• 한국균학회지
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• 제22권3호
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• pp.254-259
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• 1994

P. chrysosporium BKM-F-1767 및 C. subvermispora FP90031-SP. 균사를 이용하여 ligninase와 laccase 활성제고를 위한 배앙방법을 검토한 결과는 다음과 같다. P. chrysosporium의 왕복진탕배양시 (150 rpm) 배양액량은 45-60 ml가 ligninase 활성제고에 적합하였으며 60 ml 배양은 최대활성 출현일이 빨리 나타났다. C. subvermispora의 정치배양시, 2% M.E.+0.1% Y.E.+0.1% T.W.20+1mMVA 처리에서 ligninase 활성이 가장 높았고 laccase의 활성은 2% M.E.+0.1% Y.E.+0.1% T.W.20+6 mMBA에서 최고 수준을 나타내었다.

• Journal of the Korean Wood Science and Technology
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• 제45권2호
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• pp.168-181
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• 2017

Whole cell of Phanerochaete chrysosporium with reducing agent was applied to verify the degradation mechanism of aromatic compounds derived from lignin precisely. Unlike the free-reducing agent experiment, various degraded products of aromatic compounds were detected under the fungal treatment. Our results suggested that demethoxylation, $C_{\alpha}$ oxidation and ring cleavage of aromatic compounds occurred under the catabolic system of P. chrysosporium. After that, degraded products stimulated the primary metabolism of fungus, so succinic acid was ultimately main degradation product of lignin derived-aromatic compounds. Especially, hydroquinone was detected as final intermediate in the degradation of aromatics and production of succinic acid. In conclusions, P. chrysosporium has an unique catabolic metabolism related to the production of succinic acid from lignin derived-aromatic compounds, which was meaningful in terms of lignin valorization.

• Journal of Forest and Environmental Science
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• 제6권1호
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• pp.45-60
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• 1989

리그닌 생분해의 분해경로 및 매카니즘에 관한 연구가 최근 Kirk와 Higuchi 등에 의하여 활발히 연구되고 있다. 특히, Phanerochaete chrysosporium이 생산해내는 Lignlnase를 이용하여 매우 가치있는 연구 결과를 얻고 있다. 본 총설에서는 Kirk와 Higuchi의 허가를 얻어서 그들의 논문을 중심으로 리그닌의 중요한 결합 양식 별로 즉, ${\beta}$-O-4, ${\beta}$-5, ${\beta}$-1, ${\beta}$-6, 5-5 등의 결합 모델 화합물들의 분해경로 및 매카니즘에 관하여 조사 정리하였다.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2005년도 춘계학술발표논문집
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• pp.301-302
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• 2005

PAHs의 분해를 위해 Bacillus subtilis 와 Phanerochaete chrysosporium의 두 미생물을 사용하였고, 이들을 부착시킬 담체로 코르크와 톱밥를 선정하여 미생물을 접종시켰다. Phenanthrene 분해의 경우, 반응 초기에는 분해 속도가 매우 빠르지만 반응 12일째 분해 속도가 떨어졌다. 분해 속도는 1)톱밥에 담지한 Bacillus subtilis, 2)코르크에 담지한 Bacillus subtilis, 3)톱밥에 담지한 Phanerochaete chrysosporium, 4)코르크에 담지한 Phanerochaete chrysosporium의 순으로 나타났다.

• KSBB Journal
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• 제15권3호
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• pp.247-253
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• 2000

This study was aimed to enhance polycyclic aromatic hydrocarbon(PAHS) biodegradation rate by repeated-batch treatment using immobilized cells of Phanerochaete chrysosporium. In the repeated-batch operations with 30 mg/L of pyrene the maximum degradation rate was 6.58 mg/L day. As the number of batches increased the concentration of immobilized cells significantly decreased and the degradation rate and specific acitivity gradually increased to a maximum value and then decreased. To have PAH degradation activity and cell mass recovered one batch of cultivation using the growth medium instead of the PAH-degrading medium was carried in the course of repeated-batch operations. This maximum degradation rates of pyrene and anthracene were 4.29 and 4.46 mg/L$.$day respectively. Overall the rate of PAH degradation could be enhanced 2.5-30 folds by using immobilized cells compared to the case of using suspended cells.

• 한국염색가공학회지
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• 제12권1호
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• pp.32-43
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• 2000

Batch culture system and continuous culture systems were used to investigate the removal of disperse dye using white rot fungi. White rot fungi used in the study were Coriolus hirsutus IFO 4917, Lenzites betulina IFO 6266, Coriolus versicolor IFO 30340 and Phanerochaete chrysosporium IFO 31249. The results of the batch culture experiment showed that white rot fungi used in this study had excellent dye removal abilities. Phnerochete chrysosporium IFO 31249 was especially effective on the removal of disperse dyes. And continuous treatment of disperse red 60 was studied under two type of reactor using Phanerochaete chrysosporium IFO 31249. The removal efficiency of disperse red 60 for immobilized Phanerochaete chrysosporium IFO 31249 in continuous reactor with vertical matrix was increased 1.3 fold in $1.4\;hr^{-1}$ dilution rate when compared with continuous reactor without vertical matrix.

