• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.33-43
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• 2015

GAM-1 and GAM-2, two themostable glucoamylases from Aspergillus niger B-30, possess different molecular masses, glycosylation, and thermal stability. In the present study, the effects of additives on the thermal inactivation of GAM-1 and GAM-2 were investigated. The half-lives of GAM-1 and GAM-2 at 70℃ were 45 and 216 min, respectively. Data obtained from fluorescence spectroscopy, circular dichroism spectroscopy, UV absorption spectroscopy, and dynamic light scattering demonstrated that during the thermal inactivation progress, combined with the loss of the helical structure and a majority of the tertiary structure, tryptophan residues were partially exposed and further led to glucoamylases aggregating. The thermal stability of GAM-1 and GAM-2 was largely improved in the presence of sorbitol and trehalose. Results from spectroscopy and Native-PAGE confirmed that sorbitol and trehalose maintained the native state of glucoamylases and prevented their thermal aggregation. The loss of hydrophobic bonding and helical structure was responsible for the decrease of glucoamylase activity. Additionally, sorbitol and trehalose significantly increased the substrate affinity and catalytic efficiency of the two glucoamylases. Our results display an insight into the thermal inactivation of glucoamylases and provide an important base for industrial applications of the thermally stable glucoamylases.

• Science of Emotion and Sensibility
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• v.10 no.3
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• pp.393-402
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• 2007

The purpose of this study is to provide the design direction based on consumer sensitivity through the structure between product design preferences - sensitivity image - design elements. For the purpose, we selected men's shaver products for this study subject and collected 164 shavers' pictures released between 2001-2007 years. Then, we carried out a pilot test for collection of sensitivity images about shavers, made a survey using semantic differential method and analyzed the survey. According the result, consumers preferred the sensitivity images "luxury, attractive, stable", design elements satisfied the preference images were "form of body is not a circular arcs or a polygon, material is steel, button is push style, and a color of body is not brown." This study can provide a base of the causal relationship between design preferences - sensitivity image - design elements and a design process to predict consumer sensitivity-oriented design.

• Fire Science and Engineering
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• v.26 no.3
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• pp.79-84
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• 2012

In this study thermal characteristics of heat-responsive element considering conduction, convection and rate of change of element using Response Time Index (RTI) applied to sensitivity test of sprinkler head at home and aborad are theoretically investigated. Analytic solution of temperature distributions with radial direction and time is obtained form energy transport equations, non-homogeneous 2th order partial differential equation, applying to constant wall temperature and symmetric condition in order to analyze thermal characteristics of heat-responsive element for circular cylindrical geometry. Base on the results, the analytic method of this study is fundamental data to practical use for sensitivity test of sprinkler head and design of heat-responsive element.

• Magazine of the Korean Society of Agricultural Engineers
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• v.30 no.1
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• pp.38-49
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• 1988

This paper presented as follows results of laboratory model tests with various shaped footings on soil bed reinforced with the strips on the base of behaviour of soil structure according to the loads and triaxial test results reinforced with geotextiles. Their parameters studied were the effects on the bearing capacity of a footing of the first layer of reinforcement, horizontal and vertical spacing of layers, number of layers, tensile strength of reinforcement and iclination load to the vertical 1.Depending on the strip arrangement, ultimate bearing capacity values could be more improved than urreinforced soil and the failure of soil was that the soil structure was transfered from the macrospace to microspase and its arrangement, from edge to edge to face to face. 2.The reinforcement was produced the reinforcing effects due to controlling the value of factor of one and permeable reinforcement was never a barrier of drainage condition. 3.Strength ratio was decreased as a linear shape according to increment of saturation degree of soil used even though at the lower strength ratio, the value of M-factor was rot influenced on the strength ratio but impermeable reinforcement decreased the strength of bearing capacity. 4.Ultimate bearing capacity under the plane-strain condition was appeared a little larger than triaxial or the other theoretical formulars and the circular footing more effective. 5.The maximum reinforcing effects were obtained at U I B=o.5, B / B=3 and N=3, when over that limit only acting as a anchor, and same strength of fabric appeared larger reinforcing effects compared to the thinner one. 6.As the LDR increased, more and more BCR occurred and there was appeared a block action below Z / B=O.5, but over the value, decrement of BCR was shown linear relation, and no effects above one. 7.The coefficient of the inclination was shown of minimum at the three layers of fabrics, but the value of H / B related to the ultimate load was decreased as increment of inclination degree, even though over the value of 4.5 there wasn't expected to the reinforcing effects As a consequence of the effects on load inclination, the degree of inclination of 15 per cent was decreased the bearing capacity of 70 per cent but irnproved the effects of 45 per cent through the insertion of geotextile.

