• Title/Summary/Keyword: Circular pattern

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Isolation and Identification of Microorganisms Producing the Soy Protein-Hydrolyzing Enzyme from Traditional Mejus (전통메주로부터 대두단백질 가수분해효소 생산성 미생물의 분리 및 동정)

  • Kang, Min-Jung;Kim, Seong-Ho;Joo, Hyun-Kyu;Lee, Gap-Sang;Yim, Moo-Hyun
    • Applied Biological Chemistry
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    • v.43 no.2
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    • pp.86-94
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    • 2000
  • In order to develop the enzymatic hydrolysis system concerned with taste and flavor, strains having the high hydrolyzing activity on the soy protein were selected from some traditional Mejus. Two molds and one bacterium producing enzymes which were different in character of hydrolysis were isolated and identified. Leucine and azodye enzyme activities of both M4 and M5 were relatively high among in the isolated molds. And, leucine enzyme activity of B16 was the lowest in the isolated bacteria. These strains were isolated as microorganisms having a dissimilar hydrolysis pattern on the soy protein by enzymatic reactions. Mold M4 on the culture solid media was mycelium colors of white and its sclerotia colors were changed from white to black. According to the result of slide culture, radial conidial head, subclavate vesicle, conidia of subglobose, stipes of uncolored with smooth walls and metula and phialides were existed. Because M4 was taxonomically similar to the characteristics of Aspergillus oryzae (ahlburg) species, M4 was identified and named as Aspergillus oryzae M4.Mold M5 showed white and black mycelium on the MEA medium. Mold M5 colony exhibited grayish-green color and have long(7 mm) sporangiophores at slide culture. Sporangia became brownish-gray and the wall of larger sporangia was broken to form small collars, and smaller sporangia were fomed continually from large basal membrane. Columella is globose and hyaline, and sporangiospores are ellipsoidal of small diameter$(80\;{\mu}m)$. Because M5 was taxonomically similar to the Mucor circinelloides of zygomycetes, M5 was was identified and named as Mucor circinelloides M5. Bacteria B16 colony was opaque white, circular and lobate, and had rod shaped endospore. B16 was found positive in stain, catalase, ${\beta}-glucosidse$ and V-P tests. B16 was found to utilize D-fructose, ${\alpha}-D-glucose$, maltose, D-mannose, D-raffinose, stachyose and sucrose. By the morphological and physiological results, the characteristics of B16 was thought to correspond to that of Bacillus megaterium. However, fatty acid composition was similar to Paenibacillus marcerans, requiring further study for the definite identification. Accordingly, Bacteria B16 was provisionally classified and named as Bacillus megaterium B16.

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Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model

  • Lee, Jin-Wook;Lim, Hyun-Chang;Lee, Eun-Ung;Park, Jin-Young;Lee, Jung-Seok;Lee, Dong-Woon;Jung, Ui-Won;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
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    • v.44 no.4
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    • pp.178-183
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    • 2014
  • Purpose: The aim of this study was to assess the possible paracrine effect of bone morphogeneticprotein-2 (BMP-2) at the experimental site on the adjacent control site for validating a rabbit calvarial defect model as a means of verifying the effect of BMP-2. Methods: Sixteen rabbits were divided into two groups (n=8 in each) according to whether or not BMP-2 would be used. Two circular defects (8 mm in diameter) were created side by side, 2 mm apart, in the calvarium of all of the rabbits. In each animal, one of the defects was grafted with either BMP-2-loaded carrier or carrier material alone. The control defects adjacent to these grafted defects, designated CB (the nongrafted defect adjacent BMP-2-loaded carrier-grafted defect) and CC (the nongrafted defect adjacent to carrier only-grafted defect), respectively, were the focus of this study, and were filled only with a blood clot in all of the animals. Histologic observation and histomorphometric analysis were performed at 2 and 8 weeks (n=4 animals per point in time) after surgery. Results: There was no noteworthy difference in the healing pattern, and no statistically significant differences in histomorphometric parameters such as the defect closure, new bone area, or total augmented area between the CC and CB groups. Conclusions: The results of this study suggest that rabbit calvarial defects separated by a distance of 2 mm are suitable for evaluating the effects of BMP-2 and the control defect can be regarded not to be affected by BMP-2 applied defect.

