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Effect of porcine reproductive and respiratory syndrome virus on pigs with pleuropneumonia - Studies on serology and proportion of porcine subpopulation of peripheral blood -  

Jeong, Hyun-Kyu (Dodram Pig Farmer's Co-operative)
Park, Yong-Ho (Department of Microbiology, College of Veterinary Medicine, Seoul National University)
Han, Jeong-Hee (Department of Veterinary Pathology, School of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University)
Publication Information
Korean Journal of Veterinary Service / v.30, no.1, 2007 , pp. 51-66 More about this Journal
Abstract
The purpose of this study was to evaluate the effect of a subsequent infection of porcine reproductive and respiratory syndrome(PRRS) virus to pigs with A pleuropneumonia. Twenty three 7-week-old commercial pigs were infected intratracheally with PRRS virus and/or A pleuropneumoniae serotype 5. Serum antibody titers were examined by an enzyme-linked immunosorbent assay(ELISA) and proportion of porcine leukocyte subpopulations in peripheral blood was examined by flow cytometry. In this experiment, antibodies against PRRS virus and A pleuropneumoniae were detected at 2 weeks and 1 week postinfection and the number of antibody positive pigs were gradually increased. And in proportion to leukocyte subpopulations in peripheral blood of pigs infected with A pleuropneumoniae compared with pigs administrated with saline, the proportion of PoCD4 and N cells were increased(P<0.1). Furthermore, in proportion to leukocyte subpopulations in peripheral blood of pigs infected with PRRS virus followed by A pleuropneumoniae compared with pigs administrated with saline, the proportion of MHC class II, PoCD4 and B cells were significantly increased(P<0.1). The results indicated that dual infection with PRRS virus and A pleuropneumoniae induced the stronger immune responses associated with macrophages and Th cells in pigs than single infection with PRRS virus or A pleuropneumoniae.
Keywords
PRRS virus; A pleuropneumoniae; dual infections; ELISA; porcine leukocyte subpopulations;
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1 Bille N, Larsen JI, Sevendsen J. 1975. Preweaning mortality in pigs. 6. Incidence and causes of pneumonia. Nord Vet Med 27: 482-495
2 Benfield DA, Hais L, Nelson E, et al. 1992. Properties of PRRS virus isolate ATCC VR-2332 in the United States and preliminary characterization of a monoclonal antibody to this virus. Am Swine Pract News 4: 19-21
3 Pijoan C and Molitor T. 1992. Viral-bacterial interactions in respiratory disease of pigs. In Proc of 15th IPVS Con 29-31
4 Pol JMA, van Leengoed LA, Stockhofe N, et al. 1997. Dual infections of PRRSV /influenza or PRRSV/Actinobacillus pleuropneumoniae in the respiratory tract. Vet Microbiol 55(1-4). 259-264   DOI   ScienceOn
5 Lombin LH, Rosendal S, Mitchell WR. 1982. Evaluation of the complement fixation test for the diagnosis of pleuropneumonia of swine caused by Haemophilus pleuropneumoniae. Can J Camp Med 46: 109-114
6 Brandreth SR, Smith IM. 1985. Prevalence of pig herds affected by pleuropneumonia associated with Haemophilus pleuropneumoniae in eastern England. Vet Res 117: 143-147
7 Tonegawa S. 1985. The molecules of the immune system. Sci Am 253: 122-131
8 Kim HS, Kwang J, Yoon IJ, et al. 1993. Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Arch Virol 133: 477-483   DOI
9 Shimizu M, Yamada S, Murakami Y, et al. 1994. Isolation of porcine reprodutive and respiratory syndorome(PRRS) virus from Heko-Heko disease of pigs. J Vet Med Sci 56: 389-391   DOI
10 Pescovitz MD, Lunney JK and Sachs DH. 1984. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol 133: 368-375
11 Zhou Y, Barghusen S, Choi C,et al. 1992. Effect of PRRS virus infection on leucocyte populations in the peripheral blood and on cytokine expression in alveolar macrophages of growing pigs. Am Assoc Swine Pract Newsletter 4;28
12 Goverman J, Hunkapiller T and Hood L. 1986. A speculative view of the multicomponent nature of T cell antigen recognition. Cell 45: 475-484   DOI   ScienceOn
13 Paliard X, Malefijt RW, de Vries JE, Spits H. 1988. Interaleukin-4 mediates CD8 induction on human CD4+ T-cell clones. Nature 335: 642-644   DOI   ScienceOn
