• BMB Reports
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• 제29권3호
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• pp.241-247
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• 1996

The Philadelphia (Ph) chromosome is the single most intensively studied chromosome alteration characterizing a human malignancy. The specific genetic alteration of chronic myelogenous leukemia (CML) is the formation of the BCR/abl fusion gene in leukemic cells. The presence of the BCR/abl gene has important diagnostic and prognostic implications in CML. The detection of BCR/abl transcripts by reverse transcriptase based polymerase chain reaction (RT-PCR) was investigated in patients with CML in whom the Ph chromosome abnormality was documented by cytogenetic analysis. In a total of 68 CML patient cases, the Ph chromosome was found in 53 cases (77.9%) by cytogenetic analysis. On the other hand, sixty two cases (91.2%) were detected to have BCR/abl gene rearrangement Of these, b3a2 was 44 cases (64.7%) and b2a2 was 17 cases (25,0%). There was one case with both b3a2 and b2a2 (1.5%). Of the fifteen cases of Ph chromosome negative by cytogenetic anlaysis, the BCR/abl gene was observed in nine cases, The results of BCR/abl fusion gene confirmed by the direct sequencing method correlated well with PCR analysis, The amplified PCR products were detected by $1{\times}10^{-5}$ dilutions. In conclusion, PCR technique is sensitive, rapid and relatively simple for a laboratory test in detecting the BCR/abl fusion gene with CML regardless of the result of cytogenetic analysis.

• 한국환경보건학회지
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• 제17권1호
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• pp.110-119
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• 1991

This study was performed to investigate the applicability of 9 chromosome aberration and sister chromatid exchange analysis for the assessment of cytotoxicity and cytogenetic effects of cadmium. Induction of chromosome aberration and sister chromatid exchange in CHO-K1 cells and human peripheral lymphocytes by 2 hour-treatment of CdCl$_{2}$ with various concentrations was observed in relation to their frequencies and types of aberration. The frequency of chromosome aberration in CHO cells treated with CdCl$+{2}$ at G$_{1}$ was increased with dose-dependent manner. When human peripheral lymphocytes were treated with cadmium at G0 and harvested at 72 hours there after, the response was dose-dependent and all the aberrations were also chromatid types. There was no significant increase in frequencies of sister chromatid exchange in both CHO cells and human lymphocytes treated with different concentrations of cadmium. It was suggested that SCE analysis was not a good assessment method for cadmium toxicity.

• 식물분류학회지
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• 제47권4호
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• pp.304-307
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• 2017

We report somatic chromosome numbers for three species belonging to the Euphorbia pekinensis complex distributed in Far East Asia. In E. pekinensis populations distributed in Korea, 2n = 28 and 56 were found, while the Japanese native E. lasiocaula was also found at 2n = 28 and 56 and the Japanese endemic E. sinanensis was found at 2n = 20. Based on the number of chromosomes, E. lasiocaula distributed in Japan supports treatment as a variety of E. pekinensis rather than as a different species, while E. sinanensis should be recognized as a distinct species rather than as a variety of E. pekinensis. In the same populations of E. pekinensis and E. lasiocaula, diploid and tetraploid individuals were found, and the diversity of these chromosome numbers was consistent with the morphological diversity of these populations, suggesting the future evolutionary potential of this species.

• 한국잠사곤충학회지
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• 제43권1호
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• pp.53-54
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• 2001

The chromosome number of Morus bombycis Koidz. and Morus monogolica C.K.Schn. growing wild in the Korea Peninsula is diploid (2n=28) and that of Morus tiliaefolia Makino is hecxaploid (2n=84). The somatic cell division of each species is nomal.

• 한국동물학회지
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• 제34권1호
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• pp.54-58
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• 1991

감돌고기속 어류에는 2종이 알려져 있으며 이들은 모두 한국고유어종이다. 감돌고기 Pseudopugtungia nigra의 핵형분석 결과 diploid chromosome number는 50이었으며, 7쌍의 metacentric, 18쌍의 $_{submeta}$telocentric chromosome으로 구성되어져 잇었다. 가는 돌고기 P. tenuicorpus의 2N은 50이었으며 10쌍의 metacentric, 15쌍의 $_{submeta}$telocentric chromosome으로 구성되어져 있었다.

