• Journal of Microbiology
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• v.43 no.4
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• pp.361-365
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• 2005

In this study, we chronicle the establishment of a novel transformation system for the unicellular marine green alga, Dunaliella salina. We introduced the CaMV35S promoter-GUS construct into D. salina with a PDS1000/He micro-particle bombardment system. Forty eight h after transformation, via histochemical staining, we observed the transient expression of GUS in D. salina cells which had been bombarded under rupture-disc pressures of 450 psi and 900 psi. We observed no GUS activity in either the negative or the blank controls. Our findings indicated that the micro-particle bombardment method constituted a feasible approach to the genetic transformation of D. salina. We also conducted tests of the cells' sensitivity to seven antibiotics and one herbicide, and our results suggested that 20 ${\mu}g$/ ml of Basta could inhibit cell growth completely. The bar gene, which encodes for phosphinothricin acetyltransferase and confers herbicide tolerance, was introduced into the cells via the above established method. The results of PCR and PCR-Southern blot analyses indicated that the gene was successfully integrated into the genome of the transformants.

• International Journal of Knowledge Content Development & Technology
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• v.9 no.3
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• pp.23-41
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• 2019

This study combines the concept of degree centrality in complex network with the Term Frequency $^*$ Proportional Document Frequency ($TF^*PDF$) algorithm; the combined method, called PMCN (PDF-Modified similarity and Complex Network), constructs relationship networks among sentences for writing news summaries. The PMCN method is a multi-document summarization extension of the ideas of Bun and Ishizuka (2002), who first published the $TF^*PDF$ algorithm for detecting hot topics. In their $TF^*PDF$ algorithm, Bun and Ishizuka defined the publisher of a news item as its channel. If the PDF weight of a term is higher than the weights of other terms, then the term is hotter than the other terms. However, this study attempts to develop summaries for news items. Because the $TF^*PDF$ algorithm summarizes daily news, PMCN replaces the concept of "channel" with "the date of the news event", and uses the resulting chronicle ordering for a multi-document summarization algorithm, of which the F-measure scores were 0.042 and 0.051 higher than LexRank for the famous d30001t and d30003t tasks, respectively.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.903-920
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• 2020

Since its first demonstration as a practical genome editing tool in the early 2010s, the use of clustered regularly interspaced short palindromic repeat (CRISPR) along with the endonuclease Cas9 (CRISPR/Cas9) has become an essential choice for generating targeted mutations. Due to its relative simplicity and cost-effectiveness compared to other molecular scissors, i.e., zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN), the CRISPR/Cas9 system has been shown to have a massive influence on genetic studies regardless of the biological kingdom. Although the system is in the process of being established, numerous protocols have already been released for the system and there have been various topics of CRISPR related papers published each year in ever-increasing manner. Here, we will briefly introduce CRISPR/Cas9 system and discuss the variants of the CRISPR system. Also, their applications to crop improvement will be dealt with mainly ornamental crops among horticultural crops other than Arabidopsis as a model plant. Finally, some issues on the barriers restraining the use of CRISPR system on floricultural crops, the prospect of CRISPR system as a DNA-free genome editing tool with efficient facilitators and finally, the future perspectives on the CRISPR system will be described.

• The Journal of Asian Finance, Economics and Business
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• v.9 no.2
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• pp.15-27
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• 2022

The paper addresses the importance of redesigning strategies for resilient and sustainable regional economic development - by applying strategic approaches to free economic zones for the digital economy. The purpose of the paper is to provide a broad comparative perspective on developed versus developing country efforts to use free economic zone programs to enhance various economic and social objectives. First, the paper introduces the chronicle experiences of free economic zone programs in South Korea and mainland China - developed versus developing economies. The main results of the analysis indicate that (1) achieving the goals and objectives of free economic zones is challenging over time; (2) trying to upgrade the technical component, develop knowledge-based and eco-friendly new industries, or value-added to the economy through free economic zone policies is even challenging; and (3) incentives and other program-specific variables are highly context-specific and not structurally correlated with free economic zone performance. In addition, the paper shows that the initial conception of the role of free economic zones has undergone significant change as individual countries have tried to enhance the contributions and avoid damages from incorporating free economic zones into their development strategy respectively, over time.

• Iberoamérica
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• v.23 no.2
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• pp.179-208
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• 2021

Luis Humberto Crosthwaite, as a witness to the accelerated changes in Tijuana since the 80's, has built a chronicle of the city based on nostalgia, fantasy, popular language, music, and criticism of the inequality between both sides of the border, but above all, on humour and irony. Among the gallery of characters that populate his stories, the common resident of the border has a special place. Here we are not talking about the passing person or newcomers, but of those who have shaped their social and personal identity from a long every day relationship with the city and the borderline, which makes up what we will call the border-being. In this work, we dialogue with the psychoanalytic concept of border personality or borderline disorder, which refers in a general way to subjects with a deep fracture between the self and the being, which prompts a psychotic search to reconcile this division. In addition, we will engage in an interdisciplinary dialogue to analyse how Crosthwaite characterises the fracture of the border-subjects in some of the stories of the book Instrucciones para cruzar la frontera, to point out the psychosis caused by the sociocultural tensions of life in a border city like Tijuana.

