• 약학회지
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• 제52권1호
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• pp.33-36
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• 2008

Staphylococcus aureus KH13 and Staphylococcus aureus KH28 were resistant to chloramphenicol, ampicillin, clindamycin, erythromycin, gentamicin, kanamycin, streptomycin, tobramycin, and norfloxacin. A plasmid (pKH13) and two plasmids (pKH14, pKH15) were isolated from Staphylococcus aureus KH13 and Staphylococcus aureus KH28, respectively and complete nucleotide sequences of three plasmids were determined. It was found that pKH13 and pKH15 mediated chloramphenicol resistance and pKH14 was a cryptic plasmid.

• 약학회지
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• 제30권6호
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• pp.317-322
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• 1986

To clarify resistance mechanisms of lincosamide antibiotics, it was examined whether lincosamide antibiotics was able to induce high resistance to macrolide and lincosamide antibiotics against EMR-1 strain of Bacillus species. And it was also examined whether the inducible resistance was plasmid-mediated or chromosome-mediated. This strain was identified as Bacillus licheniformis by its morphological and physiological characteristics. The subinhibitory concentrations of lincomycin and clindamycin induced high resistance in the strain to lincosamide antibiotics, but not to macrolide antibiotics. The inducible resistance was not eliminated by treating the strain with ethidium bromide, and plasmid was not identified by the alkaline lysis method of plasmid preparation. These results indicate, therefore, that the inducible resistance to macrolide and lincosamide antibiotics in the strain may be chromosome-mediated, not plasmid-mediated.

• 약학회지
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• 제37권6호
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• pp.621-624
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• 1993

The clirical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloroamphenicol, clindamycin. erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.14kb plasmid(pKH7) encoded resistance to chloramphenicol. The cleavage map of pKH7 was determined by restriction enzyme mapping techniques. The cleavage map is given for BstEll, Hindlll, Hpall, and Xbal. The above restriction endonucleases have a single site, but nucleases BamHl, Bgll, BglII, EcoRl, EcoRV, HaeIII, Hpal, Kpnl, Pstl, PvnII, Sall, Smal, and XhoI have no site on this plasmid.

• 약학회지
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• 제39권1호
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• pp.6-9
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• 1995

The clinical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.44 kb plasmid(pKH6) encoded resistance to tetracycline. The cleavage map of pKH6 was determined by restriction enzyme mapping techniques. The cleavage map is given for EcoRV, HindIII, HpaI, HpaII, KpnI and Xbal. Restriction endonucleases BamHl, BglI, BGIII, BstEII, EcoRI, HaellI, PstI, PvuII, SalI, Smal, and Xhol have no site on this plasmid. The restriction map revealed extensive structural homology between pKH6 and pT181.

• Pediatric Infection and Vaccine
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• 제16권1호
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• pp.87-91
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• 2009

세균성 근염은 화농성 관절염, 봉와직염, 골수염, 혈전 정맥염, 다발성 근염, 혈종, 좌상, 종양과 감별이 필요하며 진단에 MRI가 도움이 된다. 저자들은 A군 사슬알균에 의한 화농성 근염을 조기에 진단하여 clindamycin으로 치료하였고 심부정맥 혈전증까지 합병되었던 환자를 경험하였다. 성인에서는 증례로 보고된 바가 있지만 국내에서는 아직 보고가 없어 이를 보고하는 바이다.

• Pediatric Infection and Vaccine
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• 제16권1호
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• pp.1-5
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• 2009

Methicillin-resistant Staphylococcus aureus (MRSA), a leading cause of nosocomial infections, has been increasingly recognized in communities of the United States. This article will review the clinical spectrum and treatment of MRSA infections in children in the context of recent epidemiological changes of MRSA infections. In general, community-associated (CA) MRSA most frequently causes skin and soft tissue infections and has an increased association with invasive infections, particularly pneumonia and musculoskeletal infections. Hospital-associated (HA) MRSA strains tend to be associated with bloodstream infections, pneumonia, and surgical site infections. Different from the United States, CA-MRSA infections are not common in Korea (only 5.9%); however, there are some CA-MRSA clones that are different from HA-MRSA clones in Korea and from CA-MRSA clones in other countries. The treatment of MRSA infections should be guided by antimicrobial susceptibility testing, the site of infection, and the infection severity. Vancomycin is the treatment of choice for invasive MRSA infections. Other agents such as trimethoprim-sulfamethoxazole, clindamycin, linezolid, quinupristin-dalfopristin, and daptomycin have been used for some conditions.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.542-544
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• 2023

