• Journal of Korean Library and Information Science Society
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• v.47 no.3
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• pp.139-165
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• 2016

In this study, the basic operational plan for Chungnam Representative Library was established to define the function, roles, and operating strategies of the Chungnam Representing Library. A perception survey was conducted among public library users in Chungcheongnam-do and visitors to the provincial government building. Based from the results, Chungnam Representing Library's roles in collection, organization, preservation, and provision of comprehensive library resources in the city and province was shown to be the highest at 25.65%. Moreover, when respondents were asked about their preferred data resource type for collection and management of the Chungnam Representing Library, printed data emerged as the most preferred with an average of 3.96. Depending on the theme, literature was the most preferred, resulting to an average of 4.27. Lastly, when respondents were asked about the type of service that they expect from Chungnam Representing Library, a library that provides good books from various areas and knowledge information was the most demanded at 27.79%.

• Entomological Research
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• v.48 no.5
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• pp.384-389
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• 2018

Aphis gossypii Glover is an important insect pest that functions as a viral vector and mediates approximately 45 different viral diseases. As part of a strategy for control of A. gossypii, we investigated the functions of genes using RNAi. To this end, a cDNA library was constructed for various genes and for selecting appropriate targets for RNAi mediated silencing. The cDNA library was constructed using the Gateway cloning system with site-specific recombination of bacteriophage ${\lambda}$. It was used to carry out single step cloning of A. gossypii cDNAs. As a result, a cDNA library with a titer of $8.4{\times}10^6$ was constructed. Since the sequences in this library carry att sites, they can be cloned into various binary vectors. This library will be of value for various studies. For later screening of selected genes, it is planned to clone the library into virus-induced gene silencing (VIGS) vectors, which makes it possible to analyze gene function and allow subsequent transfection of plants. Such transfection experiments will allow testing of RNAi-induced insecticidal activity or repellent activity to A. gossypii, and result in the identification of target genes. It is also expected that the constructed cDNA library will be useful for analysis of gene functions in A. gossypii.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.31 no.4
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• pp.191-212
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• 2020

In this study, a study on the cooperative system of the Chungnam Library's joint preservation data center and a plan for the construction of the use service was proposed based on the analysis of the current status of the preservation library of the Chungcheongnam-do public library. In order to establish a cooperative plan for the joint preservation archive and to promote the provision of services, the current state of the collection of 62 public libraries in Chungnam-do and the status of the preservation archives that are operating separately were identified. As a result of the study, it was found that all matters related to the operation of the joint preservation archive should be made through agreements or consultations between the participants, and the operation of the joint preservation archive should also be made under a systematic cooperation system between the participants. To this end, it is necessary to establish a cooperative system for the Chungnam Library Joint Preservation Archives, and to form and operate the Joint Preservation Archives Steering Committee, a consultative body (cooperation network). In addition, all matters related to the operation of the Joint Preservation Archive, such as transfer criteria, must be regulated and implemented through consultation with the Joint Preservation Archive Steering Committee.

• Radiation Oncology Journal
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• v.23 no.1
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• pp.43-50
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• 2005

Purpose : A number of genes and their products are Induced early or late following exposure of cells to ionizing radiation. These radiation-Induced genes have various effects on irradiated cells and tissues. Suppression subtractive hybridization (SSH) based on PCR was used to Identify the differentially expressed genes by radiation in cervix carcinoma cells. Materials and Methods : Total RNA and poly $(A)^+$ mRNA were Isolated from Irradiated and non-irradiated HeLa cells. Forward- and reverse-subtracted cDNA libraries were constructed using SSH. Eighty-eight clones of each were used to randomly select differentially expressed genes using reverse Northern blotting (dot blot analysis). Northern blotting was used to verify the screened genes. Results : Of the 17t clones, 10 genes in the forward-subtracted library and 9 genes In the reverse-subtracted library were identified as differentially expressed radiation-induced genes by PCR-select differential screening. Three clones from the forward-subtracted library were confirmed by Northern blotting, and showed increased expression in a dose-dependent manner, including a telomerase catalytic subunit and sodium channel-like protein gene, and an ESTs (expressed sequence tags) gene. Conclusion : We Identified differentially expressed radiation-induced genes with low-abundance genes with SSH, but further characterization of theses genes are necessary to clarify the biological functions of them.

• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.51-55
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• 2005

Many cloning vectors in which cDNAs can be inserted to the sense orientation have been developed. Uni-ZAP XR vector (Stratagene) should contain clones that are oriented to sense direction with respect to T3 RNA polymerase primer. Unexpectedly, large portions of cDNAs in Chinese cabbage cDNA library showed unusual insertions, antisense orientation and a hybrid of two different clones. Using two clones, 4H03 and 53-B10, derived from different cDNA libraries, we proposed and demonstrated the possibility of unusual-construct formation by in vitro translation and northern blot analysis. The 4H03 clone was inserted with inverse direction, and its transcript and translation product could be produced by T7 RNA polymerase, indicating that this clone is definitely inserted into inverse orientation. The 53-B10 that contains two independent genes was turned out to be a hybrid in which two genes are inserted to opposite direction each other. All unusual constructs might be due to the presence of small fragments of DNA, like adapter. However, the mechanism underlined the formation of unusual constructs is still remain to be solved.

