The majority of freshwater ornamental fish are imported and distributed domestically, causing high risk of exposure to exotic pathogens and drug resistant bacteria in Korea. Aeromonas hydrophila is known as a common species of fresh water bacteria and opportunistic fish pathogen, as well as a species causing zoonotic infection. In this study, we isolated motile aeromonads from various imported freshwater ornamental fish and studied the characters of the isolates. Imported freshwater ornamental fish were purchased on day 1 after the fish were deposited in the aquarium. Bacteria were isolated from the liver, kidney and spleen of fish using 0.5% NaCl containing tryptic soy agar medium. Bacteria were grouped on the basis of their morphological characteristics. The colonies with clear zone on starch-ampicillin agar (SA agar) were tentatively identified as Aeromonas spp. Two hundred and twenty-six strains, about 70% of total isolates were assumed to be Aeromonas spp. Nine isolates were further identified based on the result of the API 20E test and PCR using primers specific for A. hydrophila 16S rRNA gene. The isolates were identified as A. hydrophila and the API 20E test showed differences in trisodium citrate, D-sucrose, D-melibiose, amygdalin and L-arabinose availability between the nine isolates and standard A. hydrophila. The susceptibilities of the isolated bacteria to 10 antibacterial agents were confirmed by the disk diffusion method. Isolated strains were found to be resistant to amoxicillin and ampicillin and sensitive to florfenicol. However, 7 isolates showed multiple drug resistances to erythromycin, oxytetracycline, nalidixic acid etc. Pathogenicity of the isolates was determined by the artificial challenge test on goldfish (Carassius auratus). Three isolates caused 60 ~ 80% mortality in goldfish within 5 days after the initiation of challenge. These results indicate that multiple drug resistant, highly pathogenic and exotic A. hydrophila can spread to domestic aquarium and the preventive treatment of fish before sale is necessary.
According to the content of sugar and starch of each positional leaf sheath and internode at heading and 4 weeks after it using IR667-Suwon 214 (high yielding var. having tropical Indica parantage) and Jinheung (local leading var. temperate Japonica) rice grown in various cultivation seasons the suitability of grouping into the high sugar type (sugar>starch), sugar, tendency (increasing tendency in sugar content), high starh type (starch>sugar) and starch tendency (increasing tendency in starch) in carbohydrate metabolism was reexamined as follows. 1. Sugar tendency appeared strongly in IR667 than Jinheung, internode than leaf sheath, late season cultivation than early season, 4 weeks after than heading and high temperature than low temperature. Thus at heading, leaf sheath and internode of Jinheung in early and late season cultivation were high starch type, and lower internode in early season cultiattion and leaf sheath and internode in late season for IR667 were high sugar type. In very late season all internodes of both varieties except 1st internode of Jinheung at heading were high starch type. At four week after heading all leaf sheaths except 1st and 4th one of Jinheung and all internodes were high sugar type. High sugar type was intensified 4 weeks after heading in leaf sheaths than in internodes of IR667 in early season and of both varieties in late season. 2. The upper three leaf sheaths and internodes seem to work in the same way for carbohydrate translocation. Among them upper ones showed sugar tendency at heading and starch tendency 4 weeks after heading and it was clear in Jinheung. 3. The later the cultivation season, the higher the carbohydrate content (sugar+starch), and such tendency was clear 4 weeks after heading and in IR667, suggesting teanslocation inhibition by low temperature. 4. Grain filling rate (weight increase per day) was more rapid in early season cultivation and IR667 took shorter days to reach maximum rate. 5. The later the cultivation season, the greater the percent contribution of carbohydrate before heading to yield and it was always greater in IR667, a leaf sheath type. 6. Sugar and starch ratio appears to be determined principally by metabolic characteristics of variety according to growth process and secondly but considerably by environmental factors.
