• Applied Biological Chemistry
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• v.40 no.1
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• pp.85-88
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• 1997

In orther to isolate antibacterial substances from the flower of Chrysanthemum zawadskii Herbich var. latilobum Kitam., the chloroform fraction was fractionated according to the activity against B. subtilis, S. aureus and V. parahaemolyticus. Two antibacterial substances were isolated and purified by preparative TLC and recrystallization. Compound I showed activity against all the tested bacteria and compound II exhibited the activity against B. subtilis and v. parahaemolyticus except S. aureus. Compared to benzoic acid and sorbic acid which are being used as food preservatives, compounds I and II showed about five-fold stronger antibacterial activity against B. subtilis and V. parahaemolyticus. On the basis of spectrometric studies including $^1H-NMR,\;^{13}C-NMR,\;DEPT,\;^1H-^1H\;COSY,\;^{13}C-^1H\;COSY$ and Mass, compounds I and II were identified as angeloylcumambrin B and cumambrin A, respectively. This is the first report of the isolation of angeloylcumambrin B and cumambrin A from this plant.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.2
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• pp.1-8
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• 2012

This study is carried out to find the most optimal soil-plant combination in the urban agriculture by analyzing the association of soil base material which is being used in the urban agriculture with the growth of plants. 4 types of easily purchased soil (bed soil(A), animal vermicast soil(B), earth worm soil(C) and matured compost(D)) verified in aspects of effect and safety of soil in terms of growth of crop is selected as experimental soil and B, C, D type soils are mixed with granite soil at the ratio of 7 : 3. And granite soil(E) is set as a controlled soil and is compared to verify the effect of the experimental mixed soil. Herbaceous plants are classified into the fruit vegetables (Lycopersicon esculentum Mill. and Capsicum annuum L.), leafy vegetables (Brassica campestris L. ssp. Pekinensis and Lactuca sativa L.), medicinal vegetables (Chrysanthemum zawadskii var. latilobum and Liriope platyphylla F. T. Wang & T. Tang). The results of comparison of growth of herbaceous plants in different soil types showed that fruit vegetables and leafy vegetables in general had excellent growth in D type soil mixture and A type soil in general and had the poorest growth in E type (controlled) soil. 'Chrysanthemum zawadskii var. latilobum' had the excellent growth in D type, B type, C type mixed soil and A type soil and E type (controlled) soil are followed in order. In the case of 'Liriope platyphylla F. T. Wang & T. Tang', the difference in growth by each soil was shown to be insignificant. Therefore, the soil applied in the urban agriculture varies depending on each species of herbaceous plants, but it is considered effective to cultivate herbaceous plant which is economical and productive by using D type mixed soil which can be recycled and inexpensive compared with other experimental soils in the urban agriculture.

• Archives of Pharmacal Research
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• v.25 no.2
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• pp.170-177
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• 2002

The herb, Chrysanthemum zawadskii var, latilobum commonly known as Gu-Jul-Cho in Korea, used in traditional medicine to treat pneumonia, bronchitis, cough, common cold, pharyngitis, bladder-related disorders, gastroenteric disorders, and hypertension. Linarin is the main active compound and the biological mechanisms of its activity are unclear. It is believed that effects of this herb may be exerted through the pluripotent effectors of linarin due to its ability to treat a variety of afflictions. In this study, the effects of linarin on the mouse macrophages cell line, RAW 264.7, were investigated. It was found that linarin could activate macrophages by producing cytokines. Monocytes and tissue macrophages produce at least two groups of protein mediators of inflammation, interleukin 1 (IL-1 ) and the tumor necrosis factor (TNF). Recent studies have shown that TNF and IL-1 modulate the inflammatory function of endothelial cells, leukocytes, and fibroblasts. $TNF-{\alpha}$ production by macrophages treated with linarin occured in a dose dependent manner However, IL-1 production was largely unaffected by this natural product. This study demonstrated the ability of linarin to activate macrophages both directly and indirectly. Linarin also affect both cytosine production and nitric oxide inhibition, in addition to the expression of some surface molecules. Nitric oxide (NO), derived from L-argin-ine, is produced by two forms(constitutive and inducible) of nitric oxide synthase (NOS). The NO produced in large amounts by inducible NOS is known to be responsible for the vasodilation and hypotension observed in septic shock. Linarin was found to inhibit NO production in the LPS-activated RAW 264.7 cells. Linarin may be a useful candidate as a new drug for treating endotoxemia and the inflammation accompanied by NO overproduction. The linarin-treated total Iymphocytes exhibited cytotoxicity in a dose dependent manner between $20{\;}{\mu}g/ml{\;}and{\;}40{\;}{\mu}g/ml$. These results suggest that linarin may function through macrophage activation.

