• Journal of Microbiology and Biotechnology
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• v.29 no.8
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• pp.1212-1220
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• 2019

The study of metabolomics in natural products using the diverse analytical instruments including GC-MS, LC-MS, and NMR is useful for the exploration of physiological and biological effects and the investigation of drug discovery and health functional foods. Cordyceps militaris has been very attractive to natural medicine as a traditional Chinese medicine, due to its various bioactive properties including anti-cancer and anti-oxidant effects. In this study, we analyzed the metabolite profile in 50% ethanol extracts of C. militaris fruit bodies from three development periods (growth period, matured period, and aging period) using $^1H-NMR$, and identified 44 metabolites, which are classified as 16 amino acids, 10 organic acids, 5 carbohydrates, 3 nucleotide derivatives, and 10 other compounds. Among the three development periods of the C. militaris fruit body, the aging period showed significantly higher levels of metabolites including cordycepin, mannitol (cordycepic acid), and ${\beta}-glucan$. Interestingly, these bioactive metabolites are positively correlated with antitumor growth effect; the extract of the aging period showed significant inhibition of HepG2 hepatic cancer cell proliferation. These results showed that the aging period during the development of C. militaris fruit bodies was more highly enriched with bioactive metabolites that are associated with cancer cell growth inhibition.

• Investigative Magnetic Resonance Imaging
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• v.25 no.2
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• pp.118-128
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• 2021

Purpose: To investigate the value of MR textural analysis, including use of diffusion-weighted imaging (DWI) to differentiate malignant from benign soft-tissue tumors on 3T MRI. Materials and Methods: We enrolled 69 patients (25 men, 44 women, ages 18 to 84 years) with pathologically confirmed soft-tissue tumors (29 benign, 40 malignant) who underwent pre-treatment 3T-MRI. We calculated MR texture, including mean, standard deviation (SD), skewness, kurtosis, mean of positive pixels (MPP), and entropy, according to different spatial-scale factors (SSF, 0, 2, 4, 6) on axial T1- and T2-weighted images (T1WI, T2WI), contrast-enhanced T1WI (CE-T1WI), high b-value DWI (800 sec/mm²), and apparent diffusion coefficient (ADC) map. We used the Mann-Whitney U test, logistic regression, and area under the receiver operating characteristic curve (AUC) for statistical analysis. Results: Malignant soft-tissue tumors had significantly lower mean values of DWI, ADC, T2WI and CE-T1WI, MPP of ADC, and CE-T1WI, but significantly higher kurtosis of DWI, T1WI, and CE-T1WI, and entropy of DWI, ADC, and T2WI than did benign tumors (P < 0.050). In multivariate logistic regression, the mean ADC value (SSF, 6) and kurtosis of CE-T1WI (SSF, 4) were independently associated with malignancy (P ≤ 0.009). A multivariate model of MR features worked well for diagnosis of malignant soft-tissue tumors (AUC, 0.909). Conclusion: Accurate diagnosis could be obtained using MR textural analysis with DWI and CE-T1WI in differentiating benign from malignant soft-tissue tumors.

• Journal of Korean Foot and Ankle Society
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• v.26 no.2
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• pp.78-83
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• 2022

Purpose: The purpose of this study was to evaluate the surgical outcome of split-thickness skin graft (STSG) for chronic diabetic wounds of the foot and ankle. Materials and Methods: The medical records of 20 patients who underwent surgery for chronic diabetic wounds of the foot and ankle between October 2013 and May 2018 were reviewed. Surgical management consisted of consecutive debridement, followed by negative-pressure wound therapy and STSG. We used an acellular dermal matrix between the wound and the overlying STSG in some patients with wide or uneven wounds. Patient satisfaction, comorbidities, wound size and location, length of hospital stay, wound healing time, and complications were investigated. Results: Of 20 patients, 17 (85.0%) were satisfied with the surgical outcome. Eight patients had diabetic wounds associated with peripheral vascular disease (PVD), 7 patients had diabetic wounds without PVD, and 5 patients had acute infection superimposed with necrotizing abscesses. The mean size of the wound was 49.6 cm². The mean length of hospital stay was 33.3 days. The mean time to wound healing was 7.9 weeks. The mean follow-up period was 25.9 months. Complications included delayed wound healing (4 cases) and recurrence of the diabetic wounds (2 cases), which were resolved by meticulous wound dressing. Conclusion: STSG remains a good treatment strategy for chronic diabetic wounds of the foot and ankle.

