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Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

  • Ma, Xiaojian;Wu, Chongde;Jun, Huang;Zhou, Rongqing;Shi, Bi
    • Journal of Microbiology and Biotechnology
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    • v.28 no.7
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    • pp.1168-1177
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    • 2018
  • The aim of this study was to investigate the microbial community of three tannery wastewater treatment plants (WWTPs) involved in nitrification by Illumina MiSeq sequencing. The results showed that highly diverse communities were present in tannery wastewater. A total of six phyla, including Proteobacteria (37-41%), Bacteroidetes (6.04-16.80), Planctomycetes (3.65-16.55), Chloroflexi (2.51-11.48), Actinobacteria (1.91-9.21), and Acidobacteria (3.04-6.20), were identified as the main phyla, and Proteobacteria dominated in all the samples. Within Proteobacteria, Beta-proteobacteria was the most abundant class, with the sequence percentages ranging from 9.66% to 17.44%. Analysis of the community at the genus level suggested that Thauera, Gp4, Ignavibacterium, Phycisphaera, and Arenimonas were the core genera shared by at least two tannery WWTPs. A detailed analysis of the abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) indicated that Nitrosospira, Nitrosomonas, and Nitrospira were the main AOB and NOB in tannery wastewater, respectively, which exhibited relatively high abundance in all samples. In addition, real-time quantitative PCR was conducted to validate the results by quantifying the abundance of the AOB and total bacteria, and similar results were obtained. Overall, the results presented in this study may provide new insights into our understanding of key microorganisms and the entire community of tannery wastewater and contribute to improving the nitrogen removal efficiency.

A Study on the Flow Characteristics of Oil-Water Separator for Marine Ship CFD (CFD에 의한 선박용 유수분리기의 유동특성에 관한 연구)

  • Kim, Byeong Jun;Kim, Sung Yoon;Roh, Chun Su;Lee, Young Ho
    • The KSFM Journal of Fluid Machinery
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    • v.19 no.4
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    • pp.48-53
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    • 2016
  • The centrifugal separator which uses gravity separation method for oil-water separation, rotating at high-speed, is one of the most commonly used device for controlling the amount of the oil in waste water collected in bilge. The IMO (International Maritime Organization) has set regulations, also known as MARPOL 73/78, for the prevention of marine pollution. In addition, DET NORSKE VERITAS (DNV) has set standards regarding the assignment of Environmental Class Notation, CLEAN or CLEAN DESIGN, of ships. One of the requirements for classification is that in addition to conforming to MARPOL 73/78, more stringent measures must be taken as well. One of these measures is to limit the oil concentration in bilge water to less than 5ppm. So in this study, an Oil-Water Separator (OWS) is used together with multiple separating plates as a filtration system to be used as an oil-water separation device. The OWS operates using centrifugal separation in which the mixture is separated by centrifugal forces. The main purpose of this paper is to present the OWS separation efficiency according to the rotation speed, mass-flow rate, the angle and the number of stacked layers of the laminated plate using Computational Fluid Dynamics (CFD). Improvements to the device will be investigated from these results.

Adsorption Characteristics of Methane and Carbon Dioxide in Zeolite with Flexible Framework (유연한 구조체를 가지는 제올라이트에서 메탄과 이산화탄소의 흡착 특성)

  • Yang Gon Seo
    • Clean Technology
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    • v.30 no.3
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    • pp.248-257
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    • 2024
  • Carbon dioxide is an undesired component of biogas and landfill gas. As a result, it needs to be removed from these mixtures in order to increase their heating value and reduce corrosion during treatment. Zeolites are a class of microporous materials that can be used as adsorbents for the separation of carbon dioxide from gas mixtures. In this work, the pure gas adsorption isotherms of methane and carbon dioxide and the selectivity of their mixture onto LTA-4A, FAU-13X and FAU-NaY adsorbents at temperatures of 273, 298 and 323 K and pressures up to 30 bars were calculated by the Monte Carlo method. Also, the influence of a flexible framework in a set of zeolites on the separation of methane and carbon dioxide was studied. Carbon dioxide adsorption onto the zeolites used in this work was more favorable than methane adsorption. The FAU-13X adsorbent had the highest adsorption capacity among the studied adsorbents. However, the selectivity of carbon dioxide over methane for LTA-4A was the highest. The adsorption capacities of a rigid framework were higher than those of a flexible framework. The influence of the framework flexibility in FAU on adsorption capacity was small. In contrast, its influence on selectivity seemed to be much larger.

