• Journal of Nutrition and Health
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• v.39 no.4
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• pp.323-330
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• 2006

Peripheral insulin resistance in obese/type II diabetes animals results from an impairment of insulin-stimulated glucose uptake into skeletal muscle. Insulin stimulate the translocation of GLUT4 from intracellular location to the plasma membrane. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) is implicated in mediation of fusion of GLUT4-containing vesicle with the plasma membrane. Present study investigated regulatory effects of Rhodiola sachalinensis administration and exercise training on the expression of GLUT4 protein and SNAREs protein in skeletal muscles of obese Zucker rats. Experimental animals were randomly assigned into one of five groups ; lean control(LN), obese control(OB), exercise-treated(EXE), Rhodiola sachalinensis-treated(Rho), combine of Rho & EXE (Rho-EXE). All animals of exercise training (EXE, Rho-EXE) performed treadmill running for 8 weeks, and animals of Rho groups (Rho, Rho-EXE) were dosed daily by gastric gavage during the same period. After experiment, blood were taken for analyses of glucose, insulin, and lipids levels. Mitochondrial oxidative enzyme (citrate synthase, CS ; $\beta$-hydroxyacyl-CoA dehydrogenase, $\beta$-HAD) activity were analysed. Skeletal muscles were dissected out for analyses of proteins (GLUT4, VAMP2, syntaxin4, SNAP23). Results are as follows. Exercise and/or Rhodiola sachalinensis administration significantly reduced body weight and improved blood lipids (TG, FFA), and increased insulin sensitivity. Endurance exercise significantly increased the activity of mitochondrial enzymes and the expression of GLUT4 protein, however, administration of Rhodiola sachalinensis did not affect them. The effect of exercise and/or Rhodiola sachalinensis administration on the expression of SNARE proteins was unclear. Our study suggested that improvement insulin sensitivity by exercise and/or Rhodiola sachalinensis administration in obese Zucker rats is independent of expression of SNARE proteins.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.1
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• pp.23-33
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• 2018

Cushing's syndrome (CS) is a collection of symptoms caused by prolonged exposure to excess cortisol. Chronically elevated glucocorticoid (GC) levels contribute to hepatic steatosis. We hypothesized that histone deacetylase inhibitors (HDACi) could attenuate hepatic steatosis through glucocorticoid receptor (GR) acetylation in experimental CS. To induce CS, we administered adrenocorticotropic hormone (ACTH; 40 ng/kg/day) to Sprague-Dawley rats by subcutaneous infusion with osmotic mini-pumps. We administered the HDACi, sodium valproate (VPA; 0.71% w/v), in the drinking water. Treatment with the HDACi decreased steatosis and the expression of lipogenic genes in the livers of CS rats. The enrichment of GR at the promoters of the lipogenic genes, such as acetyl-CoA carboxylase (Acc), fatty acid synthase (Fasn), and sterol regulatory element binding protein 1c (Srebp1c), was markedly decreased by VPA. Pan-HDACi and an HDAC class I-specific inhibitor, but not an HDAC class II a-specific inhibitor, attenuated dexamethasone (DEX)-induced lipogenesis in HepG2 cells. The transcriptional activity of Fasn was decreased by pretreatment with VPA. In addition, pretreatment with VPA decreased DEX-induced binding of GR to the glucocorticoid response element (GRE). Treatment with VPA increased the acetylation of GR in ACTH-infused rats and DEX-induced HepG2 cells. Taken together, these results indicate that HDAC inhibition attenuates hepatic steatosis through GR acetylation in experimental CS.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.307.2-308
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• 2002

Acharan sulfate. a new glycosaminoglycan(GAG) isolated from the giant African snail Achatina fulica. was shown to have antitumor activity in vivo. To elucidate the mechanisms for the antitumor activity. we examined its impact on professional antigen presenting cells such as macrophages and dendritic cells (DCs). Acharan sulfate stimulated cytokine production (TNF-a and IL -1b). nitric oxide release. and morphological changes in a dose dependent manner on a macrophage cell Line Raw 264.7 cells. The differentiation-inducing activity of acharan sulfate was examined on immature DCs. Immature DCs were generated from mouse bone marrow (BM) cells by culturing with GM-CSF and IL-4, and then stimulated with acharan sulfate. The resultant DCs were then examined for funcional and phenotypic properties. It was found that acharan sulfate could induce functional maturation of immature DCs as determined by increased allogenic mixed lymphocyte reaction (MLR) and IL-12 production. Phenotypic. analysis for the expression of class II MHC molecules and major co-stimulatory molecules such as B7-1, B7-2 and CD40 also confirmed that acharan sulfate could induce maturation of immature DCs. These results suggest that that the antitumor activity of acharan sulfate is at least in part due to activation adn induction of differentiation of professinal antigen presenting cells. (omitted)

• Nuclear Engineering and Technology
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• v.1 no.1
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• pp.23-31
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• 1969