• 한국균학회지
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• 제51권2호
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• pp.91-100
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• 2023

During an investigation of micro-fungi in soil, two fungal isolates belonging to the phylum Ascomycota, namely KNUF-20-NI011 and KNUF-20-NI006, were collected from Gyeongbuk Province and Dokdo Island in Korea and identified as Clonostachys divergens and Chrysosporium merdarium, respectively. The fungal isolates were confirmed through molecular phylogenetic analyses of the internal transcribed spacer regions, 28S rDNA large subunit, and β-tubulin sequences. Cultural and morphological characteristics were observed and determined using different media. These species were identified based on phylogenetic relationships along with their cultural and morphological characteristics. To our knowledge, this is the first report on Clonostachys divergens and Chrysosporium merdarium in Korea.

• 한국균학회지
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• 제21권4호
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• pp.310-315
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• 1993

P. chrysosporium, SC 26 균주(菌株)를 이용하여 0.1% Tween 20, veratryl alcohol 및 benzyl alcohol 이 첨가된 배지(培地)에 접종(接種)한 후 진탕배양하여 ligninase역가를 조사한 결과는 다음과 같다. 배지(培地)에 Tween 20를 단독으로 처리했을 경우에는 ligninase 의 생성(生成)이 적었으나, 0.1% Tween 과 0.4 mM veratryl alcohol을 첨가했을 때 높았으며 , 최대 역가까지의 소요일수는 5일이었다. 또한 0.1% Tween 20 과 10 mM benzyl alcohol을 처리했을때 ligninase의 역가가 가장 높았으며, 최대 역가까지의 소요일수는 8일이였다.

• Journal of Microbiology and Biotechnology
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• 제8권2호
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• pp.129-133
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• 1998

The white-rot fungi Phanerochaete chrysosporium ATCC 24725, Pleurotus ostreatus ATCC 32783, Lentinus edodes ATCC 24462, and Trametes versicolor ATCC 42530 were studied for their ability to degrade lignin, phenanthrene, and anthracene. Lignin in rice-straw was degraded by 14.4, 28.73, and 33.88% by P. chrysosporium, T. versicolor, and P. ostreatus, respectively. Approximately 12% and 83% of phenanthrene was degraded in 1 and 5 days, respectively, when the pre-grown mycelIium matrix of P. ostreatus. was incubated with 10 ppm of phenanthrene in modified Kirk's medium (nitrogen limited) at $25^{\circ}C$. Approximately 2%> and 61% of phenanthrene was degraded when the phenanthrene concentration was increased to 30 ppm. Similar trends were observed with phenanthrene using P. chrysosporium. Mycelial growth of T. versicolor was less inhibited at 30 ppm phenanthrene than for P. ostreatus and P. chrysosporium. Better degradation of phenanthrene by T. versicolor may be attributed to better mycelium growth. One hundred percent of 15 ppm anthracene was degraded in 10 days by both P. chrysosporium and T. versicolor. 40 ppm anthracene inhibited the mycelial growth of P. chrysosporium. lignin peroxidase activity, which was previously reported to be involved in initial phenanthrene oxidation, was also detected from the culture broth of the strains tested. The rates of lignin peroxidase production in the cultures were not consistent with the rate of PAH hydrolysis during incubation.

• Journal of Microbiology
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• 제44권2호
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• pp.177-184
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• 2006

The characteristic biodegradation of monomeric styrene by Phanerochaete chrysosporium KFRI 20742, Trametes versicolor KFRI 20251 and Daldinia concentrica KFRI 40-1 was carried out to examine the resistance, its degradation efficiency and metabolites analysis. The estrogenic reduction effect of styrene by the fungi was also evaluated. The mycelium growth of fungi differentiated depending on the concentration levels of styrene. Additionally P. chrysosporium KFRI 20742 showed superior mycelium growth at less than 200 mg/l, while D. concentrica KFRI 40-1 was more than 200 mg/l. The degradation efficiency reached 99 % during one day of incubation for all the fungi. Both manganese-dependent peroxidase and laccase activities in liquid medium were the highest at the initial stage of incubation, whereas the lowest was after the addition of styrene. However, both activities were gradually recovered after. The major metabolites of styrene by P. chrysosporium KFRI 20742 were 2-phenyl ethanol, benzoic acid, cyclohexadiene-1,4-dione, butanol and succinic acid. From one to seven days of incubating the fungi, the expression of pS2 mRNA widely known as an estrogen response gene was decreased down to the level of baseline after one day. Also, the estrogenic effect of styrene completely disappeared after treatment with supernatant of P. chrysosporium KFRI 20742 from one week of culture down to the levels of vehicle.