• Korean Journal of Pharmacognosy
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• v.54 no.1
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• pp.44-51
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• 2023

Fine dust has emerged as seriously hazardrous particles, which can cause respiratory and cardiovascular diseases. Thus, we have developed a natural complex extract consisted of Codonopsis lanceolata (Siebold & Zucc.) Trautv., Platycodon grandiflorum A. De Candolle, and Glycyrrhiza uralensis Fischer in the ratio of 3 : 5 : 2, which have a beneficial effect on respiratory system. In this study, simultaneous quantitative analysis of marker compounds, lobetyolin, platycodin D, and glycyrrhizic acid in the natural complex extract was developed and validated with high performance liquid chromatography-photodiode array detector. The marker compounds were shown in a large linearity with a correlation coefficient (R²) of 1.000. The limit of detection (LOD) of lobetyolin, platycodin D and glycyrrhizic acid were 5.40 ㎍/mL, 3.94 ㎍/mL and 5.86 ㎍/mL, respectively. The limit of quantification (LOQ) of lobetyolin, platycodin D and glycyrrhizic acid were 16.36 ㎍/mL, 11.94 ㎍/mL and 17.75 ㎍/mL, respectively. The content analysis revealed that the amounts of lobetyolin, platycodin D, and glycyrrhizic acid were 0.039±0.013 mg/g, 0.337±0.048 mg/g, and 1.171±0.003 mg/g, respectively, in the natural complex extract. Therefore, these results could be used as basic data for the standardization and quality control of the natural complex extract.

• Clinical Nutrition Research
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• v.12 no.3
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• pp.184-198
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• 2023

Early prevention of sarcopenia can be an important strategy for muscle maintenance, but most studies target subjects at slightly pre-sarcopenic state. Our previous paper describes the effect of protein supplements rich in leucine and vitamin D on muscle condition, and in this paper, we performed a sub-analysis to evaluate who benefitted the most in terms of improvement in muscle health. A 12-week randomized clinical trial of 120 healthy adults (aged 50 to 80) assigned to an intervention group (n = 60) or control group (n = 60) were analyzed. Subjects in the intervention group received, twice per day, a protein supplement containing (per serving) 800 IU of vitamin D, 20 g of protein (3 g of total leucine), 300 mg of calcium, 1.1 g of fat, and 2.5 g of carbohydrate. The subjects were classified into 'insufficient' and 'sufficient' groups at 25-hydroxyvitamin D (25[OH]D) value of 30 ng/mL. The skeletal muscle mass index normalized to the square of the skeletal muscle mass (SMM) height (kg/m²) increased significantly in the 'insufficient group' difference value of change between weeks 0 and 12 (Δ1.07 ± 2.20; p = 0.037). The SMM normalized by body weight (kg/kg, %) was higher, but not significantly, in the insufficient group (Δ0.38 ± 0.69; p = 0.050). For people with insufficient (serum 25[OH]D), supplemental intake of protein and vitamin D, calcium, and leucine and adequate energy intake increases muscle mass in middle-aged and older adults and would be likely to exert a beneficial effect on muscle health.

• The Korean Journal of Mycology
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• v.52 no.1
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• pp.55-60
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• 2024

In August 2023, leaf spot disease was observed in peanuts in Cheongju-si, Korea. Leaf spots occurred at the leaf margins and the lesions gradually expanded. Diseased leaf areas were light or dark brown and irregular in shape. A fungal isolate was obtained from symptomatic leaf and cultured on potato dextrose agar (PDA) medium at 25℃. An isolate was identified as Colletotrichum sojae based on morphological characteristics and sequences of the internal transcribed spacers, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase-1, actin, and 𝛽-tubulin genes. Pathogenicity tests were performed on peanut seedlings in a conidial suspension (1×10⁶ conidia/mL). Lesions were observed on the peanut leaf 5 d after inoculation. The pathogen was re-isolated from the lesions of the inoculated leaves. To the best of our knowledge, this is the first report of anthracnose on peanut caused by C. sojae in Korea.

