• ALGAE
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• v.21 no.3
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• pp.295-305
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• 2006

The purpose of the study is to understand the ecological characteristics of the early stage of community formation of epilithic diatoms. The samples were daily collected from a site of the Kumho River during 70 days from October 13 to December 21 in 2002. Total 126 diatom taxa collected in the study were classified into 114 species and 12 varieties belonging to 26 genera. About 80% of the taxa collected from the artificial substrata during the whole studying period were observed until tenth day from the start time of the study. In the study the successional process of the diatom community was divided into the three stages of the early, the middle and the later by cluster analysis based on the similarities in species composition among the communities. Cocconeis placentula Ehrenb. and C. placentula var. lineata (Ehrenb.) V.H., horizontally positioned species, would be divided into the early successional species, and Diatoma vulgre Bory, vertically positioned species, was the later successional species. Cell densities and species composition of the diatoms on the artificial substrata were closely similar to them of natural substrata after 40th day from the start time of the study.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.3
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• pp.36-45
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• 2018

Cemeteries in the United States are frequently observed near residential areas in cities even though they are spaces for the dead. However, it has not been fully studied when and how cemeteries were formed in the process of urbanization. Thus, this research aimed to investigate the spatial structure and historical change of cemeteries in Seattle due to urbanization, focusing on the contextual relationship between cemeteries and the surroundings according to two time periods: the early formation period of cemeteries in Seattle and the current period. For this, a mixed method was taken in this paper that combined historical analysis based on old references and historic maps with cluster analysis using GIS. In conclusion, the research found that early cemeteries of Seattle in downtown areas were introduced as by-products of urban formation, but the cemeteries in the suburbs were established ahead of urban growth and neighborhood development. In addition, Seattle's cemeteries not only showed a similar locational pattern to Seattle's shopping centers, but also were positively associated with population distribution given the Hot Spot Analysis. In other words, Seattle's cemeteries were originally located in rural areas, but later came to be located in urban areas, sustaining the landscape of American rural cemeteries.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.39 no.4
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• pp.10-18
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• 2002

Network topology design is a multiobjective problem with various design components. The components such as cost, message delay and reliability are important to gain the best performance. Recently, Genetic Algorithms(GAs) have been widely used as an optimization method for real-world problems such as combinatorial optimization, network topology design, and so on. This paper proposed a method of Multi-objective GA for Design of the network topology which is to minimize connection cost and message delay time. A common difficulty in multiobjective optimization is the existence of an objective conflict. We used the prufer number and cluster string for encoding, parato elimination method and niche-formation method for the fitness sharing method, and reformation elitism for the prevention of pre-convergence. From the simulation, the proposed method shows that the better candidates of network architecture can be found.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.73.2-73.2
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• 2012

Matusoka & Kawara (2010) showed that the number density of the most massive galaxies (log $M/M_{\odot}=11.5-12.0$) increases faster than that of the next massive group (log $M/M_{\odot}=11.0-11.5$) during 0 < z < 1. This appears to be in contradiction to another important empirical concept of "downsizing". We attempt to understand the two observational findings in the context of the hierarchical merger paradigm using semi-analytic techniques. Our models closely reproduce the result of Matusoka & Kawara (2010). Downsizing can also be understood as larger galaxies have on average smaller assembly ages but larger stellar ages. Our fiducial models further reveal the details on the history of stellar mass growth of massive galaxies. The most massive galaxies (log $M/M_{\odot}=11.5-12.0$ at z=0), which are mostly brightest cluster galaxies, obtain roughly 70% of their stellar components via merger accretion. The role of merger accretion monotonically declines with galaxy mass: 45% for log $M/M_{\odot}=11.0-11.5$ and 20% for log $M/M_{\odot}=10.5-11.0$ at z = 0. The specific accreted stellar mass rates via galaxy mergers decline very slowly during the whole redshift range, while the specific star formation rates sharply decrease with time. In the case of the most massive galaxies, merger accretion becomes the most important channel for the stellar mass growth at z ~ 2. On the other hand, in-situ star formation is always the dominant channel in the $L_*$ galaxies.

• Molecules and Cells
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• v.46 no.12
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• pp.746-756
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• 2023

A recent study revealed that the loss of Deup1 expression does not affect either centriole amplification or multicilia formation. Therefore, the deuterosome per se is not a platform for amplification of centrioles. In this study, we examine whether gain-of-function of Deup1 affects the development of multiciliated ependymal cells. Our time-lapse study reveals that deuterosomes with an average diameter of 300 nm have two different fates during ependymal differentiation. In the first instance, deuterosomes are scattered and gradually disappear as cells become multiciliated. In the second instance, deuterosomes self-organize into a larger aggregate, called a deuterosome cluster (DC). Unlike scattered deuterosomes, DCs possess centriole components primarily within their large structure. A characteristic of DC-containing cells is that they tend to become primary ciliated rather than multiciliated. Our in utero electroporation study shows that DCs in ependymal tissue are mostly observed at early postnatal stages, but are scarce at late postnatal stages, suggesting the presence of DC antagonists within the differentiating cells. Importantly, from our bead flow assay, ectopic expression of Deup1 significantly impairs cerebrospinal fluid flow. Furthermore, we show that expression of mouse Deup1 in Xenopus embryos has an inhibitory effect on differentiation of multiciliated cells in the epidermis. Taken together, we conclude that the DC formation of Deup1 in multiciliated cells inhibits production of multiple centrioles.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.866-873
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• 2008