• Journal of the Korean Institute of Landscape Architecture International Edition
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• no.1
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• pp.89-101
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• 2001

The purpose of this study ins to verify the characteristic location of Byungyoung Castle, physical type, inside spatial organization, and the scheme of Byungyoung Castle. The study utilizes historic literature, ancient maps related to Byungyoung Castle, topographical and cadastral maps which were published under the rule of Japanese Imperialism Castle, topographical and cadastral maps which were published under the rule of Japanese Imperialism and the topographical maps which were made recently by National Geographic Institute with various scales. The methodology of the study is to interpret the contents from the historic literature on the site map. The methodology of the study is to interpret the contents from the historic literature on the site map. The result of the study is as follows; Byungyoung Castle does duty as a defensive base for the entire country and has a specific character of location that has the dual function of a mountain fortress for national defense and of a village fortress for the town. Byungyoung Castle has four gates on four sides and has a oval shape very close to a circular form. The road construction inside the castle is composed basically of a cross shape. Byungyoung is located in the northwest area of this major road system. The private houses that lie along the north-south road are build up at the core area of the lower level and the town market built up around the south gate becomes the heart of life for the people. Schematically, it has the same pattern as regular village fortress, in that the houses for the guests and the houses for the public office are arranged to the east and the west. It is considered that there is certain functional parallel between Byungyoug Castle and Ulsan castle because there are no facilities for sacrificial rites no institutional budding.

• Journal of Educational Research in Mathematics
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• v.18 no.2
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• pp.263-281
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• 2008

This study is to provide a base for discussions on teaching and teaming of radian through a theoretical analysis of it. Radian possesses two-fold comprehensive properties of measurement as a magnitude and a pure number. As a magnitude of an angle, it has some theoretical advantages in mathematics and in physics, in spite of its non-superiority to other angular measures in practical sense. As a pure number, it has some advantages in that it simplifies theoretical developments of trigonometric functions and justifies omitting the unit in the calculations and final expressions in physics. Radian should be taught and learnt with an appreciation of the advantages of the two-fold properties. Activities to measure angles from various viewpoints may be helpful for this. Students' awareness of the advantages of radian needs to be stimulated and deepened repeatedly as related content appears.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.6
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• pp.531-540
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• 2007

While determination of the solid propellant burning rates by ultrasound, it has been reported that the frequent data scatters were caused by two major factors; 1) variation in the acoustical properties, and 2) non-linear burning of a solid propellant sample under investigation. This work is carried out for the purpose of investigating the effect of non-linear burning of solid propellant samples. Specifically, we propose an ultrasonic measurement model that can predict the reflections from solid propellant surfaces with non-linear burning by the combination of two ingredients; 1) a pulse-echo ultrasonic measurement model for a planar, circular reflector imbedded in the second medium in an immersion set-up, and 2) an efficient model of non-linear burning surfaces with a number of small, planar circles. Then, we demonstrate the capability of the proposed measurement model by simulation of the surface echo signals from four different burning surfaces that have been generated by the combination of two factors; the base shape (flat or paraboloidal) and the surface roughness (perfectly smooth or randomly rough). From the simulation presented here, we can confirm the fact that the non-linear burning of the propellant can cause the waveform change of the burning surface echo and the corresponding spectrum variation.

