• Title/Summary/Keyword: Clematis mandshurica

Search Result 15, Processing Time 0.022 seconds

Anti-inflammatory Effect and Inhibition of Melanin Biosynthesis of Clematis mandshurica (위령선 추출물의 항염활성 및 멜라닌 생성 억제효과)

  • Kim, Ye Rim;Hong, Yun Jung;Yang, Ki Sook
    • YAKHAK HOEJI
    • /
    • v.58 no.1
    • /
    • pp.47-52
    • /
    • 2014
  • Clematis mandshurica (Ranunculaceae) has traditionally been used as a remedy for antidiuretic, antifungal, rheumatic conditions and alleviate pain. We carried out to evaluate the anti-oxidative effect, anti-inflammatory effect and anti-melanogenic effect of ethanol extract and solvent fractions of Clematis mandshurica. The ethanol extract and the dichloromethane fraction of Clematis mandshurica showed an anti-oxidative effect in DPPH assay, the inhibitory activity of nitric oxide (NO) production in lipopolysaccharide (LPS) activated RAW 264.7 cell, and melanin synthesis and tyrosinase activity of B16F10 melanoma cells. They reduced NO production and melanin content in a dose-dependent manner at concentrations of $2.5{\sim}10{\mu}g/ml$. They also suppressed iNOS and tyrosinase protein and m-RNA expressions dose dependently, assayed by western blot analysis and RT-PCR experiment.

Development of PCR based Genetic Marker for Discrimination of Manchurian Clematis, Clematis terniflora var. mandshurica and Three-leaf clematis, Clematis apiifolia (으아리와 사위질빵 판별을 위한 PCR 기반의 마커 개발)

  • Dae-Ju Oh;Eun Bi Jang;Jong-Du Lee;Hyejin Hyeon;Yong-Hwan Jung
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2021.04a
    • /
    • pp.16-16
    • /
    • 2021
  • To distinguish manchurian clematis, Clematis terniflora var. mandshurica and three-leaf clematis, C. apiifolia, we collected 9 nuclear ITS sequences and two sequences of trnQ-trnH intergenic spacer and trnH region in GenBank database. Those sequences were aligned to find differences between those of C. terniflora var. mandshurica and C. apiifolia. Two primer pairs were newly designed base on the differences between two species and conducted multiplex PCR. The size of amplified fragments using generated primers were 380 base pairs (only three-leaf clematis) and 189 base pairs (both species). This genetic marker based on PCR is useful to discrimination of C. terniflora var. mandshurica and C. apiifolia

  • PDF

The Bibiographical Investigation of effect of Clematis mandshurica Maxim (위령선(威靈仙)의 약리(藥理)에 대한 사상의학적(四象醫學的) 고찰(考察))

  • Jung, Kuk-yung;Song, Il-byung
    • Journal of Sasang Constitutional Medicine
    • /
    • v.10 no.2
    • /
    • pp.151-162
    • /
    • 1998
  • Purpose and Method : We have many difficulty of using the existing medical Hurbs based on the theory of Yin-yang and the five elements, this is why we still do not explain the Sasang Constitutional medical Hurb Classification and do not have the Sasang Constitutional Pharmacology exactly, so we easily enter into a dispute and confusion. So through literary consideration about clematis mandshurica Maxim. I try to objectify Sasang Constitutional Clasification of Clematis mandshurica Maxim and the spirit of using Clematis mandshurica Maxim and common property of Sasang Constitutional Medical Hurb and try to find out a clue that search the effect of other Sasang Constitutional Medical Hurb. Result : Qi(氣) and mi(味) of Clematis mandshurica Maxim has bitter and hot taste and have won Qi(溫氣), the color is dark, the using portion of clematis mandshurica Maxim is root as medial Hurb. So Clematis mandshurica Maxim fall down from lung and divied impurity and purity and able to remove the symptom that dryness and fever is solidified like Magnoliae cortex(厚朴). Clematis mandshurica Maxim have the effect of awakening Jin-Qi(眞氣) of lung and divide impurity and purity of Qi(氣) and ack(液) and improve the fuction and structure of Taeumin(太陰人) I think that the method of literay consideration on objectification of Sasang Constitution Pharmacology is of great value.

