. and min. relative humidity were ranged approximately 60~100%, and average relative humidity was ranged approximately 80~100%. And $CO_2$concentration increased until maximum 1,600~1,800 ppm with the passing growing period. The illuminance in cultivation house was widely distributed from 20lx to 160 lx in accordance with position, and it was maintained lower than the recommended illuminance range 100~200 lx. The average yield per bottle was about 67~85g. But the optimal productivity will be evaluated by considering the quality and quantity of mushroom production, energy requirements, facility construction and management cost, etc.

• Journal of Mushroom
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• v.18 no.4
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• pp.331-338
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• 2020

This study was conducted to cultivate new Lepista nuda varieties with shorter cultivation period and better fruiting body compared to that of wild strains, for mass production and commercial application. Eighteen genetic resources of L. nuda were collected and grown in boxes using rice straw-fermented growth medium. Four lines with fruiting bodies were formed and selected as cross-breeding lines. Although 657 combinations were crossed through monospore crossing, only 17 combinations were bred between the 'CBMLN-19' line and the 'CBMLN-30' line. Among them, 8 lines with fast mycelial growth and high density were selected. After inoculating the rice straw-fermented growth medium with 14 genetic resources and 8 cross-breeding lines, their incubation period was investigated. Six of the cross-breeding lines completed their incubation in 20 days, while 7 of the 14 genetic resources took more than 40 days to complete their incubation, reducing the incubation period by more than 20 days in most cross-breeding lines. After the incubations were completed, the clay loam soil was covered with for post-cultivation, and when the mycelial cultivation was complete, the formation of fruiting bodies was induced after scraping the mycelial bodies under these environmental conditions: 14℃, 95% relative humidity or higher, and 1,500 to 2,000 ppm CO2 concentration. The temperature was reduced to 6℃ at night, resulting in a low temperature shock. Thus, 4 lines of fruiting bodies occurred from two genetic resources 'CBMLN-31' and 'CBMLN-44' and two cross-bred lines 'CBMLN-96' and 'CBMLN-103'. After inoculation, the longest period for fruiting bodies to occur was 100 days for the control:, the genetic resource 'CBMLN-31', and the shortest period (45 days) was observed for the cross-breeding line 'CBMLN-103'. The result of the investigation of the fruiting body characteristics shows that the cross-bred line 'CBMLN-103' showed a small form with 1.9 g of individual weight and 123validstipes per box, which was the highest incidence among the four lines. Another cross-bred line, 'CBMLN-96', had an individual weight of 5.5 g, which is larger than that of 'CBMLN-103'; however, the number of valid stipes per box was 30 less than that of 'CBMLN-103'. Quantity analysis showed that the control, 'CBMLN-31', had the highest quantity of 783 g per box, followed by the cross-bred line, 'CBMLN-96' with 165 g per box, and then the 'CBMLN-103' with 232 g. The quantity of the two crossbred lines was lower than that of the control 'CBMLN-31'; however, the amount of fruiting bodies was higher, and the cultivation period was shortened by 32 to 33 days. Therefore, these two lines would be selected as superior lines.