Trace level analysis of Pb in plasma by inductively coupled plasma/mass spectrometry (유도결합플라즈마 질량분석법을 이용한 혈장 중 극미량 납 분석)

  • Lee, Sung-Bae;Yang, Jeong-Sun;Choi, Sung-Bong;Shin, Ho-Sang
    • Analytical Science and Technology
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    • v.25 no.3
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    • pp.190-196
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    • 2012
  • The human exposure of lead has usually detected the amount of lead in the whole blood, however, this method has a shortcoming to give the information on the short-term exposure to lead. In that sense, it is desirable to estimates the level of lead in plasma to draw the chronic bio-marker of lead exposure even though it is difficult to measure lead of several ng/L. An inductively coupled plasma-mass spectrometry (ICP-MS) method was developed for determining lead in plasma as the chronic bio-marker of lead of workers. To minimize the contamination of lead from the environment, we constructed class 1,000 clean room and compared the amount of floating dust before and after the operation of the clean room. The limit of detection (LOD) and the limit of quantification (LOQ) of lead in fetal bovine serum were 4.3 ng/L and 12.2 ng/L by NIOSH method (statistical calculation method) and 7.0 ng/L and 22.1 ng/L by signal/noise ratio, respectively. The accuracy was in a range of 92.3-101.3%, and the precision of the assay was less than 4% in the samples spiked in the concentration of 20 ng/L and 2,000 ng/L. The method was simple, reproducible and sensitive enough to permit reliable analysis of lead to the ng/L level in plasma and/or serum. The method was also useful for the biological monitoring of chronic exposure to lead.

MICROTENSILE BOND STRENGTH ACCORDING TO DIFFERENT DENTIN WALL POSITION IN CLASS I CAVITY OF PRIMARY MOLAR (유구치 1급 와동에서 와동벽 위치에 따른 microtensile bond strength 비교 연구)

  • Lee, Hyeon-Heon;Jung, Tae-Ryun;Kim, Jung-Wook;Jang, Ki-Taeg
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.4
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    • pp.693-698
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    • 2006
  • In Class I cavity, the highest C-factor could be obtained and it means the highest polymerization shrinkage stress. In this study, high C-factor model was designed. The pulpose of present study was to determine differences of Microtensile bond strength (MTBS) of class I cavity pulpal and axial wall specimens in primary molar. Twenty clean mandibular 2nd primary molars were randomly divided into two groups Different composite Resins (Filtek Z250, 3M ESPE & Filtek Supreme, 3M ESPE) were bulk filled and photo cured. Axial wall specimens and pulpal specimens were prepared at the same teeth, All specimens were divided into 4 groups and MTBS were evaluated. Group ZP : Filtek Z250-Pulpal wall Group ZA : Filtek Z250-Axial wall Group SP : Filtek Supreme - Pulpal wall Group SA : Filtek Supreme - Axial wall The results were as follows: 1. Mean MTBS of ZP & ZA and SP & SA were significantly different(p<.001). 2. There was no significant difference between MTBS of ZP & SP and ZA & SA.

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Assessment of Bacteriological Safety of the Seawater and Ark shell (Scapharca subcrenata) in Yeoja Bay, Korea (전남 여자만 해수 및 새꼬막(Scapharca subcrenata)의 세균학적 위생 평가)

  • SHIN, SoonBum;OH, Eun Gyoung;JEONG, Sang Hyeon;LEE, Hee Jung;KIM, Yeon Kye;LEE, Tae Seek
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.5
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    • pp.1435-1443
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    • 2016
  • This study evaluated the sanitary state of seawater and shellfish in Yeoja bay from January 2014 to June 2016. The sampling stations for sanitary survey in Yeoja Bay was composed of 50 stations for seawater and 4 stations for ark shell and the samples have been collected monthly at a stated date. The total and fecal coliforms in seawater and Escherichia coli (E. coli) in shellfish were investigated as sanitary indicator bacteria, respectively. The geometric mean and the estimated 90th percentile ranges of total and fecal coliforms for seawater were <1.8-3.9, <1.8-2.6 and <1.8-29.7, <1.8-9.3 MPN/100 mL, respectively. The range of E. coli levels for ark shell were <20~330 MPN/100 g. The sanitary state in Yeoja bay was evaluated clean area according Korea criteria and conditionally approved area according US criteria and Class B area according EU criteria.

Development of a Mask Aligner Simulator for Education (노광 장치 시뮬레이터 개발)

  • Kim, Dae Jeong;Park, Yun Jeong;Jung, Taeho
    • Journal of the Korea Society for Simulation
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    • v.26 no.4
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    • pp.43-49
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    • 2017
  • With the advances in and expansion of the semiconductor and display businesses in Korea the demand of the engineers in such fields is increasing. Keeping pace with the trend, the semiconductor courses in undergraduate not only include the newest technologies in addition to the fundamental theories but fabrication related technologies as well in order to produce engineers with practical knowledge. However, since semiconductor fabrication requires expensive equipment and materials in a clean room, laboratory class can't be provided in undergraduate. To overcome this limitation actual fabrication processes are recorded in video and played in class. In addition, 3D visualization of fabrication processes can be used.

