• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.2
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• pp.224-228
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• 2022

The feeding habits of the Pacific herring Clupea pallasii were examined based on 333 specimens collected from the coastal waters of the East Sea of Korea. Sampling was conducted in April, June, and September 2019 and February 2020. The total length of the C. pallasii ranged from 11.9 cm to 37.7 cm. Euphausiids (93.0%, index of relative importance, IRI), especially Euphausia, comprised the primary diet of the pacific herring, in addition to amphipods, copepods, and fishes. The dietary composition of C. pallasii showed non-significant differences by size. As body size of C. pallasii increased, the mean number of preys per stomach (mN/ST) and the mean weight of preys per stomach (mW/ST) tended to increase.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.53 no.1
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• pp.107-113
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• 2017

The feeding habits of larval (5.0~27.0 mm SL) Clupea pallasii were examined and 1,523 individuals were collected from November 2010 to March 2011 in the coastal water of Eastern Jinhae Bay, Korea. Larval C. pallasii were fed mainly on copepods that constituted 55.2% in IRI. Monogeneans were the second largest prey component, another prey items tintinnids, cladocerans and ostracoda. The results of analysis in ontogenetic changes exhibit high during the daytime, two small size classes (${\leq}10mm$, 10~15 mm) mainly fed copepods. while the percentage of coperpods decreased, monogeneans ratio increased in 15~20 mm size class. Feeding rate in diel difference of larval C. pallasii were high during the daytime.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.4
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• pp.498-506
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• 2015

We examined the feeding habits of larval Clupea pallasii using 148 specimens collected from December 2010 to April 2011 in the Nakdong River estuary, Korea. We found that larval C. pallasii [8.3-26.0 mm NL (notochord length)] are carnivorous, consuming mainly copepods, rotifers, cirripeds, and free-living flatworms (Macrostomida). Their diet also include small quantities of shrimp nauplii, tintinnids, cladocerans, and larval bivalves, among other food sources. To evaluate ontogenetic changes in dietary composition, we split larvae into four size groups: 8-12 mm, 12-16 mm, 16-20 mm, and > 20 mm NL. Rotifers were the most important prey items for the smallest group, but all other groups predominantly consumed copepods. The largest group frequently preyed on rotifers, cirripeds and flatworms, in addition to copepods. Larvae also showed bi-modal feeding, with peaks in the late afternoon and at midnight, wherein the mean number of prey per individual peaked at 1,800-1,900 h.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.5
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• pp.534-538
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• 2019

The distribution and characteristics of spawning beds of the Pacific herring Clupea pallasii, were observed by scuba diving in Jinhae Bay, Korea, with a focus on marine vegetation as adhesive substrates and egg density during the spawning season. In 2017, the fertilized eggs of C. pallasii were mainly found attached to the leaves of algae species Carpopeltis cornea, Gracilaria textorii, and Acrosrium polyneurum at the rocky shore in Wonpo-dong and Jeodo. The mean density of fertilized eggs in C. pallasii spawning beds was $153,776eggs/m^2$ off Wonpo-dong and $856,430eggs/m^2$ off Jeodo, giving an estimated total of $5.345{\times}10^9$ eggs in Wonpo-dong and $3.3074{\times}10^{10}$ eggs in Jeodo. We examined spawning beds with an area of $38,619m^2$ off Wonpo-dong and $34,760m^2$ off Jeodo. The choice of spawning bed may be affected by the composition of marine vegetation, such as the algal diversity of rocky shore environments, which could affect adhesive substrates and egg density during the spawning season. The results of the present study suggest that the spawning season of C. pallasii in Jinhae Bay is in January to mid-February.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.3
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• pp.370-374
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• 2015

Pacific herring Clupea pallasii eggs were attached to the gill net in Korean coastal waters off Busan. To conservation and management the eggs attached to the gill net, we followed the natural hatching in coastal waters from five days after fertilization to the hatching finished, where the temperature was $9^{\circ}C$. The diameter of fertilized eggs was 1.24-1.55 mm (n= 50), and had a segmented pale yellow yolk, no oil globule. Natural hatching had started by 10 days after fertilization. Mass hatching occurred around 11-12 days after fertilization, most of eggs separated from the gill net. Therefore, when the Pacific herring eggs attached in gill net, should be natural hatching-induced in coastal waters during 12-14 days.

• Development and Reproduction
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• v.18 no.4
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• pp.287-292
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• 2014

In this study, seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously calculated. Euclidean genetic distances within- and between-species were also calculated by complete linkage method with the sustenance of the hierarchical dendrogram program Systat version 13. The genomic DNA isolated from herring (Clupea pallasii), Korean anchovy (Coilia nasus) and large-eyed herring (Harengula zunashi), respectively, in the Yellow Sea, were amplified several times by PCR reaction. The hierarchical dendrogram shows three chief branches: cluster 1 (PALLASII 01, 02, 03, 04, 06 and 07), cluster 2 (NASUS 08, 09, 10, 11, 12, 13 and 14), and cluster 3 (ZUNASHI 15, 16, 17, 18, 19, 20, 21 and PALLASII 05). In three clupeid species, the shortest genetic distance displaying significant molecular difference was between individual PALLASII no. 03 and PALLASII no. 02 (0.018). Individual no. 06 of PALLASII was most distantly related to NASUS no. 11 (genetic distance = 0.318). Individuals from herring (C. pallasii) species (0.920) exhibited higher bandsharing values than did individuals from Korean anchovy (C. nasus) species (0.872) (P<0.05). As a result, this PCR analysis generated on the genetic data displayed that the herring (C. pallasii) species was widely separated from Korean anchovy (C. nasus) species. Reversely, individuals of Korean anchovy (C. nasus) species were a little closely related to those of large-eyed herring (H. zunashi) species.

