• The Korean Journal of Mycology
• /
• v.14 no.1
• /
• pp.1-8
• /
• 1986

The following ten species collected from the air of Seoul City and the soils in Korea are reported as undescribed to Korean fungal flora: Cladosporium sphaerospermum, C. herbarum, C. colocasiae, Alternaria chlamydospora, A. cheiranthi, A. citri, Curvularia ovoidea, C. inaequalis, C. affinis, and Eurotium repens.

• The Plant Pathology Journal
• /
• v.17 no.6
• /
• pp.350-353
• /
• 2001

In 2001, a black scab disease was observed in tea plant (Thea sinensis) cultivated in the hillsides of Hwngaemyon and Hadong-gun, Gyeongnam province, Korea. The disease symptoms initially appeared on leaves, green twigs and stems, showing small dark brown spots on the infected areas, which gradually expanded. A fungus was isolated from diseased leaves and green twigs. It grew readily on potato dextrose agar, forming dark green to dark gray colonies. The optimum temperature for mycelial growth was about 20$^{\circ}C$. The diameter of growing hyphae was 3.5-5.8 $\mu\textrm{m}$. Conidia were ellipsoidal, ovoid or subspherical, and mostly one-celled but occasionally septate. The size of one-celled and septate conidia were 3.7-12.4${\times}$3.4-5.2 $\mu\textrm{m}$ and 9.3-18.7${\times}$3.8-7.2 $\mu\textrm{m}$, respectively. Conidia were formed in long branched chains on the erected conidiophores, which were dark brown in color and 28.9-218.3${\times}$3.0-6.1 $\mu\textrm{m}$ in length. The fungus was identified as Cladosporium herbarum on the basis of its morphological characteristics. The black scab disease occurring in tea caused by Cladosporium herbarum has not been previously reported in Korea.

• Mycobiology
• /
• v.29 no.2
• /
• pp.110-112
• /
• 2001

A black scab was observed on strawberry(Fragaria ananassa) in plastic film houses around Jinju area during the winter of 2000. The disease started from leaves then moved to calyx and runner. At the beginning, the infected area started with small dark brown spots then gradually expanded. The pathogenic fungus was purely isolated from the diseased leaves, calyx and runner. The fungus was inoculated to test Koch's postulates and proved to be the causal agent of the disease. The isolated fungus grew readily on potato dextrose agar, forming dark green to dark gray colonies. The optimum temperature for mycelial growth was about $25^{\circ}C$. The diameter of growing hyphae was $3.8{\sim}5.6{\mu}m$. Conidia were ellipsoidal, ovoid or subspherical, mostly one-celled but occasionally septate. The size of conidia were $4.1{\sim}11.7{\times}3.8{\sim}5.3$ 1-cell, $9.3{\sim}18.8{\times}4.0{\sim}7.4{\mu}m$ 2-cell and formed in long branched chains on the erected conidiophores which were dark brown and variable in length between $28.8{\sim}236.2{\times}3.0{\sim}6.2{\mu}m$ in size. The fungus was identified as Cladosporium herbarum on the basis of its morphological characteristics. The black scab disease of strawberry caused by C. herbarum has not been reported in Korea previously.

• Korean Journal of Plant Resources
• /
• v.10 no.3
• /
• pp.231-234
• /
• 1997

Four urushiol components isolated from the sap of Korean lacquer tree(Rhus vernicifera Stokes) showed a strong antifungal activity, but they have no or low activity the bacteria and yeasts. Among them, 3-pentadecylcatechol marked the highest activity on the spore germination of Cladosporium herbarum ($MIC:4{\mu}g/ml$).

• Korean Journal of Plant Resources
• /
• v.11 no.1
• /
• pp.40-46
• /
• 1998

For the comparison of the urushiol composition and biological acitivity between the fresh sap and fire distilled sap of lacquer tree(Rhus vernicifera), we analysed the urushiol composition by HPLC and EI-MS, and investigated the antioxidative , antimicrobial and anticancer acitivities according to solvent fractionations. There was no difference in the urushiol composition between fresh and fire distilled saps of lacquer tree. The hexane frqctionsof two saps showed a strong DPPH radical scaverging activity (RC50 : 7.0-7.5$\mu\textrm{g}$). They also showed a strong antifungal activity onthe spore germination of Cladosporium herbarum(MIC : 8$\mu\textrm{g}$/ml), whereas they have no or low activity against the bacteria(BAcillus subtilis , Escherichia coli). In addition , hexane and butanol fractions of two saps showed a strong inhibitory activity against cultured tumour cell lines (GI50 : 0.35, 12.29$\mu\textrm{g}$/ml) in vitro. These results confirm that the fresh sap and fire distilled sap might have the similar urushiol compositions and biological activities.