• Journal of the Korean Society for Library and Information Science
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• v.54 no.4
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• pp.27-51
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• 2020

Regional representative libraries are installing joint preservation archives to solve the problem of lack of preservation space in libraries. This study conducted a survey of public library librarians through a questionnaire survey and an FGI method in order to prepare specific operational policies and implementation plans for the Chungnam Library Joint Preservation Archive, which is a regional representative library. Based on this, an operation policy and implementation plan suitable for the common preservation and use of printed and digital data, which is the goal of the Common Preservation Archive, was proposed. As a result of the study, first, the joint preservation library of the Chungnam Library should first transfer books and serials along with ownership in consideration of the condition and demand of the preservation space of participating libraries. Second, it is necessary to establish an operation management system that can be used by all participating libraries in order to quickly and conveniently operate the joint preservation data center and connect it with a cloud-based integrated data management system. Third, a plan for digitizing and archiving data that was judged to be digitized in the Common Preservation Archive was proposed.

• Journal of the Korean Society for Library and Information Science
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• v.50 no.4
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• pp.283-310
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• 2016

The present study aims to develop measures to enhance user service at Chungnam Representative Library by establishing Chungnam Representative Library Operation Master Plan. To this end, I conducted a literature review, analysis of services of other representative libraries, and a survey. The study results suggested six measures for user service enhancement. The first measure is space-specific programs, such as children's materials room, general materials room, electronic materials room, auditorium, multipurpose room, outdoor stage, exhibition room, and book café. The second measure is the services specific user types, such as children, adolescents, adults, the disabled, and multicultural families. The third measure is information service specific for information type, such as print material service and digital material service. The fourth measure is the service for the Information-Poor including the expansion of mobile library service, the service for information users in special environment, and the development of mobile library rules. Fifth measure is information service for Chungcheongnam-do residents, such as Baekje Studies specialized service that incorporates local characteristics, specialized information service for Chungcheong Studies and local materials, and information service for administrative and policy materials. The last measure is the measure to secure initial users and early stage service enhancement.

• Journal of Korean Library and Information Science Society
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• v.21
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• pp.87-115
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• 1994

The aim of this research is to evaluate the library area of the 7 self-evaluated universities in 1994, and to make them compete one another and go into a better stage of library development. The universities evaluated were Pusan National University, KyungBook National University, Seoul National University, Chonnam National University, Chonbuk National University, Chungnam National University and Pohang University of Science and Technology.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.116-122
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• 2011

In this study, we report the generation and analysis of 1,392 expressed sequence tags (ESTs) from Korean Stewartia (Stewartia koreana Nakai). A cDNA library was generated from the young leaf tissue and a total of 1,392 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. Finally, 1,301 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 100 nucleotides. A total of 893 unigene, consisting of 150 contigs and 743 singletons, was identified after assembling. Also, we identified 95 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 65% of ESTs were homologous with known function and 11.6% of ESTs were matched with putative or unknown function. The remaining 23.2% of ESTs showed no significant similarity to any protein sequences found in the public database. Annotation based searches against multiple databases including wine grape and populus sequences helped to identify putative functions of ESTs and unigenes. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Stewartia and provided for the useful tools as a genetic resource.

• Journal of the Korean Society for Library and Information Science
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• v.57 no.4
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• pp.49-74
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• 2023

This study aimed to establish the 2nd Comprehensive Library Development Plan (2024~2028) for Chungnam Library as a metropolitan representative library to enhance the quality of life for the province's residents through library service development and provide comprehensive and systematic support for public libraries in the province. To achieve this, a diagnosis and analysis of libraries in Chungcheongnam-do, an analysis of the policy environment in Chungcheongnam-do, and a future policy environment outlook for libraries were conducted. Ultimately, the study proposed the mission, vision, goals, implementation strategies, and action plans for the 2nd Comprehensive Library Development Plan for Chungcheongnam-do. Firstly, the mission was proposed as "Leading the future 'knowledge and cultural values' by embracing the people of Chungcheongnam-do." Secondly, the vision was suggested as "An inclusive space that leads the knowledge and culture of the future." The goals include: 1) Creating an inclusive library environment accessible to everyone, 2) Providing library services that embrace the community, 3) Operating the library to realize knowledge and cultural values, 4) Developing library infrastructure to lead the future, and 5) Strengthening community exchange and cooperation networks.