• 약학회지
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• 제59권4호
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• pp.151-157
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• 2015

The purpose of this study is to demonstrate how lead compounds are best optimized with the application of in silico QSAR and PBPK modeling at the early drug discovery stage. Several predictive QSAR models such as $IC_{50}$ potency model, intrinsic clearance model and brain penetration model were built and applied to a set of virtually synthesized library of the BACE1 inhibitors. Selected candidate compounds were also applied to the PBPK modeling for comparison between the predicted animal pharmacokinetic parameters and the observed ones in vivo. This novel lead optimization strategy using QSAR and PBPK modelings could be helpful to expedite the drug discovery process.

• Journal of Microbiology and Biotechnology
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• 제6권5호
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• pp.309-314
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• 1996

Alcaligenes sp. SH-69 can synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from a single carbon source such as glucose. To clone the phaC gene from Alcaligenes sp. SH-69, a polymerase chain reaction was performed using the oligomers synthesized based on the conserved regions of the phaC genes from other bacteria. A PCR product (550 bp) was partially sequenced and the deduced amino acid sequence was found to be homologous to that of the phaC gene from Alcaligenes eutrophus. Using the PCR fragment Southern blotting of Alcaligenes sp. SH-69 genomic DNA digested with several restriction enzymes was carried out. To prepare a partial genomic library, about 5-Kb genomic DNA fragments digested with EcoRI, which showed a positive signal in the Southern blotting, were eluted from an agarose gel, ligated with pUC19 cleaved with EcoRI, and transformed into Escherichia coli. The partial library was screened using the PCR fragment as a probe and a plasmid, named pPHA11, showing a strong hybridization signal was selected. Restriction mapping of the insert DNA in pPHA11 was performed. Cotransformation into E. coli of the plasmid pPHA11 and the plasmid pPHA21 which has phaA and phaB from A. eutrophus resulted in turbid E. coli colonies which are indicative of PHA accumulation. This result tells us that the Alcaligenes sp. SH-69 phaC gene in the pPHA11 is functionally active in E. coli and can synthesize PHA in the presence of the A. eutrophus phaA and phaB genes.

• 한국도서관정보학회지
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• 제41권3호
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• pp.205-224
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• 2010

본 연구의 목적은 음악자료 중 1차자료로서 모든 음악활동의 기초가 되는 악보자료의 활용을 위한 효율적인 관리의 기초를 마련하기 위한 것이다. 이를 위해 악보자료에 대해 문헌연구를 통해 악보자료 관리에 대하여 선정 및 수집 조직 이용서비스에 대한 제반사항을 고찰하였다. 그리고 이를 기초로 현재 대전 충남지역 대학도서관의 악보자료 관리현황에 대해 조사한 후, 현재 악보자료 관리의 문제점을 파악하고 이후의 효율적인 관리를 위한 과제를 제시하였다.

• 펄프종이기술
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• 제45권1호
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• pp.1-5
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• 2013

Printing papers published in between 1950's and 1990's were treated with three methods such as distilled water washing, $CaCO_3$ solution washing and methyl cellulose solution coating for improving their conservational properties. Accelerated aging with $80^{\circ}C$ and 80% RH for 14 days was applied to the testing papers. Results showed that distilled water and $CaCO_3$ washing kept increased pH even after accelerated aging, but did not improve folding endurances for 1950's-60's papers. Methyl cellulose treatment did not increased pH of the old papers, but increased folding endurances remarkably for 1950's-60's papers even after accelerated aging. It suggests that methyl cellulose treatment after $CaCO_3$ washing should give improvements both in pH and folding endurance.

• 농업과학연구
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• 제42권1호
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• pp.7-13
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• 2015

Ninety genes randomly selected from tobacco whitefly (Bemisia tabaci) cDNA library was studied for selecting target gene in order to control of tobacco whitefly using TRV-VIGS vector (tobacco rattle virus-virus induced gene silencing vector) with RNAi. First of all, the occurrence of B. tabaci adult according to agro-infiltration of TRV was no significant difference. And that of TRV inserted tobacco whitefly cDNA showed a significant difference in each sample. P CV and N CV sample were more than 80% could be confirmed in 5 samples, for example, wh11, wh36, wh46, wh50 and wh71. Lastly, the occurrence of nymph and egg also showed a significant difference in each sample. That could be confirmed in 11 samples, for example, wh01, wh09, wh10, wh15, wh16, wh23, wh24, wh48, wh64 and wh66. In case of wh46, wh50 and wh71 sample could be confirmed that occurrence of B. tabaci adult was many, but occurrence of B. tabaci nymph and egg was a little. So sample showed a physioecological good effect to control of whitefly need to be investigated variation of gene expression in whitefly body using qRT-PCR through individual test.