• Korean Journal of Microbiology
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• v.53 no.4
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• pp.351-353
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• 2017

Chryseobacterium sp. strain T16E-39, isolated from roots of a tomato plant, promotes plant growth and suppresses phytophthora blight and bacterial wilt diseases. The complete genome of strain T16E-39 consists of a circular chromosome with 4,872,888 base pairs with a G + C content of 35.22%. The genome includes 4,289 coding sequences, 15 rRNAs, and 71 tRNAs. We detected genes involved in phosphate solubilization, phytohormone regulation, antioxidant activity, chitin degradation, and the type IX secretion system (T9SS) that may be related to growth promotion and disease suppression in plants.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 1997.06a
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• pp.73-101
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• 1997

Baculoviruses are characterized by large double-stranded circular DNA genomes and rod-shaped enveloped virions. Bombyx mori nucleopolyhedrovirus(BmNPV) is a major pathogen, which causes severe damage in sericulture. Currently, BmNPV is recogtnized as an improtant tool in molecular biology, especially for expression of useful genes in B.mori cells and silkworm larvae. Our laboratories have focused on the studies of the molecular mechanisms of BmNPV replication and the application of BmNPV to agriculture and medicine. The entire nucleotide sequence of the BmNPV genome has recently determined. The BmNPV genome possessed 135 putative genes and 7 homologous repeated sequence (hrs) regions. Relatively little space, a few to a few hundred base-pairs, was observed between the open reading frames and hrs. Termination codons often overlapped. These results showed a compactly packde BmNPV genome. Based on comparative sequence analyses, we speculated that the ancestor of BmNPV was a baculovirus similar to Autographa californica NPV(AcNPV). The function of the BmNPV genes were characterized by gene deletion analysis; p35 was found to be involved in blocking apoptosis and cysteine proteinase was found to be involved in horizontal virus transmission by degrading viral-infected larval host. By AcNPV and BmNPV coinfection experiments, we identified a BmNPV gene involved in expanding host specificity of AcNPV. The identified gene was likely encoded a DNA helicase based on the amino acid sequence analysis; a few amino acid substitutions in the putative DNA helicase gene resulted in the expansion of host range of AcNPV. These findings indicate that BmNPV evolved within a short period from an AcNPV-like ancestral virus due to rapid evolution including specific amino acid substitutions and gene deletions/insertions.

• The Journal of Engineering Geology
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• v.31 no.3
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• pp.257-267
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• 2021

Constraints on the structure and composition of the active layer are important for understanding permafrost evolution. Soil convection owing to repeated moisture-induced freeze-thaw cycles within the active layer promotes the formation of self-organized patterned ground. Here we present the results of ground penetrating radar (GPR) surveys across a selected sorted circle near King Sejong Station, Antarctica, to better delineate the active layer and its relation to the observed patterned ground structure. We acquire GPR data in both bistatic mode (common mid-points) for precise velocity constraints and monostatic mode (common-offset) for subsurface imaging. Reflections are derived from the active layer-permafrost boundary, organic layer-weathered soil boundary within the active layer, and frozen rock-fracture-filled ice boundary within the permafrost. The base of the imaged sorted circle possesses a convex-down shape in the central silty zone, which is typical for the pattern associated with convection-like soil motion within the active layer. The boundary between the central fine-silty domain and coarse-grained stone border is effectively identified in a radar amplitude contour at the assumed active layer depth, and is further examined in the frequency spectra of the near- and far-offset traces. The far-offset traces and the traces from the lower frequency components dominant on the far-offset traces would be associated with rapid absorption of higher frequency radiowave due to the voids in gravel-rich zone. The presented correlation strategies for analyzing very shallow, thin-layered GPR reflection data can potentially be applied to the various types of patterned ground, particularly for acquiring time-lapse imaging, when electric resistivity tomography is incorporated into the analysis.