• Structural Engineering and Mechanics
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• 제66권2호
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• pp.273-283
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• 2018

Currently, the dynamic amplification effect of suction is described using the wind vibration coefficient (WVC) of external loads. In other words, it is proposed that the fluctuating characteristics of suction are equivalent to external loads. This is, however, not generally valid. Meanwhile, the effects of the ventilation rate of louver on suction and its WV are considered. To systematically analyze the effects of the ventilation rate of louver on the multi-dimensional WVC of ultra-large cooling towers under suctions, the 210 m ultra-large cooling tower under construction was studied. First, simultaneous rigid pressure measurement wind tunnel tests were executed to obtain the time history of fluctuating wind loads on the external surface and the internal surface of the cooling tower at different ventilation rates (0%, 15%, 30%, and 100%). Based on that, the average values and distributions of fluctuating wind pressures on external and internal surfaces were obtained and compared with each other; a tower/pillar/circular foundation integrated simulation model was developed using the finite element method and complete transient time domain dynamics of external loads and four different suctions of this cooling tower were calculated. Moreover, 1D, 2D, and 3D distributions of WVCs under external loads and suctions at different ventilation rates were obtained and compared with each other. The WVCs of the cooling tower corresponding to four typical response targets (i.e., radial displacement, meridional force, Von Mises stress, and circumferential bending moment) were discussed. Value determination and 2D evaluation of the WVCs of external loads and suctions of this large cooling tower at different ventilation rates were proposed. This study provides references to precise prediction and value determination of WVC of ultra-large cooling towers.

• International Journal of Fluid Machinery and Systems
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• 제8권4호
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• pp.264-273
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• 2015

This presentation describes an experimental approach for the detection of cavitation in hydraulic machines by use of ultrasonic signal analysis. Instead of using the high frequency pulses (typically 1MHz) only for transit time measurement different other signal characteristics are extracted from the individual signals and its correlation function with reference signals in order to gain knowledge of the water conditions. As the pulse repetition rate is high (typically 100Hz), statistical parameters can be extracted of the signals. The idea is to find patterns in the parameters by a classifier that can distinguish between the different water states. This classification scheme has been applied to different cavitation sections: a sphere in a water flow in circular tube at the HSLU in Lucerne, a NACA profile in a cavitation tunnel and two Francis model test turbines all at LMH in Lausanne. From the signal raw data several statistical parameters in the time and frequency domain as well as from the correlation function with reference signals have been determined. As classifiers two methods were used: neural feed forward networks and decision trees. For both classification methods realizations with lowest complexity as possible are of special interest. It is shown that two to three signal characteristics, two from the signal itself and one from the correlation function are in many cases sufficient for the detection capability. The final goal is to combine these results with operating point, vibration, acoustic emission and dynamic pressure information such that a distinction between dangerous and not dangerous cavitation is possible.

• 한국지진공학회논문집
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• 제3권2호
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• pp.1-8
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• 1999

지진의 수평지반운동으로 인해 유체저장탱크에 작용하는 수평하중의 산정에 있어서 유체수심에 대한 탱크저면길이의 비율에 따라 유용하게 사용할 수 있는 근사해법에 대하여 연구하였다 본 연구는 탱크벽체를 강체로 가정하고 구한 속도포텐셜이론을 적용하여 탱크의 지진하중을 산정한다. 유체저장탱크의 수평단면이 연직으로 일정하지만 그 형상은 원형, 직사각형, 불규칙형인 경우로 나누어 해석하였다 우선 주기적 지반운동(조화가진)에 대한 해를 구하고 이를 바탕으로 랜덤지진에 대한 시간영역해를 구하도록 한다. 예제해석결과 지진력은 적용하는 설계응답스펙트럼의 특성 탱크단면형, 수심에 대한 저면길이의 비율등에 따라 차이가 다소있으나 주로 고주파수 유효질량의 관성력이 지배적인 것으로 나타났다. 대체적으로 수심에 대하여 탱크저면길이가 상당히 크거나 작은 경우는 고주파수근사해를 써서 지진력을 효율적으로 산정할 수 있었다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.69-69
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• 2003

