• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2026-2031
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• 2007

Hydrogen is receiving much research attention as an alternative substitute for hydrocarbon fuels these days due to its cleanliness and renewability. However, hydrogen should be used with caution because of its high propensity for leak and wide flammable range. This study deals with a situation that hydrogen leaks and then forms a flammable mixture inside 1kW class residential fuel cell. The residential fuel cell was modeled as a box-shaped chamber with vent openings at the top and bottom, filled with various components such as reformer, desulfurizer, fuel cell stack and humidifier. Computational fluid dynamics (CFD) was used to simulate the diffusion, buoyant flow and accumulation of leaked hydrogen in the modeled chamber. From the simulation, the risk region vulnerable to flame was identified and the methods to minimize such hazardous region was discussed. When the vent openings are 1% of the total surface, as the quantity of hydrogen leakage increases the risk regions increases accordingly. As the vent openings of the total surface increased from 1% to 2.3%, averaged hydrogen mole fraction is under 1% in the system.

• The Korean Journal of Physiology and Pharmacology
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• 제24권1호
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• pp.47-52
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• 2020

We previously demonstrated that Bordetella bronchiseptica (B. bronchiseptica) antigen (Ag) enhances the Mycoplasma hyopneumoniae Ag-specific immune response. The focus of this study was whether acellular bacterin of B. bronchiseptica could be used as an adjuvant to increase antigen-presenting capability of dendritic cells (DCs) by increasing the level of activation. The metabolic activity of DCs was increased by B. bronchiseptica, similar to lipopolysaccharide (LPS). Flow cytometry analysis revealed that B. bronchiseptica increases the expression of major histocompatibility complex class-2, cluster of differentiation (CD)40, CD54, and CD86 which are closely related to DC-mediated immune responses. B. bronchiseptica enhanced the production of cytokines related to adaptive immune responses. Furthermore, the survival rate of B. bronchiseptica-injected groups was 100% at 15 and 20 mg/kg doses, whereas that of LPS-injected groups was only 20%, 0% at 15 and 20 mg/kg doses respectively, and so B. bronchiseptica is likely to be safer than LPS. Taken together, these results indicate that B. bronchiseptica can be used as an adjuvant to enhance the antigen-presenting capability of DCs. B. bronchiseptica is a candidate for producing vaccines, especially in case of DC-mediating efficacy and safety demands. This study provides researchers and clinicians with valuable information regarding the usage of B. bronchiseptica as a safe bacteria-derived immunostimulating agent for developing efficient vaccines.

• 약학회지
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• 제42권3호
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• pp.302-306
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• 1998

In the previous study we described the antitumor activity of GLB, a protein-polysaccharide fraction of the growing tips of Ganoderma lucidum, against sarcoma 180 solid tu mor in ICR mice. In this study we investigated the stimulatory activity of GLB on macrophages. When analyzed using a flow cytometer, GLB ($100{\mu}g/ml$) was found to increase the phagocytic activity of the BALB/c mouse peritoneal macrophages as well as chicken macrophage BM2CL cells against FITC-labeled C.albicans by 55.2% and 21.2%, respectively. GLB also increased the spreading and the expression of MHC class II molecules of BM2CL cells as well as the mouse peritoneal macrophages. From these results, it is clear that GLB is a strong stimulator to the macrophages.

• 한국해양공학회지
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• 제11권2호
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• pp.100-106
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• 1997

Viscous flow around an actual ship is calculated by an use of RANS(Reynolds-averaged Navier-Stokes) solver. Reynolds stress is modelled by using k-$\varepsilon$ turbulence model and the law of wall is applied near the body. Body fitted coordinates are introduced for the treatment of the complex boundary of the ship hull form. The transformed equations in the computational domain are numerically solved by an employment of FVM(Finite Volume Method). SIMPLE(Semi-Implcit Pressure Linked Equation) method is adopted in the calculation of pressure and the solution of the disssssssscretized equation is obtained by the line-by-line method with the use of TDMA(Tri-Diagonal Matrix Algorithme). The subject ship model of actual calculation is 4,410 TEU class container carrier. For 4 geosim models the calculated viscous resistancce values are compared with the model test results and analyzed on their componentss. The resistance performance of an actual ship is predicted very resonably, so this mothod may be utilized as a design tool of hull form.

• 에너지공학
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• 제11권3호
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• pp.254-261
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• 2002

The fabrication method for the main components of the polymer electrolyte membrane fuel cell stack such as electrodes, membrane-electrode assemblies, and bipolar plates was established for the effective electrode area of 240 ㎠. A counter-flow type 100-cell stack was fabricated by using the above components and then a maximum power of 7.44 kW for H$_2$/O$_2$ and 5.56 kW for H$_2$/air could be obtained at 70$\^{C}$ and 1 atm. It was seen that the distribution of the OCV for unit cells in the stack was uniform but the voltage deviation increased as the load increased due to the IR drop and the electrode polarization. The stack was applied to the power source of the fuel cell/battery hybrid electric golf car. It produced about 1 kW at a room temperature operation during the test run, which occupied about 43% of the total power required by the 2.3 kW motor.