Morphological studies on the development of the prenatal and postnatal rat stomach 1. Light and scanning electron microscopical observations (랫드의 출생전·후의 위조직 발달에 관한 형태학적 연구 1. 광학현미경적 및 주사전자현미경적 관찰)

  • Chung, Heon-sik;Kim, Chong-sup;Lee, Joung-hwan
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.693-706
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    • 1997
  • The present study was designed to investigate the morphological developments of the stomach in the prenatal and postnatal rats. The gastric fundus of 16- to 22-day-old fetuses, neonates, 7-day-old, 14-day-old, 21-day-old, and adult rats were observed by light and scanning electron microscopy. Light microscopic studies. 1. In the 16-day-old fetuses, the walls of the gastric fundus were differentiated into epithelium, lamina propria, muscle layer, and serosa. The lamina propria was composed of mesenchymal connective tissue cells with sparse nucleus and their fibers. 2. In the 17-day-old fetuses, the muscular layer were differentiated into the circular and longitudinal muscle layers. 3. The epithelium on the fundus of stomach was stratified columnar cells at 16-, 17- and 18-day-old fetuses, but partly converted into simple columnar epithelium at 19-day-old fetuses. 4. The mucous cells were positive by PAS reaction at the 19-day-old fetuses and then these developed rapidly within 1 or 2 days before birth. 5. In the 20-day -old fetuses, the parietal cells were distinguished from other type cells and these cells were chiefly crowded in the middle parts of the gastric glands after 7-day-old. Scanning electron microscopic studies. 6. The surface of gastric mucosa was covered with forms of the various protrusions by forming villi at the last few days before birth and these protrusion forms were fused and folded each other. 7. The mucosal surface was closely packed by polygonal mucous cells. The openings of gastric glands were seen as slit-like round invagination and were more numerous and increased gradually in depth after the postnatal life. The above findings indicate that prominent changes occurred in the pattern of cellular proliferation in the stomach fundus at the end of gestation. The gastric epithelium had well-defined glands composed of parietal, chief and mucous cells just before the birth. The stomach fundus, therefore, were developed morphologically as those of normal adult at fourteen days after birth.

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Effect of High Dose ${\gamma}-irradiation$ on the Physicochemical Properties of Shell Eggs during Storage (고선량 감마선 조사가 신선란의 저장 중 이화학적 특성에 미치는 영향)

  • Moon, Sun-Ae;Song, Kyung-Bin
    • Applied Biological Chemistry
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    • v.43 no.4
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    • pp.260-265
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    • 2000
  • To determine the quality change of the irradiated eggs during storage, fresh shell eggs were irradiated using $^{60}Co$ at 0, 1, 5, 10, 30 kGy and stored for 30 days. The york index, color, pH, viscosity, egg weight, and SDS-PAGE profile of the irradiated eggs were examined. During storage, york index values of the irradiated eggs and the control were decreased and the increase of dose decreased yolk index. However, the yolk index values were increased temporarily at 10 kGy and 30 kGy. The yolk color had a bright yellow with increases in dose level and there was no significant change during storage. The albumen viscosities were decreased with increases in dosage and were decreased during storage. Also, the albumen pH values of the irradiated eggs were higher than that of the control and were increased during storage. The weight losses of eggs were increased during storage and there were no significant changes by dose level. SDS-PAGE profile of the egg white proteins of the shell eggs showed the change in molecular weight distribution and had aggregation pattern as well as degradation. CD and fluorescence spectroscopy study showed changes in the secondary and tertiary structure of egg white proteins by ${\gamma}-irradiation$. Therefore, this study clearly indicates that irradiation dose of eggs should be appropriate to prevent the loss of egg qualities.

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Evaluation of incremental sheet forming characteristics for 3D-structured aluminum sheet - part 2 (3D 구조 알루미늄 판재의 점진판재성형 특성 평가 (제2보))

  • Kim, Young-Suk;Do, Van-Cuong;Ahn, Dae-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.3
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    • pp.1585-1593
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    • 2015
  • 3D-structured (embossed) aluminum sheets have been used in the heat insulation purpose for automative exhaust parts because of increasing their surface areas and stiffness reinforcement imposed in making the embossing pattern. However, there are many restrictions in press forming of the embossed sheet compared with the flat sheet (non-embossed one) because of its difference in the mechanical properties and the geometrical 3-dimensional shape. In this paper we investigated the deformation characteristic of embossed aluminum sheet in the incremental sheet forming process which has frequently used in the design verification and the trial manufacturing of sheet products. The single point incremental forming (SPIF) experiments for the rectangular cone forming using the CNC machine with a chemical wood-machined die and a circular tool shape showed that the formability of the embossed sheet are better than that of the flat sheet in view of the maximum angle of cone forming. This comes from the fact that the embossed sheet between the tool and the elastic die wall is plastically compressed and the flatted area contributes to increase the plastic deformation. Also the tool path along the outward movement from the center showed a better formability than that of the inward movement from the edge. However the surface quality for the tool path along the outward movement evaluated from the surface deflection is inferior than that of the tool path along the inward movement.