• Korean Journal of Medicinal Crop Science
• /
• v.10 no.3
• /
• pp.222-229
• /
• 2002

Ixeris dentata root were extracted with methanol and then fractionated with n-hexane, EtOAc and BuOH to get active fractions. and their antioxidant and antimicrobial activities in each fraction were determined. Ethyl acetate fraction of Ixeris dentata root showed strong antioxidant activities, but aqueous fraction did not show any activities. But in the antimicrobial test, aqueous fraction showed strong antimicrobial activities except to Escherichia coli. especially, aqueous fraction showed the strongest activities against Hypocrea nigricans. and butanol fraction showed the strongest activities against Cladosporium herbarum. This study was performed to determine the antimutagenic and cytotoxic effect of Ixeris dentata root methanol extract on Salmonella typhimurium TA98, TA100 and cancer cell lines using ames test and cytotoxicity assay, respectively. Cancer cell lines include human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma (Hep 3B). Futher fractionations with hexane, ethyl acetate, butanol and water from methanol extract of Ixeris dentata root were performed to obtain effective fraction, methanol extracts showed 79.94% inhibitory effect on the mutagenesis induced by N' -methyl- N' -nitro-N-nitrosoguanidine(MNNG) against TA100, while 89.99% inhibition was observed on the mutagenesis induced by 4-nitroquinoline-1-oxide(4NQO) against TA98. In the meanwhile, butanol fraction showed 89.92% and 71.01% inhibitory effect on the mutagenesis induced by benzo(a)pyrene(B(a)P) against TA98 and TA100, respectively. Ethyl acetate fraction showed the strongest effect against A549, MCF-7 and Hep3B at the same concentration compared to those of other fration.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.6
• /
• pp.297-304
• /
• 2001

Scrophularia buergeriana Misrule has been contaminated with various pathogens in condition of field and storage period. This study was carried out for production of multiple stress resistance plant containing disease resistance that CGST gene expressed in transgenic Scrophularia buergeriana Misrule genome. Glutathione S-Transferases (GSTs) detoxify endobiotic and xenobiotic compounds by covalent linking of tripeptide glutathione to hydrophobic substrate. GST enzymes have been identified and characterized in insects, bacteria, and many plant species. A cDNA clone of GST was introduced into Scrophularia buergeriana Miquel by transformation with Agrobacterium tumefaciences. In coporation of the CGST gene into S. buergeriana Misrule was confirmed by PCR analysis of genomic DNA. Influence of exposure to darkness on the regeneration potential and transformation frequence were assessed. The activity of GST in transgenic plants was two times higher than that of non-transgenic plants. As a result of anti-microbe assays, the crude extract protein of transgenic plants showed the antimicrobial effects higher than control plants.

• Korean Journal of Medicinal Crop Science
• /
• v.11 no.1
• /
• pp.53-61
• /
• 2003

Cirsium japonicum var. ussuriense were extracted with methanol and then fractionated with nhexane, EtOAc and BuOH to get active fractions. And their antioxidant and antimicrobial activities in each fraction were determined. Ethyl acetate and butanol fraction of Cirsium japonicum var. ussuriense showed strong antioxidant activities, but hexane fraction did not show any activities. But in the antimicrobial test, Ethyl acetate fraction showed strong antimicrobial activities except to Aspergillus awamori, Asperigillus niger. Especially, Ethyl acetate fraction showed the strongest activities against Bacillus subtilis. And aqueous fraction showed the strongest activities against Cladosporium herbarum, Hypocrea nigricans. This study was performed to determine the antimutagenic and cytotoxic effect of Cirsium japonicum var. ussuriense methanol extract on Salmonella typhimurium TA98, TA100 and cancer cell lines using Ames test and cytotoxicity assay, respectively. Cancer cell lines include human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma (Hep3B). Futher fractionations with hexane, ethyl acetate, butanol and water from methanol extract of Cirsium japonicum var. ussuriense were performed to obtain effective fraction, methanol extract showed 60.14% inhibition effect on the mutagenesis induced by MNNG against TA100, while 77% and 72.5% inhibition was observed on the mutagenesis induced by 4NQO against TA98 and TA100, respectively. and methanol extract showed 82.25% and 73.7% inhibitory effect on the mutagenesis induced by Trp-P-1 against TA98 and TA100, respectively. methanol extract showed the strongest effect against A549, MCF-7 and Hep3B at the same concentration compared to those of other fration.