• Title/Summary/Keyword: Chung Guk Jang

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Purification and Characterization of Fibrinolytic Enzyme Excreted by Bacillus subtilis K-54 Isolated from Chung Guk Jang. (청국장에서 분리한 Bacillus subtilis K-54가 분비하는 혈전용해효소의 정제 및 특성)

  • 유천권;서원상;이철수;강상모
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.507-514
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    • 1998
  • The strain K-54, the best producer of fibrinolytic enzyme, was isolated from Korean traditional food Chung Guk Jang and identified as Bacillus subtilis. Fibrinolytic enzyme was purified and characterized, and its molecular weight was determined. The fibrinolytic enzyme activity was increased about 66.9 times via purification with recovery yield of 10.1%. The optimum pH and temperature of this enzyme were 11 and $65^{\circ}C$. The enzyme was stable within a pH range 8-12 and unstable at 9$0^{\circ}C$. The molecular weight was estimated to be 29,000 dalton in the form of monomer with no other subunit. The isoelectric point was calculated 8.67. N-terminal sequence was identified Ala-Gly-Ser-Val-Pro-Try-Gly-Ser. Km value of the enzyme for $\alpha$-casein was calculated to be 0.31 (3.1 mg/$m\ell$). The enzyme activity highly inhibited by PMSF at 1 nM.

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Dual Band Design of Aperture-Coupled Cavity-Fed Microstrip Antenna (이중 대역 개구면 결합 공진기 급전 마이크로스트립 안테나 설계)

  • Jang, Guk-Hyun;Nam, Kyung-Min;Lee, Jang-Hwan;Nam, Sang-Ho;Kim, Chul-Un;Kim, Jeong-Phill
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.44 no.3 s.357
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    • pp.26-32
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    • 2007
  • A simple but accurate equivalent circuit of an aperture-coupled cavity-fed microstrip patch antenna is developed. It consists of ideal transformers, admittance elements, and transmission lines, and the related circuit element values are computed by applying the reciprocity theorem and complex power concept with the spectral-domain immittance approach. After validating by the published design example, a dual-band antenna was designed with the help of a hybrid optimization method. For this purpose, the Genetic Algorithm is applied with the Nelder-Mead simplex method. The obtained good results show that this approach turned out to be a very efficient tool for the design of aperture-coupled cavity-fed microstrip patch antenna having various structural design parameters.

On Study the Safety Diagnosis Assessment of EMU's Carbody (전동차의 구조체 안전진단평가 연구)

  • Chung Joog-Duk;Pyun Jang-Sik;Kim Jeong-Guk;Kim Won-kyung;Hong Yong-Ki
    • Proceedings of the KSR Conference
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    • 2004.06a
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    • pp.322-327
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    • 2004
  • This paper describes the structural analysis result of accident EMU(Electric Multiple Units). Structural analysis of EMU was performed for the criteria of safety assessment. Structural analysis using commercial I-DEAS software provided important information on the stress distribution and load transfer mechanisms as well as the amount of damages during rolling stock crash. Tensile tests were performed on two different types of specimens in order to evaluate the strength changes before and after damages, obtained from plastic deformed area and nondeformed region of the accident EMU. The structural analysis and tensile test results have been used to provide the critical information for the criteria of safety assessment.

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Microstructure and mechanical properties of Nd:YAG Laser welded AZ31-H24 Magnesium alloy using AZ61 filler metal (AZ61 filler wire를 사용하여 Nd:YAG Laser 용접한 AZ31-H24합금의 미세조직과 기계적 특성)

  • Ryu, Chung-Seon;Lee, Mok-Yeong;Bang, Guk-Su;Jang, Ung-Seong
    • Proceedings of the KWS Conference
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    • 2007.11a
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    • pp.322-324
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    • 2007
  • Nd:YAG laser welding of AZ31B-H24 magnesium alloy was carried out using AZ61 filler wire(Mg-6wt%Al-1wt%Zn). Microstructure and mechanical properties of welded joint were examined by optical microscopy, scanning electronic microscopy(SEM), energy dispersive spectroscopy(EDS), electron probe micro analyzer(EPMA) and victors hardness, tensile test at the room and elevated temperature. Test results indicate that the specimens welded with AZ61 filler wire have better tensile strength, elongation and victors hardness at room temperature than those of welded without filler wire. However tensile strength are similar but elongation are quite different at elevated temperature.

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