Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)
- Volume 18 Issue 2
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- Pages.109-115
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- 1998
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- 1225-6307(pISSN)
Frequency of Chromosome Aberrations Detected by Fluorescence in Situ Hybridization Using Triple Chromosome-Specific Probes in o Healthy Korean Population
3중 염색체 probe를 이용한 FISH(fluorescence in situ hybridization)기법으로 분석한 정상인의 염색체 이상빈도
Abstract
Fluorscence in situ hybridization with chromosome-specific probe has been shown to be a valid and rapid method for detection of chromosome rearrangements induced by chemical and physical agents. This method is useful for quantifying structural aberrations, expecially for stable ones, such as translocation and insertion, which are difficult to detect with conventional method in human lymphocyte. In order to use the FISH method as a biodosimeter for monitoring human population exposed to various chemical and physical agent, baseline level of chromosome rearragement was established. Blood from forty four healthy adults were collected and analysed with whole chromosome-specific probes by human chromosome 1,2 and 4. The frequencies of stable translocation were 2.45 per 100 cell equivalent and those of insertion, color juction, acentric and dicentric were 0.32, 3.28, 0.23 and 0.27 per 100 cell equivalent respectively. The frequencies of chromosome rearragements increased with age in both sexes except for dicenrics. From above result, stable aberrations accumulate with age and it may reflect integrated lifetime exposure of adverse environment.
Keywords
- Fluorscence in situ hybridization (FISH);
- triple chromosome-specific probe;
- chromosome rearangement;
- PAINT system;
- human population