• 대한수학교육학회지:학교수학
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• 제17권1호
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• pp.65-78
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• 2015

본 연구는 그래프 해석에 대한 유사한 오류를 범하는 초등학교 5학년 수학 영재 학생 4명을 대상으로 CBR을 활용한 그래프 활동이 그래프 능력에 미치는 효과를 분석하였다. 선행연구에 기초하여 CBR을 활용한 그래프 활동 수업 설계를 하였으며 40분 동안 2차시에 걸쳐 영재 교사에 의해 수업이 진행되었다. 자료 분석을 위해 동영상 촬영, 학생과의 인터뷰 등을 수집하여 녹취록을 작성하였고, 2주 후 동일한 문항으로 사후 검사를 실시하여 연구 참여자의 그래프 해석에 대한 오류가 어떤 변화를 거치는지 비교 분석하였다. 본 연구 결과에 따르면 학생들은 그래프의 변화를 이해하고, 변인 간의 관계를 진술하며, 종속 변수 간 관련짓기를 할 수 있었다.

• 대한수의학회지
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• 제36권2호
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• pp.337-348
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• 1996

The proportion of leukocyte subpopulation in the host is of a great importance in understanding their functions and disease progress. Many methods have been developed to seperate leukocytes and to measure their activities. Characterization of immune cell subpopulations in Korean native cattle was performed using a set of monoclonal antibodies specific which are specific to bovine leukocyte differentiation antigen. Peripheral blood leukocytes from fifty Korean native and ten Holstein cattle were collected and analyzed for the investigation of leukocyte subpopulation by using monoclonal antibodies and flow cytometry. The result indicated that Korean native cattle have significantly higher proportion of leukocyte subpopulations expressing MHC class II molecules and BoCD4 than Holstein cattle.

• Journal of Microbiology and Biotechnology
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• 제22권8호
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• pp.1066-1076
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• 2012

Previous observations demonstrated that various immunosuppressive agents and their combination therapies can increase allograft survival rates. However, these treatments may have serious side effects and cannot substantially improve or prolong graft survival in acute graft-versus-host disease (GVHD). To improve the therapeutic potency of divalent immunoadhesins, we have constructed and produced several tetravalent forms of immunoadhesins comprising each of cytotoxic T-lymphocyte-associated antigen-4 (CTLA4), CD2, and lymphocyte activation gene-3 (LAG3). Flow cytometric and T cell proliferation analyses displayed that tetravalent immunoadhesins have a higher binding affinity and more potent efficacy than divalent immunoadhesins. Although all tetravalent immunoadhesins possess better efficacies, tetravalent forms of CTLA4-Ig and LAG3-Ig revealed higher inhibitory effects on T cell proliferation than tetravalent forms of TNFR2-Ig and CD2-Ig. In vitro mixed lymphocytes reaction (MLR) showed that combined treatment with tetravalent CTLA4-Ig and tetravalent LAG3-Ig was highly effective for inhibiting T cell proliferation in both human and murine allogeneic stimulation. In addition, both single tetravalent-form and combination treatments can prevent the lethality of murine acute GVHD. The results of this study demonstrated that co-blockade of the major histocompatibility complex class (MHC)II:T cell receptor (TCR) and CD28:B7 pathways by using tetravalent human LAG3-Ig and CTLA4-Ig synergistically prevented murine acute GVHD.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2008년도 한국컴퓨터종합학술대회논문집 Vol.35 No.1 (A)
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• pp.174-178
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• 2008

P2P를 포함하는 인터넷 애플리케이션 트래픽의 보다 빠르고 정확한 분류는 최근 학계의 중요한 이슈 중 하나이다. 본 논문에서는 기존의 전통적인 분류방법으로 대표되는 port 번호 및 payload 정보를 이용하는 방법론의 구조적 한계점을 극복하는 새로운 대안으로써, 이진 분류기인 SVM과 단일클래스 SVM을 계층적으로 결합한 다중 클래스 SVM을 구축하여 인터넷 애플리케이션 트래픽 분류를 수행하였다. 제안된 시스템은 이진 분류기인 SVM으로 P2P 트래픽과 non-P2P 트래픽을 빠르게 분류하는 첫 번째 계층, 3개의 단일클래스 SVM을 기반으로 P2P 트래픽들을 파일공유, 메신저, TV로 분류하는 두 번째 계층, 그리고 전체 16가지의 애플리케이션 트래픽별로 세분화 분류하는 세 번째 계층으로 구성된다. 제안된 시스템은 flow 기반의 트래픽 정보를 수집하여 인터넷 애플리케이션 트래픽을 coarse 혹은 fine하게 분류함으로써 효율적인 시스템의 자원 관리, 안정적인 네트워크 환경의 지원, 원활한 bandwidth의 사용, 그리고 적절한 QoS를 보장하였다. 또한, 새로운 애플리케이션 트래픽이 추가되더라도 전체 시스템을 재학습 시킬 필요 없이 새로운 애플리케이션 트래픽만을 추가 학습함으로써 시스템의 점증적 갱신 및 확장성에도 기여하였다. 평가항목인 recall과 precision에서 만족스러운 수치 등을 실험을 통하여 확인함으로써 제안된 시스템의 성능을 검증하였다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2011년도 제37회 추계학술대회논문집
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• pp.135-138
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• 2011

한국형 달착륙선 개발을 위한 기초연구로 달착륙선 지상시험용 추진시스템의 개발을 진행 중이다. 착륙선의 하강을 위한 추력기는 200 N 급으로 설계유량 100 g/s, 연소실 압력 200 psi, 진공추력 220 N을 목표로 설계/제작 하였다. 연소시험을 위해 LM guide(Linear Motion Guide)를 이용한 추력시험장치를 꾸며 연소시험을 수행하였으며, 그 결과 연소실 압력 210 psi 일 때 유량은 96.1 g/s가 흘렀으며 그에 따른 추력은 약 160 N으로 측정되었다.