Korean Journal of Agricultural and Forest Meteorology
/
v.18
no.4
/
pp.233-241
/
2016
Experiments were carried out to quantify the topographic effects on attenuation of sunshine in complex terrain and the results are expected to help convert the coarse resolution sunshine duration information provided by the Korea Meteorological Administration (KMA) into a detailed map reflecting the terrain characteristics of mountainous watershed. Hourly shaded relief images for one year, each pixel consisting of 0 to 255 brightness value, were constructed by applying techniques of shadow modeling and skyline analysis to the 3m resolution digital elevation model for an experimental watershed on the southern slope of Mt. Jiri in Korea. By using a bimetal sunshine recorder, sunshine duration was measured at three points with different terrain conditions in the watershed from May 15, 2015 to May 14, 2016. The brightness values of the 3 corresponding pixel points on the shaded relief map were extracted and regressed to the measured sunshine duration, resulting in a brightness-sunshine duration response curve for a clear day. We devised a method to calibrate this curve equation according to sky condition categorized by cloud amount and used it to derive an empirical model for estimating sunshine duration over a complex terrain. When the performance of this model was compared with a conventional scheme for estimating sunshine duration over a horizontal plane, the estimation bias was improved remarkably and the root mean square error for daily sunshine hour was 1.7hr, which is a reduction by 37% from the conventional method. In order to apply this model to a given area, the clear-sky sunshine duration of each pixel should be produced on hourly intervals first, by driving the curve equation with the hourly shaded relief image of the area. Next, the cloud effect is corrected by 3-hourly 'sky condition' of the KMA digital forecast products. Finally, daily sunshine hour can be obtained by accumulating the hourly sunshine duration. A detailed sunshine duration distribution of 3m horizontal resolution was obtained by applying this procedure to the experimental watershed.
Observation of hydrometeors' behavior in the atmosphere is important to understand weather and climate. By conventional observations, we can get the distribution of water vapor at limited number of points on the earth. In this study, the precipitable water has been estimated from the split window channel data on GMS-5 based upon the technique developed by Chesters et al.(1983). To retrieve the precipitable water, water vapor absorption parameter depending on filter function of sensor has been derived using the regression analysis between the split window channel data and the radiosonde data observed at Osan, Pohang, Kwangiu and Cheju staions for 4 months. The air temperature of 700 hPa from the Global Spectral Model of Korea Meteorological Administration (GSM/KMA) has been used as mean air temperature for single layer radiation model. The retrieved precipitable water for the period from August 1996 through December 1996 are compared to radiosonde data. It is shown that the root mean square differences between radiosonde observations and the GMS-5 retrievals range from 0.65 g/$cm^2$ to 1.09 g/$cm^2$ with correlation coefficient of 0.46 on hourly basis. The monthly distribution of precipitable water from GMS-5 shows almost good representation in large scale. Precipitable water is produced 4 times a day at Korea Meteorological Administration in the form of grid point data with 0.5 degree lat./lon. resolution. The data can be used in the objective analysis for numerical weather prediction and to increase the accuracy of humidity analysis especially under clear sky condition. And also, the data is a useful complement to existing data set for climatological research. But it is necessary to get higher correlation between radiosonde observations and the GMS-5 retrievals for operational applications.
As the physical and mechanical properties of lightweight air-mixed soil change in the procedure of transportation of mix truck, it is necessary to assure whether the properties during construction satisfy those in design. In this study, variations of properties of mixed soil after transportation by mix truck are proved by field test. Lightweight air-mixed soil used field test the unit weight of $9.0{\pm}1.0kN/m^3$, the flow value of $190{\pm}20mm$ was produced. To analyze variations of properties of mixed soil the unit weight and flow value of the sample before and after transport was measured unconfined compressive strength tests were performed. Mixing time was 19~175 minutes diversified. As the test results, it is known that the density, the flow value and the unconfined compressive strength of lightweight air-mixed soil change by transportation, but these values satisfy the specifications of material of air-mixed soil. After transportation the average value of the unit weight and flow value change in the flow of the $(+)0.10kN/m^3$, 4.8 mm respectively, the average change in the unit weight and the flow value due to the mixing time was constant. And unconfined compressive strength of 28-day specimen increases from 20 to $150kN/m^2$. But, these values do not have some clear relationship with the transportation time within 175 minutes which is longest test time. Consequently, Within 175 minutes the changes of properties by transportation are too small to show some problems in the construction field.
