• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3977-3982
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• 2012

Inhibitors of histone deacetylase activity are emerging as a potentially important new class of anticancer agents. In this study, we assessed the anticancer effects of valproic acid (VPA) on ovarian cancer in vitro and in vivo. Cultured SKOV3 cells were treated by VPA with different concentrations and time, then the effects on cell growth, cell cycle, apoptosis, and related events were investigated. A human ovarian cancer model transplanted subcutaneously in nude mice was established, and the efficacy of VPA used alone and in combination with diammine dichloroplatinum (DDP) to inhibit the growth of tumors was also assessed. Proliferation of SKOV3 cells was inhibited by VPA in a dose and time dependent fashion. The cell cycle distribution changed one treatment with VPA, with decrease in the number of S-phase cells and increase in G1-phase. VPA could significantly inhibit the growth of the epithelial ovarian cancer SKOV3 cells in vivo without toxic side effects. Treatment with VPA combined with DDP demonstrated enhanced anticancer effects. The result of flow cytometry (FCM) indicated that after VPA in vitro and in vivo, the expression of E-cadherin was increased whereas vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were decreased. This study suggests that VPA could be a novel attractive agent for treatment of ovarian cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1715-1717
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• 2014

The overall incidence and mortality of renal cell carcinoma (RCC), the most common kidney cancer, are steadily increasing for reasons that are not fully explained. Our aim was to explore the expression of membrane MHC class I chain-related gene A (mMICA) in human RCC cell lines and tissue specimens, and to determine expression of soluble MICA (sMICA) in serum of patients with renal cell carcinoma, we used flow cytometry (FCM) and immunohistochemistry as well as an enzyme linked immunosorbent assay (ELISA). The results showed that percentage of mMICA expression was significantly increased in human kidney cancer tissues and RCC cell lines (786-O and Ketr-3) than that in healthy adults and human embryonic kidney 293 (HEK293) cell line individuality (P<0.05). sMICA content in healthy adults was negative, but in renal cancer patients was significantly elevated (P<0.05). Our research showed that high expression of MICA in human kidney cancer, this results show that MICA might serve as potential tumor-associated antigen (TAA) in RCC.

• 대한조선학회논문집
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• 제50권3호
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• pp.145-152
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• 2013

Recently, with oil price jumping and environmental issues, Green ship is paid deep attention to by ship owner, operator, builder, class and government. Fuel efficiency and reduction of $CO_2$ emissions are expected to have a strong influence on the design and operation of merchant ships. Many ship owners and operators are seeking the more economic method by the best operating route and the application of reliable and effective energy saving devices. With the Energy Efficiency Design Index (EEDI) in 2013 attention will more than ever be focused at achieving maximum fuel economy in the hydrodynamic design of hull forms, their appendages and propellers. IMO requirements for $CO_2$ emission for ships will now be implemented for vessels ordered from 1st January 2013. So far, a lot of new idea and patents have been proposed, tested, claimed and applied for various kinds of ship type. This paper shows numerical and experimental work related to a study on a energy saving devices particularly for fuller ship such as merchant vessel of Tanker and Bulker. From the bare hull wake measurements, typical upper/lower asymmetry of hull wake at the propeller disk was found. The pre-swirl duct have been designed and reviewed to recover the loss of propeller running in that condition. The general function of the pre-swirl duct was set to work against this asymmetry of wake and generate pre-swirled flow into the propeller against the propeller rotating direction.

• 대한한방부인과학회지
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• 제23권2호
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• pp.57-70
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• 2010

Purpose: In this study, we investigated the effects of GDB(Gidaebang) on the immune response to establish the treatment mechanism of vaginitis. Methods: We examined the effects of GDB on the DCs(Dendritic cells) phenotypic and functional maturation. iDCs were cultured in the presence of GM-CSF and the generated iDCs were respectively stimulated by GDB or LPS as the control group for 24 hours. To evaluate the DCs phenotypic and functional maturation, we used flow cytometric analysis, RT-PCR and ELISA. Results: 1. GDB upregulated the expression of class II MHC and CD40 on DCs. 2. GDB upregulated the expression of CD80 and CD86 on DCs. 3. GDB induced cytokine IL-12 production and mRNA expression in DCs. Conclusion: These results suggest that GDB is able to improve the antigen-presenting capacity of DCs through the upregulation of their maturation, and might induce proliferation of T cells. In conclusion, this immunomodulatory properties of GDB may be useful in the treatment of vaginitis.

