• IMMUNE NETWORK
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• 제22권5호
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• pp.42.1-42.20
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• 2022

Vaccination with tumor peptide epitopes associated with MHC class I molecules is an attractive approach directed at inducing tumor-specific CTLs. However, challenges remain in improving the therapeutic efficacy of peptide epitope vaccines, including the low immunogenicity of peptide epitopes and insufficient stimulation of innate immune components in vivo. To overcome this, we aimed to develop and test an innovative strategy that elicits potent CTL responses against tumor epitopes. The essential feature of this strategy is vaccination using tumor epitope-loaded nanoparticles (NPs) in combination with polyinosinic-polycytidylic acid (poly-IC) and anti-PD1 mAb. Carboxylated NPs were prepared using poly(lactic-co-glycolic acid) and poly(ethylene/maleic anhydride), covalently conjugated with anti-H-2K^b mAbs, and then attached to H-2K^b molecules isolated from the tumor mass (H-2^b). Native peptides associated with the H-2K^b molecules of H-2K^b-attached NPs were exchanged with tumor peptide epitopes. Tumor peptide epitope-loaded NPs efficiently induced tumor-specific CTLs when used to immunize tumor-bearing mice as well as normal mice. This activity of the NPs significantly was increased when co-administered with poly-IC. Accordingly, the NPs exerted significant anti-tumor effects in mice implanted with EG7-OVA thymoma or B16-F10 melanoma, and the anti-tumor activity of the NPs was significantly increased when applied in combination with poly-IC. The most potent anti-tumor activity was observed when the NPs were co-administered with both poly-IC and anti-PD1 mAb. Immunization with tumor epitope-loaded NPs in combination with poly-IC and anti-PD1 mAb in tumor-bearing mice can be a powerful means to induce tumor-specific CTLs with therapeutic anti-tumor activity.

• 문화기술의 융합
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• 제10권1호
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• pp.227-233
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• 2024

한국은 '22년 역대 최대의 방산수출 실적을 달성하였다. 방산수출은 한국의 무기를 외국에 수출하는 것으로 방위산업 진흥을 위한 가장 효과적인 방법이다. 또한, 우수한 무기체계를 지속적으로 공급하려면 견실한 방위산업이 구축되어 있어야 하기 때문에 방산수출은 국가차원의 중요한 사안으로 정부는 대통령실을 콘트롤타워로 적극적인 지원정책을 펼치고 있다. 특히 한국의 방산수출 빅4 진입이라는 화두가 제기되고 있는데, 본 논문은 기존에 제시된 방산수출 진흥 방안과는 달리 혁신적인 방안으로서 세계 수준의 무기체계를 보유할 수 있는 첨단기술 확보와 구매국의 요구에 맞춤형으로 수출할 수 있는 공동연구개발이라는 전략적인 방안을 제시하고 구체적으로 실행할 수 있는 절차를 제시하였다. 즉 첨단기술 확보를 위해 군은 기존의 방식을 탈피하여 과감한 첨단기술 선정과 충분한 예산 지원을 하고 개발기관에게 자율성을 부여해야 하며 이 기술이 첨단무기에 적기에 적용되도록 신속개발시스템도 도입해야 한다. 수출 공동연구개발은 구매국의 첨단기술 보유 의지를 고려하여 초민감 핵심기술을 제외한 유연한 기술이전을 추진하고 전략적 파트너십을 형성하는 방향으로 나아가도록 하는 것이다. 이를 통해 한국의 방산수출 변화의 시점에서 획기적이고 새로운 전략을 통하여 한국의 방산수출이 세계 빅4에 진입하는데 도움이 되도록 하였다.

• 벤처창업연구
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• 제9권4호
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• pp.27-39
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• 2014

기술사업화를 위한 연구개발 센터 조직은 크게 세 가지 단계로 구분된다. 첫째는 산업계의 문제를 해결하는 역량을 개발하는 것이며, 둘째는 선진국을 따라잡기 위해 수입된 기술을 창조적 모방을 통해 개발하는 것이다. 셋째 단계는 선진화된 지식창조 역량을 습득하는 것이다. 따라서 센터의 조직은 대학과 정부의 발전전략과 잘 연계되어야 한다. 본 연구는 개발도상국에게 연구센터의 개발을 통해 경제개발과 관련된 관리적 시사점을 제공하고 있다. 사례연구로 든 카자흐스탄의 경우 산업계 문제해결 단계인 첫 번째 단계에서 센터는 기술과 인적자원 측면에서 인프라구조를 구축한다. 두 번째 단계는 지식 역량을 구축하는 것으로 중앙 아시아에서 주요 산업 연구개발 허브로서의 역할을 하는 것이다. 셋째 단계에서는 센터가 선진화된 지식을 구축하여 세계수준의 지식센터를 만드는 것이다. 이같은 점에서 연구개발과 사업화 센터의 진화는 센터의 서비스 형태에 따라 다르게 나타나야 할 것이다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.33-33
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• 2015