KSCE Journal of Civil and Environmental Engineering Research
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v.33
no.6
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pp.2455-2464
/
2013
This study is focused on the effect of reformation of public transportation system on the efficiency of bus route system as a result of change of government subsidy scheme. Two types of government subsidy scheme has been considered and before and after analysis by Data Envelopment Analysis technique has been carried out for bus routes in Ulsan Metropolitan City. The analysis output has shown that there is no clear evidence to clarify the relationship between the change of government subsidy scheme and the efficiency of bus route system. There are two types of backgrounds may be suggested for this situation such as more longer period necessary to identify the policy effect and limitation to increase the number of passenger in a provincial city. Tobit regression analysis has also been conducted to discover the most effective variables for maximum efficiency and three variables including route length, frequency per vehicle per day, and headway are found to be influential. It is also seen that strategy to minimising input factors and service upgrade plan such as shortened route length and headway, increasing frequency per vehicle are necessary to develop the efficiency of bus routes operated in Ulsan city.
Kim, Gum Hi;Yoon, Seok-Jun;Ahn, Hyeong-Sik;Lee, Jun-Young;Park, Hyeung-Keun;Suh, Kyung-Suk
Quality Improvement in Health Care
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v.11
no.1
/
pp.32-45
/
2004
Objective : The aim of this study were to measure quality of life(QOL) in liver transplant recipients, to compare QOL between living donor liver transplant recipients and cadaveric liver transplant recipients and to investigate whether SF-36 may be used as a disease-specific instrument in liver transplant recipients. Methods : We conducted a single-center cross-sectional study of 133 LT recipients ages 13 to 65 years, all of whom had had Liver Transplantation(LT) at least 1 months previously. QOL was assessed using a self-completion questionnaire consisting of the Bang Whal Ran(1991) instruments and the 36-Item Short-Form Health Survey(SF-36) health status profile measure. We investigated whether the SF-36 instrument may be used as a disease-specific instrument in LT recipients. Individual scale scores range from 0 to 100, with higher score reflecting better health. Data on demographics, clinical status at pre transplantation 1 day, post transplantation clinical status, and graft function were collected to identify predictors of post transplantation QOL. Results : Standard measures for test-retest reliability, internal consistency, and discriminant and concurrent validity were examined. The reliability of the SF-36, as measured by test-retest correlation(Pearson coefficients: 0.729, p=0.002) and by internal consistency(Cronbach's alpha: 0.9431) exceeded conventional acceptability criteria. The correlation between domain scores of SF-36 and the Bang Whal Ran(l991) was clear and logical in that the clinical characteristics of SF-36 strongly correlated with the clinical component summary score of the Bang Whal Ran(l991)(r = 0.8155, P<.01). SF-36 scale scores were compared between Cadaveric Liver Transplant recipients and Living Donor Liver Transplant recipients. Donor types of post LT did not influence HRQOL(p>0.05). 87% of the liver transplant recipients were satisfied to get LT. Satisfaction of post LT showed significantly greater HRQOL(p<0.001). Conclusion : SF-36 is found reliable and valid. This study indicates thet Donor Type did not influence HRQOL after LT. The information gained from this study will help us to better define expectations and the clinical course after liver transplantation to patients and their families.