• 한국동물위생학회지
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• 제30권1호
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• pp.51-66
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• 2007

The purpose of this study was to evaluate the effect of a subsequent infection of porcine reproductive and respiratory syndrome(PRRS) virus to pigs with A pleuropneumonia. Twenty three 7-week-old commercial pigs were infected intratracheally with PRRS virus and/or A pleuropneumoniae serotype 5. Serum antibody titers were examined by an enzyme-linked immunosorbent assay(ELISA) and proportion of porcine leukocyte subpopulations in peripheral blood was examined by flow cytometry. In this experiment, antibodies against PRRS virus and A pleuropneumoniae were detected at 2 weeks and 1 week postinfection and the number of antibody positive pigs were gradually increased. And in proportion to leukocyte subpopulations in peripheral blood of pigs infected with A pleuropneumoniae compared with pigs administrated with saline, the proportion of PoCD4 and N cells were increased(P<0.1). Furthermore, in proportion to leukocyte subpopulations in peripheral blood of pigs infected with PRRS virus followed by A pleuropneumoniae compared with pigs administrated with saline, the proportion of MHC class II, PoCD4 and B cells were significantly increased(P<0.1). The results indicated that dual infection with PRRS virus and A pleuropneumoniae induced the stronger immune responses associated with macrophages and Th cells in pigs than single infection with PRRS virus or A pleuropneumoniae.

• 한국화재소방학회논문지
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• 제25권3호
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• pp.51-56
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• 2011

도로터널에서의 미분무수 소화시스템의 화재진압 특성을 조사하기 위해 실물 터널 화재 실험을 수행하였다. 적용된 소화시스템은 압력이 3.5 bar인 저압 물분무 소화설비와 60 bar인 고압 미분무수 소화시스템이다. 미분무수 소화시스템은 물분무 소화시스템의 1/6 만큼의 소화용수량을 사용한다. 화원 (fire source)은 실물 승용차와 유류화재를 모사한 화원면적 $1.4m^2$의 헵탄 연료 팬 화재로 구성하였다. 터널 내의 환기조건을 구현하기 위해 실물모형 터널의 한쪽 끝단에 유속(0.9~3.8 m/sec 범위) 발생장치를 설치하였으며, 화원에서 하류 방향으로 터널 내 온도분포는 K-type 열전대 트리를 사용하여 측정하였다. 실험 결과 고압 미분무수 소화시스템은 B급 화재의 경우 저압 물분무 시스템과 동등한 수준의 냉각효과를 보였다.

• 정보학연구
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• 제5권1호
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• pp.69-77
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• 2002

블루투스는 차세대 근거리 무선통신 기술로 각광받고 있으며, 프로토콜 스택은 블루투스 응용 시스템을 위한 다양한 서비스를 제공한다 블루투스 규격은 개방된 세계적인 규격으로 완전한 시스템을 정의하고 있지만 프로토콜 스택은 개발자에 따라 다른 방법으로 하드웨어와 소프트웨어의 역할을 기능성 중심으로 독자적으로 분할하여 설계 및 구현이 가능하다. 이는 프로토콜 스택의 기능적인 면에서 두 계층에서의 구현 방법이 다르다는 것을 의미한다. 본 논문에서는 프로토콜 스택 개발의 첫 단계로 소프트웨어 계층의 최하위 계층인 HCI 계층의 개발에 중점을 두었다. 그 결과로서 HCI 계층의 기능적 역할 분담과 흐름제어 기능 및 패킷들 사이의 관계를 설계 및 구현하였다. 실험결과는 다른 블루투스 장치들과의 연결 과정을 보여주고 있으며, 이것은 블루투스 모듈 사이에서의 데이터 통신이 적절한 운영을 하고 있음을 증명한다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3471-3476
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• 2012