Methicillin-resistant Staphylococcus pseudintermedius Z0118SP0130 was isolated from eye specimen of a companion dog in South Korea. The complete genome of Z0118SP0130 consisted of a 2,663,277 bp chromosome and there was no plasmid. The strain was identified as the sequence type 45 and contained a mecA gene which comprised of staphylococcal cassette chromosome mec type Vb (5C2&5). Antimicrobial resistance to erythromycin, clindamycin, quinupristin-dalfopristin, trimethoprim-sulfamethoxazole, mupirocin, oxacillin, streptomycin, and gentamicin was observed in the strain.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.545-547
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• 2023

Staphylococcus epidermidis is a normal flora of human skin and is occasionally associated with pathogenic infections. We report the complete genome sequence of methicillin-resistant Staphylococcus epidermidis strain Z0118SE0272 isolated from the residential environment sharing by a companion dog and dwellers. Resistance to cefoxitin was observed in the strain, whereas it was susceptible to erythromycin, clindamycin, quinupristin-dalfopristin, trimethoprim-sulfamethoxazole, mupirocin, vancomycin, teicoplanin, linezolid, and tigecycline. The strain Z0118SE0272 identified as sequence type 130 possessed the mecA gene responsible for methicillin resistance, which composed the new type of staphylococcal cassette chromosome mec elements lacking mecRI.

• 대한의생명과학회지
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• 제30권1호
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• pp.32-35
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• 2024

In vitro antimicrobial activities of hot water extracts of Chamaecyparis obtuse, for methicillin-resistant Staphylococcus aureus (MRSA) was compared to commonly used conventional antimicrobial agents. All MRSA was susceptible to linezolid or vancomycin, but also to erythromycin. MIC range and MIC₉₀ to erythromycin, clindamycin, levofloxacin, tetracycline for MRSA were each 4 ㎍/mL, 2 ~ >128 ㎍/mL, ≤0.06 ~ >128 ㎍/mL, 0.25 ~ >128 ㎍/mL, 0.25~64 ㎍/mL and 4 ㎍/mL, .128 ㎍/mL, >128 ㎍/mL, >128 ㎍/mL, 64 ㎍/mL. The hot water extracts of leaf of C. obtuse had the lowest MIC range, MIC₅₀, and MIC₉₀ (0.125 µL/mL) for the MRSA tested, and it was possible more potent than various conventional antimicrobial agents. Screen antibacterial drug candidate with high antibacterial activity such as derivatives of C. obtuse leaf extract such as terpinen-4-ol or using combined therapy with commercialized antibacterial agents will likely be helpful in treating refractory MRSA infections.

• 대한임상검사과학회지
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• 제48권2호
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• pp.82-87
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• 2016

Enterotoxigenic Bacteroides fragilis (ETBF)는 병독소 인자로 알려진 장독소를 생성하는 균종이다. B. fragilis enterotoxin (BFT)은 bft-1, bft-2 및 bft-3 세 개의 유전자 아형들이 밝혀졌다. 본 연구에서는 임상 검체에서 분리된 B. fragilis에서 bft 유전자의 유무와 BFT 음성 및 양성 균주의 항균제 내성을 조사하였다. 국내의 한 대학병원에서 8년간(2006~2013년) 장외 검체에서 분리된 B. fragilis는 총 537주이었다. 다중중합연쇄반응으로 시험하여 bft 유전자 아형을 확인하였다. BFT 음성 74주와 양성 33주를 포함한 B. fragilis 107주의 항균제 감수성은 CLSI 한천희석법으로 시험하였다. 임상 검체에서 분리된 B. fragilis의 bft 유전자 검출율은 30% 이었고, 이 중 혈액과 혈액 외 장외 검체 분리주에서는 각각 33%, 29% 이었다. ETBF 중에서 가장 흔한 아형은 bft-1 이었고, 그 다음은 bft-2, bft-3 순이었다(bft-1: 77%, bft-2: 14%, bft-3: 9%). BFT-음성과 양성 균주의 내성률은 일부 항균제에 대해서 차이가 있었다(BFT-음성 균주: piperacillin-tazobactam 3%, cefoxitin 5%, imipenem 1%, clindamycin 38%; BFT-양성 균주: piperacillin-tazobactam 3%, cefoxitin 6%, imipenem 3%, clindamycin 42%). BFT-음성 및 BFT-양성 균주 모두는 chloramphenicol과 metronidazole에 대한 내성은 없었다. 결론적으로, 혈액 분리주에서의 ETBF 검출율은 혈액 외 장외 검체 분리주에서와 비슷하였고, 가장 흔한 유전자 아형은 bft-1 이었다. 항균제 내성은 BFT 양성 균주가 음성 균주보다 대체로 높았으나 통계학적으로 유의한 차이는 없었다.