• Horticultural Science & Technology
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• v.29 no.1
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• pp.68-73
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• 2011

To identify and quantify the major antioxidants present in Chrysanthemum zawadskii var. latilobum (C. zawadskii, hereafter), the flowers of C. zawadskii were cut into pieces, extracted with methanol (MeOH), and fractionated by using n-hexane, chloroform ($CHCl_3$), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water. The highest concentration of polyphenol and flavonoid was observed in the EtOAc fraction. Based on ABTS and DPPH methods, highest antioxidative activities of C. zawadskii were found in the EtOAc fraction, in their 81.56% and 68.12% levels, respectively. By using HPLC, we identified two flavonoids: quercetin and luteolin, in the EtOAc fraction, and their contents were 20.02 and 1.65 $mg{\cdot}g^{-1}$, respectively. Our results clearly suggest the presence of these antioxidants in the flower of C. zawadskii, thus it may be consumed as tea with health beneficial effects.

• YAKHAK HOEJI
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• v.47 no.4
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• pp.234-238
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• 2003

Linarin is a main compound from Chrysanthemum zawadskii var, latilobum. However, the biological mechanisms of these activities are unclear. Because of this wide diversity of effects, it is believed that they may be exerted through pluripotent effectors of linarin. In our previous screening study, the effects of linarin on the mouse macrophages cell line, RAW 264.7 cells, were investigated. It was found that linarin could stimulate macrophages activation by the production of tumor necrosis factor (TNF). The linarin (6.25∼12.5 $\mu\textrm{g}$/mι) inhibited the production of NO in LPS-activated RAW 264.7 cells and linarin became an useful candidates for the development of new drug to treat endotoxemia and inflammation accompanied by the overproduction of NO. However, linarin-treated total lymphocyte showed cytotoxicity in a dose dependent manner between 20 $\mu\textrm{g}$/mι and 40 $\mu\textrm{g}$/mι. In this study, linarin derivative (acetylated linarin) was synthesized in order to obtain less-cytotoxicity of linarin and evaluated for their in vitro cytotoxic activity aganist mouse total lymphocyte. There was no cytotoxic activity in a dose dependent manner (20∼40 $\mu\textrm{g}$/mι) of acetylated linarin whereas linarin showed. The production of NO, however, was not the case by this modified linarin. The cell morphological change was not significantly changed in response to acetylated linarin alone and these effects were potentiated by the addition of LPS. These results suggest that acetylated linarin may be developed to be a promising new drug candidate without cytotoxicity on the basis of its activity of macrophage activation.

• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.61-65
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• 2000

It was investigated 91 families, 305 genera, 470 species, 1 subspecies, 30 varieties, 2 forma and total 503 taxa of herbal resources plants in Chollabuk-do area in this study. Among them 45 taxa belong to Compositae, 30 taxa belong to Rosaceae and 23 taxa belong to Leguminosae in order were occurred frequently. In general the herbal resources plants were distributed abundantly in Togyusan, Naejangsan, Changansan and Taedunsan area. Comparatively high frequent species was surveyed as follows: Schizandra chinensis, Cimicifuga heracleifolia, C. simplex, Asarum sieboldii, Arisaema amurense var. serratum, Polygonatum odoratum var. pluriflorum, Prunella vulgaris var. lilacina and Veratrum patulum etc. There was growing 30-50 individuals of Codonopsis lanceolata in 5m$\times$5m quadrat, 90 Chrysanthemum zawadskii var. latilobum plant in 1m$\times$1m and 100 Polygonatum odoratum var. pluriflorum plant in 1m$\times$1m quadrat from Togyusan quantitatively. Rare and herbal worth species were observed to distribute such as Gastrodia elata in Naejangsan and Togyusan and Acanthopanax senticosus in Mandoksan, Chinan-gun, respectively. Cultivating species in the farm now and/or favorable species in the future will be recommended here, for example, Codonopsis pilosus, A. senticosus, G. elata, Rubus coreanus, C. lanceolata and Pleuropterus multiflorus.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.220-223
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• 2011

Human basophilic KU812F cells express a high affinity for IgE receptor, $Fc{\varepsilon}RI$, which plays an important role in immunoglobulin E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of the ethanolic extract from Chrysanthemum zawadsaki(CZ) on $Fc{\varepsilon}RI$ ${\alpha}$ chain expression. Flow cytometric analysis showed that cell surface $Fc{\varepsilon}RI$ ${\alpha}$ chain expression was suppressed when the cells were cultured with CZ extract. RT-PCR analysis showed that the mRNAs for $Fc{\varepsilon}RI$ ${\alpha}$ chain decreased by the treatment of CZ extract. Moreover, CZ extract was also observed to result in a reduction in the release of histamine from anti-$Fc{\varepsilon}RI$ antibody (CRA-1)-stimulated cells. These results suggest that CZ extract may exert anti-allergic activity through down-regulation of $Fc{\varepsilon}RI$ ${\alpha}$ chain expression and a subsequent decrease in histamine release.

