• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.4
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• pp.460-466
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• 2016

From 2013 through 2015, we investigated the contamination status and antimicrobial resistance patterns of pathogenic Vibrio parahaemolyticus in commercially valuable seawater and shellfish (Oyster Crassostrea gigas, short-neck clam Venerupis philippinarum, ark shell Scapharca broughtonii and mussel Mytilus galloprovinciallis) from the southern coast of Korea. The detection rate of V. parahaemolyticus was highest in short-neck clams (23.7%), followed by ark shells (19.2%), oysters (15.9%), mussels (13.6%), and seawater (8.6%). The following percentages of PCR assays of shellfish were positive for the thermostable direct hemolysin-related hemolysin gene (trh) : oysters (12.8%), short-neck clams(11.8%), and ark shells (3.4%). Similar assays for the thermostable direct hemolysin gene (tdh) resulted in positive results for short-neck clams (5.9%) and ark shells (3.4%). Antimicrobial resistance was present in 100% of 8 tdh (+) and 2 trh (+) V. parahaemolyticus isolates challenged with ampicillin. However, all pathogenic V. parahaemolyticus were sensitive to 14 other antibiotics. To ensure the safety of shellfish consumption, the continuous monitoring of the prevalence and distribution of virulence factors of V. parahaemolyticus in shellfish farms is needed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.2
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• pp.121-126
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• 2018

The purpose of this study was to assess bacteriological hazards in the following 7 kinds of minimally processed shellfish products purchased from Korean seafood retail outlets: raw oysters stored with seawater in polyethylene bags, frozen oysters, raw mussels, frozen mussels, boiled mussels, raw short-necked clam, and frozen short-necked clams, obtained from Korean seafood retail outlets. The total coliform and Escherichia coli counts determined in all samples were detected below regulation limit of the Korean government guidelines (Food Code). In addition, the high-risk bacterial pathogens, Listeria monocytogenes, Salmonella spp., and E. coli O157:H7 were not detected in any samples. Low-risk pathogens such as Staphylococcus aureus and Vibrio parahaemolyticus were detected at levels above $1.0{\times}10^2$ colony forming unit (CFU)/g in some minimally processed shellfish products. Notably, S. aureus was detected in all samples. Raw oysters stored with seawater in polyethylene bags, frozen oysters, and boiled blue mussels are commonly ingested without heating, and therefore these minimally processed shellfish products pose bacteriological hazards. The detection of S. aureus in all shellfish products, an organism indicative of poor personal hygiene, which can grow and multiply during distribution, indicates the necessity of improving the food safety of minimally processed shellfish products.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.5
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• pp.752-760
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• 2020

Acute hepatopancreatic necrosis disease (AHPND), previously known as early mortality syndrome (EMS), is an emerging disease in shrimp caused by Vibrio parahaemolyticus. Some V. parahaemolyticus strains are associated with foodborne diseases in humans. To date, studies on the relationship between AHPND and pathogenic V. parahaemolyticus are very limited. In this study, we monitored the thermostable direct hemolysin-related hemolysin (trh) gene and AHPND-related genes, such as Photorhabdus insect-related (pir) genes, in 892 strains of V. parahaemolyticus isolated and identified in 24 areas of the West Coast of Korea from May to October 2019. The trh gene was detected in 9.6% of the isolates from short neck clam samples. However, the pirA and pirB genes related to AHPND were not found in any of the isolates despite using both duplex and nested PCR assays, suggesting that AHPND-related genes were nonexistent in the V. parahaemolyticus strains isolated. This study contributes to the current understanding of the relationship between AHPND and V. parahaemolyticus in Korea, as well as provides data on spatial and seasonal distributions of V. parahaemolyticus.

• Journal of Korea Multimedia Society
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• v.7 no.7
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• pp.956-966
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• 2004

The Korean animation has enjoyed the brisk formation and establishment of its world-class infra for the last several years without unified titles or concepts, under the name of a national strategic project in the age of digital image. It also enjoys its new evaluation as digital animation that it's one of the greatest money making business in the non-education and frivolous culture and has the closest relations with the modern time. A great portion of popular image media has been taken by animation for the last 30 years. In this paper we propose a motion algorithm using an animation technology. It was developed for education purposed and accessible through the internet. For instance, in Cyber Clam Museum, 1000 gesture contents, the visual processes were used to design a screen with a realistic image and create an animation that makes possible show at 360 and every such transformation as translation, rotation, and scaling can be applied in the image interactively for the convenient and effective viewing.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.4
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• pp.352-358
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• 2017

The object of this study is to assess physicochemical and microbiological hazards involved during the processing of shellfish products such as oysters, clam, and mussels. Samples including raw materials, intermediates, and final products in the processing process were collected from seven simple-processed shellfish facilities. In the samples obtained from the facilities, viable cell count and coliform group were detected less than that of the Korean Government guidelines. In addition, the high-risk bacterial pathogens such as Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes were not identified in raw materials and final products. However, the low-risk pathogens, Staphylococcus aureus and Vibrio parahaemolyticus, were detected in some final products. The level of heavy metal content in the final products tested in this study meets the recommendations by the Korean Government standard guidelines. No foreign materials in the final products were also observed. Considering these results, it was concluded that no significant food hazards exists in the processing process of simple-processed shellfish products. However, it is essential to improve the food safety control in the shellfish processing facilities since S. aureus, a personal sanitary indicative bacterium, was continuously detected over seven simple-processed shellfish facilities.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.4
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• pp.413-420
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• 2017