Human CD99 is a ubiquitous 32-kDa transmembrane protein encoded by the mic2 gene. The major cellular functions of CD99 protein are related to homotypic cell adhension, apoptosis, vesicular protein transport, and differentiation of thymocytes or T cells. Recently it has been reported that expression of a splice variant of CD99 transmembrane protein (Type I and Type II) increases invasive ability of human breast cancer cells. To understand structural basis for cellular functions of CD99 (Type I), we have initiated studies on hCD99$^{TMcytoI}$ and hCD99$^{cytoI}$ using circular dichroism (CD) and multi-dimensional NMR spectroscopy. CD spectrum of hCD99$^{TMcytoI}$ in the presence of 200mM DPC and CHAPS displayed an existence $\alpha$-helical conformation. The solution structure of hCD99$^{cytoI}$ determined by NMR is composed of one N-terminal $\alpha$-helix, $\alpha$A, two C-terminal short $\alpha$-helix segments, $\alpha$B and $\alpha$C. While $\alpha$A and $\alpha$B are connected by the long flexible loop, $\alpha$B and $\alpha$C connected by type III$\beta$-turn. Although it has been rarely figured out the correlation between structure and functional mechanism of hCD99$^{TMcytoI}$ and hCD99$^{cytoI}$, there is possibility of dimerization or oligomerization. In addition, the feasible mechanism of hCD99$^{cytoI}$ is that it could have intramolecular interaction between the N- and C- terminal domain through large flexible AB loop.

• Bulletin of the Korean Chemical Society
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• 제28권5호
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• pp.730-736
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• 2007

The interaction of a polyoxometal (POM), K6SiW11Co(H2O)O39.10H2O (K6) as a Keggin, with human serum albumin (HSA) was studied by different methods and techniques. Binding studies show two sets of binding sites for interaction of POM to HSA. Binding analysis and isothermal calorimetery revealed that, the first set of binding site has lower number of bound ligand per mole of protein (ν), lower Hill constant (n), higher binding constant (K), more negative entropy (ΔS) and more electrostatic interaction in comparison to the second set of binding site. In addition, differential scanning calorimetery (DSC) and spectrophotometery data showed that, there are two energetic domains. The first domain is less stable (lower Tm and Cp) which corresponds to the tail segment of HSA and another with more stability is related to the head segment of HSA. Polyoxometal also decreases the stability of protein as Tm, secondary and tertiary structure as well as quenching of the fluorescence decrease. On other hand, perturbations in tertiary structure are more than secondary structure.

• Wind and Structures
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• 제20권1호
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• pp.15-36
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• 2015

Characterization of wind flows over a complex terrain, especially mountain-gorge terrain (referred to as the very complex terrain with rolling mountains and deep narrow gorges), is an important issue for design and operation of long-span bridges constructed in this area. In both wind tunnel testing and numerical simulation, a transition section is often used to connect the wind tunnel floor or computational domain bottom and the boundary top of the terrain model in order to generate a smooth flow transition over the edge of the terrain model. Although the transition section plays an important role in simulation of wind field over complex terrain, an appropriate shape needs investigation. In this study, two principles for selecting an appropriate shape of boundary transition section were proposed, and a theoretical curve serving for the mountain-gorge terrain model was derived based on potential flow theory around a circular cylinder. Then a two-dimensional (2-D) simulation was used to compare the flow transition performance between the proposed curved transition section and the traditional ramp transition section in a wind tunnel. Furthermore, the wind velocity field induced by the curved transition section with an equivalent slope of $30^{\circ}$ was investigated in detail, and a parameter called the 'velocity stability factor' was defined; an analytical model for predicting the velocity stability factor was also proposed. The results show that the proposed curved transition section has a better flow transition performance compared with the traditional ramp transition section. The proposed analytical model can also adequately predict the velocity stability factor of the wind field.

• Ocean Systems Engineering
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• 제7권2호
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• pp.121-141
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• 2017

The purpose of this paper is to understand and model the slow current (~2 m/s) effects on the global response of a floating offshore platform in waves. A time-domain numerical simulation of full wave-current-body interaction by a quadratic boundary element method (QBEM) is applied to compute the hydrodynamic loads and motions of a floating body under the combined influence of waves and current. The study is performed in the context of linearized potential flow theory that is sufficient in understanding the leading-order current effect on the body motion. The numerical simulations are validated by quantitative comparisons of the hydrodynamic coefficients with the WAMIT prediction for a truncated vertical circular cylinder in the absence of current. It is found from the simulation results that the presence of current leads to a loss of symmetry in flow dynamics for a tension-leg platform (TLP) with symmetric geometry, resulting in the coupling of the heave motion with the surge and pitch motions. Moreover, the presence of current largely affects the wave excitation force and moment as well as the motion of the platform while it has a negligible influence on the added mass and damping coefficients. It is also found that the current effect is strongly correlated with the wavelength but not frequency of the wave field. The global motion of a floating body in the presence of a slow current at relatively small encounter wave frequencies can be satisfactorily approximated by the response of the body in the absence of current at the intrinsic frequency corresponding to the same wavelength as in the presence of current. This finding has a significant implication in the model test of global motions of offshore structures in ocean waves and currents.

