• Title/Summary/Keyword: Chun-Kwang

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The culture conditions for mycelial growth and sclerotial formation of Polyporus umbellatus

  • Lee, Min Woong;Chang, Kwang Chun;Shin, Do Bin;Lee, Kyung Rim;Im, Kyung Hoan;Jin, Ga-Heon;Shin, Pyung Gyun;Xing, Yong Mei;Chen, Juan;Guo, Shun Xing;Lee, Tae Soo
    • Journal of Mushroom
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    • v.11 no.4
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    • pp.194-200
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    • 2013
  • Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicines in China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillaria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture between P. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were $20^{\circ}C$ and pH 4, while optimal conditions for mycelial growth of A. mellea were $25^{\circ}C$ and pH 6. The carbon sources for optimal mycelial growth of P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were also fructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract, while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. mellea were dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose and maltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeast extract for 60 days at $20^{\circ}C$ under dark condition.

A Study on the Fabrication of the Solar Cells using the Recycled Silicon Wafers (Recycled Si Wafer를 이용한 태양전지의 제작과 특성 연구)

  • Choi, Song-Ho;Jeong, Kwang-Jin;Koo, Kyoung-Wan;Cho, Tong-Yul;Chun, Hui-Gon
    • Journal of Sensor Science and Technology
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    • v.9 no.1
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    • pp.70-75
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    • 2000
  • The recycled single crystal silicon wafers have been fabricated into solar cells. It can be a solution for the high cost in materials for solar cells and recycling of materials. So, p-type (100) single crystal silicon wafers with high resistivity of $10-14\;{\Omega}cm$ and the thickness of $650\;{\mu}m$ were used for the fabrication of solar cells. Optimistic conditions of formation of back surface field, surface texturing and anti-reflection coating were studied for getting high efficiency. In addition, thickness variation of solar cell was also studied for increase of efficiency. As a result, the solar cell with efficiency of 10% with a curve fill factor of 0.53 was fabricated with the wafers which have the area of $4\;cm^2$ and thickness of $300\;{\mu}m$. According to above results, recycling possibility of wasted wafers to single crystal silicon solar cells was confirmed.

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Unlinked and Convertible Total Elbow Arthroplasty (비연결형 및 전환형 주관절 전치환술)

  • Moon, Jun-Gyu;Chun, Sung-Kwang
    • Clinics in Shoulder and Elbow
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    • v.16 no.2
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    • pp.163-169
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    • 2013
  • Designs of total elbow arthroplasty have been evolving with clinical experiences. Newer implants are expected to resolve current limitations and improve long term outcomes. This review article focuses on the basic knowledge of unlinked and convertible total elbow arthroplasty. There have been a variety of designs of unlinked total elbow prostheses. Some implants are still used in the market, while others are no longer commercially available. Modified and newer designs include more congruent contact surface, stemmed implant, and radiocapitellar arthroplasty. Two convertible elbow prostheses have been developed, and one implant is currently available in Korea. Conversion from an unlinked to a linked mode is performed by adding a linking cap. Unlinked total elbow arthroplasty, which restores native elbow kinematics, has a biomechanical rationale of lowering polyethylene wear and loosening of implants. It can be indicated in younger and higher demand patients, who have adequate bone stock and soft tissues. Convertible total elbow arthroplasty broadens implant selection and simplifies revision surgery. These newer prostheses possibly improve the long term outcomes and resolve disadvantages of linked prostheses in total elbow arthroplasty.

The Comparison of Water Budget and Nutrient Loading from Paddy Field According to the Irrigation Methods (관개방법에 따른 논에서의 수문 및 수질특성에 미치는 영향)

  • Jeon, Ji-Hong;Choi, Jin-Kyu;Yoon, Kwang-Sik;Yoon, Chun-Gyeong
    • Korean Journal of Ecology and Environment
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    • v.38 no.1 s.110
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    • pp.118-127
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    • 2005
  • The comparison of water balance and nutrient loading from paddy field with different irrigation management were carried out during 1999 ${\sim}$ 2002 at two different sites; one is irrigated with groundwater and the other is irrigated with surface water. For the surface water irrigated paddy field, irrigation was performed continuously during growing season. Whereas, initial irrigation with groundwater was applied during initial growing season, and the ponded water depth was maintained by natural precipitation since initial irrigation. The runoff frequency of groundwater irrigated paddy field was less than that of surface water irrigated paddy field. The nutrient concentration of ponded water was high by fertilization at early cultural periods, so reducing surface drainage during fertilization period can reduce nutrient loading from paddy fields. Amount of irrigation water to surface water irrigated paddy field was higher than to groundwater irrigated paddy field and evapotranspiration was similar because it is influenced by climate. Overall input in and output from paddy field irrigated with goundwater were less than that with surface water. This study indicate that efficient water management can reduce surface drainage outflow, save water, and protect water quality. It might be important BMPs for paddy field.