Clubroot disease is the major threat to the production of Chinese cabbage (Brassica rapa L.) in Japan. Although the breeding of the clubtoot resistant (CR) cultivars is one of the most efficient ways to control this disease, the CR cultivars do not always have effects due to the breakdown of resistance. Therefore, it is necessary to develop the breeding strategy to accumulate multiple CR genes in a single cultivar effectively. We have identified two incomplete dominant CR loci, Crr1 and Crr2, which are originated from the European CR turnip Siloga. To investigate the effectiveness of marker-assisted selection (MAS) for CR breeding, the inbred line with Crr1 and Crr2 was crossed with parental lines of the existing CR $F_1$ cultivar of Chinese cabbage, followed by 5 times of MAS and backcrossing. The $F_1$ derived from a cross between the resulting parental lines improved the clubroot resistance as expected and had the same morphological characters as the original $F_1$ cultivar. We have shown that the Crr1 locus comprised two loci: Crr1a, which by itself conferred resistance to the mild isolate; and Crr1b, which had a minor effect, but was not required for Crr1a-mediated resistance. Further genetic analysis suggested that Crr1b was necessary to acquire resistance to the more virulent isolate in combination with Crr2. Molecular characterization of Crr1a encoding TIR-NB-LRR class of R protein revealed that there were at least 4 alleles in Japanese CR cultivars of Chinese cabbage. PCR analysis with Crr1a-specific markers demonstrated that the functional alleles were predicted to be present in European CR turnips, Debra and 77b besides Siloga, whereas rarely in Japanese CR cultivars, indicating that Crr1a is an useful source to improve the resistance of Chinese cabbage cultivars.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2503-2508
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• 2013

Histone deacetylation mediated by histone deacetylases (HDACs) has been reported as one of the epigenetic mechanisms associated with tumorigenesis. The poor responsiveness of anticancer drugs found with cholangiocarcinoma (CCA) leads to short survival rate. We aimed to investigate mRNA expression of HDACs class I and II, and the effect of HDAC inhibitors, suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA), in CCA in vitro. Expression of HDACs was studied in CCA cell lines (M213, M214 and KKU-100) and an immortal cholangiocyte (MMNK1) by semi-quantitative reverse transcription-PCR. SAHA and VPA, as well as a classical chemotherapeutic drug 5 -fluorouacil (5-FU) were used in this study. Cell proliferation was determined by sulforhodamine assay. $IC_{50}$ and $IC_{20}$ were then analyzed for each agent and cell line. Moreover, synergistic potentional of VPA or SAHA in combination with 5-FU at sub toxic does ($IC_{20}$) of each agent was also evaluated. Statistic difference of HDACs expression or cell proliferation in each experimental condition was analyzed by Student's t-test. The result demonstrated that HDACs were expressed in all studied cell types. Both SAHA and VPA inhibited cell proliferation in a dose-dependent manner. Interestingly, KKU-100 which was less senstitive to classical chemotheraoeutic 5-FU was highly was sensitive to HDAC inhibitors. Simultaneous combination of subtoxic doses of HDAC inhibitors and 5-FU signiicantly inhibited cell proliferation in CCA cell lines compared to single sgent treatment($P{\leq}0.01$), while sequentially combined treatments were less effective. The present study showed inhibitory effects of HDACIs on cell proliferation in CCA cell lines, with synergistic antitumor potential demonstrated by simultaneous combination of VPA or SAHA with 5-FU, suggesting a novel alternative therapeutic strategy in effective treatment of CCA.

• 한국환경복원기술학회지
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• 제4권3호
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• pp.30-37
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• 2001

This study was conducted to investigate physicochemical property changes from July 1998 to August 2001 in Mt. Bukhansan National Park. Four water sampling points were selected to measure the quality of stream water in the northeastern part of the Mt. Bukhansan National Park. The results were summarized as follows; In spring, the average pH of stream water was below the first class of the river water quality standard, while it was normal level in summer. The average electrical conductivity was about 2.3~3.3 times higher in downstream water than in upstream water during spring and summer. The contents of anions($Cl^-$, $NO{_3}^-$, $SO{_4}^{2-}$) were about 1.1~7.4 and 0.4~11.4 times higher in downstream than in upstream water, respectively. These results indicate that water quality was poorer in downstream than in upstream water. We suggest that stream water in the Mt. Bukhansan National Park should be protected from impacts of snow melting mineral particles in spring season and human impacts like wastewater of point source in summer season.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.783-788
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• 2003

In this paper, we present a new approach to detect and recognize human face in the image from vision camera equipped on the mobile robot platform. Due to the mobility of camera platform, obtained facial image is small and pose-various. For this condition, new algorithm should cope with these constraints and can detect and recognize face in nearly real time. In detection step, ‘coarse to fine’ detection strategy is used. Firstly, region boundary including face is roughly located by dual ellipse templates of facial color and on this region, the locations of three main facial features- two eyes and mouth-are estimated. For this, simplified facial feature maps using characteristic chrominance are made out and candidate pixels are segmented as eye or mouth pixels group. These candidate facial features are verified whether the length and orientation of feature pairs are suitable for face geometry. In recognition step, pseudo-convex hull area of gray face image is defined which area includes feature triangle connecting two eyes and mouth. And random lattice line set are composed and laid on this convex hull area, and then 2D appearance of this area is represented. From these procedures, facial information of detected face is obtained and face DB images are similarly processed for each person class. Based on facial information of these areas, distance measure of match of lattice lines is calculated and face image is recognized using this measure as a classifier. This proposed detection and recognition algorithms overcome the constraints of previous approach [15], make real-time face detection and recognition possible, and guarantee the correct recognition irregardless of some pose variation of face. The usefulness at mobile robot application is demonstrated.

