• Korean Journal of Poultry Science
• /
• v.12 no.2
• /
• pp.83-87
• /
• 1985

This experiment was carried out to identify the chromosomes of silkie. It was many difference from other breeds in morphology and characteristics. In this experiment, chromosomal analysis was used early embryos. In aspect of morphological chromosomes, chromosomal size and shape are similar to other breeds. The chromosomes of silkie were shown to morphlogy as follows. They were identified that chromosome #l and #2 were grouped as submentacentric, ＃3, ＃5 and ＃6 were telocentric ＃4 and ＃7 were acrocentric and ＃8 was metacentric chromosome. Zㆍsex chromosome was shown 5th, W-sex chromosome was 8th to 9th and they were metacentric chromosome, respectively. Each chromosome through the G-banding was shown the 3 dark bands in 1 p2, distinct light band in 1p1, dark band in 2p2, broad light band in 3pl, dark band from centromere and distal part in 4th chromosome and dark band in 5pl. Z-sex chromosome was shown dark at p-arm distal part.

• Korean Journal of Plant Taxonomy
• /
• v.51 no.3
• /
• pp.192-197
• /
• 2021

In the flora of Korea, Carex L. is one of the most species-rich genera. Among nearly 157 Carex taxa, less than 30 have had chromosome numbers reported. We report the meiotic chromosome numbers of eight Carex taxa from Korean populations, which include the first count for C. accrescens Ohwi (n = 37_II) and the first chromosome investigations of Korea populations for three taxa: C. bostrychostigma Maxim. (n = 22_II), C. lanceolata Boott (n = 36_II), and C. paxii Kuk. (n = 38_II). In most species, chromosome counts observed in the study are included in the variation ranges of previous chromosome numbers. However, C. bostrychostigma Maxim. (n = 22_II) and C. planiculmis Kom. (n = 29_II) are assigned new chromosome numbers. Carex is known to have holocentric chromosomes, lacking visible primary constrictions and exhibiting great variance in its chromosome number. Further investigations of the diversity of Carex chromosomes will provide basic information with which to understand the high species diversity of the genus.

• Environmental Mutagens and Carcinogens
• /
• v.11 no.2
• /
• pp.107-117
• /
• 1991

The effect of 3-aminobenzamide (3AB), an inhibitor of poly (ADP-ribose) polymerase, on ethyl methanesulfonate (EMS)-or bleomycin (BLM)-induced DNA repair synthesis and chromosome aberrations was examined during the cell cycle of Chinese hamster ovary (CHO)-K$_1$ cells. The synchronized cells were obtained by using thymidine double block method and mitotic selection method. Three assays were employed in this study: unscheduled DNA synthesis, alkaline elution and chromosome aberrations. 3AB alone did not induce DNA repair and chromosome aberrations in all phases. The post-treatment with 3AB inhibited DNA repair synthesis induced by EMS or BLM in G$_2$ phase, whereas 3AB did not affect chromosome aberrations induced by EMS or BLM in all phases. These results suggest that 3AB aggravates the cell cycle disturbance which occur after DNA damage, and leads to an accumulation of cells at G$_2$ phase, and inhibits DNA repair synthesis, while the effect 3AB on chromosome aberrations may need reevaluated.

• Environmental Mutagens and Carcinogens
• /
• v.18 no.2
• /
• pp.109-115
• /
• 1998

Fluorscence in situ hybridization with chromosome-specific probe has been shown to be a valid and rapid method for detection of chromosome rearrangements induced by chemical and physical agents. This method is useful for quantifying structural aberrations, expecially for stable ones, such as translocation and insertion, which are difficult to detect with conventional method in human lymphocyte. In order to use the FISH method as a biodosimeter for monitoring human population exposed to various chemical and physical agent, baseline level of chromosome rearragement was established. Blood from forty four healthy adults were collected and analysed with whole chromosome-specific probes by human chromosome 1,2 and 4. The frequencies of stable translocation were 2.45 per 100 cell equivalent and those of insertion, color juction, acentric and dicentric were 0.32, 3.28, 0.23 and 0.27 per 100 cell equivalent respectively. The frequencies of chromosome rearragements increased with age in both sexes except for dicenrics. From above result, stable aberrations accumulate with age and it may reflect integrated lifetime exposure of adverse environment.

• Korean Journal of Medicinal Crop Science
• /
• v.18 no.3
• /
• pp.186-190
• /
• 2010

The chromosome number was identified and fluorescence in situ hybridization(FISH) mapping of 5S and 45S rDNAs were conducted for C. minima and C. lanceolata in the genus Codonopsis from Jeju island. In this study, we have confirmed that the somatic metaphase chromosome number determined as 2n=2x=16 was the same as the findings from the previous studies. While the conventional staining method makes it rather difficult to distinguish satellite chromosomes due to high degree of variability, FISH analysis produced the exact number and location of 5S and 45S rDNAs. Both species in the genus Codonopsis have a pair of 5S rDNA and their gene loci were observed on chromosome 3. Although two pairs of 45S rDNAs (one on chromosome 1 and the other on chromosome 8) were identified in both species, the 45S rDNA signals on chromosome 8 in C. minima were significantly weaker than those on chromosome 1. In addition, the 45S rDNA signals on chromosome 1 in C. lanceolata showed that the chromosome is non-homologus. In this study, we have determined cytogenetic characteristics of C. minima and C. lanceolata according to their gene replication patterns.