Design and Comparison of Superconducting Magnets with Circular Coil Elements for Magnetic Resonance Imaging (원형무코일로 구성된 MRI용 초전도 자석의 설계와 비교)

  • Kim, Yong-Gwon;Hyun, Jung-Ho;Seo, Jeung-Hoon;Kim, Hyug-Gi;Oh, Chang-Hyun
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.48 no.6
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    • pp.57-62
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    • 2011
  • This paper proposed a method which is the three types of magnet model for improving field inhomogeneity of superconducting magnet. The length of coil wire was compared for the optimized current pattern using minimum power methods and field inhomogeneity under the specific simulation condition in case of same magnet field strength about each magnet type field inhomogeneity. Length of wire and field inhomogeneity were compared under the same condition(18 target points, 20cm DSV). According to the simulation results, the smaller target points can reduce the wire length but it can not improve the field inhomogeneity. Length of wire and low field inhomogeneity can not improve in same time. However, small DSV and reducing target points can overcome the these problem. And to conclude, if it processes shimming as reducing target points in case of magnet model which is open to space, about the size of same imaging region it needs a lot of current values(or the length of wire) and decreases field homogeneity but it is useful to get small ROI.

A PHOTOELASTIC STRESS ANALYSIS IN MANDIBULAR DISTAL EXTENSION REMOVABLE PARTIAL DENTURE DESIGNED UNILATERALLY WITH DIFFERENT DIRECT RETAINERS (편측성으로 설계된 하악 유리단 국소의치에서 직접유지장치의 설계 변화에 따른 광탄성 응력 분석에 관한 연구)

  • Son Hong-Suk;Kay Kee-Sung
    • The Journal of Korean Academy of Prosthodontics
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    • v.30 no.1
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    • pp.25-42
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    • 1992
  • The purpose of this study was to analyze the magnitude and distribution of stress using a photoelastic model from a unilateral distal extention removable partial dentures with five kinds of the direct retainers, that is, the bilaterally designed bar clasp of the cross-arch lingual bar and the unilaterally designed bar clasp, circumferential clasp, mini-Dalbo attachment, and telescope retainer. A photoelastic model for mandible was made of the epoxy resin(PL-1) and hardner (PLH-1) with the acrylic resin teeth used and was coated with plastic cement-1 at the lingual surface of the model, and then five kinds of removable partial dentures were set, A unilateral vertical load of about 16Kg was applied on the first molar and the stress pattern of the photoelastic model under each condition was analyzed by the reflective circular polariscope. The following results were obtained: 1. The conventional removable partial denture with the bilaterally cross arch lingual bar produced the most favorable stress distribution on the residual ridge and supporting structure of abutment teeth than the unilaterally designed removable partial dentures. 2. The unilaterally designed removable partial denture with the bar clasp produced the stress distribution on the residual ridge, except sligtly higher stress concentration on the supporting structure of the abutment teeth, similar to the conventional removable partial denture with the bilaterally designed cross arch lingual bar. 3. On the unilaterally designed removable partial dentures, the bar clasp produced greater stress distribution on the residual ridge and supporting structure of the abutment teeth than the circumferential clasp. 4. On the unilaterally designed removable partial dentures, the mimi-Dalbo attachment produced relatively higher stress concentration on the residual ridge, but produced lesser stress concentration on the supporting structure of the abutment teeth than the other direct retainers. 5. On the unilaterally designed removable partial dentures, the telescope retainer produced uniform stress distribution on the residual ridge, but produced higher stress concentration at the root apex of the terminal abutment tooth than the other direct retainers. 6. On the unilaterally designed removable partial dentures the circumferential clasp and telescope retainer produced slightly higher stress concentration on the residual ridge and supporting structure of the abutment teeth than the bar clasp and mini- Dalbo attachment.