Transcriptome analysis of the livers of ducklings hatched normally and with assistance

  • Liu, Yali;He, Shishan;Zeng, Tao;Du, Xue;Shen, Junda;Zhao, Ayong;Lu, Lizhi
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.6
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    • pp.773-780
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    • 2017
  • Objective: "Hatchability" is an important economic trait in domestic poultry. Studies on poultry hatchability focus mainly on the genetic background, egg quality, and incubation conditions, whereas the molecular mechanisms behind the phenomenon that some ducklings failed to break their eggshells are poorly understood. Methods: In this study, the transcriptional differences between the livers of normally hatched and assisted ducklings were systematically analyzed. Results: The results showed that the clean reads were de novo assembled into 161,804 and 159,083 unigenes (${\geq}200-bp$ long) by using Trinity, with an average length of 1,206 bp and 882 bp, respectively. The defined criteria of the absolute value of log2 fold-change ${\geq}1$ and false discovery rate${\leq}0.05$ were differentially expressed and were significant. As a result, 1,629 unigenes were identified, the assisted ducklings showed 510 significantly upregulated and 1,119 significantly down-regulated unigenes. In general, the metabolic rate in the livers of the assisted ducklings was lower than that in the normal ducklings; however, compared to normal ducklings, glucose-6-phosphatase and ATP synthase subunit alpha 1 associated with energy metabolism were significantly upregulated in the assisted group. The genes involved in immune defense such as major histocompatibility complex (MHC) class I antigen alpha chain and MHC class II beta chain 1 were downregulated in the assisted ducklings. Conclusion: These data provide abundant sequence resources for studying the functional genome of the livers in ducks and other poultry. In addition, our study provided insight into the molecular mechanism by which the phenomenon of weak embryos is regulated.

Research and Development & Commercial Deployment Status for Coal Gasification Technology - Mainly from GTC 2010 (2010 가스화 기술 컨퍼런스를 중심으로 살펴본 석탄 가스화 기술의 개발 동향 및 상업화 전개 현황)

  • Lee, Jin-Wook;Yoo, Young-Don;Yun, Yong-Seung
    • Journal of Energy Engineering
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    • v.20 no.2
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    • pp.123-142
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    • 2011
  • Coal Gasification Technology is one of the best alternatives among clean fossil fuel utilization. Major technology holding companies are devoting their efforts to develop more advanced technology to dominate the market in advance because of its importance on the applications such as IGCC, CTL, coal to SNG, various chemicals and so on. Japan and China, as well as America and European countries, have developed couple of thousands ton/day-class coal gasification technology. However, our gasification technology remains in the development stage with couple of ton/day-class pilot plant. So, we should be interested in developing this technology considering that we are heavily dependent of coal energy. In this paper, we summarized the trend and status of coal gasification technology development and commercial deployment of major technology holding companies mainly from the presentation materials of 'Gasification Technology Conference 2010', which is the biggest place of information exchange for recent coal gasification technology.

A Study for Development and Characteristics of Electrostatic Eliminator Suitable for the Super Clean Room Less than Class 100(I) (공기 청정지역(Class 100 이하)에 적합한 정전기 제거장치의 개발 및 특성에 관한 연구(I) - LCD 제조 공정을 중심으로 -)

  • Jung, Yong-Chul;Park, Hoon-Kyu;Lee, Dong-Hoon
    • Journal of the Korean Society of Safety
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    • v.21 no.4 s.76
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    • pp.60-65
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    • 2006
  • It is a well known fact that LCD is a central part of the IT industry which is important in the present and the future. But the biggest problem of LCD manufacturing is maintaining a cleaning room environment and administration. Therefore the purpose of this study is to first, prevent the yield depreciation and damage of products, and second, protect the worker ftom accidental electrostatic discharge during LCD manufacture. The soft x-ray ionizer is a type of electrostatic reducer device. It protects against electrostatic discharge in the cleaning room environment and is a necessary environmental factor during LCD production. The positive aspects of the soft x-ray are its shorter time and wider angle of exposure. But the negative aspect of the soft x-ray is its need for several shielding of protection from the harmful x-ray exposure. On this study, the development of the Air Nozzle-type ionizer to amend and refine some problems. For example, examined the electrostatic reduce device of a soft x-ray type and discovered the ion did not go inside well. also workers to be free from danger. An Air Nozzle-type ionizer is comprised of soft x-ray radiation and ionized air production. Air is injected through the nozzle after being ionized from radiation. It supplies air keeping the same pressure into the end foundation of ion production. The soft x-ray is the structure which radiates ionized air through the nozzle (21 holes) having micro holes of the ionizable radiation after ionizing the inside air by the ion production. A worker does not need a cover to protect against x-rays and the Air Nozzle-type ionizer is easy to set up and is more effective at eliminating electrostatic.