2-Deoxystreptamine is a core aglycon that is vital to backbone formation in various aminoglycosides. This core structure can be modified to develop hybrid types of aminoglycoside antibiotics. We obtained three genes responsible for 2-deoxystreptamine production, neo7, neo6, and neo5, which encode 2-deoxy-scyllo-inosose synthase, L-glutamine: 2-deoxy-scyllo-inosose aminotransferase, and dehydrogenase, respectively, from the neomycin gene cluster. These genes were cloned into pIBR25, a Streptomyces expression vector, resulting in pNDOS. The recombinant pNDOS was transformed into a non-aminoglycoside-producing host, Streptomyces venezuelae YJ003, for heterologous expression. Based on comparisons of the retention time on LC-ESI/MS and ESI-MS data with those of the 2-deoxystreptamine standard, a compound produced by S. venezuelae YJ003/pNDOS was found to be 2-deoxystreptamine.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.71-71
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• 1995

The succinic semialdehyde dehydrogenase which is one of the key enzyme of GABA shunt in CNS has been purified from bovine brain homogeneously for the first time. The molecular mass of the native enzyme was estimated to be approximately 110,000 on gel filtration, The subunit molecular mass was determined by SDS-PAGE to be 54,000. These results indicate that the enzyme is a dimeric protein made up to identical subunits. Chemical modification studies of the enzyme suggest that the critical lysyl, connected with catalytic activity of the enzyme, The binding of IAF-SSDH(enzyme tagged with fluoreceine) to GABA transaminase which catalyzes the degradation of GABA was monitored by steady emission anisotropy. The changes of fluorescence anisotropy by interactions between two enzymes suggest that the formation of enzyme cluster must be invoved in the regulation of GABA concentration in brain tissues. The inhibitory effects of some antiepileptic and anticonvulsant drugs on the enzyme were also examined.

• Restorative Dentistry and Endodontics
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• v.32 no.1
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• pp.47-60
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• 2007

The purpose of this study is to compare and to evaluate the effect of pH and lactic acid concentration on the progression of artificial root caries lesion using polarizing microscope, and to evaluate the morphological changes of hydroxyapatite crystals of the demineralized area and to investigate the process of demineralization using scanning electron microscope. Artificial root caries lesion was created by dividing specimens into 3 pH groups (pH 4.3, 5.0, 5.5), and each pH group was divided into 3 lactic acid concentration groups (25 mM, 50 mM, 100 mM). Each group was immersed in acid buffer solution for 5 days and examined. The results were as follows : 1. Under polarized microscope, the depth of lesion was more effected by the lactic acid concentration rather than the pH. 2. Under scanning electron microscope, dissolution of hydroxyapatite crystals were increased as the lactic acid concentration increased and the pH decreased. 3. Demineralized hydroxyapatite crystals showed peripheral dissolution and decreased size and number within cluster of hydroxyapatite crystals and widening of intercluster and intercrystal spaces as the pH decreased and the lactic acid concentration increased. 4. Under scanning electron microscope evaluation of the surface zone, clusters of hydroxyapatite crystals were dissolved, and dissolution and reattachment of crystals on the surface of collagen fibrils were observed as the lactic acid concentration increased. 5. Under scanning electron microscope, demineralizatlon of dentin occurred not only independently but also with remineralization simultaneously. In conclusion, the study showed that pH and lactic acid concentration influenced the rate of progression of the lesion in artificial root caries. Demineralization process was progressed from the surface of the cluster of hydroxyapatite crystals and the morphology of hydroxyapatite crystals changed from round or elliptical shape into irregular shape as time elapsed.

• Proceedings of the Korean Vacuum Society Conference
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• 1994.02a
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• pp.43-43
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• 1994

We studied chemical reactio of Eu metal on the in situ cleaved CdTe(110) surface by pphotoemission sppectroscoppy using synchrotron radiation. The chamber was maintained with base ppressure $\leq$2${\times}$10-10 mb during the expperiment. The expperiment was carried out in pphoton Factory in Jappan. Core level pphotoemission sppectroscoppy was carried out with Al K${\alpha}$ Line. The CdTe simiconductor was determined to be pp-typpe with low dopping concentration from Hall measurement. We found that there are two reacted pphases of Te with Eu (related to divalent Eu and trivalent Eu, resppectively) from least square fitting of Te 4d sppectra, but three is no indication of Cd reaction. Trivalent Eu exists after roughly one monolayer depposition (600 sec. depposition time is considered as one monolayer), which is also observed at Eu 3d core level sppectra. Overlayer Eu is metallized after roughly 2 monolayers depposition, as can be deduced from the fact that metallic edge near Fermi level begins to appear. The intensity of core-level of Te decreases expponentially at the initial stage (near one monolayer) and after one monolayer depposition it decreases more slowly due to Te out-diffusion. We categorized the growth mode of Eu on CdTe as S-K growth mode (cluster formation after one monolayer deppisition) from the relative intensity pplot of Te 4d normalized to the cleaved surface. At cleaved surface band bending is already established due to surface defects. At first 100 sec. depposition time the shift toward lower binding side by 0.6 eV is found at all core level sppectra of all elements in semiconductor. This shift is considered as the re-adjustment of surface Fermi level to the pposition induced by Eu metal (0.2 eV above the valence band maximum).

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.208-216
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• 2019

This study investigated design factors and removal efficiencies of a sheet flow channel as natural type water improvement techniques in the stream. The channel was designed considering the design factors, then constructed and monitored in the test bed of the Osan stream's floodplain. Water lever, velocity, discharge, T-P, T-N, and NO₃-N were monitored and the removal efficiencies and design formula were suggested. Overall efficiencies of T-P, T-N, and NO₃-N range 10 - 20%, and they show consistent relations with residence times. Minimum velocity requirement is also suggested from the relation of algae conditions and velocities. Relation formula of residence time and removal efficiency will be applicable in the design and operation process of a sheet flow channel.