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Histopathological Observation of Three Types of Root Resorption Surface in Maxillary Primary Incisors (상악 유절치의 3가지 유형의 치근 흡수면에 관한 병리조직학적 관찰)

  • Na, Hyejin;Son, Hyoju;Song, Jeseon;Kim, Seongoh;Lee, Jaeho;Choi, Hyungjun;Choi, Byungjai
    • Journal of the korean academy of Pediatric Dentistry
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    • v.44 no.3
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    • pp.289-297
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    • 2017
  • Local and general factors have been attributed to root resorption occurred by injuries such as trauma and dental caries that affect periodontal ligament or dental pulp tissue. Pathologic root resorption is different from physiologic root resorption in terms of resorption pattern such as micromorphology of resorption fossae and types of observed cells. Microscopic morphologies and histologic features of physiologic and pathologic root resorption surface of maxillary primary central incisors resulting from trauma and periapical inflammation were observed by scanning electron microscope and light microscope. The morphology of physiologic resorption lacunae was small and oval or circular shape with regularities. The morphology of pathologic resorption lacunae was large and polygonal shape with irregularities compared with the physiologic resorption lacunae. Multinucleated giant cells and mononuclear cells were closely attached to the physiologic and pathologic resorption lacunae, whereas several kinds of mesenchymal cells with numerous inflammatory cells were found in the areas adjacent to the pathologic resorption surface. Compensating cementum formation took place along some of the areas of physiologic and pathologic resorption area resulting from trauma, but could not be observed on pathologic resorption area resulting from periapical inflammation.

A Study on Kinds and Features of Dan(緞) at the End of the Joseon Period (조선 말기 단(緞)의 종류 및 특성에 관한 연구)

  • Lee, Eun-Jin;Cho, Hyo-Sook;Hong, Na-Young
    • Journal of the Korean Society of Costume
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    • v.57 no.3 s.112
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    • pp.37-52
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    • 2007
  • Dan(緞) is satin damask. It was a newly popularized fabrics in the Joseon Period and is one of main traditional fabrics at present. The purpose of this study is to suggest a theoretical basis to name Dan(緞) remains properly by comparing and analyzing the name, pattern, usage, feature, length and width, value of Dan(緞) recorded on documents made at the end of the Joseon Period. The results of this study are as follows. 1. The kinds of Dan(緞) recorded on documents made at the end of the Joseon Period are various. Among fabrics named Dan(緞), were not only silk fabrics but also cotton fabrics, woolen fabrics, mixture fabrics with silk and cotton, and mixture fabrics with cotton and wool. 2. Some newly revealed patterns in the case of Dan(緞) are as follows. The shape of Byeolmun(別紋) is assumed to have originated from that of character symbolizing the Royal Family and developed into a variety of circular ones of dragon, phoenix, flower, or character. O-ho-ro-mun(五葫蘆紋) was five-gourd-shaped patterns that form a circle, turning their narrow mouth to the center, and vines between each two gourds. 3. When considering the usages of Dan(緞), Dan(緞) was used for various detailed usages according to their kinds. But it was't mostly used for underwear. 4. Features have been examined are Godan(庫緞), Mobondan(模本緞), Handan(漢緞), Daedan(大緞), Yangdan(洋緞), Waedan(倭緞), and Geumdan(錦緞). 5. The length and the width of 1 Pil(疋) silk fabrics are various. It is normal for the width being different, but for 1 Pil(疋)'s length being different is very unusual. The width of Dan (緞) was about 47-76.20cm, it was wider than Ju(紬), Cho, Gyeon(絹), Sa(紗), Ra(羅) and Reung(綾). 6. As for the value of each fabric per $10,000cm^2$, Dan(緞) was the most valuable silks at that time. Among them the most valuable Dan(緞) was Udan(羽緞).

A Study on the Impact Fracture of Fragile Materials (취성재료의 충격파괴에 관한 연구 II)

  • 양인영;김택현;정낙규;이상호;김선규
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.14 no.6
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    • pp.1417-1425
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    • 1990
  • In this paper, when the glass plate of fragile material is subjected to impact load, the fracture pattern and the generation phenomenon of cone crack were theoretically clarified by using the analysis method of impulsive stress in the first paper. The numerical analysis results of strain distribution at a distance of 0.1cm, from the impact loading point to 5cm, were compared with experimental results. The main conclusions obtained are as follows; (1) The generation phenomenon of cone crack at the impact fracture of the glass plate can be analytically confirmed by using the three dimensional dynamic theory of elasticity. And the numerical analysis results of strain distribution using this theory are relatively in close agreement with the crack size obtained from the impact fracture experiment. (2) After the stress wave generated at the impact point propagated to the spherical compressive wave, this stress wave reflected from the back surface and reached again at the surface of the plate to the spherical stress wave. Then the generation of cone crack can be confirmed along the stress wave surface. (3) The plate is the thicker, the more is the generation phenomenon of cone crack at the lower impact velocity range (20m/s-35m/s). Because the fracture generate before the maximum tensile stress acting to the glass plate, cone crack was rarely ever generated.