• Bulletin of the Korean Chemical Society
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• v.23 no.8
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• pp.1116-1119
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• 2002

Monoclonal antibodies have been generated against a generic hapten, ο,ο-diethyl ο-(5-carboxy-2-fluorophenyl) phosphorothioate, for the determination of organophosphorus (OP) pesticides in a class-specific manner. In an indirect competitive enzyme-linked immunosorbent assay (ELISA) format, employing a heterologous coating antigen, these monoclonal antibodies showed desirable properties for use in the class-specific determination, i.e., broad specificity and high sensitivity. The IC50 values of four commonly used ο,ο-diethyl OP pesticides were fairly uniform ranging from 0.1 to 0.3 ㎛/mL. The IC50 values of three ο,ο-dimethyl derivatives were between 0.3 and 1.4 ㎛/mL. These values, together with the limits of detection (LOD), were better, in terms of the specificity and sensitivity, compared with the values obtained previously with polyclonal antibodies.

• Bulletin of the Korean Chemical Society
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• v.24 no.1
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• pp.11-12
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• 2003

• Korean Journal of Clinical Laboratory Science
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• v.40 no.2
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• pp.94-97
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• 2008

The human leukocyte antigen (HLA) is the name of the major histocompatibility complex (MCH) in humans. The superlocus contains a large number of genes related to immune system function in humans. This group of genes resides on chromosome 6. and encode cell surface antigen-presenting proteins and many other genes. HLA class I antigen (A, B & C) present peptides from inside the cell. These peptides are produced from digested proteins that are broken down in the lysozymes. Most expressed HLA loci exhibit a remarkable degree of allelic polymorphism, which derives from sequence differences predominantly localized to discrete hypervariable regions of the amino terminal domain of the molecule. In this sutdy, the HLA-A genotypes were determined in twenty students unrelated koreans using the PCR-SSP (Polymerase Chain Reaction-Sequence Specific Primer) technique. Several specific primer pairs in assigning the HLA-A gene were used (A*0201, A*33, A*2401). The results of PCR-SSP, the HLA-A*0201 primer was detected eleven (55%), the HLA-A*33 were detected seven (35%) and the HLA-A*2401 were detected seven (35%). This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-A genotypes.

• Journal of Aerospace System Engineering
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• v.16 no.3
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• pp.52-62
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• 2022

In the SORA methodology developed for the operational risk assessment of a specific category of operation of a UAS, the ground and the air risk levels are determined, and a SAIL indicating the level of assurance and integrity for the corresponding risk is assigned, and accordingly, the operational safety level for the proposed operation. Objectives should be demonstrated at an appropriate level of robustness. Because of the nature of the specific category of operation, people on the ground are the first risk subjects to be considered. The resulting ground risk class plays an important role in the allocation of SAIL. In this paper, the impact on SAIL and OSO according to the final risk level and the reduction of the level through the determination of the ground risk level and the application of mitigation measures among risk assessments for specific categories of UAV operation was investigated.

• Journal of Korean Tunnelling and Underground Space Association
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• v.3 no.1
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• pp.21-26
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• 2001

Till now a lot of studies has been performed to increase the efficiency of tunnel blasting. Nevertheless there are still uncertainties of input parameter to determine the specific charge. In order to solve this problem, the rock types and the charges of 17 road tunnel sites were analyzed. As a result of these analyses an empirical formula depending on rock type and charge was developed. Through this formula rational tunnel blasting will be designed by quantitative method rather than by assumption.

• Computers and Concrete
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• v.1 no.3
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• pp.303-324
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• 2004

The Component-Based Software Development (CBSD) has established itself as a sound paradigm in the software engineering discipline and has gained wide spread acceptance in the industry. The CBSD relies on the availability of standard software components for encapsulation of specific functionality. This paper presents the framework for the development of a software component for the design of general member cross-sections. The proposed component can be used in component-based structural engineering software or as a stand-alone program developed around the component. This paper describes the use-case scenarios for the component, its design patterns, object models, class hierarchy, the integrated and unified handling of cross-section behavior and implementation issue. It is expected that a component developed using the proposed patterns and model can be used in analysis, design and detailing packages to handle reinforced concrete, partially prestressed concrete, steel-concrete composite and steel sections. The component can provide the entire response parameters of the cross section including determination of geometric properties, elastic stresses, flexural capacity, moment-curvature, and ductility ratios. The component can also be used as the main computational engine for stand-alone section design software. The component can be further extended to handle the retrofitting and strengthening of cross-sections, shear and torsional response, determination of fire-damage parameters, etc.