• Korean Journal of Clinical Laboratory Science
• /
• v.41 no.1
• /
• pp.6-10
• /
• 2009

Ring chromosome occurs when both telomeres of a chromosome are lost and the remaining portion of the chromosome circularizes to re-establish chromosome stability. This abnormal structure shows mitotic instability unlike the normal chromosomes, causing problems during mitosis. Here, we report one case of "chromosome 4 ring syndrome" on a 6-month-old male patient with growth retardation. Ring chromosome, monosomy, dicentric chromosome were shown by conventional chromosome analysis using peripheral blood. Peripheral blood was used and incubated for 72 hours for chromosome analysis. 3 probes (LSI WHS SpectrumOrange/CEP 4 SpectrumGreen, 4p subtelomere probe, 4q subtelomere probe) were used to detect the origin and breakpoint of ring chromosome 4 by FISH (fluorescense in situ hybridization) technique.

• Journal of Plant Biology
• /
• v.33 no.3
• /
• pp.189-196
• /
• 1990

Suspension cell lines have been newly established from the calli derived from the immuature embryo culture of hexapolid (Triticum aestivum var. sicco), tetrapolid (T. durum) and diploid (T. tauchii or Aegilops squarrosa) wheat species. The chromosomal variation in suspension cultured cell lines was examined and old cell line, C82d, established from T. aestivum var. copain was also used. New method using 1-bromonaphthalene for metaphase rapping of suspension cells was developed. Variation in chromosome number was observed among all the suspension lines. Cells with doubled chromosome number and deleted chromosome were also observed. Extensive structural changes in chromosome were found in C82d line. Chromosome aberrations showed loss of chromosome arms and chromosome segment. The mean chromosome number in suspension cells of T. aestivum var. sicco was 40, in C82d line 33, in T. durum 28 and in T. tauchii 14. The stability of chromosome in suspension cells of diploid and tetrapolid wheats was higher than that of hexaploid wheat.

• Korean Journal of Plant Taxonomy
• /
• v.49 no.3
• /
• pp.269-273
• /
• 2019

We report the meiotic chromosome numbers of four Carex taxa from Korean populations. Three are the first reports made on taxa from Korean populations: Carex appendiculata (Trautv. & C. A. Mey.) $K{\ddot{u}}k$. ($n=27_{II}$), C. fernaldiana H. $L{\acute{e}}v$. & Vaniot ($n=33_{II}$), and C. metallica H.$L{\acute{e}}v$. ($n=15_{II}$). Reports on the other species expand the range of variation in the chromosome number within a taxon, C. miyabei Franch. (n = $43_{II}$, $44_{II}$, $45_{II}$). Carex L. (Cyperaceae) consists of more than 2,000 species worldwide and is the most species-rich genus in Korea. The species diversity in the genus has been hypothesized to be associated with the chromosome variation, but chromosome information pertaining to Korean Carex taxa is not well known. This report updates the chromosome number inventory on Korean Carex to 24 out of 180 taxa.

• The Korean Journal of Zoology
• /
• v.7 no.1
• /
• pp.29-32
• /
• 1964

A study on chromosome of leucocytes in blood cultures derived from 6 normal Korean was performed . Exact chromosome counts were carried out on 205 cells in male, 211 in female , of which 86.05% revealed a chormosome mordal number of 46. On the basis of relative chromosome lengths and position of centromeres, the Karyotype that the human chromosomes were classified into 7 groups with 22 airs of autosome and one pair of sex chromosome was determined accoridng to the method of denver report. The chromosome number on metaphase was observed in short term cultures of leucocytes from the peripheral blood of 2 patients with chronic granulocytic leukemia and 1 patient with acute granulocitic leukemia . and the chromosome morpholoogy was also investigated in one acute leukemic patient. In all leukemic cases the leucocytes showed the constant value of 46 in the stem -line of chormosome number. But the frequency of cells with 46 chromosomes appeared in the 3 cases was 67.30% in average with a slightly higher range in hypo-andhyper-diploid chromosome numbers than in normal human, The idiogram analysis did not show any abnormality of chromosome in acute leukemic cells.

• The Korean Journal of Zoology
• /
• v.13 no.1
• /
• pp.15-20
• /
• 1970

The present study was undertaken in an attempt to see if the segregation frequency of a third chromosome was changed by changing the sex chromosome which were free of SD in the second chromosome. The eight genotype males having different sex chromosome constitution each were constructed by appropriate matings and the two standard laboratory stocks of Drosophila melanogaster, e and se were used as the third chromosome recessive markers for the present experiment. The results of the present investigation are given below: 1. The k values which are the proportion of the se third chromosomes recovered among progeny flies from the mating of se/e males to e females were highly signiicantly different among the four genotypes and between the two sexes,and the interaction of genotype and sex was significantly different. Thus the setregation frequency of the se third chromosome in the male, when made heterozygous with the e third chromosome, was dependent upon the sex chromosome constitution. 2. Both of the k(Woman) and the k(man) remains roughly constant among genotypes. 3. The sex ratio o the se progeny class was highly significantly heterogeneous among the four genotypes but it was homogeneous for the e progeny class. 4. The values of the k(man) and the sex ratio of the se progeny class, on the average, were higher than that of the k(Woman) and of the e progeny class, respectively. 5. Those phenomena suggest that some sort of prezygotic selection could be operating such that the combination of the e third chromosome and the Y chromosome tends to be eliminated before fertilization. This tendency argues for a re-examination of the viability estimations of Drosophila melanogaster.