• Journal of Oriental Neuropsychiatry
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• v.19 no.2
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• pp.209-221
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• 2008

목적 : 구절초는 국화과에 속하는 다년생 초본으로 가을에 줄기와 잎을 가을에 채취한 것을 한약재로 사용하여 대한, 월경불순 등 각종 여성질환과 함께 위냉증, 소화불량, 감고, 폐렴, 기관지염, 배뇨장애 및 신경퇴행성 질환에 활용되고 있다. 본 연구에서는 LPS로 염증유도된 대식세 포주를 활용하여 구절초의 항염증효능을 확인하고자 하였다. 방법 : 구절초 추출물의 항염증효과를 관찰하기 위하여 RAW264.7 대식세포주에서 MTT cytotoxic assay, iNOS 및 COX-2 발현, NO와 PGE2 생성 및 $NF-{\kappa}B$ 활성실험을 수행하였다. 결과 : 세포독성실험을 통하여 구절초 추출물의 안전성이 확인되었으며 LPS로 염증유도된 RAW264.7 대식세포주에서 증가된 iNOS의 발현이 감소되었음을 확인하였다. 또한 NO 및 PGE2의 생성을 억제하였다. 이러한 결과는 구절초가 염증매개물질의 생성을 억제하는 효과가 있음을 확인할 수 있었으며, $NF-{\kappa}B$ 활성도를 감소시킴을 관찰하였다. 결론 : 이러한 결과는 구절초가 $NF-{\kappa}B$ pathway를 조절해 줌으로써 항염증효과를 나타내는 것으로 사료된다.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.4
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• pp.67-74
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• 2008

This study was carried out to select proper species for early stage replantation in cutting-slope area of the Baekdu Range roadside. In the cutting-slope area and upper forest area of the cutting-slope area, sample plots of 104 were selected and their vegetations and environmental factors were investigated. We found total 151 species in the 104 plots. The species of high frequency of Baekdu Range roadside were found in the order of Lespedeza bicolor, Festuca arundinacea, Eragrostis curvula, Pinus densiflora, Salix hulteni, Lespedeza cuneata, Erigeron annuus, Rubus crataegifolius, Spodiopogon sibiricus, etc. The dominancy of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Pinus densiflora, Salix hulteni, Spodiopogon sibiricus, Miscanthus sinensis, Lespedeza maximowiczii, Acer ginnala were 12.74%, 10.43%, 7.48%, 4.42%, 4.09%, 3.13%, 2.49%, 2.45%, 2.40% in the roadside cutting-slope, respectively. The importance value of Quercus mongolica, Lindera obtusiloba, Rhododendron mucronulatum, Lespedeza maximowiczii, Rhododendron schlippenbachii, Lespedeza bicolor, Quercus serrata, Rhus trichocarpa were 17.32%, 13.82%, 11.60%, 11.31%, 8.76%, 8.04%, 7.65%, 7.10% in shrub layer of the upper forest area, respectively. The species of Carex lanceolata, Spodiopogon sibiricus, Carex siderosticta, Miscanthus sinensis, Artemisia sp., Potentilla fragarioides var. major, Astilbe chinensis var. davidii, Chrysanthemum zawadskii var. latilobum, Aster scaber, Lysimachia clethroides, Patrinia villosa, Lindera obtusiloba, Rhododendron mucronulatum, Rhododendron schlippenbachii, Rhus trichocarpa, Stephanandra incisa, Zanthoxylum schinifolium, Lespedeza sp., Lespedeza cuneata, Amorpha fruticosa, Ligustrum obtusifolium, and Weigela subsessilis, may be more helpful in stabilizing of the cutting-slope area and making harmony with the surrounding forest area.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.74-78
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• 1999

Nine sesquiterpene lactones, which were isolated from Hemisteptia lyrata Bunge, Chrysanthemum zawadskii Herbich var. latilobum Kitamura and Chrysanthemum boreale Makino were evaluated for the inhibition upon the nitric oxide release from the Raw cell and for the ACAT (acyl Co A: cholesterol acyltransferase) inhibitory assay. In the nitric oxide release inhibitory experiment, hemistepsin B $(IC_{50}\;=\;0.05\;{\mu}g/ml,\;0.15\;{\mu}M),$ cumambrin B, tulipinolide and costunolide exhibited strong inhibition with $IC_{50}$ values $0.05\;{\mu}g/ml\;(0.15\;{\mu}M),\;0.1\;{\mu}g/ml\;(0.38\;{\mu}M),\;0.2\;{\mu}g/ml\;(0.69\;{\mu}M)$ and $0.2\;{\mu}g/ml(0.86\;{\mu}M),$ respectively. In the ACAT inhibitory assay, angeloylcumambrin B, tigloylcumambrin B, tulipinolide and costunolide exhibited strong inhibition with $IC_{50}$ values $33\;{\mu}g/ml\;(95.4\;{\mu}M),\;22\;{\mu}g/ml\;(63.6\;{\mu}M),\;38\;{\mu}g/ml\;(151\;{\mu}M)$ and $17\;{\mu}g/ml(73.3\;{\mu}M),$ respectively, whereas other compounds did not exhibit significant activity against ACAT above $100\; {\mu}g/ml.$.