An Analytical Solution of Dynamic Responses for Seabed under Coexisting Fields of Flow and Partial Standing Wave with Arbitrary Reflection Ratio (흐름과 임의반사율을 갖는 부분중복파와의 공존장하에서 해저지반내 동적응답의 해석해)

  • Lee, Kwang-Ho;Kim, Dong-Wook;Kang, Gi-Chun;Kim, Do-Sam;Kim, Tae-Hyung;Na, Seung-Min
    • Journal of the Korean Geotechnical Society
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    • v.31 no.6
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    • pp.27-44
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    • 2015
  • An analytical solution of dynamic responses for seabed in finite and infinite thicknesses including shallow has been developed under flow and partial standing wave with arbitrary reflection ration coexisting field at a constant water depth condition. In the analytical solution, a field was simply transited to a coexisting field of progressive wave and flow when reflection ratio was 0 and to a coexisting field of fully standing wave and flow when reflection ratio was 1. Based on the Biot's consolidation theory, the seabed was assumed as a porous elastic media with the assumptions that pore fluid is compressible and Darcy law governs the flow. The developed analytical solution was compared with the existing results and was verified. Using the analytical solution the deformation, pore pressure, effective and shear stresses were examined under various given values of reflection ratio, flow velocity, incident wave's period and seabed thickness. From this study, it was confirmed that the dynamic response of seabed was quite different depending on consideration of flow, which causes changing period and length of incident and reflection waves. It was also confirmed that dynamic response significantly depends on the magnitude of reflection ratio.

Isolation of Serratia marcescens CK-3 against phytopathogenic fungi and its enzymatic properties (식물(植物) 병원류(病源惟) 사상균(絲狀菌)에 길항력(拮抗力)을 갖는 Serratia marcescens CK-3의 분리(分離) 및 효소적(酵素的) 성질(性質))

  • Kim, Yeong-Yil;Rhee, Young-Hwan;Kim, Kwang-Sik;Park, Hwa-Sung;Chun, Woo-Bock;Lee, Jae-Wha;Kim, Jong-Hyun
    • Applied Biological Chemistry
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    • v.34 no.1
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    • pp.54-60
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    • 1991
  • Serratia marcescens CK-3, decomposing chitin which is a mar component of cell wall in phyitopathogenic fungi, was isolated from the continuous cropping rhizosphere of pepper and cucumber and its enzymatic property was examined. S. marcescens CK-3 was found tn have an tagonistic effects against, Fusarium axysporum and Rhizoctonia solani and to have complex enzyme system such as chitinase, laminarinase, and proteinase. The preferable composition of the medium for production of chitinase was fond and was as follows : colloidal chitin 1.5%, tryptone 0.5%, glucose 1.0%, peptone 0.2%, $MgSO_4{\cdot}7H_2O\;0.1%,\;K_2HPO_4\;0.1%,\;and\;NaCl\;0.1%$(w/v), pH 6.8. The maximum enzyme production was observed after culture of 72 hours at $30^{\circ}C$ using a medium containing the above chemical composition. The optimal pH and temperature for in vitro activity of chitinase from S. marcescens CK-3 were pH 7.5 and $50^{\circ}C$, respectively. The enzyme activity in-creased by metal ions such as$Ag^+$ and $Mn^{++}$.

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Gas Chromatographic Analysis on Residual Concentration and Half-life Time of Cyfluthrin in Potato and Soil (감자와 토양 중에서 Cyfluthrin의 잔류농도와 반감기에 대한 기체 크로마토그래피 분석)

  • Han, Seong Soo;Jeong, Seung Il;Chun, Hyun Ja;Hoang, Geun Chang;Kim, Il Kwang
    • Analytical Science and Technology
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    • v.13 no.6
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    • pp.722-730
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    • 2000
  • The residual concentration and half-life time of synthetic pyrethroid insecticide cyfluthrin in potato and soil were investigated by gas chromatography (GC). The pesticide in potato was extracted with n-hexane, filtered and concentrated. The concentrated phase was transferred to the Sep-Pak silica gel column and purified with acetonitrile and acetone for the analysis by gas chromatography equipped with electron capture detector (GC-ECD). From the standard additional experiments with 0.01 and $0.1{\mu}g$, the recoveries were 85-87% and the detection limit was 0.005 ng. The soil sample was extracted with acetone and dichloromethane. The organic phase was concentrated and redissolved with n-hexane and analyzed with GC-ECD after cleaned with Sep-Pak column. From the standard additional experiments with 0.01 and 0.1 ng, the recoveries were 84-88% and the detection limit was 0.005ng. The half-life time of cyfluthrin in the silty clay was 25 days in the room laboratory and 0.6 days in the fieJd test whereas it was 38 days and 0.5 days for each in case of silty loam.