The increase in the number of mud shrimps Upogebia major is a concern because of their negative effects on shellfish aquaculture, including Manila clam Venerupis philippinatum along the west coast of Korea. This study developed a behavioral analysis system for aquatic animals using a set of monochromatic light-emitting diode (LED) modules covering the visible light range at similar intervals. Movements of mud shrimp were monitored using a tracking system under illumination with infra-red light and an LED of 660 nm wavelength without provoking stimulation. The minimum light intensity needed to induce a photoresponse by the mud shrimp was $10{\mu}mole/m^2/s$ under the conditions tested. Of the six kinds of LED illuminations tested, the most sensitive response was obtained with illumination with the 505 nm LED, followed in order by LEDs with peak wavelengths of $525nm{\fallingdotseq}465nm$ > $405nm{\fallingdotseq}590nm$ > 660 nm. These findings should help to identify LED sources that efficiently induce movement of the mud shrimp and also for monitoring movement without stimulating.

• Parasites, Hosts and Diseases
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• v.31 no.1
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• pp.7-12
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• 1993

Metaceriae of Parvatrema timondavidi (Digenea; Grmnophallidae) were found from Tapes phillippinarum, one of the most common marine clams in Korean. T. were philippinarum was collected from a fishery market in seoul, and all of the clams eximined were found to contain many gymnophyallid metacercariae. To get adult worms, 10 ICR mice were fed with 100 metacercariae each and sacrificed by cervical dislocation at 7, 10 and 14 mice were morphologically characterized by oval body shape, lage oral sucker with lateral located genital pore from the venttral sucker. Based on these characters they identified as P.timondawvidi Bartoli, 1964. this study first confirms the presence of P. timondawcidi metacercartae in T.philippinarum in Korea.

• Korean Journal of Food Science and Technology
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• v.20 no.3
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• pp.441-446
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• 1988

Alkaline treatment during preparation of anchovy extracts was studied for its changes in some of the physicochemical and sensory qualities. The dried anchovy was blended in 0-0.5N NaOH solutions and then incubated at $60^{\circ}C$ for 6 hours. After extraction the suspensions were neutralized and centrifuged. The results showed that the yields of solids and protein were increased by 3-5 fold of those of water extract as alkaline concentration and treatment time increased. The intrinsic viscosity showed little changes for the extracts prepared with 0-0.2N NaOH solution while the extracts prepared with over 0.3N NaOH resulted a initial small decrease followed by a rapid increase to the maximum point. The changes in color expressed as Hunter 'L', 'a' and 'b' values showed that the L value increased rapidly until 3 hours of treatment followed by a decrease, and 'a' and 'b' values were increased a little. The intensities of odor and taste were markedly increased by 2-3 fold for all of the descriptions investigated where clam-like odor and taste and sea complex odor were particularly significant.

• Journal of Wetlands Research
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• v.12 no.3
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• pp.107-127
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• 2010

This research is about the monitoring of people's consciousness on ecosystem change after coastal restoration project in Gangjin Bay. Tidal flat ecosystem of Ganjin Bay was major producing district of short-necked clam in Jeonnam Province. Owing to coastal restoration project (sand gathering and dredging) during 5 years, however, ecosystem was disturbed and harvesting of fish was decreased. Although a few of ark shell and oyster are harvested but drastically decreased. Even aging and depopulation is general situation like other rural regions, life pattern of villages and depopulation in Gangjin Bay was influenced by drastic decreasing of income. As the results of social monitoring, drastic decreasing of fish and shell in Gangjin Bay is significantly concerned with coastal restoration project (sand gathering and dredging) and also with difficulty of fresh-water inflow to coastal bay because of Jangheung Dam. In order to get the detail information on the cause and consequence of ecosystem change in tidal flat, it is necessary to apply the long-term socio-economic monitoring as well as biological and ecosystem monitoring.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.11
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• pp.5642-5670
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• 2017

Antivirus is an important issue to the security of virtual machine (VM). According to where the antivirus system resides, the existing approaches can be categorized into three classes: internal approach, external approach and hybrid approach. However, for the internal approach, it is susceptible to attacks and may cause antivirus storm and rollback vulnerability problems. On the other hand, for the external approach, the antivirus systems built upon virtual machine introspection (VMI) technology cannot find and prohibit viruses promptly. Although the hybrid approach performs virus scanning out of the virtual machine, it is still vulnerable to attacks since it completely depends on the agent and hooks to deliver events in the guest operating system. To solve the aforementioned problems, based on in-memory signature scanning, we propose an agentless runtime antivirus system VirtAV, which scans each piece of binary codes to execute in guest VMs on the VMM side to detect and prevent viruses. As an external approach, VirtAV does not rely on any hooks or agents in the guest OS, and exposes no attack surface to the outside world, so it guarantees the security of itself to the greatest extent. In addition, it solves the antivirus storm problem and the rollback vulnerability problem in virtualization environment. We implemented a prototype based on Qemu/KVM hypervisor and ClamAV antivirus engine. Experimental results demonstrate that VirtAV is able to detect both user-level and kernel-level virus programs inside Windows and Linux guest, no matter whether they are packed or not. From the performance aspect, the overhead of VirtAV on guest performance is acceptable. Especially, VirtAV has little impact on the performance of common desktop applications, such as video playing, web browsing and Microsoft Office series.