• Journal of The Korean Astronomical Society
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• v.49 no.5
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• pp.199-208
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• 2016

We present photometric results of the δ Sct star V1162 Ori, which is extensively monitored for a total of 49 nights from mid-December 2014 to early-March 2015. The observations are made with three KMTNet (Korea Microlensing Telescope Network) 1.6 m telescopes installed in Chile, South Africa, and Australia. Multiple frequency analysis is applied to the data and resulted in clear detection of seven frequencies without an alias problem: five known frequencies and two new ones with small amplitudes of 1.2-1.7 mmag. The amplitudes of all but one frequency are significantly different from previous results, confirming the existence of long-term amplitude changes. We examine the variations in pulsation timings of V1162 Ori for about 30 years by using the times of maximum light obtained from our data and collected from the literatures. The O − C (Observed minus Calculated) timing diagram shows a combination of a downward parabolic variation with a period decreasing rate of (1/P)dP/dt = −4.22 × 10⁻⁶ year⁻¹ and a cyclic change with a period of about 2780 days. The most probable explanation for this cyclic variation is the light-travel-time effect caused by an unknown binary companion, which has a minimum mass of 0.69 M_⊙. V1162 Ori is the first δ Sct-type pulsating star of which the observed fast period decrease can be interpreted as an evolutionary effect of a pre-main sequence star, considering its membership of the Orion OB 1c association.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.6
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• pp.79-85
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• 2023

Skid resistance pavement is an accessory to the road and is a facility for the safe driving of cars by increasing the skid resistance of road pavement. In particular, in bad weather conditions such as snow, rain, and black ice, the skid resistance performance of skid resistance pavement greatly affects the safety of road traffic and drivers. However, BPT(British Pendulum Tester) has a test area of only 0.009 m², making it difficult to represent the overall packaging surface. A reliable method of evaluating slip resistance performance is needed for maintaining non-slip packaging. In this study, the conventional BPT test and the skid resistance performance evaluation method of the PFT(Pavement Friction Tester) and µGT(Micro Grip Tester) tests were compared through guidelines and standard investigations and applied to the field skid resistance performance evaluation. In addition, skid resistance pavement with different skid resistance performance was installed at the test-bed and actual road demonstration sites to compare BPN(British Pendulum Number), SN(Skid Number), GN(Grip Number), and to derive correlations for each performance evaluation method. As a result of the experiment, SN and GN showed similar skid resistance performance, and the GN value was derived similar to BPN × 0.01.

• Korean Journal of Head & Neck Oncology
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• v.23 no.1
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• pp.46-49
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• 2007

Soft tissue implantation of thyroid tissue is a very rare event. Needle tract implantation of thyroid carcinoma after fine-needle aspiration (FNA) biopsy has been occasionally reported, but implantation of benign thyroid tumor tissue is extremely rare. Rupture of thyroid tissues during surgery or trauma may cause the thyroid tissue to be implanted and result in multiple palpable nodules in soft tissue of the neck. Several reports have shown the possibility of implantation of normal or hyperplastic thyroid tissues in soft tissue. We herein report a case of implantation of adenomatous hyperplastic tissue in the neck along the trochar and previous operation site after endoscopic thyroid surgery, which was successfully treated by complete excision.

• Journal of the Korea Furniture Society
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• v.21 no.4
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• pp.314-325
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• 2010

Shake and gnarl in the timber market are considered as a defect of reducing the quality of timber. However, this study expects that shake and gnarl are the optimum design tools to improve aesthetics of furniture if their unique form is properly used as formative expression factors of wooden furniture. This study is conducted to suggest methods in order for consumers and designers to consider shake and gnarl as new formative factors and apply them to wooden furniture designs. The study conducts a preference survey of general people by manufacturing furniture that has or doesn't have shake and gnarl, and measures the level of aesthetics after providing furniture design majors with furniture image that reprocesses shake and gnarl in a new way. According to the findings, shake and gnarl have latent aesthetic values, which verifies that various methods can be used to improve aesthetics of wooden furniture by means of a case study. By applying hidden authentic value of the verified shake and gnarl to furniture design, various improvement methods of aesthetics will have to be actively studied.