Whole cure shrimp, eviscerated common squid and little squid meats were irradiated with gamma radiation from 60Co using varying dose up to one Mrad. Changes in quality were assessed by organoleptic, chemical (VRS and TMA-N), biochemical (hypoxanthine) and microbiological tests； and the effect of radiation on prolonging of the storage life at 2-4 C was studied. With the cure shrimp, 0.12-0.20 Mrad appears to be optimal with 8 days of storage life, whereas the unirradiated control samples had a storage life of 3 days. With the common squid meat, 0.20-0.30 Mrad appears to be optimal with 14-21 days of storage life, where-as the unirradiated control samples had a storage life of 8 days. And for the little squid meat, 0.12-0.20 Mrad appears to be optimal with 10 days of storage, where-as the unirradiated control samples had a storage life of 5 to 6 days.

• Journal of Korean Society of Water and Wastewater
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• v.11 no.2
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• pp.112-127
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• 1997

The purpose of this study is to get optimum operating factors of Upflow Anaerobic Sludge Blanket (UASB) reactor by introducing methods that make it to reduce inhibition possible in each process wastewater treatment. The used substrates, concentrated corn starch liquid (CSL) wastewater, modified starch, filtering and decoloring wastewater, ion refining wastewater, and mixed wastewater including modified starch and not including modified starch, are generated from molasses process. The seeding sludge is the digested sludge that had been applied to molasses wastewater. Batch test to reduce the inhibition factors that might be existed in each wastewater was examined. Based on the this test, the optimum operating factors according to alkalinity and pH variation was studied through the continuous test using three 5.5 L UASB reactor. The first reactor added $NaHCO_3$ to control alkalinity. The hydraulic retention time (HRT) reduced to 8 hours and the organic loading rate increased gradually. The second reactor changed the pH of influent from 7.0 to 6.0 using NaOH. The third reactor was operated without changes to compare the above two reactors. As the result, the inhibition in concentrated CSL wastewater was removed by adding iron (II). When trace metals were added to mixed wastewater not including modified starch, the digestability by gas production rate increased to more fifty percentage than mixed wastewater that was not adding the trace metals. The reason that the inhibition did not decreased in spite of adding trace metals and nutrients was influenced by high concentration generated during the acid fermentation. The UASB reactors using the mixed wastewater with the most effective performance were operated as 500 mg/L as $CaCO_3$ alkalinity and 6.0 pH at steady state, and at this time, the gas production rates were 283 and 311mL gas/g $COD_{added}$. The COD removal rates were 84.7 and 86.3%, respectively.

• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.705-713
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• 2019

This study was conducted to determine the effects of dietary non-genetically modified organism (non-GMO) wheat-based diets on the growth performance, nutrient digestibility, blood profile and meat quality of grower-finisher pigs. A total of 70 [(Landrace × Yorkshire) × Duroc] growing pigs with an initial body weight of 26.15 ± 1.57 kg were used in a 112 day trial. The dietary treatments were as follows: (I) CD, corn-based diet and (II) non-GMO WD, a non-genetically modified organism wheat-based diet. Each treatment consisted of 7 replicate pens with 5 pigs per pen. In the current study, the pigs fed the corn-based diet had a higher body weight than the pigs fed the non-GMO wheat-based diet at day 21 and day 77 (p < 0.05). There was a significant difference in the average daily gain (ADG) during the first 21 days (p < 0.05). The non-GMO wheat-based diet had no effect on nutrient digestibility. In addition, the non-GMO wheat-based diet had no effect on the blood profile except for blood urea nitrogen (BUN) at d 21. In conclusion, the non-GMO wheat-based diet only had a slight effect on the growth performance of growing pigs but had no significant impact on the nutrient digestibility, fecal score, blood profile and meat quality of the pigs during the grower-finisher period.

• The Journal of Advanced Prosthodontics
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• v.7 no.1
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• pp.27-31
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• 2015

PURPOSE. The purpose of this report was to evaluate the effect of the fabrication method and material type on the fracture strength of provisional crowns. MATERIALS AND METHODS. A master model with one crown (maxillary left second premolar) was manufactured from Cr-Co alloy. The master model was scanned, and the data set was transferred to a CAD/CAM unit (Yenamak D50, Yenadent Ltd, Istanbul, Turkey) for the Cercon Base group. For the other groups, temporary crowns were produced by direct fabrication methods (Imident, Temdent, Structur Premium, Takilon, Systemp c&b II, and Acrytemp). The specimens were subjected to water storage at $37^{\circ}C$ for 24 hours, and then they were thermocycled (TC, $5000{\times}$, $5-55^{\circ}C$) (n=10). The maximum force at fracture (Fmax) was measured in a universal test machine at 1 mm/min. Data was analyzed by non-parametric statistics (${\alpha}$=.05). RESULTS. Fmax values varied between 711.09-1392.1 N. In the PMMA groups, Takilon showed the lowest values (711.09 N), and Cercon Base showed the highest values (959.59 N). In the composite groups, Structur Premium showed the highest values (1392.1 N), and Acrytemp showed the lowest values (910.05 N). The composite groups showed significantly higher values than the PMMA groups (P=.01). CONCLUSION. Composite-based materials showed significantly higher fracture strengths than PMMA-based materials. The CADCAM technique offers more advantages than the direct technique.