• Korean Journal of Ecology and Environment
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• v.54 no.3
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• pp.209-220
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• 2021

Pacific herring, Clupea pallasii, a keystone species with significant ecological and commercial importance, is declining globally throughout much of its range. While traditional fishing equipment methods remain limited, new sensitive and rapid detection methods should be developed to monitor fisheries resources. To monitor the presence and quantity of C. pallasii from environmental DNA (eDNA) extracted from seawater samples, a pair of primers and a TaqMan^® probe specific to this fish based on mitochondrial cytochrome b (COB) sequences were designed for the real-time PCR (qPCR) assay. The combination of our molecular markers showed high specificity in the qPCR assay, which affirmed the success of presenting a positive signal only in the C. pallasii specimens. The markers also showed a high sensitivity for detecting C. pallasii genomic DNA in the range of 1 pg~100 ng rxn^-1 and its DNA plasmid containing COB amplicon in the range of 1~100,000copies rxn^-1, which produced linear standard calibration curves (r²=0.99). We performed a qPCR assay for environmental water samples obtained from 29 sampling stations in the southeastern coastal regions of South Korea using molecular markers. The assay successfully detected the C. pallasii eDNA from 14 stations (48.2%), with the highest mean concentration in Jinhae Bay with a value of 76.09±18.39 pg L^-1 (246.20±58.58 copies L^-1). Our preliminary application of molecular monitoring of C. pallasii will provide essential information for efficient ecological control and management of this valuable fisheries resource.

• Korean Journal of Ichthyology
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• v.32 no.1
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• pp.14-20
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• 2020

The purpose of this study is to clarify when spawning occurs of Clupea pallasii in the coastal waters of Jinhae Bay and Yeongun-ri, Tongyeong, Korea from December to February using SCUBA observation which is known as spawning season. Eighteen surveys of SCUBA diving observations have performed at 3 stations of Jinhae Bay from December 2017 to January 2018 and 24 times of survey at Yeongun-ri, Tongyeong from December 2017 to February 2018. In Jinhae Bay, average 740,274 eggs/㎡ fertilized eggs were observed in Judo Island and average 671,718 eggs/㎡ were observed in Gusan-myeon. On December 30, 2017, about 388,444 eggs/㎡ fertilized eggs were observed in Yeongun-ri, Tongyeong but no eggs were founded in next surveys. The results in this study suggest that spawning season of C. pallasii in the coastal waters of Jinhae Bay and Tongyeong begins in late December at average 7.7℃ water temperature.

• Korean Journal of Ichthyology
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• v.29 no.4
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• pp.267-271
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• 2017

Diel changes in feeding activity of juvenile Pacific herring, Clupea pallasii were investigated throughout the analysis of stomach contents of 301 fishes collected from the southeastern coast of Korea. Fish samples were collected every 3 hour over a 24 h period in summer using small bottom trawl. The ratio of empty stomach, stomach fullness, stomach contents index (SCI) and stomach fullness index (IF) of juvenile C. pallasii were varied markedly with day/night changes. The ratio of empty stomach tended be higher during nighttime, while the other values were higher at daytime, with showing peak before sunset. During daytime, the stomach contents mainly were made up of copepods and euphausiids, while the diets at midnight were composed of nearly digested and unidentifiable items.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.5
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• pp.614-620
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• 2015

The objective of this study was to investigate sensory and lipid characterizations of boiled-dried Pacific herring Clupea pallasii (PH) as a substitute for boiled-dried anchovies (A). The crude lipid content of commercial boiled-dried PH ranged from 1.3 to 5.1%, which was similar to that of commercial boiled-dried A (2.2-5.1%). The peroxide values (POVs) of commercial boiled-dried PH ranged from 20.5 to 129.1 meq/kg. The POVs of commercial boiled-dried PH suggest that boiled-dried PA of high quality should be controlled by a POV standard similar to that of boiled-dried A. The trichloroacetic acid soluble-nitrogen (TCA soluble-N) content of commercial boiled-dried PH ranged from 495.7 to 998.6 mg/100 g, which was high compared to that of commercial boiled-dried A (372.6-690.0 mg/100 g). Volatile basic nitrogen (VBN) content of commercial boiled-dried PH ranged from 8.4 to 28.1 mg/100 g, which was low compared to that of commercial boiled-dried A (16.8-33.0 mg/100 g). Results from the lipid, POV, TCA-soluble-N, and VBN content analyses suggest that boiled-dried PH could be used as a substitute for boiled-dried A.