Journal of The Korean Association of Information Education
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v.11
no.1
/
pp.21-28
/
2007
Along with the development of telecommunication technology, educational role has been considerably changed and NEIS is introduced and operated for the digital educational administration. However, the function of NEIS is so limited when operated in each school. As a result, Korean government prepares the plan to install and operate a new School Affairs System by separating 3 areas from 27 areas of NEIS. School Affairs System has been used in schools since March, 2006. In this regard, this study attempts to analyze the current status and tasks of using the system in elementary schools. The result of the study shows that about 67% teachers use the system once or twice a day. Most of teachers answer that they get help from other teachers if they have any problem in operating the system. Many teachers affirmatively answer to the design and function of the system, and they are satisfied with the convenience and efficiency of the system. However, they feel difficulty to be familiar with the system sue to the frequent change. The importance of electronic certificate and personal information is well recognized and managed in terms of the security of the system. Facilities need to be well equipped, and information exchange system need to be developed to accept each school's request to improve the function of the system. In addition, applicable laws need to be arranged, clear guideline of educational administration agency and principal's strong intention are required to relieve teachers' work load.
Objective : Neuropathic pain can be caused by a partial peripheral nerve injury. This kind of pain is usually accompanied by spontaneous burning pain, allodynia and hyperalgesia. It is not clear that scolopendrid aqua-acupuncture can control neuropathic pain effectively. The purpose of this study is to examine if scolopendrid aqua-acupuncture may be effective to the neuropathic pain (mechanical allodynia, cold allodynia) in a rat model of neuropathic pain. Methods : To produce the model of neuropathic pain, under isoflurane 2.5% anesthesia, tibial nerve and sural nerve was resected. After the neuropathic surgery, the author examined if the animals exhibited the behavioral signs of allodynia. The allodynia was assessed by stimulating the medial malleolus with von Frey filament and acetone. Three weeks after the neuropathic surgery, scolopendrid aqua-acupuncture was injected at Hwando(GB30) one time a day for one week. After that the author examined the withdrawl response of neuropathic rats' legs by von Frey filament and acetone stimulation. And also the author examined c-fos in the midbrain central gray of neuropathic rats and the change of WBC count in the blood of neuropathic rats. Results & Conclusion : 1. The scolopendrid aqua-acupuncture injected at Hwando(GB30) decreased the withdrawl response of mechanical allodynia in SHA-1, SHA-2 and SAH-3 group as compared with control group. 2. The scolopendrid aqua-acupuncture injected at Hwando(GB30) decreased the withdrawl response of chemical allodynia(cold allodynia) in SHA-1, SHA-2 and SAH-3 group as compared with control group. 3. The scolopendrid aqua-acupuncture injected at Hwando(GB30) showed the significant difference between sham group and control group(p=0.01), sham and SHA-3 group(p=0.026), control group and SHA-1 group(p=0.01), control group and SHA-2 group(p=0.024) in the c-fos expression. 4. The scolopendrid aqua-acupuncture injected at Hwando(GB30) showed the significant difference between sham group and SHA-3 group(p=0.010), control group and SHA-3 group(p=0.006) in the WBC count.
The Journal of The Korea Institute of Intelligent Transport Systems
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v.12
no.6
/
pp.116-126
/
2013
Weather factor to affect driver's driving environment are due to changes in weather conditions is caused rainfall, snowfall, fog etc and the reducing of road capacity and because deteriorating weather could be affect the headway and vehicle speed and the decrease in lane caused by an accident occurs that was analyzed that bad weather occur congestion greater than on the clear day. Bad weather to reduce of the driver's visibility was analysed the sensitive to changes in travel speed and traffic accident to appears high characteristics according to weather conditions on the expressway(higher speed) than general road. As a result, visibility have been determined to be important factor in changes of the highway traffic flow characteristics. Therefore, in this study, we selected traffic volume and travle speed that have a major impact on high-speed expressway basic segments as importance effect factor and we selected to subject of analysis among the Metropolitan Expressway that it could be obtained certain level of traffic volume data and we studied the changing of expressway traffic flow due to change visibility after the collecting of meteorological and traffic data. In order to perform this study, data collection and analysis methods were established through the existing literature and we had selected level of visibility of the expressway and we had performed the statistical verification. Finally, we had calculated the rate of change of expressway traffic flow characteristics due to visibility and we had come up with a way to apply at capacity and service level analysis.
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