• The Journal of Advanced Prosthodontics
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• v.3 no.4
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• pp.186-189
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• 2011

PURPOSE. Porcelain fused to metal (PFM) crowns provide the best treatment option for teeth that have a large or defective restoration. More than 20% of teeth with PFM crowns or bridges require non-surgical root canal treatment (NSRCT). This may be due to the effect of restorative procedures and the possible leakage of bacteria and or their by-products, which leads to the demise of the tooth pulp. Thus, this study was planned to compare the ability of the restorative materials to seal perforated PFM specimens. MATERIALS AND METHODS. The study evaluates the ability of amalgam, composite or compomer restorative materials to close perforated PFM specimen's in-vitro. Ninety PFM specimens were constructed using Ni-Cr alloys and feldspathic porcelain, and then they were divided into 3 groups: amalgam (A), composite + Exite adhesive bond (B) and compomer + Syntac adhesive bond (C). All the PFM samples were embedded in an acrylic block to provide complete sealing of the hole from the bottom side. After the aging period, each group was further divided into 3 equal subgroups according to the thermocycling period (one week for 70 cycles, one month for 300 cycles and three months for 900 cycles). Each subgroup was put into containers containing dye (Pelikan INK), one maintained at $5^{\circ}C$ and the other at $55^{\circ}C$, each cycle for 30 sec time. The data obtained was analyzed by SPSS, 2006 using one way ANOVA test and student t-test and significant difference level at (P<.01). RESULTS. The depth of dye penetration was measured at the interfaces of PFM and filling materials using Co-ordinate Vernier Microscope. The lowest levels of the dye penetration for the three groups, as well as subgroups were during the first week. The values of dye leakage had significantly increased by time intervals in subgroups A and C. CONCLUSION. It was seen that amalgam showed higher leakage than composite while compomer showed the lowest level of leakage.

• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.4
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• pp.710-716
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• 1998

It is one of difficulties to control children who show definitely negative behavior in dental clinic. In such a case, the pharmacologic management has been used to provide quality care, minimize the extremes of disruptive behavior, promote a positive psychologic response to treatment and patient welfare and safety. Deep sedation can be defined as a controlled, pharmacologically-induced state of depressed consciousness from which the patient is not easily aroused which may be accompanied by a partial loss of protective reflexes. In this retrospective report, the sedation records of 200 pediatric dental patients of ASA Class I & II who were not successfully treated under conscious sedation were used for analysis. Most frequently used regimen of deep sedation was the co-medication of midazolam(0.3mg/kg), enflurane(1.0-2.0 vol%) and 50-70% $N_2O_2$. The average age and weight of the patients was 4.6 yr (S.D: 2.72) and 18.7kg(S.D: 6.35) respectively. The average operative time was 52 minutes and midazolam (0.1-0.2cc) was additionally administered intranasally to prolong the operative time as needed. The episodes of untoward side effects were reported during and/or after the procedure in 58 patients. Serious adverse reactions such as cyanosis or laryngospasm were even reported in 7 patients but without mortality. Deep sedation is a very effective way of completing the dental treatments for those who failed to respond well to the conscious sedation. This technique has many practical advantages over general anesthesia case but the demands for the rigid monitoring criteria limit its use in general practice setting. The continuous efforts to improve the safety of the medication and the technique are required for the benefits of the patients and parent.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.2
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• pp.55.5-56
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• 2016

Turbulence plays an important role in molecular clouds. However, the properties of turbulence are poorly understood. In order to study the influence of turbulence in molecular clouds, we need to sample the turbulent properties in the full range of scales down to sonic scale. We mapped the $20^{\prime}{\times}60^{\prime}$ area covering the Orion Molecular Cloud (OMC) 1-4 region in HCN 1-0 and HCO+ 1-0 with Taeduk Radio Astronomy Observatory (TRAO) 14-m telescope as part of the TRAO key science program, "Mapping turbulent properties of star-forming molecular clouds down to the sonic scale (PI: Jeong-Eun Lee)". In addition, we combine our TRAO data with other molecular line maps ($^{13}CO$ 1-0, $C^{18}O$ 1-0, CS 1-0, $N_2H^+$ 1-0) obtained with the Nobeyama Radio Observatory (NRO) 45-m telescope. To analyze these data, we apply statistical methods, the principal component analysis (PCA) and spectral correlation function (SCF), which are known to be useful to study underlying turbulent properties and to quantitatively characterize cloud structure. We will present the preliminary results of observations and analyses.