  • PDF

Mechanism of Herbal Acupuncture of Clematis Mandshurica Maxim. Water Extract by Stimulation of Sinsu (BL 23) Loci Subcutaneously as Dual Inhibitor of Proinflammatory Cytokines on Adjuvant Arthritis in Rats

  • Cho, Soo-Won;Kim, Kap-Sung
    • The Journal of Korean Medicine
    • /
    • v.27 no.4
    • /
    • pp.182-190
    • /
    • 2006
  • Objective : Based on immunological mechanisms, this study examined whether subcutaneous (s.c.) injection of Clematis mandshurica Maxim. water extract (CMA) has anti-inflammatory effects, and its effect on $TNF-{\alpha}$, IL-1 and IL-10 release from synoviocytes on adjuvant arthritis (AA) in the rat. Methods : Complete Freund's adjuvant was used to induce AA in rats. Synoviocytes were separated by the method of collagenase and DNase digestion Synoviocytes proliferation was assayed by 3-(4, 5 dimethylthiazol 2 yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. $TNF-{\alpha}$, IL-1 and interleukin-10 (IL-10) production of synoviocytes was measured with ELISA. The expression of IL-10 mRNA of synoviocytes was determined using RT PCR. Results : There were significant secondary inflammatory reactions in AA rats, accompanied by the decrease of body and immune organs weight simultaneously. Synoviocytes proliferation of AA rats significantly increased, and the levels of $TNF-{\alpha}$ and IL-1 in supernatants of synoviocytes in AA rats were also elevated compared with the sham group. The administration of CMA (2, 5, 10 mg/kg, s.c.) reduced the above changes significantly. In contrast to $TNF-{\alpha}$ and IL-1, IL-10 production and the level of its mRNA of synoviocytes in AA rats apparently decreased. CMA (2, 5, 10 mg/kg, s.c.) markedly increased IL-10 in synoviocytes at protein and transcription level. Conclusion : The results indicate that CMA has a beneficial effect on rat AA due to modulating inflammatory cytokine production of synoviocytes, which play a crucial role in the pathogenesis of this disease.

  • PDF

Effects of Herbal Acupuncture of Clematis Mandshurica Maxim. at Sinsu-hyul (BL 23) on Adjuvant Arthritis in Rats.

  • Kim, Kap-Sung;Lee, Seung-Deok;Lee, Chang-Hwan
    • The Journal of Korean Medicine
    • /
    • v.27 no.4
    • /
    • pp.174-181
    • /
    • 2006
  • Objective : Based on the immunological dysfunction in RA and the immunological feature of Clematis mandshurica Maxim. herbal acupuncture solution (CM-HAS), this study examined whether subcutaneous (s.c.) administration of CM-HAS has anti-inflammatory effects on adjuvant arthritis (AA) in the rat. Methods : Complete Freund's adjuvant was used to induce AA in rats. Secondary paw swelling of AA rats was measured, and polyarthritis index was scored. The administration of CM-HAS (2, 5, 10 mg/kg) to the Sinsu-hyul (BL 23) acupuncture point subcutaneously (s.c.) inhibited the inflammatory response and restored the weight of body and immune organs of AA rats. In this study. inhibitory effect of CM-HAS on cyclooxygenase-2 (COX-2) was evaluated. The plant material selected for this study has been used in Korean medicine for the treatment of various diseases that are considered as inflammatory in nature, e.g. asthma, arthritis, rheumatism, fever, edema, infections, snakebite and related inflammatory diseases. Results : There were significant secondary inflammatory reactions in AA rats, accompanied with the simultaneous decrease of body and immune organs weight. The administration of CM-HAS (2, 5, 10 mg/kg, s.c.) improved the above changes significantly. CM-HAS showed inhibitory activity against COX-2, which supports their traditional uses. In this study, aspirin and indomethacin were used to contrast with CM-HAS as the COX-2 inhibitors. Conclusion: The findings of this study may explain at least in part why CM-HAS has been traditionally used for the treatment of inflammatory conditions in traditional Korean and Chinese medicine.

  • PDF

Effects of Clematis mandshurica Rupr. on Nitric Oxide Synthase in the Periaqueductal Gray of Collagenase-induced Rat Osteoarthritis Model (위령선 약침이 콜라겐으로 유도된 골관절염 모델에서 흰쥐의 PAG 영역에서 NOS 발현에 미치는 영향)

  • Yang, Kook-Jung;Kim, Soon-Joong;Seo, Il-Bok;Park, Se-Keun;Kim, Jeong-Seon;Seo, Jung-Chul;Choi, Sun-Mi;Lee, Hye-Jung;Kim, Ee-Hwa
    • Korean Journal of Acupuncture
    • /
    • v.22 no.4
    • /
    • pp.109-116
    • /
    • 2005
  • 목적 : 골관절염은 진통을 수반하는 퇴행성 관절질환이며, 장애를 일으키는 주요한 원인이 된다. 또한 노인들에 있어서 골관절염은 매우 흔한 질환이라 할 수 있다. Nitric oxide(NO)는 Nitric Oxide Synthase(NOS)에 의하여 칼슘의존성통로를 통하여 L-arginine 으로부터 합성되어지며, NO는 중추신경계에 있어서 중요한 세포사이의 전달자이다. 방법 :본 연구에서는 위령선 으로부터 추출한 액이 콜라겐으로 유도된 관절염에 걸린 쥐의 dorsolateral periaqueductal gray(DL-PAG) 영역에서 nNOS(neuronal NOS)와 NOS에 대하여 미치는 영향 을 nNOS immunohistochemistry와 nicotinamide adenine dinucleotide phosphate-diaphorase(NADPH-d) 검사법을 통하여 조사하였다. 결과 : 골관절염이 유발된 흰쥐의 DL-PAG 영역에서 nNOS와 NOS의 발현억제가 관찰되었으며, 위령선이 콜라겐으로 유도된 골관절염에서 감소된 nNOS와 NOS의 발현이 증가되었다. 결론 : 본 연구를 통하여 위령선은 골관절염이 유발된 흰쥐의 DL-PAG에서의 nNOS와 NOS의 발현에 영향을 미친다는 결과를 얻을 수 있었다.