• 농촌지도와개발
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• 제20권4호
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• pp.989-1021
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• 2013

농어업유산의 지역 활성화 전략으로서 에코뮤지엄을 통한 유산관광의 활성화 방안을 살펴봤다. 에코뮤지엄은 '살아 있는 유산의 역동적 보전'이라는 농어업유산제도의 목적을 실현할 수 있는 중요한 수단이 될 수 있다. 하지만 실제 사례를 적용해본 결과, 유산으로서 가치가 높고 유산관광을 추진하고 있었으나, 유산관광의 4요소(진정성, 교육성, 엔터테인먼트, 향수)를 대비해보면 유산관광의 체계가 갖춰져 있지 않았다. 또한, 에코뮤지엄으로서 가능성이 높았으나, 에코뮤지엄의 4요소(유산, 박물관, 참여, 콘텐츠)별로 살펴보면 체계가 미흡하고 해결해야할 과제가 많았다. 에코뮤지엄을 통한 지역 활성화 성과를 거두기 위해서는 에코뮤지엄의 4요소를 구축하고, 유산관광의 4요소가 충족될 수 있도록 진정성 있는 콘텐츠 개발, 주민 창조계급 이해관계자의 참여, 사이트 구축, 다양한 학술적 접근, 주민소득 연계방안 마련 등이 필요하다. 또한, 국가농어업유산을 에코뮤지엄으로 조성할 경우에 박물관으로서 정부지원이 가능하도록 법적 근거를 마련해야 한다.

• 한국콘텐츠학회논문지
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• 제9권12호
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• pp.253-260
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• 2009

세계대전 이후 유럽경제는 경제회복과 국가이익을 위해 산업과 기업을 국영화하면서 파생된 문제점의 대안으로, 황금주가 영국을 비롯하여 유럽의 많은 나라에 널리 퍼지게 되었다. 그러나 영국 등 유럽 여러 나라에서 채택한 황금주가 유럽재판소에서 "자본이동의 자유"를 침해한다는 이유로 부정적인 판결을 받았다. 그러나 최근 프랑스와 독일에서 중동이나 아시아국가의 풍부한 자금으로부터 국가의 전략적 회사의 적대적 매수로부터 보호하기 위하여 황금주를 적극적으로 고려하고 있다. 이러한 세계경제의 흐름에서 보면, 우리나라 역시 현실을 직시하면 원시정관이나 대다수 주주의 동의 등 기존주주의 보호를 전제로 매우 엄격한 요건을 갖추어 도입하도록 하여야 할 것이다.

• IMMUNE NETWORK
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• 제13권5호
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• pp.194-198
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• 2013

Recent papers have shown that the initial event in the pathogenesis of autoimmune type 1 diabetes (T1D) comprises sensing of molecular patterns released from apoptotic ${\beta}$-cells by innate immune receptors such as toll-like receptor (TLR). We have reported that apoptotic ${\beta}$-cells undergoing secondary necrosis called 'late apoptotic' ${\beta}$-cells stimulate dendritic cells (DCs) and induce diabetogenic T cell priming through TLR2. The role of other innate immune receptors such as TLR7 or TLR9 in the initiation of T1D has also been suggested. We hypothesized that TLR2 blockade could inhibit T1D at the initial step of T1D. Indeed, when a TLR2 agonist, $Pam3CSK_4$ was administered chronically, the development of T1D in nonobese diabetic (NOD) mice was inhibited. Diabetogenic T cell priming by DCs was attenuated by chronic treatment with $Pam3CSK_4$, indicating DC tolerance. For the treatment of established T1D, immune tolerance alone is not enough because ${\beta}$-cell mass is critically reduced. We employed TLR2 tolerance in conjunction with islet transplantation, which led to reversal of newly established T1D. Dipeptidyl peptidase 4 (DPP4) inhibitors are a new class of anti-diabetic agents that have beneficial effects on ${\beta}$-cells. We investigated whether a combination of DPP4 inhibition and TLR2 tolerization could reverse newly established T1D without islet transplantation. We could achieve normoglycemia by TLR2 tolerization in combination with DPP4 inhibition but not by TLR2 tolerization or DPP4 inhibition alone. ${\beta}$-cell mass was significantly increased by combined treatment with TLR2 tolerization and DPP4 inhibition. These results suggest the possibility that a novel strategy of TLR tolerization will be available for the inhibition or treatment of established T1D when combined with measures increasing critically reduced ${\beta}$-cell mass of T1D patients such as DPP4 inhibition or stem cell technology.