• Journal of Korean Home Economics Education Association
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• v.2 no.1
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• pp.123-142
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• 1990

The aim of this study is to apply lesson design of high school home economics to model which consists of six steps of instructional procedure among various models for instructional design, Through thesis, central content of each step was written as follows; 1) At the step of establishment of terminal instructional objectives, educational objectives, terminal instructional objectives was analyzed and specific instructional objectives was extracted. 3) At the step of diagnosis of entering behavior, ability of prior learning and ability of preliminary learning were evaluated, which was used as establishing instructional strategy and doing individual guidance. 4)At the step of determining instructional strategy, instructional strategy should be extracted by considering instructional event which is adequate to content of instruction. Instructional strategy consists of five main factor, factors, those are, activity of introduction of instruction, presentation of content of instruction and information, participation of learner and learning activity, identification of learning result, and subsequent treatment after evaluation. 5)Specific activity at the step of selection and development of instructional media consists of reidentification of instructional objectives determination of learning type, drawing up instructional media analysis sheet, synthesis of selected media, and explanatory note of selected media. In order to increase efficiency of instruction at the step of determining instructional strategy, this step should be considered simultaneously. 6)The step of try-out and evaluation of instructional design should be efficient when designed instruction was inputted at class after evaluation of each step of instructional design.

• Biomedical Science Letters
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• v.2 no.2
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• pp.223-229
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• 1996

HLA-B27 gene, one of the HLA-class I molecule, is strongly associated with ankylosing spondylitis. It has been most frequently used as a disease-correlated HLA gene by clinicians. In most laboratories, conventional HLA-B27 typing is still performed by cell cytotoxicity tests or fluorescence serology with specific antibodies. In this study, DNA typing method for HLA-B27 was developed by using group specific Polymerase Chain Reaction (PCR). Four HLA-B27 cell lines (HOM-2, JESTHOM, WT24 and BTB) and fifty six B27 Korean individuals defined by serology were used. The results of control cell and B-27 positive individual samples were correlated well with the data which was performed by serological method. All of B27 positive PCR products gave positive signals on Southern blot hybridization with B27 specific probe. This study shows that the HLA-B27 DNA typing is a relatively simple, fast and practical tool for the determination of the HLA-B27 gene in routine clinical laboratory work.

• English Language & Literature Teaching
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• v.17 no.4
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• pp.97-121
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• 2011

This study aims to examine how motivational constructs are interrelated with social, context-specific factors and, as a result, contribute to L2 writing achievement within the framework of self-determination theory. The data consisted of 67 Korean college students' questionnaire responses, final scores in an on-offline blended writing course, and qualitative interviews with 5 students. In the descriptive and the correlation analyses, the participants' extrinsic motivation was found higher than intrinsic motivation, with low amotivation. Among social factors, immersion environment, foreign instructor, and peer comparison marked high scores, whereas Korean instructor and online material gained low scores. Those contextual factors were interrelated with each other, such that the immersion factor correlated significantly with Korean instructor and peer comparison. Extrinsic and intrinsic motivational subscales engendered strong correlations with the high-scored social factors, i.e., immersion, foreign instructor, and peer comparison, which were also closely interrelated with L2 writing achievement. The findings illuminate intricate workings of motivation in its effects on L2 achievement and corroborate the roles of contextual factors. The effect of motivational subscales on achievement may be valid through interplay with some social factors. The dynamics of motivation is discussed for pedagogical applications.

• The KIPS Transactions:PartB
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• v.12B no.7 s.103
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• pp.829-840
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• 2005

Word Sense Disambiguation(WSD) is one of the most difficult problem in Korean information processing. We propose a WSD model with the capability to filter semantic information using the specific characteristics in dictionary dictions, and nth added information, useful to sense determination, such as statistical, distance and case information. we propose a model, which can resolve the issues resulting from the scarcity of semantic information data based on the word hierarchy system (thesaurus) developed by Ulsan University's UOU Word Intelligent Network, a dictionary-based toxicological database. Among the WSD models elaborated by this study, the one using statistical information, distance and case information along with the thesaurus (hereinafter referred to as 'SDJ-X model') performed the best. In an experiment conducted on the sense-tagged corpus consisting of 1,500,000 eojeols, provided by the Sejong project, the SDJ-X model recorded improvements over the maximum frequency word sense determination (maximum frequency determination, MFC, accuracy baseline) of $18.87\%$ ($21.73\%$ for nouns and inter-eojeot distance weights by $10.49\%$ ($8.84\%$ for nouns, $11.51\%$ for verbs). Finally, the accuracy level of the SDJ-X model was higher than that recorded by the model using only statistical information, distance and case information, without the thesaurus by a margin of $6.12\%$ ($5.29\%$ for nouns, $6.64\%$ for verbs).