• International Journal of Ocean System Engineering
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• 제2권2호
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• pp.116-138
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• 2012

As in the other fields of mechanics, analytical methods represent an important analysis tool in marine hydrodynamics. The analytical approach is interesting for different reasons : it gives reference results for numerical codes verification, it gives physical insight into some complicated problems, it can be used as a simplified predesign tool, etc. This approach is of course limited to some simplified geometries (cylinders, spheres, ...), and only the case of one or more cylinders, truncated or not, will be considered here. Presented methods are basically eigenfunction expansions whose complexity depends on the boundary conditions. The hydrodynamic boundary value problem (BVP) is formulated within the usual assumptions of potential flow and is additionally simplified by the perturbation method. By using this approach, the highly nonlinear problem decomposes into its linear part and the higher order (second, third, ...) corrections. Also, periodicity is assumed so that the time dependence can be factorized i.e. the frequency domain formulation is adopted. As far as free surface flows are concerned, only cases without or with small forward speed are sufficiently simple to be solved semi-analytically. The problem of the floating body advancing in waves with arbitrary forward speed is far more complicated. These remarks are also valid for the general numerical methods where the case of arbitrary forward speed, even linearized, is still too difficult from numerical point of view, and "it is fair to say that there exists at present no general practical numerical method for the wave resistance problem" [9], and even less for the general seakeeping problem. We note also that, in the case of bluff bodies like cylinders, the assumptions of the potential flow are justified only if the forward speed is less than the product of wave amplitude with wave frequency.

• 지질공학
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• 제31권3호
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• pp.257-267
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• 2021

Constraints on the structure and composition of the active layer are important for understanding permafrost evolution. Soil convection owing to repeated moisture-induced freeze-thaw cycles within the active layer promotes the formation of self-organized patterned ground. Here we present the results of ground penetrating radar (GPR) surveys across a selected sorted circle near King Sejong Station, Antarctica, to better delineate the active layer and its relation to the observed patterned ground structure. We acquire GPR data in both bistatic mode (common mid-points) for precise velocity constraints and monostatic mode (common-offset) for subsurface imaging. Reflections are derived from the active layer-permafrost boundary, organic layer-weathered soil boundary within the active layer, and frozen rock-fracture-filled ice boundary within the permafrost. The base of the imaged sorted circle possesses a convex-down shape in the central silty zone, which is typical for the pattern associated with convection-like soil motion within the active layer. The boundary between the central fine-silty domain and coarse-grained stone border is effectively identified in a radar amplitude contour at the assumed active layer depth, and is further examined in the frequency spectra of the near- and far-offset traces. The far-offset traces and the traces from the lower frequency components dominant on the far-offset traces would be associated with rapid absorption of higher frequency radiowave due to the voids in gravel-rich zone. The presented correlation strategies for analyzing very shallow, thin-layered GPR reflection data can potentially be applied to the various types of patterned ground, particularly for acquiring time-lapse imaging, when electric resistivity tomography is incorporated into the analysis.

• Journal of Microbiology and Biotechnology
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• 제30권12호
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• pp.1905-1911
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• 2020

Homoserine dehydrogenase (HSD) catalyzes the reversible conversion of ʟ-aspartate-4-semialdehyde to ʟ-homoserine in the aspartate pathway for the biosynthesis of lysine, methionine, threonine, and isoleucine. HSD has attracted great attention for medical and industrial purposes due to its recognized application in the development of pesticides and is being utilized in the large scale production of ʟ-lysine. In this study, HSD from Bacillus subtilis (BsHSD) was overexpressed in Escherichia coli and purified to homogeneity for biochemical characterization. We examined the enzymatic activity of BsHSD for ʟ-homoserine oxidation and found that BsHSD exclusively prefers NADP+ to NAD+ and that its activity was maximal at pH 9.0 and in the presence of 0.4 M NaCl. By kinetic analysis, Km values for ʟ-homoserine and NADP+ were found to be 35.08 ± 2.91 mM and 0.39 ± 0.05 mM, respectively, and the Vmax values were 2.72 ± 0.06 μmol/min-1 mg-1 and 2.79 ± 0.11 μmol/min-1 mg-1, respectively. The apparent molecular mass determined with size-exclusion chromatography indicated that BsHSD forms a tetramer, in contrast to the previously reported dimeric HSDs from other organisms. This novel oligomeric assembly can be attributed to the additional C-terminal ACT domain of BsHSD. Thermal denaturation monitoring by circular dichroism spectroscopy was used to determine its melting temperature, which was 54.8℃. The molecular and biochemical features of BsHSD revealed in this study may lay the foundation for future studies on amino acid metabolism and its application for industrial and medical purposes.