Background and Objective: Histone deacetylase (HDAC) inhibitors represent a promising class of potential anticancer agents for treatment of human malignancies. In this study, we investigated the effect of trichostatin A (TSA), one such HDAC inhibitor, in combination with docetaxel (TXT), a cytotoxic chemotherapy agent or erlotinib, a novel molecular target therapy drug, on lung cancer A549 cells. Methods: A549 cells were treated with TXT, erlotinib alone or in combination with TSA, respectively. Cell viability, apoptosis, and cell cycle distribution were evaluated using MTT (3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide) assay, Hochst33258 staining and flow cytometry. Moreover, immunofluorescent staining and Western blot analysis were employed to examine alterations of ${\alpha}$-tubulin, heat shock protein 90 (hsp90), epidermal growth factor receptor (EGFR), and caspase-3 in response to the different exogenous stimuli. Results: Compared with single-agent treatment, co-treatment of A549 cells with TSA/TXT or TSA/erlotinib synergistically inhibited cell proliferation, induced apoptosis, and caused cell cycle delay at the $G_2/M$ transition. Treatment with TSA/TXT or TSA/erlotinib led to a significant increase of cleaved caspase-3 expression, also resulting in elevated acetylation of ${\alpha}$-tubulin or hsp90 and decreased expression of EGFR, which was negatively associated with the level of acetylated hsp90. Conclusions: Synergistic anti-tumor effects are observed between TXT or erlotinib and TSA on lung cancer cells. Such combinations may provide a more effective strategy for treating human lung cancer.

• 한국콘텐츠학회논문지
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• 제16권7호
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• pp.504-512
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• 2016

본 연구는 뮤지컬 배우의 공연 후 배역 투사와 그 후유증 양태를 파악하여 제도적인 치유 시스템을 모색하기 위해 실시되었다. 우리나라 정상급 남녀 뮤지컬 배우 22명을 상대로 심층 인터뷰를 하여 분석한 결과, 배우들은 대부분 공연 후 1개월 이상 장기간 배역 투사에 시달리며 우울증 등의 후유증으로 고통 받고 있으며, 치유도 전문가 상담을 회피한 채 자기진단에 따라 기분전환에 의존하고 있는 것으로 나타났다. 투사는 극중 주인공, 부정적 현실적 캐릭터 연기, 격한 감정을 표출할 때 특히 두드러졌다. 연구 결과는 배우들의 생생한 제언처럼 케이컬처 시대의 수준에 맞게 배우가 출연 전후 배우 친화적인 전문가의 상담 치료를 받도록 입법을 하고 재원을 조성해 민간에 배우전문 케어센터를 구축해야 한다는 점을 암시한다.

• IMMUNE NETWORK
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• 제10권6호
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• pp.188-197
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• 2010

Background: Lichen-derived glucans have been known to stimulate the functions of immune cells. However, immunostimulatory activity of glucan obtained from edible lichen, Umbilicaria esculenta, has not been reported. Thus we evaluated the phenotype and functional maturation of dendritic cells (DCs) following treatment of extracted glucan (PUE). Methods: The phenotypic and functional maturation of PUE-treated DCs was assessed by flow cytometric analysis and cytokine production, respectively. PUE-treated DCs was also used for mixed leukocyte reaction to evaluate T cell-priming capacity. Finally we detected the activation of MAPK and NF-${\kappa}B$ by immunoblot. Results: Phenotypic maturation of DCs was shown by the elevated expressions of CD40, CD80, CD86, and MHC class I/II molecules. Functional activation of DCs was proved by increased cytokine production of IL-12, IL-$1{\beta}$, TNF-${\alpha}$, and IFN-${\alpha}/{\beta}$, decreased endocytosis, and enhanced proliferation of allogenic T cells. Polymyxin B, specific inhibitor of lipopolysaccharide (LPS), did not affect PUE activity, which suggested that PUE was free of LPS contamination. As a mechanism of action, PUE increased phosphorylation of ERK, JNK, and p38 MAPKs, and enhanced nuclear translocation of NF-${\kappa}B$ p50/p65 in DCs. Conclusion: These results indicate that PUE induced DC maturation via MAPK and NF-${\kappa}B$ signaling pathways.