  • PDF

The Experiment of Vine for Covering the Traffic Noise Barrier (방음벽 녹화를 위한 덩굴식물 활용성 연구)

  • Jung, Tae-Geun;So, Jae-Hyun;Lee, Eun-Jung;Jeon, Gi-Seong
    • Journal of the Korean Society of Environmental Restoration Technology
    • /
    • v.2 no.1
    • /
    • pp.72-82
    • /
    • 1999
  • The objective of this study was to covering of vine the traffic noise barrier and analyse of their growth characteristics. For the purpose of the experiments, chosen were 28 plants. In January 1996 to December 1998, the nursery seedbeds on the Chonju Arboretum in Korea Highway Corporation were seeding and cutting with those species, and a few species were carried out Honam Highway field experiments. The results are summarized as follows ; The germination ratio of seedbed experiments were Wisteria floribunda(88%), Lonicera japonica(86%), Parthenocissus tricuspidata, Paederia scandens(85%), Celastrus orbiculatus(76%), Clematis terniflora var. denticulata(52%), Clematis mandshurica(44%) respectively. The rooting ratio of cutting experiments were Trachelospermum asiaticum var. intermedium(88%), Parthenocissus quinquefolia(87%), Trachelospermum asiaticum var. asiatica(85%), Kadsura japonica(82%), Hedera rhombea(81%), Euonymus fortunei var. radicans(83%), Trachelospermum jasminoides var. pubescens(80%), Lonicera japonica cv. Aureo-reticulata(80%), Trachelospermum asiaticum var. majus(78%), Euonymus radicans cv. Aureo-marginata(66%), in descending order. As a conclusion, this study shows that the traffic noise barrier was effected by Paederia scandens, Lonicera japonica, Parthenocissus quinquefolia, Wisteria floribunda, Parthenocissus tricuspidata in the field experiments and the others were necessary to maintainable management.

  • PDF

Preparation of Antiinflammatory Herbal Drug, SKI306X. (항염작용을 갖는 신규 생약복합제 SK1306X의 분리 및 항염작용)

  • 박광식;김환수;안재석;김택수;박병욱;곽의종;한창균;조용백;김기협
    • YAKHAK HOEJI
    • /
    • v.39 no.4
    • /
    • pp.385-394
    • /
    • 1995
  • Antiinflammatory activities of the solvent fractionates of several herbal medicines were investigated and SKI306X was prepared from the active principles of three herbal medicines, Prunella vulgaris, Trichosantlies kirilowii and Clematis mandshurica. SK1306X was shown to have strong inhibitory effects on acetic acid-induced pain, carrageenan-induced paw edema and adjuvant induced arthritis. LD50 of SKI306X was more than 5 g/kg in rat, so generally nontoxic. Chemical analysis revealed that oleanolic acid and rutin, which are known to have various antiinflammatory activities, were contained in it. These results suggest SK1306-X may become a useful drug for the treatment of inflammatory diseases such as rheumatoid arthritis.

  • PDF

Acute Toxicity of SKI306X, an Antiinflammatory Herbal Extract, in Rats (랫드에서 생약복합제 SKI306X의 급성독성에 관한 연구)

  • 안재석;김훈택;조용백;김환수;박광식;박병욱
    • Biomolecules & Therapeutics
    • /
    • v.4 no.1
    • /
    • pp.32-35
    • /
    • 1996
  • SKI306X is a herbal extract prepared from three herbs Clematis mandshurica, Trichosanthes kirilowii and Prunella vulgaris. It showed strong antiinflammatory actions on carrageenan-induced edema, acetic acid-induced pain, adjuvant-induced arthritis, and oxygen radical-generated reactions. In this study, the acute toxicity of SKI306X was evaluated in rats by a single oral administration. Thirty male and thirty female rats were divided into 6 groups according to the dose levels, respectively. After oral administration of SKI306X with several doses (5.0 g/kg, 3.3 g/kg, 2.2 g/kg, 1.5 g/kg, 1.0 g/kg), mortality, clinical signs, body weight, and gross findings in organs were examined. No toxic effect was shown in terms of mortality, clinical signs, body weight changes and gross findings. It is suggested the LD$_{50}$ of SKI306X would be more than 5.0 g/kg in rats.s.

  • PDF