14 ten Berge RJ, Sauerwein HP, Yong SL and Schellekens PTHA. 1984. Administration of prednisolone in vivo affects the ratio of OKT4/OKT8 and the LDH-isoenzyme pattern of human T lymphocytes. Clin Immunol 30: 91-103
15 Westermann J and Pabst R. 1990. Lymphocyte subsets in the blood : a diagnostic window on the lymphoid system. Immunol Today 11: 406-410   DOI   ScienceOn
16 Zeman DH. 1995. Concurrent respiratory infections in 221 cases of PRRS virus pneumonia : 1992' [1994. Swine Heal Pro 4(3): 143-145
17 Thacker B, Mulks M, Yamini B et al. 1987. Clinical, immunological, hematological, microbiological and pathological evaluation of percutaneous intratracheal injection of Haemophilus pleuropneumoniae challenge model. Int Pig Vet Sci 69-79
18 Yagahasi T, Nunoya , Mistui T, et al. 1984. Effect of Mycoplasma hyopneumoniae infection on development of Haemophilus pleuropneumoniae pneumonia in pigs. Jpn J Vet Sci 46: 705-713   DOI
19 van der Valk P and Herman CJ. 1987. Biology of disease. Leukocyte functions. Lab Invest 57: 127-137
20 이승욱. 1998. 통계학의 이해. 자유아카데미. 125
21 Davis WC, Hamilton MJ and Park YH. 1990. Ruminant leucocyte differentiation molecules In : Barta. MHC, differentiation antigen, and cytokines in animal and birds. Monographs in Animal Immunol. Blacksburg, VA : BAR-1, AB, Inc 47-70
22 Davis WC, Marusic S, Lewin HA, et al. 1987. The development and analysis of species specific and cross reactive monoclonal antibodies to leucocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet Immunol Immunopathol 15: 337-376   DOI   ScienceOn
23 Galina L, Pijoan C, Sitjar M, et al. 1994. Interaction between Streptococcus suis serotype 2 and porcine reproductive and respiratory syndrome virus in specific pathogen-free piglets. Vet Rec 134(3). 60-64   DOI   ScienceOn
24 Yoon IJ, Joo HS, Christianson WT. 1992. Isolation of a cytopatic virus from weak-born pigs of farms with a history of swine infertility and respiratory syndrome. J Vet Diagn Invest 4. 139-143   DOI
25 Hammerbeg C and Schurig GG. 1986. Characterization of monoclonal antibodies directed against swine leukocytes. Vet Immunol. Immunopathol 11: 107-121   DOI   ScienceOn
26 문진산, 박용호, 정석찬 등. 1996. 돼지 Mycoplasma hyopneumoniae와 Pasteurella multocida의 정제항원과 Quill A를 이용한 ISCOM 접종시 백혈구 아집단 분포율 및 방어효과. 대한미생물학회지 31(1) 45-54
27 Chinsakchai S, Molitor TW. 1994. Immunology of pseudorabies virus infection in swine. Vet Immunol Immunopathol 1994. 43: 107-116   DOI   ScienceOn
28 Johnson H Jr, Lunney JK, Sachs DH, Flye MW. 1980. Preparation and characterization of an antiserum specific for T cells of pigs. Transplantation 29: 477-483   DOI   ScienceOn
29 Salmon H. 1979. Surface markers of porcine lymphocytes and distribution in various lymphoid organs. Int Archs Allergy Appl. Immunol 60: 262-274   DOI
30 Carbone AM, Marrack P and Kappler JW. 1988. Demethylated CD8 gene in CD4+ cells suggests that CD4+ cells were developed from CD8+ cell precursors. Science 242: 1174-1176   DOI
31 Kohler G and Milstein C. 1975. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 256. 495-497   DOI   ScienceOn
32 Organ BC, Antonacci AC, Chiao J, Kumar A, de Riesthal HF, et al. 1989. Changes in lymphocyte number and phenotype in seven lymphoid compartments after thermal injury. Ann Surg 210: 78-89   DOI   ScienceOn
33 Shimizu M, Yamada S, Kawashima K, et al. 1996. Changes of lymphocyte subpopulations in pigs infected with porcine reproductive and respiratory syndrome (PRRS) virus. Vet Immunol Immunopathol 50: 19-27   DOI   ScienceOn
34 Christianson WT, Choi CS, Collins JE, et al. 1993. Pathogenesis of porcine reproductive and respiratory syndrome virus infection in mid-gestation sows and fetuses. Can J Vet Res 57: 262-268
35 문진산, 박용호, 정석찬 등. 1996. 돼지 오제스키 바이러스 재조합단백질 gp50, gIII와 a-ADV을 이용한 Quill A-ISCOMs 접종시 백혈구아군 분포율에 관한 연구. 대한수의학회지 36(2) 359-369
36 Binns RM. 1978. Sheep erythrocyte rosettes in pigs, sheep, cattle and goat demonstrated in the presence of dextran. J Immunol Methods 21: 197-210   DOI   ScienceOn
37 Hommez J, Devrise LA, Castryck F. et al. 1990. Slide precipitation, a simple method to type Actinobacillus pleuropneumoniae. Vet Microbiol 24: 123-126   DOI   ScienceOn
38 황의경, 김재훈, 윤순식. 1994. 돼지 콜레라 바이러스 접종이 돼지 혈액의 림프구 아군별 분포율에 미치는 영향. 농업과학논문집 36: 576-586