• The Journal of the Korea Contents Association
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• v.8 no.12
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• pp.214-221
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• 2008

Terrestrial magnetism has left traces through residues such as fossils of the terrestrial magnetism as time went by. An analysis of archaeological terrestrial magnetism is an estimation of dates of archaeological remains where baked earth is exposed by measuring the change of the past terrestrial magnetism through thermo-remnant magnetization of baked earth. This paper attempts to apply an analysis of the archaeological terrestrial magnetism to archaeological remains using fourteen soil samples extracted from a charcoal kiln with side window located at the Area Ⅰ of Maegokdong. The date of A.D.440${\pm}$15 the analysis of archaeological terrestrial magnetism came up with gives solid evidence, while an archeological chronicle used arrangements of surrounding artifacts because of the absence of remains and assumed uncertainly that a charcoal kiln with side window was from the three kingdom periods. This analysis of archaeological terrestrial magnetism has come to anchor as a main natural scientific analysis because it relatively easily removes pollutants and comes up with highly reliable results owing to its considerably narrow error tolerance of assumed dates.

• Journal of the Korea Society of Computer and Information
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• v.19 no.9
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• pp.45-53
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• 2014

This paper suggests methods to chronicle and track the history of dialogues exchanged among characters to prevent logical errors of a story. As for stories that are long with many characters, especially in full-length novels and co-written stories, cognitive burden is imposed on a writer. If the writer has confused understanding of a character, then a logical error would enter the story. This would compromise completeness and integrity of writing. Against the backdrop, this paper shows how dialogues among characters are chronicled and tracked by using the aforementioned tracking methods through design of a writer support system that relieves a writer's cognitive burden while supporting the writing and through an analysis of existing novels. In addition, we showed the accuracy results of average 68.5% through the performance evaluation of the query used in the dialogue history tracking.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.12 no.1
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• pp.3-24
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• 2001

All human being can't exist by oneself. That is, all human being depend on intimate attachment for their survival. Physical and psychological powers and control functions are prerequisites for one's survival and these serve important functions in interpersonal relationships. There are two kinds of powers and controls. One is positive power which helps one survive effectively. Another one is negative power which disturbs one's effective survival. Introspection is one of the positive power and most important process in understanding oneself and others. Thus, the purpose of this study is to elucidate the physical and psychological meaning of the characteristics of water and based on this meaning, to suggest one way of introspection. The authors reviewed the precept of water which is described in Bible and oriental classical books such as 'the Analects of Confuicus(論語)', 'Tao Te Ching(道德經)', 'Mencius(맹자)', 'Chronicle(史記)', 'The Doctrine of the Mean(中庸)', 'University(大學)'. The author also reviewed 'The History of Three Countries(삼국사기, 삼국유사)', 'The Precept of Religion', and 'the modern poems' which includes the symbolic meaning of water. The results are summarized as follows：1) The flow of water itself is the expression of introspection. Because, water does not flow without filling a puddle. 2) Water is the symbol of 'Immortality'. 3) Water symbolizes 'Modesty and Trust'. 4) Water is the symbol of 'Freedom from Greed'. 5) Water symbolizes 'Goodness and Beauty'. 6) Water symbolizes 'Naturality and Love'. Immortality, Modesty and Trust, Freedom from Greed, Goodness and Beauty, and Naturality and Love are eternal, universal values which all human should seek and achieve. By the process of introspection through the symbolic meaning of water, we can achieve these positive aspects of human mind and spirit.

• Journal of Digital Convergence
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• v.12 no.6
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• pp.559-568
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• 2014

In this age of multimedia, the content of media works as an important factor rather than the format of media. Popular television programs are being supplied cross-border and they go through the process of adaption to the local viewers which is called "formatting". This kind of "formatting" industry had begun in the States, then the Europeans have taken the inactive from 1990s. Since then, it has been settled firmly by the "super-formats". It now has the assumed value of 31 hundred million Euro per year. Especially after 2000s, England has been the center of world broadcasting format industry, and its case has lots of suggestions to Koreans trying to foster the cultural content industry. So this study aims to find the suggestions to Korean content industry by considering the history of broadcast format industry chronically and examining the industrial background of English as a powerful nation of content.

• The Journal of Korean Medical History
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• v.33 no.1
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• pp.43-57
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• 2020

Essence of the Hwangchil tree (黃漆樹 Dendropanax morbiferus H.Lév.) has been used for various purposes, like waxing emperor's armors, covering an astrology board, or, during the Silla Dynasty, burying it to block something bad in the soil symbolically. Essence of the Hwangchil tree was known to have remarkable preservation effects such as waterproofing, damp-proofing, rust-proofing, and moth-proofing as well as not being easily peeled off from even soft surfaces like paper. There is a record in Prescriptions for Epidemic diseases of Cows, Horses, Sheep, and Pigs (牛馬羊猪染疫病治療方), published in 1541, of Hwangchil that is local to Jeju Island being used instead of benzoin (安息香), of burning Hwangchil, and of making cows inhale its smoke to prevent plague among them. Along the same lines, there are records in the Local Chronicle of Tamra (耽羅志) and the Book of Earth Geography (輿地圖書) that identify Hwangchil with benzoin. In Seonghosaseol (星湖僿說), a book written by Lee Yik in around 1760, it is acknowledged that Hwangchil could be medicinal herb. In 2000, Ahn Duk-Kyun registered the roots and branches of Hwangchil tree as 'Boncho' (本草 herbal medicine) in the Pictorial Book of Korean Medicinals (韓國本草圖鑑) and presented the method of taking it for medical purpose. Researchers have suggested that Hwangchil essence as well as diverse parts of the plant such as its roots, branches, leaves, flowers, fruits, and gum have various meaningful medicinal properties. Regarding the history and recent researches of using Hwangchil tree, it has various medicinal probabilities such as, 'dispersing miasma' (辟邪), 'opening holes' (開竅), 'waking the heart' (醒心), 'smoothing spirits' (安神), 'piercing the block' (疎泄), 'removing the old and welcoming the new' (去故生新). This paper contributes ideas about how to expand the uses of Hwangchil Tree.