• Journal of Plant Biotechnology
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• 제4권2호
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• pp.59-65
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• 2002

ADP-glucose pyrophosphorylase (AGPase) catalyzes the key regulatory step in starch biosynthesis. Two cDNA clones encoding AGPase subunits were isolated from the leaf cDNA library of Chinese cabbage (Brassica campestris L. spp. pekinensis). One was designated as BCAGPS for the small subunit and the other as BCAGPL for the large subunit. Both cDNAs have uninterrupted open reading frames deriving 57 kDa and 63 kDa polypeptides for BCAGPS and BCAGPL, respectively, which showed significant similarity to those of other dicot plants. Also, However, the deduced amino acid sequence of BCAGPL has a unique feature. That is, it contains two regions (Rl and R2) lacking in all other plant enzymes. This is the first report of BCAGPL containing Rl and R2 among plant large subunits as well as small subunits. From the genomic Southern analysis and BAC library screening, we inferred the genomic status of BCAGPS and BCAGPL gene.

• 정보관리연구
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• 제35권2호
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• pp.41-55
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• 2004

본 연구에서는 딸림자료 중 특히, 오디오 테이프 형태의 자료를 디지털화하여 멀티미디어 데이터베이스를 구축함으로써 이용자의 이용편의와 자료관리의 효율성을 제고하는 것에 대한 방안에 대해 논의하고자 한다. 이를 위해 본고에서는 딸림자료의 관리현황, 오디오 데이터 포맷의 종류, 데이터 포맷변환, 관리 및 이용방안 등을 살펴보았다. 또한, 딸림자료의 멀티미디어 데이터베이스 구축을 통한 기대효과 및 문제점을 제시하였다.

• 정보관리연구
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• 제36권2호
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• pp.25-44
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• 2005

정보의 홍수 속에서 개인이 생산하고 활용하고 있는 개인정보도 마찬가지로 스스로 찾기 어려울 만큼 그 정보량이 거대해지고 그 형태가 다양해지고 있다. 따라서 개인정보의 효율적 관리는 필수 불가결한 사항이 되었다. 이에 본 연구에서는 개인 컴퓨터의 디지털 정보자료를 중심으로 한 개인 정보의 효율적 관리방안으로서 Desktop 검색도구의 활용을 제안하였고, Desktop 검색도구의 주요 특성과 성능에 대한 비교 분석을 통해 문제점 및 개선방안을 제시하였다.

• 정보관리연구
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• 제40권3호
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• pp.99-123
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• 2009

KESLI 컨소시엄의 핵심 포인트는 참가기관의 참여도를 높여 응집력과 신뢰성을 증진시킴으로써 출판사의 지속적인 가격인상에 공동으로 대처하고, 국내 각급 기관에 분산 소장된 학술정보를 공동으로 활용할 수 있는 기반을 제공함으로써 참가기관에 실익을 주는 것이다. 본 연구에서는 향후 컨소시엄 참가기관이 공통적으로 적용받을 수 있는 합리적이고 발전적인 컨소시엄 운영모델을 마련하기 위한 전제로써 문헌연구, 설문지 조사와 패널토론을 통해 컨소시엄 참가기관의 전체의견을 수렴하여 KESLI 컨소시엄의 당면과제를 도출하여 제시하고자 한다.

• 농업과학연구
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• 제46권3호
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• pp.471-488
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• 2019

Random genes were screened in two transforming ways to investigate the new genes of a ladybug using the Harmonia axyridis cDNA library stock cell cloned in the LITMUS 28i vector in a previous study. Phenotypic variation was observed after injection of the synthesized double-stranded RNA through the in vitro transcription process. The cDNA library of H. axyridis was transformed into E. coli $DH5{\alpha}$ and 10B competent cells by heat shock. Analysis of the nucleotide sequences of the 42 clones with the insert DNAs revealed that 21 clones were homologous with the genes of insects, and only one clone had a gene from H. axyridis. Thirteen of the 21 insect genes were homologous with genes from coleopteran insects. Fourteen genes were selected, which were identified by the gene screening results, and were synthesized as double-stranded RNA through in vitro transcription. One microgram of the synthesized double-stranded RNA between segments T1 and T2 were injected using a syringe into each anesthetized fourth larvae which were under 2 days old. As a result, a phenotypic variation appeared in the larva injected with the two genes. While the eggs of H. axyridis injected with distilled water hatched out three days after oviposition, the eggs of H. axyridis injected with dsHma 06 did not hatch but become shrivel a week after oviposition. Most of the H. axyridis injected with dsHma 08 died and were unable to complete the pupation or eclosion during ecdysis.