• 생명과학회지
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• 제12권5호
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• pp.605-609
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• 2002

개의 염색체수는 2n=78이며 염색체의 형태를 보면 submetacentric chromosome metacentric chromosome인 X와 Y를 제외하고 모든 상염색체가 acrocentric chromosome이며 그 크기가 작고 크기의 차이가 나지 않는 염색체들이 많아 G-banding등에 의한 핵형의 표준화가 완전하게 이루어지고 있지 않다. 본 연구fl서는 G-banding high-resolution-banding NOR- banding등의 방법을 이용하여 개의 미소 염색체들에 대한 핵형 및 염색체 동정을 하고자 하였다. C-banding과 high-resolution banding에 의한 그 결과 아직 표준화가 되어 있지 않은 염색체 중 34번, 37번 등의 염색체에서 본 연구 결과가 차이가 있었으나 이는 해석상의 차이로 보이며 NOR-banding에 의한 분석결과 Y염색체와 3쌍의 상염색체에서 존재함을 확인 할 수 있었고 이는 개의 염색체 동정의 지표로 활용할 수 있으리라고 생각된다.

• 한국환경과학회지
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• 제7권5호
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• pp.599-605
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• 1998

The purpose of this work was to examine whether X-Y chromosome dissociation in the primary spermatocytes of mice could be used as an in vivo short-term assaying system that detect environmental mutagens. Four alkylating agents(EMS, MMS, MMC and MNNG) which were known as strong mutagens were administered to BALB/c male mice 3-4 months old. In the control group, the mean frequencies of previously dissociated X and Y chromosomes and autosomes were 7.17% and 2.12%, respectively. Compared to the control group, mutagen-treated groups have no significant differences in dissociation rate of autosomes, while these poops were about 1.2-2.5 times higher in the frequencies of X-Y dissociation. Generally, X-Y dissociation frequency increased consistently with the concentration of mutagens whereas the tendency of autosome dissociation frequency was variable among several mutagens. These results suggest that X-Y dissociation in the primary spermatocytes of mice is applicable as an vivo short-term assaying system for environmental mutagens. There were significantly distinct increase in dissociation of X-Y chromosome in both the hybrid and parents but the X-Y previous dissociation of hybrid appeared higher frequency than BALB /c and wild mice. These results indicate that the factor related to binding X-Y chromosome is specific to strains.

• 한국환경성돌연변이발암원학회지
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• 제7권2호
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• pp.103-111
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• 1987

The effects of LET (linear energy transfer) of radiation on the induction of somatic chromosome mutations or gene mutations of Drosophila melanogaster were studied. For detecting somatic chromosome mutations and gene mutations, Drosophila wing spot system and eye-color spot system were used, respectively. The frequencies of somatic chromosome mutations or gene mutations induced after third instar larval treatment with 23 MeV neutrons, thermal neutrons, X-rays were examined. From these data, the RBE(relative biological effectiveness) values of 23 MeV neutrons relative to X-rays for induction of somatic chromosome mutations or gene mutations were calculated. The present results suggest that high LET radiations are efficient than X-ray in producing not only somatic chromosome mutations but also gene mutations.

• Clinical and Experimental Reproductive Medicine
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• 제21권2호
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• pp.207-214
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• 1994

This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37$^{\circ}C$ for 3 days. And the concentration of colcemid and reincubation time also affected the chromosome morphology. In harvest, chromosome patterns were mainly affected on hypotonic treatment which included treated time and temperature, dropwise of fixative solution, and drying after slide preparation.

• Parasites, Hosts and Diseases
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• 제41권1호
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• pp.41-45
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• 2003

Effects of gamma irradiation on the worm survival and chromosomal aberration of Clonorchis sinensis were studied. The metacercariae irradiated with various amounts of gamma radiation (ranging from 5 Gy to 50 Gy) were fed to rats, and the effects were compared with those of non-irradiated controls. Recovery rates of adult worms in irradiated groups were reduced gradually as increasing of the irradiation doses. No worm was recovered from rats which were fed with 50 Gy irradiated metacercariae. The chromosome number was 2n = 56 in all worms from all experimental groups. However, the groups irradiated with 20 Gy, 25 Gy or 30 Gy showed variations in the chromosome number, depending on different cells in the same individual. Radiation doses used in this study did not appear to induce chromosome aberrations, however, irradiation with 30 Gy showed slightly reduced chromosome size.