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Combined utilization with herbal products and prescribed drugs: A result from health examinee-based national survey (일부 종합병원의 건강검진 수검자들의 한약·양약 복합투여)

  • Park, Jong-Ku;Choi, Seo-Young;Koh, Kwang-Wook;Yu, Jun-Sang;Kim, Tae-Hun;Sohn, Dong-Kook;Hong, Ju-Hee;Song, Sung-Eun;Kim, Chun-Bae
    • Health Policy and Management
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    • v.16 no.1
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    • pp.1-16
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    • 2006
  • Recently, there has been prevailing of the combined utilization (CU) with herbs and prescribed drugs in medical therapies in the world. But the information about frequency, efficacy and safety of this CU has not well known in Korea, yet. This study aimed to identify the status of CU by Koreans, and to inquire which side effects of CU represent to those people. A self-completed questionnaire survey was performed through each health examination center in twenty general hospitals and one oriental hospital. Of the initial 2,100 health examinees, 1,851 were participated in this survey, resulting in a response rate of 88.1%. The proportion of CD was 26.3%. The most commonly mentioned reason of CD was 'to promote general health and well-being' (17l persons, 35.5%). The main route of taking CD was self-purchase at drugstore or at herbs market, followed by the prescription of (oriental)physicians. 33.0% (151 persons) of those who took the CD rated it as effective. 93 respondents (19.8%) were experienced several adverse effects including nausea, fatigue, and dizziness. The growing simultaneous use of herbal products and pharmaceutical drugs by Korean consumers may be continuously increased the risk of herb-drug interactions. The medical professionals should be provided with comprehensive and up-to-date information about potential benefits and risks of herbs and prescribed drugs. In the future studies it may be recommended to deal common cold, health promotion, indigestion, headache, and hypertension for the effect and safety of the CD by well-organized prospective study design.

Cordycepin Induced Apoptosis via Intracellular Ca2+ Modulation and Mitochondrial Dysfunction in Human Prostate Cancer PC-3 Cells (전립선암 세포주인 PC-3에서 cordycepin에 의해 유도된 세포 내 칼슘농도 변화와 미토콘드리아 기능 상실을 통한 세포사멸 유도)

  • Kang, Dong-Min;Kim, Kwang-Youn;Yu, Sun-Nyoung;Jin, Young-Rang;Jeon, Hyun-Joo;Kim, Sang-Hun;Chun, Sung-Sik;Ko, Hack-Ryong;Ahn, Soon-Cheol
    • Journal of Life Science
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    • v.21 no.3
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    • pp.451-458
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    • 2011
  • Cordycepin (3'-deoxyadenosine), a nucleoside derivative isolated from Cordyceps militaris, is reported to have antitumor effects. However, neither its molecular mechanism nor its molecular targets are well understood. In the present study, molecular mechanisms for the anti-tumor effects of cordycepin were investigated in human prostate cancer PC-3 cells. The MTT assay was used to detect cell viability. Annexin V/FITC assay, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), and $Ca^{2+}$ flux were used to assess for the presence of apoptosis. Western blot analysis was used to detect protein expression. Treatment of cordycepin resulted in significantly decreased cell viability of PC-3 cells in a dose- and time-dependent manner. A dose-dependent apoptotic cell death was also measured by flow cytometery analysis. Molecular mechanistic studies of apoptosis unraveled cordycepin treatment resulted in significant mitochondrial dysfunction, ROS production, and elevation of $Ca^{2+}$ concentrations. These phenomena were followed activation of caspase-3, subsequently leading to PARP cleavage and cell apoptosis. Taken together, cordycepin induces apoptosis in PC-3 cells through regulation of a mitochondrial mediated pathway.

Effects of Convection Oven Dehydration Conditions on the Physicochemical and Sensory Properties of Ginkgo Nut Powder (열풍건조 조건에 따른 은행분말의 이화학적 및 관능적 특성)

  • Kim, Jung-Mi;Lee, Young-Chun;Kim, Kwang-Ok
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.393-398
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    • 2003
  • Effects of convection oven dehydration conditions on the physicochemical and sensory properties of ginkgo nut powder were examined using three types of pre-treatment on ginkgo nuts: coarse grinding of ginkgo nut (GR); coarse grinding followed by 1 min blanching (GB); 3 min blanching followed by coarse grinding (BG). Pretreated ginkgo nuts were dried in convection oven at 70 and $80^{\circ}C$ to the moisture content of approximately 5%. Rehydration rate, swelling power, solubility, lightness, and greenness of GB ginkgo nut powder dried at $70^{\circ}C$ were the most similar to those of freeze dried one. GR and GB samples dried at $70^{\circ}C$ had higher sensory values of green color and ginkgo nut flavor. Ginkgo nut powder with desirable quality attributes could be produced by drying GB in convection oven at $70^{\circ}C$ for 10 hr.